• Journal of Oral Medicine and Pain
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• 제44권1호
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• pp.25-30
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• 2019

Purpose: The exact causes of burning mouth syndrome (BMS) is unclear so far. There are many studies to elucidate the relation between oral disease and genetic predisposition. In this study, we first tried to investigate salivary exosomal genetic components that could play an important role for diagnosing and elucidating the progression of BMS. Methods: We compared salivary exosomal micro RNAs (miRNAs) of BMS Patients to those of control using next generation sequencing (NGS). Unstimulated whole saliva from 15 patients with BMS and 10 control subjects were divided into two sets. Isolated exosomes and their total RNAs were subject to NGS for the screening of miRNAs. Results: There were up-regulated 10 exosomal miRNAs (hsa-miR-1273h-5p, hsa-miR-1273a, hsa-miR-1304-3p, hsa-miR-4449, hsa-miR-1285-3p, hsa-miR-6802-5p, hsa-miR-1268a, hsa-miR-1273d, hsa-miR-1273f, and hsa-miR-423-5p) and down-regulated 18 exosomal miRNAs (hsa-miR-27b-3p, hsa-miR-16-5p, hsa-miR-186-5p, hsa-miR-142-3p, hsa-miR-141-3p, hsa-miR-150-5p, hsa-miR-374a-5p, hsa-miR-93-5p, hsa-miR-29c-3p, hsa-miR-29a-3p, hsa-miR-148a-3p, hsa-miR-22-3p, hsa-miR-27a-3p, hsa-miR-424-5p, hsa-miR-19b-3p, hsa-miR-99a-5p, hsa-miR-548d-3p, and hsa-miR-19a-3p) in BMS patients comparing with those of control subjects. Conclusions: We show that there are 28 differential expression of miRNAs between the patients with BMS and those of control subjects. The specific function of indicated miRNAs should be further elucidated.

• Asian Pacific Journal of Cancer Prevention
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• 제14권1호
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• pp.139-143
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• 2013

Patients at the same pathological stage of esophageal cancer (EC) that received the same surgical therapy by the same surgeon may have distinct prognoses. The current study aimed to explore the possibility of differentially-expressed microRNAs (miRNAs) underlying this phenomenon. Samples were collected from EC patients at the same tumor node metastasis (TNM) stage but with different prognoses. Paracancerous normal tissues were taken as controls. The specimens were histopathologically analyzed. Differentially-expressed miRNAs were analyzed using real-time quantitative reverse transcription polymerase chain reaction. Compared with patients with poor prognosis, those with good prognosis exhibited 88 two-fold or more than two-fold increased miRNA fragments and 4 half-decreased miRNAs. The most noticeably up-regulated miRNAs included hsa-miR-31, hsa-miR-196b, hsa-miR-652, hsa-miR-125a-5p, hsa-miR-146b, hsa-miR-200c, hsa-miR-23b, hsa-miR-29a, hsa-miR-186, hsa-miR-205, hsa-miR-376a, hsa-miR-410, hsa-miR-532-3p, and hsa-miR-598, whereas the most significantly-downregulated miRNAs were hsa-let-7e, hsa-miR-130b, and hsa-miR-103. EC patients at same TNM stage but with different prognoses show differentially-expressed miRNAs.

• Molecules and Cells
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• 제43권11호
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• pp.953-963
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• 2020

Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is an infectious disease with multiple severe symptoms, such as fever over 37.5℃, cough, dyspnea, and pneumonia. In our research, microRNAs (miRNAs) binding to the genome sequences of severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory-related coronavirus (MERS-CoV), and SARS-CoV-2 were identified by bioinformatic tools. Five miRNAs (hsa-miR-15a-5p, hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-16-5p, and hsa-miR-196a-1-3p) were found to commonly bind to SARS-CoV, MERS-CoV, and SARS-CoV-2. We also identified miRNAs that bind to receptor proteins, such as ACE2, ADAM17, and TMPRSS2, which are important for understanding the infection mechanism of SARS-CoV-2. The expression patterns of those miRNAs were examined in hamster lung samples infected by SARS-CoV-2. Five miRNAs (hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-221-3p, hsa-miR-140-3p, and hsa-miR-422a) showed differential expression patterns in lung tissues before and after infection. Especially, hsa-miR-15b-5p and hsa-miR-195-5p showed a large difference in expression, indicating that they may potentially be diagnostic biomarkers for SARS-CoV-2 infection.

