• 한국약용작물학회:학술대회논문집
• /
• 2009.05a
• /
• pp.137-138
• /
• 2009

• 한국약용작물학회:학술대회논문집
• /
• 2009.05a
• /
• pp.127-128
• /
• 2009

• 한국약용작물학회:학술대회논문집
• /
• 2009.05a
• /
• pp.109-110
• /
• 2009

• Plant Biotechnology Reports
• /
• v.5 no.3
• /
• pp.283-287
• /
• 2011

In order to produce centellosides from whole plant cultures of Centella asiatica (L.) Urban, we evaluated the synergistic effects of thidiazuron (TDZ) and methyl jasmonate (MJ) on whole plant growth and centelloside production. After 4 weeks of treatment with 0.025 mg/L of TDZ coupled with 0.1 mM MJ, the production of made-cassoside and asiaticoside from whole plant cultures was estimated to be 2.40- and 2.44-fold, respectively, above that of MJ elicitation alone. When whole plants were treated with a growth regulator and an elicitor, the growth of whole plants, as compared to the controls, did not differ. Additionally, total phytosyterol content in the leaves of whole plants co-treated with MJ and TDZ was 1.08-fold greater than those of MJ alone. These results demonstrate that combined treatments not only stimulate the accumulation of centellosides in the leaves but also inhibit the reduction of phytosterol levels caused by MJ elicitation.

• Natural Product Sciences
• /
• v.16 no.3
• /
• pp.153-158
• /
• 2010

To fine out the anti-inflammatory activities of the C. fragile. and its mechanism were investigated in macrophages induced by Peptidoglycan (PGN). Treatments of macrophages with 100 ug/ml of ethanol extract of Codium fragile (EECF) inhibited PGN-induced IL-6, NO and PGE2 production in a dose-dependent manner as well as expression of iNOS and COX-2. EECF inhibited PGN-induced extracellular signal-regulated kinase (ERK) 1/2, JNK 1/2 and p38 MAPK phosphorylation, which suggests that EECF inhibits IL-6 and NO secretion by blocking MAPKs phosphorylation. These findings may help elucidate the mechanism by which EECF modulates RAW 264.7 cell activation under inflammatory conditions.

• Journal of Ginseng Research
• /
• v.41 no.4
• /
• pp.444-449
• /
• 2017

The development of molecular markers is one of the most useful methods for molecular breeding and marker-based molecular associated selections. Even though there is less information on the reference genome, molecular markers are indispensable tools for determination of genetic variation and identification of species with high levels of accuracy and reproducibility. The demand for molecular approaches for marker-based breeding and genetic discriminations in Panax species has greatly increased in recent times and has been successfully applied for various purposes. However, owing to the existence of diverse molecular techniques and differences in their principles and applications, there should be careful consideration while selecting appropriate marker types. In this review, we outline the recent status of different molecular marker applications in ginseng research and industrial fields. In addition, we discuss the basic principles, requirements, and advantages and disadvantages of the most widely used molecular markers, including restriction fragment length polymorphism, random amplified polymorphic DNA, sequence tag sites, simple sequence repeats, and single nucleotide polymorphisms.

• Korean Journal of Agricultural and Forest Meteorology
• /
• v.21 no.3
• /
• pp.158-166
• /
• 2019

Implementing proper land management techniques, such as selecting the best crops and applying the best cultivation techniques at the farm level, is an effective way for farmers to adapt to climate change. Also it will be helpful if the farmer can get the information of agro-weather and the growth status of cultivating crops in real time and the simulated results of applying optional technologies. To test this, a system (web site) was developed to produce agro-weather data and crop growth information of farms by combining agricultural climate maps and crop growth modeling techniques to highland area for summer-season Chinese cabbage production. The system has been shown to be a viable tool for producing farm-level information and providing it directly to farmers. Further improvements will be required in the speed of information access, the microclimate models for some meteorological factors, and the crop growth models to test different options.

• The Korean Journal of Pesticide Science
• /
• v.20 no.1
• /
• pp.41-46
• /
• 2016

Bacterial fruit blotch (BFB) caused by Acidovorax avenae subsp. citrulli is defective disease to watermelon cultivated areas. To control of BFB, we investigated control efficiency to use commercial antibacterial pesticides. Growth inhibition zone on medium were formed as oxolinic acid WP and oxytetracycline WP. Control efficacy of four anti-bacterial pesticides on seed and seedling stage were performed. As a results, oxytetracyclin WP is shown over 90% control efficiency on seed and acibenzolar-S-methyl + mancozeb WP shown over 90% control efficiency on seedling stage Hot-water treatment method could be possible to reduced infection rate on seed. The conditions of hot-water treatments are $50{\sim}55^{\circ}C$ on 20~30 minutes. These results suggested that the methods were helpful watermelon seedling nursery to control of the bacterial fruit blotch by A. avenae subsp. citrulli.

• Korean journal of applied entomology
• /
• v.62 no.4
• /
• pp.355-363
• /
• 2023

In addition to causing direct feeding damage to a variety of greenhouse crops, Frankliniella occidentalis also inflicts indirect harm by facilitating the transmission of the tomato spotted wilt virus. Historically, the prevention of F. occidentalis infestations has relied heavily on pesticide use. However, this approach has led to significant side effects in agricultural ecosystems, including the development of pest resistance and challenges in effective prevention. In response to these issues, research has been directed towards identifying alternative substances that circumvent the tolerance developed against chemical pesticides. Extracts from sixty-seven medicinal plants were prepared by soaking them in water for 24 hours at room temperature. These extracts were then applied to adult F. occidentalis, with particular attention to moutan extract treatment. This treatment demonstrated a 100% insecticidal effect on the first day. The moutan extract, specifically, was prepared using 50% ethanol, after which the ethanol and water were removed via a rotary evaporator. The resultant product was then lyophilized into a powder and used after being diluted with water. In indoor experiments, a 40% diluted solution was sprayed onto F. occidentalis, exhibiting a 100% insecticidal effect 24 hours post-treatment. Furthermore, a pot test indicated a 78% insecticidal effect on the first day of application. Ongoing research includes the analysis of active substances that demonstrate exceptional insecticidal properties and the conduct of on-site validation tests. The application of the aforementioned extract is anticipated to be effective in the prevention of F. occidentalis infestations.

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 1999.04a
• /
• pp.48.2-48
• /
• 1999