• Title/Summary/Keyword: HPLC.

Search Result 6,038, Processing Time 0.029 seconds

Quantitative and Pattern Recognition Analyses for the Quality Evaluation of Cimicifugae Rhizoma by HPLC

  • Fang, Zhe;Moon, Dong-Cheul;Son, Kun-Ho;Son, Jong-Keun;Min, Byung-Sun;Woo, Mi-Hee
    • Bulletin of the Korean Chemical Society
    • /
    • v.32 no.1
    • /
    • pp.239-246
    • /
    • 2011
  • In this study, quantitative and pattern recognition analysis for the quality evaluation of Cimicifugae Rhizoma using HPLC/UV was developed. For quantitative analysis, three major bioactive phenolic compounds were determined. The separation conditions employed for HPLC/UV were optimized using ODS $C_{18}$ column ($250{\times}4.6mm$, $5{\mu}M$) with isocratic elution of acetonitrile and water with 0.1% phosphoric acid as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 323 nm. These methods were fully validated with respect to the linearity, accuracy, precision, recovery, and robustness. The HPLC/UV method was applied successfully to the quantification of three major compounds in the extract of Cimicifugae Rhizoma. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of twelve reference samples corresponding to five different species of Cimicifugae Rhizoma and seventeen samples purchased from markets. The results indicate that the established HPLC/UV method is suitable for the quantitative analysis and quality control of multi-components in Cimicifugae Rhizoma.

Absence of Tocotrienol Form of Vitamin E in Purple Perilla (Perilla frutescens var. acuta Kudo) Seeds Confirmed by Comparative Analysis Using HPLC and GC (GC 및 HPLC 비교분석에 기초한 차조기 종실내 tocotrienol 부재의 평가)

  • Lee, Young-Sang;Kim, Min-Kyoung
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.53 no.spc
    • /
    • pp.115-120
    • /
    • 2008
  • Lipid soluble vitamin E consists of tocopherols and tocotrienols depending upon double bonds in phytyl side chains attached to chromanol ring. Recent reports on antioxidative, anticancer, and cholesterol-lowering effects of tocotrienols have increased researches and commercialization of tocotrienols. Purple perilla (Perilla frutescens var. acuta Kudo) has been reported as a plant containing tocotrienols along with tocopherol forms of vitamin E based upon normal phase HPLC analysis. To confirm the existence or absence of tocotrienol form of vitamin E in purple perilla, comparative analysis using HPLC, GC/FID, and GC/MSD has been conducted for 14 purple perilla genetic accessions collected from Korea and Japan. Normal phase HPLC analysis showed ${\alpha}-$, ${\beta}-$, ${\gamma}-$, and ${\delta}-tocopherols$ along with peaks with retention times quite similar to ${\beta}-$ and ${\gamma}-tocotrienols$. Same purple perilla samples, analysed by GC exhibited ${\alpha}-$, ${\beta}-$, ${\gamma}-$, and ${\delta}-tocopherols$ quantitatively equivalent to HPLC results. However, no peaks for ${\beta}-$ and ${\gamma}-tocotrienols$ could be observed and unknown two peaks of similar retention times with ${\beta}-$ and ${\gamma}-tocotrienols$ were identified not corresponding tocotrienols by GC/MSD. These results suggest the absence of tocotrienol form of vitamin E in purple perilla as well as the necessity of using GC-based qualitative and quantitative vitamin E analysis to avoid misinterpretation of peaks with similar retention times as tocotrienol isomers when analysed by an HPLC.

Identification of Proteins in Egg White Using Ion Exchange Cartridge and RP-HPLC (이온교환 카트리지와 RP-HPLC를 이용한 난백 단백질의 확인)

  • Kim, Hyun Moon;Kim, Ah Reum;Lee, Chang Soo;Kim, In Ho
    • Korean Chemical Engineering Research
    • /
    • v.50 no.4
    • /
    • pp.713-717
    • /
    • 2012
  • Approximately forty proteins in egg white have been widely studied for their functional properties. To develop a procedure of separation for pure and non-altered proteins from egg white, purification study was conducted to isolate lysozyme, ovotransferrin, and ovalbumin. Ion exchange cartridge can selectively separate proteins from egg white, and reversed-phase HPLC (RP-HPLC) could identify separated proteins. Proteins in egg white were purified by HI trap ion exchange cartridge SP and Q with buffers pH 8.0 and 5.2. C18 column (Phenomenex, USA) was used for RP-HPLC analysis and isocratic mobile phase was used with acetonitrile (ACN)/distilled water (DW)/trifluoroacetic acid (TFA) in the ratio of 50/50/0.1. Comparing the retention times of standards in RP-HPLC experiments showed that ovotransferrin, ovalbumin, and lysozyme in egg white were eluted successively in the RP-HPLC column after the pretreatment in SP and Q ion exchange cartridges.