• 폴리머
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• 제30권3호
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• pp.230-237
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• 2006

단량체로 methyl methacrylate, n-butyl acrylate, 2-hydroxyethyl acrylate와 도막물성 향상 및 가교밀도를 극대화시켜 줄 관능성 단량체인 acetoacetoxyethyl methacrylate (AAEM)를 반응시켜 4원공중합체인 고형분 80%의 아크릴수지 (HSA-98-20, HSA-98-0, HSA-98+20)를 합성하였다. AAEM 성분이 함유된 아크릴수지의 점성도는 $1420\sim5760cps$, 수평균분자량 $2080\sim2300g/mol$, 다분산도 $2.07\sim2.19$ 및 전환율 $88\sim93%$이었다. 고형분 80%인 아크릴수지와 이소시아네이트 경화제를 상온에서 경화시켜 하이솔리드 도료(HSA-98-20C, HSA-98-0C, HSA-98+20C)를 제조하고 도막시편을 제작하여 각종 물성 시험을 수행한 결과, 제조된 하이솔리드 도료내에 AAEM 도입 전후의 도막물성이 비교실험에서 AAEM 도입후에 내마모성과 내용제성이 증진됨으로써 자동차 상도용 도료에의 적용이 가능케 되었다. 또한 점탄성 측정에 의한 도막의 경화거동에서 HSA-98+20C > HSA-98-0C > HSA-98-20C의 순서로 경화가 빨리 진행됨으로써, 유리 전이 온도 값의 증가함에 따라 경화속도가 빨라짐을 알 수 있었다.

• Bulletin of the Korean Chemical Society
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• 제32권7호
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• pp.2222-2226
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• 2011

Amylase is a digestive enzyme that catalyses the starch into sugar. It has been reported that the green tea flavonoid (or polyphenols) (-)-epigallocatechin 3-gallate (EGCG) inhibits human salivary ${\alpha}$-amylase (HSA) and induced anti-nutritional effects. In this study, we performed docking study for seven EGCG-like flavonoids and HSA to understand the interaction mechanism of HSA and EGCG and suggest new possible flavonoid inhibitors of HSA. As a result, EGCG and (-)-epicatechin gallate (ECG) bind to HSA with complex hydrogen bonding interactions. These hydrogen bonding interactions are important for inhibitory activity of EGCG against HSA. We suggested that ECG can be a potent inhibitor of HSA. This study will be helpful to understand the mechanism of inhibition of HSA by EGCG and give insights to develop therapeutic strategies against diabetes.

• 에너지공학
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• 제16권4호
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• pp.202-214
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• 2007

에너지 IT 사업을 보다 효율적으로 수행하기 위해서 뿐만 아니라 전력선 통신망을 기반으로 한 서비스 구현을 효과적으로 추진하기 위해 제시되는 개념이 HSA(Home Service Aggregator)이다. 전력선 통신망 기반의 HSA 사업 모델 개발은 새로운 에너지 분야 사업 영역의 확대와 소비자 중심의 서비스를 통한 신수요 창출의 기회를 제공할 것으로 기대를 모으기에 충분하다고 할 수 있다. 따라서 본 연구에서는 보다 효율적이고 성공적인 HSA사업 모델 개발을 위해 가장 우선적으로 행해져야할 경제성 평가를 에너지 사업자 입장에서 면밀히 분석해 정책 의사 결정에 도움을 주고자 하였다. 분석을 위한 기본 데이터 수집을 위해 총 사업 기간은 2009년도부터 2020년까지로 하였으며, HSA사업 비용의 범위로 장비 비용, 장비 설치 비용, 그리고 운영비용으로 나누어 살펴보았다. 이중 장비 비용 및 장비 설치비용은 MGW와 RMS에 대한 것이고, 운영비용은 인건비, 회선운용비 및 설비 유지 비용으로 구성하였다. 그리고, 투자 대상의 범위는 주거 밀도가 높은 도심지를 대상으로 한 약 900만 수용가로 삼았다. HSA사업을 통해서 얻을 수 있는 중요 편익 원천으로는 원격검침서비스(AMR : Automatic Meter Reading), 인터넷통합빌링 서비스, 통합과금서비스, 인터넷 서비스, 그리고 방범 서비스에 대한 편익을 분석 범주로 설정하여 경제성 평가를 수행하였다. 그리고 전기 안전 편익은 사회적 편익으로 별도로 살펴보아 HSA사업의 정책 결정에 도움을 주고자 하였다. 연구 분석 방법론으로써는 경제성평가 방법론 중 가장 많이 쓰이고 있는 현금흐름할인법을 활용한 순현재가치법을 이용하였다. 연구결과 HSA 사업의 총 투자비는 1조 400여억원으로 산출되었고, 사회적편익을 제외한 총편익은 1조 20여억원이 산출되었다고 2015년까지 투자비를 회수한다고 가정하였을때 각가구로부터 얻어야 할 편익은 월간 가구당 513원으로 분석되었다. HSA의 사업에 대한 ROI는 1에 약간 못미치는 0.954값이 나왔으나, 사회편익 요소인 전기안전 편익이 크기에 이를 모두 편익에 포함한다면 HSA사업의 경제성은 1을 넘게 됨을 확인할 수 있었다.

