• Korean journal of applied entomology
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• v.54 no.4
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• pp.289-294
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• 2015

Striped flea beetle, Phyllotreta striolata (SFB) and turnip sawfly, Athalia rosae ruficornis (TSF) are two economically important sporadic pests in radish fields on Jeju island. The response of adult SFB and TSF to a mixture of aggregation pheromone, (+)-(6R,7S)-himachala-9,11-diene and host plant volatile, allyl isothiocyanate (HAI), as well as to yellow and blue sticky traps was examined in radish fields. Adult SFB was more attracted to the sticky trap with HAI, regardless of the color of the sticky trap; however, adult TSF was more attracted on the yellow sticky trap than blue, and no effect of HAI was observed. The adult SFB and TSF can be effectively monitored using yellow sticky traps placed 10 cm above the plant canopy. SFB and TSF had 3 and 5 peak times in a year, respectively. The first peak occurred in the middle of March for SFB and mid-late of April for TSF. We expect that the results of the present study can facilitate minimizing the damage caused by the two important pests in commercial radish fields.

• Korean journal of applied entomology
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• v.47 no.2
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• pp.141-147
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• 2008

Male adults of bean bug, Riptortus clavatus Thunberg (Heteroptera: Alydidae), release aggregation pheromone (AP) attracting both sexes of adult and nymphs, which its egg parasite, Ooencyrtus nezarae (Hymenoptera: Encyrtidae) exploits the pheromone to find host. The AP consists of three components; (E)-2-hexenyl (Z)-3-hexenoate (E2HZ3H), (E)-2-hexenyl (E)-2-hexenoate (E2HE2H), and tetradecyl isobutyrate (TI). We analyzed composition of the pheromone components of bean bugs from different geo graphical locations of Korea and Japan. The attractiveness of different blends of AP components to R. clavatus was also tested in the fields in Jinju, Korea and in Kumamoto, Japan. Composition ratios (E2HZ3H: E2HE2H:TI) of the AP of Jinju and Iksan populations were 1:1.4:0.2 and 1:0.8:0.2, and those of Tsukuba and Kumamoto populations were 1:2.8:0.2 and 1:1.5:0.1, respectively. In field tests, traps baited with ratio of 1:1:1 (E2HZ3H:E2HE2H:TI=16.7:16.7:16.7mg/rubber septum) and 1:1:0.5(E2HZ3H:E2HE2H:TI= 20:20:10mg/rubber septum) attracted significantly greater number of adult bugs than that of 1:5:1 (E2HZ3H:E2HE2H:TI=7.1:35.7:7.1mg/rubber septum).

• Korean journal of applied entomology
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• v.56 no.4
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• pp.331-338
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• 2017

In the present study, we investigated the effects of two herbicide-resistant genetically modified rice (GM rice) varieties, Milyang 204 and Iksan 483, recently developed in Korea on non-target insects and a spider. No difference in host preferences of the English grain aphid Sitobion avenae and the brown planthopper Nilaparvata lugens were observed between GM rice and non-GM rice. Wolf spider Pirata subpiraticus, feeding on N. lugens reared on GM rice or non-GM rice, revealed no significant difference in body weight. P. subpiraticus, fed with N. lugens reared on Milyang 204, showed survival rates similar to that in P. subpiraticus fed with N. lugens reared on non-GM rice. However, P. subpiraticus feeding on N. lugens reared on Iksan 483 demonstrated significantly lower survival rates than that in P. subpiraticus feeding on N. lugens reared on Milyang 204 or non-GM rice. In addition, when larvae of the western honeybee Apis mellifera were supplied with Iksan 483 pollen, a significantly longer pupal period occurred, as compared with that of A. mellifera supplied with pollen of Milyang 204 or non-GM rice. As GM rice has negative effects on P. subpiraticus, which is an important predator in agricultural ecosystems, and on A. mellifera, which plays important roles in pollination and honey production, additional studies on risk assessment of GM rice should be conducted before releasing newly developed herbicide-resistant GM rice to the agricultural environment.