Sesamin and Sesamol Contents of Korean Sesame Oils Determined by HPLC Method and Villavecchia-suarez Color Reaction of the Oils (국내 산지별 참기름의 HPLC에 의한 Sesamin, Sesamol의 함양과 Villavecchia-suarez 정색반응)

  • 이철원;곽인신
    • Journal of environmental and Sanitary engineering
    • /
    • v.11 no.1
    • /
    • pp.1-8
    • /
    • 1996
  • In the present study the sesamin and sesamol contents of the sesame extracts prepared from nine provinces in Korea were determined HPLC method. A comparative test was also carried out using the Villavecchia-suarez test, the red colored reaction for the sesamol and sesamol derivatives. The contents of sesamin and sesamol of the sesame oils from each area by the HPLC method were 0.57-0.78% and 0.010-0.023%, respectively, and the paralled results were obtained by the Villavecchia-suarez test and the HPLC method. The average content of the sesamin was $0.68{\pm}0.074%$ by the HPLC method and the average absorbance of the Villavecchia-suarez test was $0.56{\pm}0.034$. The contents of sesamol from sesame oil by the HPLC method and the Villavecchia-suarez test were so low that it was not possible to correlate with the sesamin contents. The contents of sesamol from the sesame oil produced in Kyeong-gi and Jeon-nam provinces were $0.010{\pm}0.002%$ and $0.023{\pm}0.004%$, respectively.

  • PDF

The Rapid Detection of Antioxidants from Safflower Seeds (Carthamus tinctorius L.) Using Hyphenated-HPLC Techniques (Hyphenated-HPLC 기술을 활용한 홍화씨의 항산화 성분 분석)

  • Kim, Su-Jin;Kim, Sang-Min;Kang, Suk-Woo;Um, Byung-Hun
    • Korean Journal of Food Science and Technology
    • /
    • v.42 no.4
    • /
    • pp.414-419
    • /
    • 2010
  • Hyphenated-HPLC techniques combine the separation power of HPLC with the structural and bioactivity information provided by NMR, ESI/MS, and an on-line antioxidant screening system. The major advantages over the traditional off-line techniques are rapidity and efficiency. In this study, we used hyphenated HPLC techniques including online HPLC-ABTS, LC-NMR, and LC-MS todirectly identify the major antioxidants of safflower (Carthamus tinctorius L.) seeds. The results demonstrated that the major antioxidant compounds from on-line HPLC-ABTS analysis were identified as 8'-hydroxyarcgenin-4'-O-$\beta$-D-glucoside, N-(p-coumaroyl) serotonin, and N-feruloylserotonin. Among them, N-feruloylserotonin accounted for almost 50% of the ABTS radical scavenging activity of the total extract. The results demonstrate that HPLC hyphenated techniques can be used to rapidly screen and structurally identify antioxidants from crude plant extracts.

Isolation and HPLC Analysis of Timosaponin A III from Rhizomes of Anemarrhena asphodeloides $B_{UNGE}$ (지모의 유효성분 분리 및 HPLC 정량 분석)

  • Kim, Geum-Soog;Park, Chang-Kie;Seong, Jae-Duck;Kim, Hyun-Tae;Han, Sang-Ik;Kwack, Yong-Ho
    • Korean Journal of Medicinal Crop Science
    • /
    • v.7 no.1
    • /
    • pp.45-50
    • /
    • 1999
  • Timosaponin A III, an active and major compound, was isolated from rhizomes of Anemarrhena asphodeloides. The quantitative analysis of timosaponin AIII was performed by a high performance liquid chromatographic(HPLC) method using ELSD and the useful extraction method for HPLC analysis was examined as well. This HPLC method can be utilized as the standard analytical method for the evaluation of the quality of Anemarrhena rhizoma in the steps of breeding and cultivation. Additionally, the HPLC analysis method can be useful for the evaluation of the quality of Anemarrhena rhizoma sold as a traditional medicine in current markets.

