• Journal of Nutrition and Health
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• v.21 no.1
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• pp.47-60
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• 1988

This study was performed to investigate the effect of dietary levels on protein metabolism in eight healthy Korean adult females. The 20-day metabolic study consisted of 2 day adaptation period and three 6-day experimental. Three experimental diets were low protein (LP : protein 44g), high protein(HP : protein 85g) and high animal protein (HAP : protein-84g). The apparent absorption and balance on nitrogen were significantly higher in high protein than in low protein diet. Nitrogen, absorption rate was about 75% for low protein and about 85% for high protein intake. The mean values of nitrogen balance were -1.28% for low protein and 0.78% for high protein diet. All the subjects were in negative nitrogen balance at the low protein intake while they were in positive nitrogen balance at the high protein intake. The mean daily urinary nitrogen excretion increased with increased level of protein intake. Urea nitrogen was the largest part of the urinary nitrogen. The ratio of urea nitrogen to total urinary nitrogen increased significantly for 79 to 85% as protein intake was doubled.

• Korean Journal of Pharmacognosy
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• v.34 no.4 s.135
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• pp.269-273
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• 2003

The leaves of Acanthopanax sessiliflorus Seem. (Araliaceae), which is native plant to Korea, have not been studied yet on triterpene constituents. Three 3,4-seco-lupane triterpenoids (Compound I-III) were isolated from the MeOH extract of this plant using Diaion HP-20P, silica gel and ODS column chromatographes. Based on physicochemical and spectroscopic data, the chemical structures of these compounds were identified as follows ; chiisanogenin (Compound I), chiisanoside (Compound II), $22{\alpha}-hydroxychiisanoside$ (Compound III).

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.20 no.6
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• pp.793-798
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• 2011

In this study, it was grasped to the flow characteristics of cutting fluid injected by nozzle installed in high pressure holder for avoiding chip curling occurred during machining process. And for avoiding chip curling, the possibility of elimination under various chip conditions was checked. Consequently, the highest discharging pressure and velocity was shown in 150 of nozzle inflow angle. Also as nozzle outlet diameter is small, the pressure and velocity of injected flow are high. Moreover, It could be confirmed that width and thickness of chip have no direct effect on chip elimination and it is achieved by torque generated by injected cutting fluid.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2002.10a
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• pp.153-153
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• 2002

수돗물의 염소소독 과정에서 부식질의 초기 분해생성물인 MX(Mutagen X)는 수돗물의 전체 돌연변이유발성의 최고 60%까지 차지하는 것으로 알려져 있다. 그러나 존재하는 농도는 수 ng/L로 정량분석을 위해서는 resin을 사용하여 10 ~ 20L의 물을 농축하여야 한다. 이 연구에서 MX의 농축에 사용된 resin은 XAD-4와 XAD-7HP의 1:1 mixture이었으며, 사용 전에 Soxhlet extractor를 사용하여 ethyl acetate와 methanol로 각각 12시간씩 세척하였다. 15L의 물 시료는 37%의 염산으로 pH 2에 맞춘 후 직경 2cm의 유리관에 15cm의 높이로 채워진 resin을 40mL/min의 유속으로 통과시켰다. 유리관에 잔류하는 물은 질소가스로 대부분 불어 제거한 후 ethyl acetate 200mL를 가해 1mL/min의 유속으로 흘려주어 MX를 용출시킨 후 회전증발기로 5mL의 부피까지 농축시키고 10% H$_2$SO$_4$ in MeOH 0.1mL를 가하여 6$0^{\circ}C$에서 methyl ester화하였다. 과포화된 $Na_2$SO$_4$ 수용액 0.3ml를 가한 후 MTBE 0.3mL로 추출하고 GC/ECD로 분석하였다. MX의 회수율은 60% 이상이었으며 일부수돗물 시료에 대해 분석하였다.

• Journal of radiological science and technology
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• v.20 no.1
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• pp.85-90
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• 1997

Although many useful arfticles appear in the journals published in Korea, they are not always cited by researchers mainly due to absence of efficient searching system. The author made a program with 4 predefined filtering forms to detect published articles rapidly and accurately. The program was coded using database management system CA-Clipper VER 5.2. I used 486DX-II (8 Mbyte Ram, VGA, 560 Mbyte Hard Disk), desk-jet printer(HP-560k), and MS-DOS VER 5.0. I inputed twenty articles in the journal of Korean Society Radiotechnological Technology. And this program test for retrive article and made database.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1998.11a
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• pp.130-130
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• 1998

Two novel compounds SSC1 and SSC2 were isolated from culture broth produced from the strain of Stereum hirsutum by using of YM broth. They were isolated through HP-20 column chromatography, silica gel column chromatography and preparative HPLC, successively. The molecular formulas of SSC1 and SSC2. were determined as C$\sub$15/H$\sub$22/O$_3$ by high resolution EI -mass. The chemical structures of SSC1 and SSC2 were determined as sesquiterpenoid compounds by spectroscopic analysis of UV, IR, $^1$H NMR, $\^$13/C NMR, DEPT, HMQC and HMBC spectrum.