• 한국응용과학기술학회지
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• 제22권4호
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• pp.371-378
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• 2005

In the previous study, three kinds of monomers and the functional monomer, acetoacetoxyethyl methacrylate (AAEM), which could improve the film property and cross-linkage, were polymerzied into acrylic resin copolymers (HSA-98-20, HSA-98-0, HSA-98+20) containing 80% solid content. In this study, the high-solid coatings(HSA-98-20C, HSA-98-0C, HSA-98+20C) were prepared by the curing reaction between acrylic resins containing 80% solid content and isocyanate at room temperature. Various properties were examined for the film coated with the prepared high-solid coatings. The introduction of AAEM in the coatings enhanced the abrasion resistance and solvent resistance of coatings, which indicated the possible use of high-solid coatings for top-coating materials of automobile. The curing times measured by viscoelastic measurement were 350, 264, and 212 min for HSA-98-20C, HSA-98-0C, and HSA-98+20C, respectively. This shows that the curing times become shorter with increasing $T_g$ values.

• 한국강구조학회 논문집
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• 제26권5호
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• pp.473-483
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• 2014

건축구조기준에 따라 HSA800 강재의 합성기둥 적용시에는 실험 또는 해석적 방법을 통하여 적용의 타당성을 검증해야 한다. 이에 본 연구에서는 합성기둥으로 주로 사용되는 H형강 매입형, 각형강관 및 원형강관 충전형 합성기둥 단면을 대상으로 HSA800 강재를 적용한 단주압축실험을 실시하고, 이를 통하여 축방향 내력 및 건축구조기준의 합성기둥 설계압축강도 설계식 적용의 타당성을 평가하였다. 실험결과 매입형 합성기둥의 경우 HSA800 강재의 설계기준항복강도를 저감없이 사용하기 위해서는 건축구조기준의 설계압축강도 산정식의 조정이 필요한 것으로 나타났으며, 이를 위해 띠철근 간격 조정 및 콘크리트의 유효단면적 사용을 제안하였다. 충전형 합성기둥의 경우에는 각형, 원형충전강관 기둥 모두 별도의 강도 저감이나 설계압축강도 산정식의 조정 없이 사용이 가능할 것으로 판단된다.

• BMB Reports
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• 제39권5호
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• pp.530-536
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• 2006

Thermal conformational changes of human serum albumin (HSA) in phosphate buffer, 10 mM at pH = 7 are investigated using differential scanning calorimetric (DSC), circular dichroism (CD) and UV spectroscopic methods. The results indicate that temperature increment from $25^{\circ}C$ to $55^{\circ}C$ induces reversible conformational changes in the structure of HSA. Conformational change of HSA are shown to be a three-step process. Interestingly, melting temperature of the last domain is equal to the maximum value of fever in pathological conditions, i.e. $42^{\circ}C$. These conformational alterations are accompanied by a mild alteration of secondary structures. Study of HSA-SDS (sodium dodecyl sulphate) interaction at $45^{\circ}C$ and $35^{\circ}C$ reveals that SDS affects the HSA structure at least in three steps: the first two steps result in more stabilization and compactness of HSA structure, while the last one induces the unfolding of HSA. Since HSA has a more affinity for SDS at $45^{\circ}C$ compared to $35^{\circ}C$, It is suggested that the net negative charge of HSA is decreased in fever, which results in the decrease of HSA-associated cations and plasma osmolarity, and consequently, heat removal via the increase in urine volume.

• Journal of Plant Biotechnology
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• 제33권4호
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• pp.233-236
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• 2006

Human serum albumin (HSA) is the most abundant protein in plasma and is the most often used intravenous protein in many human therapies. However, HSA is currently extracted only from plasma because commercially feasible recombinant expression systems are not available. This study attempted to develop an efficient system for recombinant HSA production by chloroplast transformation of tobacco. A HSA cDNA was isolated from a cDNA library constructed with human liver tissue. Chloroplast transformation vectors were constructed by introducing various regulatory elements to HSA regulatory sequences. Vectors were delivered by particle bombardment into leaf explants and chloroplast-transformed plants were subsequently regenerated into whole plants. Southern blot analysis confirmed that the HSA cDNA was incorporated between rps12 and orf70B of the chloroplast genome as designed. Western blot analysis revealed that hyper-expression and increasing the stability of HSA were achieved by modification of the regulatory sequences using the psbA5'UTRs in combination with elements of the 14 N-terminal amino acids of the GFP and the FLAG tag. However, only plants transformed with the vector containing all of these elements were able to accumulate HSA.