  • PDF

Optimal HPLC Condition for Simultaneous Determination of Anthocyanins in Black Soybean Seed Coats (검정콩 함유 안토시아닌의 동시분석을 위한 최적 HPLC 분석 조건)

  • Choung, Myoung-Gun
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.53 no.4
    • /
    • pp.359-368
    • /
    • 2008
  • Black soybean has been widely utilized as foods and oriental medicinal materials. The pigmentation in the seed coat of black soybean is due to accumulate anthocyanins in the epidermis palisade layer. The anthocyanins of black soybean seed coat are considered as a parameter of quality evaluation of black soybean. Therefore, the purpose of this study was to investigate the most suitable HPLC condition for simultaneous determination of anthocyanins in black soybean seed coats extracts. The efficient HPLC analytical condition of D3G, C3G, and Pt3G contained extracts of black soybean seed coats was developed. The gradient elution employed a $250\;mm\;{\times}\;4.6\;mm$ i.d. YMC-pak ODS-AM 303 column. The gradient system was used two mobile phases. A gradient elution was performed with mobile phase A, consisting of 5% aqueous formic acid, and mobile phase B, comprising 5% formic acid - acetonitrile, and delivered at a flow rate of 0.7 mL/min as follows: $0{\sim}35\;min$, $90%\;A{\sim}60%\;A$; 36 min, 90% A; 46 min, 90% A. The UV-VIS. detection wavelength was set at 520 nm. The limit of detection (LOD) for D3G, C3G, and Pt3G were under 10 ng/mL.

Identification of Ginseng Saponin and Quantitative Determination of $Ginsenoside-Rb_1$ from Crude Drug Preparation Drink (생약복방제 드링크중 인삼 saponin의 확인 및 $Ginsenoside-Rb_1$의 분리 정량)

  • 최강주;고성룡
    • Journal of Ginseng Research
    • /
    • v.14 no.2
    • /
    • pp.112-116
    • /
    • 1990
  • As a part of studies on the quality control of crude drug preparation drinks, ginseng saponins were identified by HPLC. Ginsenoside-Rb1 was determined quantitatively by HPLC. Ginsenoside MeOH/H2O(65:35:10, v/v) on Si-gel plate. Ginsenoside-Rb1 content determined by HPLC on Lichrosorbtract drinks was 57.5-70.4% compared to the content in the red ginseng extract.

  • PDF

Comparative Analysis of Fritillaria Species by HPLC (Fritillaria속 식물의 HPLC에 의한 비교분석)

  • Kwon, Yong-Soo;Hong, Sung-Sook;Kim, Chang-Min
    • Korean Journal of Pharmacognosy
    • /
    • v.32 no.4 s.127
    • /
    • pp.307-310
    • /
    • 2001
  • In order to investigate alkaloid contents in different Fritillaria species, we analyzed 17 Fritillaria species by HPLC using prederivatization method. The contents of alkaloid $fr.5(11-deoxo-6-oxo-5{\alpha},6-dihydrojervine)$, delavinone and delavine were analyzed and compared.

  • PDF

Chemiluminescence immunochromatographic analysis for the quantitative determination of algal toxins

  • Pyo, Dongjin;Kim, Taehoon
    • ALGAE
    • /
    • v.28 no.3
    • /
    • pp.289-296
    • /
    • 2013
  • For the quantitative detection of algal toxin, microcystin, a chemiluminescence immunochromatographic assay method was developed. The developed system consists of four parts, chemiluminescence assay strip (nitrocellulose membrane), horse radish peroxidase labeled microcystin monoclonal antibodies, chemiluminescence substrate (luminol and hydrogen peroxide), and luminometer. The performance of the chemiluminescence immunochromatographic assay system was compared with high performance liquid chromatography (HPLC) detection. The detection limit of chemiluminescence immunochromatographic assay system is several orders of magnitude lower than with HPLC. The chemiluminescence immunochromatography and HPLC results correlated very well with the correlation coefficient ($r^2$) of 0.979.