• Title/Summary/Keyword: HORTICULTURAL CROP

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First Report of Leptosphaerulina australis Isolated from Soil in Korea

  • Li, Weilan;Back, Chang-Gi;Lee, Seung-Yeol;Ten, Leonid N.;Jung, Hee-Young
    • The Korean Journal of Mycology
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    • v.46 no.4
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    • pp.369-374
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    • 2018
  • The fungal strain KNU16-004 was isolated from a field soil sample collected in Seoul. The isolate was identified as Leptosphaerulina australis based on morphological characterization and phylogenetic analysis using the internal transcribed spacer (ITS), large subunit (LSU) rDNA regions, and ${\beta}-tubulin$ (Tub2). This is the first report of Leptosphaerulina australis in Korea.

Growth and Flowering of Calla (Zantedeschia elliottiana cv. 'Black Magic') with various mulching materials and GA3 treatments (멀칭재료와 GA3처리에 따른 유색칼라(Zantedeschia elliottiana cv. 'Black Magic')의 생육 및 개화반응)

  • Nam, Chun-Woo;Yoo, Dong-Lim;Kim, Su-Jeong;Suh, Jong-Teak;Paek, Kee-Yoeup;Lee, Sang Gyu
    • Korean Journal of Agricultural Science
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    • v.41 no.4
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    • pp.283-290
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    • 2014
  • This experiment had been conducted to determine optimal culture conditions for the growth and cut flowers of Calla (Zantedeschia 'Black Magic' 'Golden Affair') in highlands. Tubers of calla 'Black Magic' was treated with 0, 100, and $200mg{\cdot}L^{-1}$ concentration of $GA_3$. ABA content in tubers and roots were twice higher ($20-25pmol{\cdot}mL^{-1}$) in control than in $GA_3$ treated tubers and roots. ABA contents in tubers was not consistent but ABA contents in roots increased with increasing $GA_3$ concentration. However ABA is in inverse proportion to GA. It was mean that GA of high contents was ABA of low contents. Calla 'Golden Affair' was treated with different mulching materials (Control, black PE, Reflective film, Rice hull) and $GA_3$ concentrations (0, 50, 100 and $200mg{\cdot}L^{-1}$). It was largest in the length of flower at mulching of reflective film, Rice hull and it was greatest in the number of cut flowers at the treated with $200mg{\cdot}L^{-1}$ $GA_3$, Therefore the proper condition was at $200mg{\cdot}L^{-1}$ $GA_3$ with reflective film mulching.

Inhibitory Effect of Acetylshikonin from Roots of Lithospermum erythrorhizon on LDL Oxidation and FPTase Activity (지치뿌리로부터 분리한 Acetylshikonin의 LDL 산화 저해활성과 FPTase 저해활성)

  • Kim, Geum-Soog;Jeong, Tae-Sook;Kwon, Byoung-Mok;Kim, Young-Ok;Cha, Seon-Woo;Song, Kyung-Sik;Bek, Nam-In
    • Journal of Applied Biological Chemistry
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    • v.52 no.4
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    • pp.221-225
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    • 2009
  • Lithospermum erythrorhizon has been known well as one of traditional medicine for fever reduction, detoxication, and blood circulation improvement. This study was carried out to isolate biological active compounds from roots of Lithospermum erythrorhizon and to investigate their low density lipoprotein (LDL) antioxidant and anticancer activities. The hexane extract of Lithospermi Radix has been separated on silica gel chromatography and a naphthoquinone pigment compound 1 has been isolated. The structure of the compound 1 has been identified by spectroscopic technique, including MS and NMR, as acetylshikonin (1). Acetylshikonin showed significantly inhibitory activity on $Cu^{2+}$-induced human LDL oxidation with $IC_{50}$ value of $8.8\;{\mu}M$ and obvious anticancer effect by inhibiting farnesyl:protein transferase (FPTase) activity with $IC_{50}$ value of $23\;{\mu}M$, which suggested that acetylshikonin might be useful for the treatment of atherosclerosis and cancer.

High-performance liquid chromatography analysis of phytosterols in Panax ginseng root grown under different conditions

  • Lee, Dong Gu;Lee, Jaemin;Kim, Kyung-Tack;Lee, Sang-Won;Kim, Young-Ock;Cho, Ik-Hyun;Kim, Hak-Jae;Park, Chun-Gun;Lee, Sanghyun
    • Journal of Ginseng Research
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    • v.42 no.1
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    • pp.16-20
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    • 2018
  • Background: The Panax ginseng plant is used as an herbal medicine. Phytosterols of P. ginseng have inhibitory effects on inflammation-related factors in HepG2 cells. Methods: Phytosterols (e.g., stigmasterol and ${\beta}$-sitosterol) in the roots of P. ginseng grown under various conditions were analyzed using high-performance liquid chromatography. The P. ginseng roots analyzed in this study were collected from three cultivation areas in Korea (i.e., Geumsan, Yeongju, and Jinan) and differed by cultivation year (i.e., 4 years, 5 years, and 6 years) and production process (i.e., straight ginseng, red ginseng, and white ginseng). Results: The concentrations of stigmasterol and ${\beta}$-sitosterol in P. ginseng roots were 2.22-23.04 mg/g and 7.35-59.09 mg/g, respectively. The highest concentrations of stigmasterol and ${\beta}$-sitosterol were in the roots of 6-year-old P. ginseng cultivated in Jinan (82.14 mg/g and 53.23 mg/g, respectively). Conclusion: Six-year-old white ginseng and white ginseng cultivated in Jinan containing stigmasterol and b-sitosterol are potentially a new source of income in agriculture.

In vitro grown thickened taproots, a new type of soil transplanting source in Panax ginseng

  • Kim, Jong Youn;Kim, Dong Hwi;Kim, Young Chang;Kim, Kee Hong;Han, Jung Yeon;Choi, Yong Eui
    • Journal of Ginseng Research
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    • v.40 no.4
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    • pp.409-414
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    • 2016
  • Background: The low survival rate of in vitro regenerated Panax ginseng plantlets after transfer to soil is the main obstacle for their successful micropropagation and molecular breeding. In most cases, young plantlets converted from somatic embryos are transferred to soil. Methods: In vitro thickened taproots, which were produced after prolonged culture of ginseng plantlets, were transferred to soil. Results: Taproot thickening of plantlets occurred near hypocotyl and primary roots. Elevated concentration of sucrose in the medium stimulated the root thickening of plantlets. Senescence of shoots occurred following the prolonged culture of plantlets. Once the leaves of plantlets senesced, the buds on taproots developed a dormant tendency. Gibberellic acid treatment was required for dormancy breaking of the buds. Analysis of endogenous abscisic acid revealed that the content of abscisic acid in taproots with senescent shoots was comparatively higher than that of taproots with green shoots. Thickened taproots were transferred to soil, followed by exposure to gibberellic acid or a cold temperature of $2^{\circ}C$ for 4 mo. Cold treatment of roots at $2^{\circ}C$ for 4 mo resulted in bud sprouting in 84% of roots. Spraying of 100 mg/L gibberellic acid also induced the bud sprouting in 81% roots. Conclusion: Soil transfer of dormant taproots of P. ginseng has advantages since they do not require an acclimatization procedure, humidity control of plants, and photoautotrophic growth, and a high soil survival rate was attained.