• Asian Pacific Journal of Cancer Prevention
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• 제14권6호
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• pp.3837-3841
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• 2013

Objectives: To analyse HPV integration prevalence and genotype distributions in cervical intraepithelial neoplasia (CIN) in east part of China, furthermore to assess preferential sites for common HPV integrations and provide baseline information for cervical abnormality screening and prevention. Methods: Integration of HPV in 113 paraffin-embedded cervical intraepithelial neoplasia samples was assessed using Gencap technology in Key Laboratory of Biotechnologies in BGI-Shenzhen. Results: 64 samples were HPV-integrated and as the cervical lesions increased, the integration rate became higher significantly (P=0.002). Fifteen different HPV genotypes were detected, 14 high-risk (16, 18, 31, 33, 51, 52, 56, 58, 66, 68) and 1 low-risk (11). The most common genotypes were HPV-16, 58, 33, 52, 66, and 56. Thirteen patients had co-integration involving mainly HPV-16 and 58. The frequency of HPV gene disruption was higher in L1 and E1 genes than in other regions of the viral genomes. Conclusion: Some 56.6% of CIN lesions in Qingdao had HPV integrations, and 67.2% of HPV-integrated patients were HPV-16 and 58, more prone to be integrated in younger patients below 45 years old. There exist preferential sites for HPV-16 and HPV-58 integration, and they are more likely to be disrupted in the L1 and E1 loci.

• Asian Pacific Journal of Cancer Prevention
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• 제17권11호
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• pp.4863-4865
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• 2016

Background: Prostatic adenocarcinoma by Prosate cancer (PCa) is the most prevalent cancer and the second cause of cancer-related death among men in the Western world. Human papilloma virus (HPV) may be considered as a preventable risk factor. In this study, we assessed the frequencies of HPV infection in prostatic adenocarcinoma and benign prostatic hyperplasia (BPH) cases in Northeast Mexico. Materials and Methods: A total of 87 paraffin-embedded blocks (from 25 and 62 patients with definite diagnoses of BPH and adenocarcinoma, respectively) were selected and subjected to INNOLiPA HPV Genotyping to detect 28 high- and low-risk HPV types. The rates of infection were compared in the two studied groups. Results: INNOLiPA HPV demonstrated great sensitivity for HPV detection on paraffin-embedded tissue. Global prevalence was 14.9% (13/87). HPV infection was positive in 19.4% (12/62) of patients with adenocarcinoma and 4.0% (1/25) of patients with BPH. HPV-11, which is considered to be low risk, was more prevalent. Interestingly, one patient with BPH and six with prostate cancer showed examples considered to be high risk (HPV-18, -51, -52, and -66). Conclusion: A higher rate of HPV infection among Mexican patients with prostatic carcinoma than among those with BPH was observed. HPV infections may thus contribute to the risk of prostate cancer. Further studies are required to elucidate any roles of HPV infection in prostate disease in Mexico and the effect of prevention and treatment of HPV infection on prostatic adenocarcinoma.

• 여성건강간호학회지
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• 제25권3호
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• pp.285-298
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• 2019

Purpose: The purpose of this study was to identify the impacts of human papillomavirus (HPV) vaccination-related health belief, attitudes toward HPV vaccination, and subjective norms on HPV vaccination intent targeting mothers of elementary school daughters. Methods: The study use a correlative survey design. The subjects of the study were 121 mothers of elementary school daughters located in metropolitan city B. All subjects agreed to participate in the study. The data were collected from September 1 to October 18, 2018, and were analyzed using descriptive statistics, Independent t-test, ANOVA with a post hoc $Scheff{\acute{e}}$ test, Pearson's correlation coefficients, and stepwise multiple regression. Results: Attitude toward HPV vaccination, subjective norms, vaccination plans for their children, and mother's vaccination status were significant factors influencing HPV vaccination intention. These factors accounted for 72% of the HPV vaccination intention. Conclusions: This study showed that factors affecting the intention of mothers of elementary school daughters to vaccinate against HPV were HPV vaccination attitudes, subjective norms, vaccination plans for their children, and mother's vaccination status. The biggest influencing factor was HPV vaccination attitudes. Therefore, in order to encourage mothers of elementary school daughters to vaccinate against HPVs, national-level policies should be implemented to create a social atmosphere of positive attitudes toward HPV vaccinations that also emphasizes how easy it is to get vaccinated against HPV. Based on this, mothers of elementary school daughters should be encouraged to have them vaccinated against HPV, thus contributing to improving the HPV vaccination rates of elementary school girls.