• Biomedical Science Letters
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• v.16 no.2
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• pp.119-122
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• 2010

Interleukin-1${\beta}$ $(IL-1{\beta})$ is one of the key proinflammatory cytokines and it plays an important role for the antimycobacterial host defense mechanisms. In this study, we examined Mycobacterium tuberculosis (MTB)-stimulated induction of IL-1${\beta}$ and evaluated the associated signal transduction pathways. In PMA-differentiated THP-1 cells, MTB infection increased mRNA expression of IL-$1{\beta}$ in a dose-dependent manner. The expression of IL-1${\beta}$ mRNA began to be induced at 1.5 h after infection, and induced expression of IL-1${\beta}$ was retained for 48 h after MTB infection. The increase in expression of IL-1${\beta}$ caused by MTB was reduced in cells treated with Ro-31-8425 (an inhibitor of PK$C{\alpha}$, ${\beta}I$, ${\beta}II$, ${\gamma}$, ${\varepsilon}$) or PD98059 (an inhibitor of MEK1), meanwhile, pre-treatment with $G\ddot{o}6976$ (an inhibitor of $Ca^{2+}$ dependent PK$C{\alpha}$ and PK$C{\beta}I$) or Rottlerin (an inhibitor of PK$C{\delta}$) has no effect on MTB-induced expression of $IL-1{\beta}$ mRNA. These results show that the expression of $IL-1{\beta}$ mRNA caused by MTB may be mediated via MEK1 and PKC isoforms including PK$C{\beta}II$, $PKC{\gamma}$, or $PKC{\varepsilon}$. Further studies are required to determine whether other PKC isoforms $(PKC {\eta},\;{\theta},\;{\varepsilon},\;and\;{\lambda}/{\iota})$, except $PKC{\delta}$, $PKC{\alpha}$, and $PKC{\beta}I$, are also involved in $IL-1{\beta}$ mRNA expression after mycobacterial infection.

• BMB Reports
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• v.28 no.6
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• pp.556-561
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• 1995

The purpose of this study was to establish an in vitro culture method of tumor-specific T cells, and determine the efficacy of the cultured tumor-specific cytotoxic T-lymphocytes (CTL) as an agent of anti-tumor immunotherapy against a murine lymphoma, TIMI.4. Tumor-specific T-lymphocytes derived from C57BL/6 mice (thy-1.2) immune to TIMI.4 were activated by in vitro stimulation with the irradiated TIMI.4 cells, and expanded by restimulation with TIMI.4 in the presence of the concanavalin A-stimulated rat spleen culture supernatant, and splenic antigen-presenting cells. In vitro restimulation enhanced markedly the proportion of $CD8^+$, a predominant surface marker of CTL and the cytotoxic activity in the cultured immune T cell population. The resulting TIMI.4-specific T cells were adoptively transferred into nude mice. The tumor cells residing in the host after 7 days of adoptive transfer to B6.PL (thy-1.1) mice were quantified by use of an antibody directed to the thy-1.2 allele. The TIMI.4 cells in the recipient nude mice were decreased in a dose-dependent manner. Anti-tumor activity of the TIMI.4-specific T cells was also demonstrated by a survival test, where the tumor-bearing nu/nu mice which received the activated T-cells survived about 30% longer than the control mice which received the tumor cells alone. These suggest that adoptive transfer of TIMI.4-specific T cells could be a candidate for effective therapy of the murine lymphoma.

• BMB Reports
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• v.53 no.6
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• pp.329-334
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• 2020

Inflammasomes are cytosolic, multiprotein complexes that act at the frontline of the immune responses by recognizing pathogen- or danger-associated molecular patterns or abnormal host molecules. Mesenchymal stem cells (MSCs) have been reported to possess multipotency to differentiate into various cell types and immunoregulatory effects. In this study, we investigated the expression and functional regulation of NLR Family Pyrin Domain Containing 3 (NLRP3) inflammasome in human umbilical cord blood-derived MSCs (hUCB-MSCs). hUCB-MSCs expressed inflammasome components that are necessary for its complex assembly. Interestingly, NLRP3 inflammasome activation suppressed the differentiation of hUCB-MSCs into osteoblasts, which was restored when the expression of adaptor proteins for inflammasome assembly was inhibited. Moreover, the suppressive effects of MSCs on T cell responses and the macrophage activation were augmented in response to NLRP3 activation. In vivo studies using colitic mice revealed that the protective abilities of hUCB-MSCs increased after NLRP3 stimulation. In conclusion, our findings suggest that the NLRP3 inflammasome components are expressed in hUCB-MSCs and its activation can regulate the differentiation capability and the immunomodulatory effects of hUCB-MSCs.