• Food Science and Biotechnology
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• v.14 no.1
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• pp.89-93
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• 2005

Squalene synthase (SQS) inhibitors were screened from various plants and food extracts. Effective SQS inhibitor was purified from fermented soybean paste using ethanol extraction, HP-20 column chromatography, ethyl acetate extraction, silica gel column chromatography, and crystallization. Through UV spectrometry, $^1H$ NMR, $^{13}C$ NMR, and mass spectrometry, SQS inhibitor was identified as daidzein with molecular mass of 254 and molecular formula of $C_{15}H_{10}O_4$. Daidzein showed $IC_{50}$ value of 50 nmol/L against SQS, confirming its potential as therapeutic agent for hypercholesterolemia.

• Proceedings of the Korean Nuclear Society Conference
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• 1997.10a
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• pp.627-632
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• 1997

FLECHT-SEASET 실험을 이용하여 냉각재상실사고시 Reflood에 대한 TRAC-PF1 전산코드의 예측 능력을 평가하였다. FLECHT-SEASET 실험 장치는 3.657m(12 ft) 높이 161개 전열 봉으로 이루어 져 있으며, 다양한 재관수율, 계통압력, 초기 피복재온도, 재관수온도 노심내 반경방향 출력분포 둥의 조건에 따라 수행된 실험이다. TRAC-PF1은 비균질 비평형 이상유동 열수력(Nonhomogeneous Non-equilibrium Two-Fluid Hydrodynamic)모델을 사용하고 원자로 압력용기는 3차원으로 모델할 수 있는 최적전산코드로서, 이 평가 계산에는 HP Version이 사용되었다. 본 연구에서는 재관수율 변화에 따라 달라지는 연료봉 최대 피복재온도와 Quench 시간에 대한 TRAC-PF1 전산코드의 예측 능력을 중점적으로 평가하였다. 계산 결과 TRAC-PF1은 최대 피복재온도는 약 20-100$^{\circ}$K 낮게, Quench 시간은 실험치와 비교하여 약 40-150초 정도 늦게 예측하는 것으로 나타났는데, 재관수율이 낮을수록 최대피복재 온도는 낮게, Quench 시간은 늦게 예측하는 경향을 보이고 있다. 또한 재관수율이 3 in/sec 이상에서 노심 상부가 일찍 Quenching 되는 것으로 계산되는데, 이는 노심상부 열전달 Regime의 부적절한 계산이 원인으로 보인다.

• Journal of the Korean Society of Tobacco Science
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• v.24 no.2
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• pp.94-100
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• 2002

Cyclodextrin glucanotransferase from Bacillus stearothemophilus and Bacillus macerans synthesized maltol and ethyl maltol monoglucoside, with a series of its maltooligo-glucosides by transglycosylation with dextrin as a donor, and maltol or ethyl maltol as an acceptor. The monoglucoside formed from reaction mixture of maltol or ethyl maltol by the successive actions of Bacillus stearothemophilus cyclodextrin glucanotransferase and Rhizopus glucoamylase was isolated by Diaion HP-20 column and silica gel column chromatography. The structure of the isolated monoglucoside was identified as maltol-$\alpha$-D-glucoside and ethyl maltol-$\alpha$-D-glucoside, respectively, by FAB-MS, UV, $^1$H-NMR, $^{13}$ C-NMR spectra and products by hydrolysis with acid, $\alpha$ - and $\beta$ -glucosidases.

• YAKHAK HOEJI
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• v.32 no.1
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• pp.6-13
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• 1988

A gas chromatographic method is described for the determination of volatile free acids(VFAs) in body fluids. VFAs were trace enriched from body fluids by liquid-solid extraction using Chromosorb P as the solid sorbent and ether as the eluant. The enriched VFAs were injected in splitless injection mode onto a HP-20M fused silica capillary column. The flame ionization detector was used as the detector. The present method was applied to the profiling of VFAs in body fluids from patients suffering from the infectious disease, hepatitis. The VFAs concentrations were high in saliva of hepatitis patients and isobutyric acid detected in sera of hepatitis patients compared to healthy subjects.