• 기본간호학회지
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• 제22권2호
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• pp.158-168
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• 2015

Purpose: The purpose of this study was to investigate knowledge of cervical cancer, HPV and intention for vaccination in Korean soldiers on active duty. Methods: Participants were 494 army men aged 18 to 26 serving in D area. Knowledge of cervical cancer was measured using the 8 item scale developed by Han et al. (2007) and knowledge of HPV was measured using the 13 item scale developed by Pitts et al. (2009). Collected data were analyzed with t-test, $x^2$-test, and ANOVA. Results: Mean scores for cervical cancer and HPV knowledge were $6.03{\pm}1.52$ and $8.15{\pm}1.75$ respectively and vaccination intention was 75.6%. HPV vaccination intention had significant differences depending on knowledge about cervical cancer (${\chi}^2=12.76$, p=.001), and HPV (${\chi}^2=9.00$, p=.003), education (${\chi}^2=12.24$, p=.002), sexual intercourse after first meeting (${\chi}^2=11.79$, p=.003), and sexual education in the military service (${\chi}^2=12.27$, p=.001). Conclusion: When planning education program for men in military service, it is necessary to consider include content to increase knowledge of cervical cancer and HPV.

• BMB Reports
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• 제36권4호
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• pp.349-353
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• 2003

To explore the application of DNA chip technology for the detection and typing of Human Papillomavirus (HPV), the HPV6, 11, 16 and 18 gene fragments were isolated and printed onto aminosilane-coated glass slides by a PixSys 5500 microarrayer as probes to prepare the HPV gene chips. HPV samples, after being labeled with fluorescent dye by restriction display PCR (RD-PCR) technology, were hybridized with the microarray, which was followed by scanning and analysis. The experimental condition for preparing the HPV gene chips was investigated, and the possibility of HPV genotyping using gene chips was discussed. The technique that was established in this study for preparing HPV gene chips is practical. The results of the present study demonstrated the versatility and inspiring prospect of using this technology to detect and genotype HPV.

• Asian Pacific Journal of Cancer Prevention
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• 제14권5호
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• pp.3177-3181
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• 2013

Background: Human papillomavirus (HPV) infection is the main causes of cervical cancer in women worldwide. The goal of the present study was to determine the prevalence and distribution of HPV genotypes in women from Saudi Arabia. Recently, several HPV detection methods have been developed, each with different sensitivities and specificities. Methods: In this study, total forty cervical samples were subjected to polymerase chain reaction and hybridization to BioFilmChip microarray assessment. Results: Human papillomavirus (HPV) infections were found in 43% of the specimens. The most prevalent genotypes were HPV 16 (30%) HPV 18 (8.0%) followed by type HPV 45, occurring at 5.0%. Conclusion: Our finding showed the HPV infection and prevalence is increasing at alarming rate in women of Saudi Arabia. There was no low risk infection detected in the tested samples. The BioFilmChip microarray detection system is highly accurate and suitable for detection of single and multiple infections, allowing rapid detection with less time-consumption and easier performance as compared with other methods.

• 생명과학회지
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• 제19권2호
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• pp.234-240
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• 2009

부산지역의 유흥업소 종사여성을 대상으로 HPV DNA chip 시험을 통하여 HPV 유병율 및 유전자형 분포를 조사하였다. 총 660건의 자궁경부도찰 검체로부터 258건(양성율 39.1%)이 검출되었으며, 20대에서 가장 높은 유병율을 나타내었다. 양성자의 유전자형 분포는 고위험군 유전자형의 경우 HPV-16 (15.9%), -53 (10.2%), -58 (7.7%), -18 (5.2%)순이었고, 저위험군 유전자형의 경우 HPV-70 (10.4%), -6 (4.1%), -11 Journal of Life Science 2009, Vol. 19. No. 2 239 (2.0%)순이었다. 특이하게 고위험군 감염여성의 분포비(62.0%)가 저위험군(14.7%) 및 고/저위험군 혼합감염(12.0%)보다 4배 가까이 높았다. 전체 양성자중 단일유형 감염자는 175건(67.8%)이고 혼합유형 감염자는 83건(32.2%)이었다. 본 연구 결과 밝혀진 부산지역 유흥업소 종사여성에서 많이 발견되는 HPV 유전자형 분포의 다양성은 국내 도입된 자궁경부암 예방백신의 효과 예측 및 국내 특성에 맞는 다가백신 개발의 기초 자료가 될 수 있을 것으로 사료된다.