• Asian Journal of Turfgrass Science
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• v.24 no.1
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• pp.9-15
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• 2010

During 2008~2009 winter season, yellow patch and gray snow mold occurred on turfgrass plants in golf courses in Kangwon and Jeonbuk provinces, respectively. The fungi associated with the diseases were identified as Rhizoctonia cerealis Van der Hoeven and Typhlua incarnata Lasch ex Fr., based on the morphological characteristics of hyphae and sclerotia. R. cerealis and T. incarnata were pathogenic to most turfgrass and crop species tested. R. cerealis infected crown, stem and leaf tissue of the host plants, and the symptom was light yellow circular patch. Individual infected leaf near the margin of patch developed red color first and finally turn brown. The symptoms caused by gray snow mold pathogen are water-soaked spots, and became a watery soft rot. Infection parts became yellow and then turned brown followed by death of the whole plant. White mycelia were developed on higher petioles, leaves, and on soil where these plant parts lay, and black sclerotia of variable size and shape formed in the mycelial mass. All isolates tested were pathogenic on most turfgrass and crop plants, and significantly different in aggressiveness. Disease severity increased with longer snow cover days on target plants, suggesting that disease severity was expressed over snow cover days. There were significant differences in disease severity among the graminious species, and among cultivars within each species, indicating varying levels of susceptibility to R. cerealis and T. incarnata.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.34 no.12
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• pp.1087-1092
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• 2010

A micro 3D-PTV system has been constructed using a single camera system. Two viewing holes were created behind the object lens of the microscopic system to construct a stereoscopic viewing image. A hybrid recursive PTV algorithm was used. A concept of epipolar line was adopted to eliminate many spurious candidates. Three-dimensional velocity vector fields were obtained by calculating the three-dimensional displacements of particles that were identified as being identical. The system consists of a laser light source (Ar-ion, 500 mW), one high-definition camera ($1028{\times}1024$ pixels, 500 fps), a circular plate with two viewing holes, and a host computer. The performance of the developed algorithm was tested using artificial images. The characteristic of the vector recovery ratio was investigated for the particle numbers. A micro backward-facing step channel ($H{\times}h{\times}W:\;36{\mu}m{\times}70{\mu}m{\times}3000{\mu}m$) was measured using the developed measurement system. The results were in good qualitative agreement with other results.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.40 no.9
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• pp.815-820
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• 2016

An elastic polymer (e.g., PDMS) blended with EFG particles is a promising conductive composite for fabricating soft sensors that can detect an object's deformation up to or more than 50%. Here, we develop large-strain, sprayable soft sensors using a mixture of PDMS and EFG particles, which are used as a host elastomer and electrically conductive particles, respectively. A solution for a conductive composite mixture is prepared by the microwave-assisted graphite exfoliation, followed by ultrasonication-induced fragmentation of the exfoliated graphite and ultrasonic blending of PDMS and EFG. Using the prepared solutions for composite and pure PDMS, 1-, 2-, and 3-axis soft sensors are fabricated by airbrush stencil technique where composite mixture and pure PDMS are materials for sensing and insulating layers, respectively. We characterize the soft strain sensors after investigating the effect of PDMS/EFG wt% on mechanical compliance and electrical conductance of the conductive composite.

• Sijohaknonchong
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• v.20
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• pp.75-96
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• 2004

A pavilion is located in the place with beautiful scenery, which maximizes the poetical features of nature. So a scholar in the Chosun Dynasty had the dream of possessing it. This trends of the Chosun scholar resulted in producing lots of literary works related with the pavilion. In this thesis, I researched the characters of Pavilion Si-Jo in accordance with the function of pavilion. First, the pavilion, which is surrounded by the beautiful scenery, is a good place to see the beauty of nature. It is needless to walk around to see the scenery thanks to its open structure. So the feeling in the pavilion Si-Jo is distinguished from other sightseeing Si-Jos: those show the way of seeing the scenery walking here and there, but this shows the way of seeing the scenery only just looking up, looking down, and looking in the distance. Second, the pavilion functions as an academic place. In this case, it can be said that it functions like a lecture hall. It is more effective to study in the pavilion than in the closed structure, a lecture hall. Furthermore, the scholar studying in the pavilion understand the principle of nature with seeing the beautiful mountain and river, and it can be a place for growing a vast-flowing spirit. This kind of poetry has been handed down with the Chinese poetry, and the character who operated the pavilion often appears in the work. Third, the pavilion functions as a space for a fraternity or poetry circles. The poetic exchange was conducted by host of pavilion. which is the key ingredient for organizing a poetry circles. Finally, the pavilion functions as an entertainment. Although it is a space for an entertainment, there is no deviation as a man of novel birth. If the pavilion has the feature of closeness, there are sometimes works which shows the deviation of them.