• 대한의생명과학회지
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• 제22권3호
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• pp.98-106
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• 2016

Investigation of human papillomavirus (HPV) in archival formalin-fixed paraffin-embedded (FFPE) material is important for understanding cervical carcinogenesis. The objective of the present study was to identify the high risk HPVs (HR-HPVs) using HPV E6/E7 mRNA testing from archival tissues in cervical cancer and the relation to HR-HPVs genotypes in paired cervical exfoliated cells. HPV E6/E7 mRNA testing and DNA chip testing were performed in 79 paired cervical FFPE tissues and exfoliated cells from women with histologically confirmed squamous cell carcinoma and adenocarcinoma. Overall agreement in HR-HPVs detection from FFPE samples and cytology samples were 98.5% in HPV 16, 100% in HPV 18, HPV 31, HPV 33, HPV 58, HPV 66, and HPV 68. Type-specific agreement between FFPE samples and cytology samples was 89.1% in HPV positive, 93.5% in HPV 16 and more than 70% in the other HR-HPVs. In conclusion, HR-HPVs were reliably detected in paired FFPE and cytology samples with some variation in type-specific detection.

• 대한의생명과학회지
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• 제21권2호
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• pp.69-76
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• 2015

Cervical cancer is the third most common cancer in women worldwide and there is a significant association between human papillomavirus (HPV) infection and cervical cancer. Certain HPV groups, labeled high-risk (HR) HPV groups, are strongly associated with malignancies of the human cervix. HPV prevalence and genotype distribution were analyzed using the REBA $HPV-ID^{(R)}$ (YD Diagnostics, Yongin, Korea) assay based on the reverse blot hybridization assay (REBA) with a total of 324 liquid-based cytology samples from women in Western Shandong Province, East China and results were compared with cytological diagnosis. Most of the HPV genotypes that were detected in high-grade cervical lesions were HR-HPV genotypes such as HPV 16, 18, 33, 53, and 58. The prevalence of these HR-HPV genotypes increased in high-grade cervical lesions. However, from low- to high-grade cervical lesions, the ability to detect LR-HPV genotypes decreased. Additionally, in general, the single HPV genotype infection rate increases in proportion to the severity of the lesion. The study findings suggest that a currently available preventive vaccine against HPV 16 and 18 may have limited effectiveness for prevention of all HPV infection in this province. Finally, based on these findings, these data could guide national or regional vaccination programs in the Western Shandong Province of East China to substantially reduce the burden of cervical lesions.

• 한국콘텐츠학회논문지
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• 제14권8호
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• pp.307-318
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• 2014

본 연구는 계획된 행위이론을 적용하여 여중생 딸을 둔 어머니의 HPV(human papillomavirus) 백신 접종 의도를 파악하고, 관련 요인을 알아보기 위함이며, G광역시에 소재한 여중학교 4개교의 어머니 198명을 대상으로 하였다. 연구 도구로 HPV 백신 접종에 대한 태도, 주관적 규범, 지각된 행위 통제는 Askelson 등이 개발한 도구를 사용하였으며 자료 수집 기간은 2012년 9월 17일부터 24일까지였고, 수집된 자료는 SPSS 18.0 Program을 사용하였다. 본 연구 결과 여중생 어머니들의 HPV 백신 접종 의도는 비교적 높았지만, 6개월 이내에 접종하겠다고 한 어머니는 29.8% 뿐이었고, 어머니들이 생각하는 HPV 백신 최적 접종 연령이 평균 17.74세였다. 대상자의 HPV 백신 접종 의도는 태도, 지각된 행위 통제, 주관적 규범과 통계적으로 유의한 상관관계가 있었고, HPV 백신 접종에 대한 태도, 주관적 규범, 지각된 행위 통제가 접종의도의 중요한 영향 요인임이 확인되었으며, 대상자의 HPV 백신 접종 의도를 84.3%를 설명하였다. 그러므로 어머니들이 HPV 백신 접종에 대해 긍정적으로 생각하고, 백신 접종을 쉽고, 용이하게 지각하도록 격려하는 홍보 및 사회적 분위기 조성이 필요하리라 사료된다.