• 한국식물병리학회지
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• 제14권1호
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• pp.62-67
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• 1998

Barley yellow mosaic virus (BaYMV-HN) occurring Haenam area was isolated by mechanical inoculation onto barley cultivars, purification and production of antibody. BaYV-HN were purified from infected plants a filamentous virus with 13 nm in diameter and 250∼300 nm and 500∼650 nm in length. Specific antibody made by injecting the purified virus to the muscle of a rabbit. In gel-diffusion tests antibody to BaYMV-HN did not make spur with tow Japanese BaYMV isolates BaYMV-II-1 or BaYMV-III. BaYMV-HN showed the symptom of yellowing and necrosis in host plants. Mechanical inoculation tests with Japanese barley cultivars showed that BaYMV-HN infected New Golden, Akagi Nijo and Tosan Kawa 73, but did not infect Amagi Nijo, Haruna Nijo, Ishukushirazu (ym3), Misato Golden (Ym1), Kashimamugi, Joshushiro Hadaka and Mokusekko 3 (ym1). In Korean barley cultivars, some of the naked barleys which are Hinssalbori, Kinssalbori, Saessalbori and Saechalssalbori were not infected by BaYMV-HN. However, it infected all the covered barley cultivars and the beer barley cultivars. BaYMV-HN had two RNAs, RNA 1 (7.6 Kb) and RNA 2 (3.5 Kb), and one coat protein (33 KDa).

• 한국버섯학회지
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• 제21권3호
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• pp.126-131
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• 2023

목질진흙버섯 HN00K9균사체 액체종균을 대두에 배양한 대두(PMS)의 조단백질 함량은 41.99%로 HN00K9 균사체 배양하지 않은 대두(UCS) 38.61%보다 높은 단백질 함량이 검출되었다. PMS와 UCS에서 16종의 유리 아미노산이 검출되었으며 총 아미노산함량은 PMS에서 39,963 mg/100 g으로 UCS의 36,817 mg/100 g보다 8% 이상 증가되었다. 특히 glutamic acid는 PMS에서 7,413 mg/100 g로 전체 아미노산 중 가장 많은 18.5%로 나타났다. 총 polyphenol 함량은 PMS에서 2.66 mg GAE/g로 UCS 1.45 mg GAE/g보다 45%이상 높게 검출되었으며 PMS는 33.3%의 DPPH radical 소거 능을 보여 UCS의 11.5%보다 3배 증가한 높은 항산화 활성을 보였다. 따라서 본 연구의 PMS는 높은 단백질함량과 항산화 활성이 강화된 기능성 식품소재로 활용할 수 있을 것으로 기대된다.

• 청정기술
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• 제25권1호
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• pp.33-39
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• 2019

활성탄(activated carbon, A.C)은 휘발성 유기화합물(volatile organic compounds, VOCs) 제거를 위해 가장 많이 사용되고 있지만 흡/탈착 시 열화현상으로 인한 화재위험성, 잦은 교체 주기로 인한 비용 부담, 수분에 의한 성능 저하 등의 문제점을 가지고 있다. 이러한 문제들을 해결하기 위하여 소수성 제올라이트 흡착제가 연구되고 있다. 본 연구에서는 소수성 개질법 중 하나인 수증기처리 및 산 처리를 통해 $NH_4{^+}Y$-zeolite를 소수성 Y-zeolite로 합성하여 높은 표면적, 열적 안정성과 습도저항성을 확보하고자 하였다. Y-zeolite와 개질된 Y-550-HN, Y-600-HN, Y-650-HN의 흡착성능은 23, 38, 77, $61mg\;g^{-1}$으로 나타났으며, 소수성 개질 정도를 확인할 수 있는 지표인 Si/Al ratio 변화를 XRF 분석으로 확인하였다. 그 결과, Y-zeolite를 개질하였을 때 흡착성능이 증진되었고, Si/Al 비는 Y, Y-550-HN, Y-600-HN, Y-650-HN 순으로 각각 3.1765, 6.6706, 7.3079, 7.4635 임을 확인하였다. 반면에 높은 열처리 온도에 의한 구조적 결정화가 성능 저하에 영향을 미칠 수 있음을 확인하였다. 반면에 Y-zeolite의 최적 열처리 온도가 존재하며, 이와 같은 최적 개질 조건 연구는 높은 내구성과 안정성을 갖는 흡착제를 제조할 수 있는 조건으로써 향후 활성탄을 대체할 수 있을 것으로 판단하였다.

• 미생물학회지
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• 제50권1호
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• pp.33-39
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• 2014

대전일원의 유류오염 지역의 토양으로부터 원유를 단일 탄소원으로 이용하는 총 144균주를 순수분리 하였고, 이중 유화능과 성장률 그리고 표면장력활성이 가장 우수한 한 균주를 최종 선별하여 형태 및 생리 생화학적 특성을 조사하고 16S rRNA 염기서열을 분석을 통해 Pseudomonas sp. HN37이라 명명하였다. 최종 선별된 Pseudomonas sp. HN37는 여러 종류의 지방족 탄화수소와 3,5-dinitrosalicylic acid와 2,4-dichlorophooxyacetic acid를 단일 탄소원으로 이용하여 성장하였다. 그리고 이 균주는 암피실린과 클로람페니콜 항생제와 Ba, Cr, Li, Mn 중금속에 대해 강한 내성을 갖고 있었고, pH 6.0-9.0과 $30^{\circ}C$에서 성장능과 표면장력활성, 그리고 유화능이 가장 우수한 것으로 확인되었다. 또 Pseudomonas sp. HN37는 1% (v/v) 원유 농도와 1%(w/v) NaCl에서 최대 유화능과 표면장력활성을 나타내었고, LB배지에서 배양 15시간 후에 표면장력활성이 62 dyne/cm에서 27 dyne/cm까지 감소하였다.

• Molecules and Cells
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• 제24권1호
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• pp.69-75
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• 2007

Alzheimer's disease is a neurodegenerative disorder associated with progressive loss of cognitive function and memory. Amyloid beta peptide ($A{\beta}$) is the major component of senile plaques and is known to exert its cytotoxic effect mainly by producing $H_2O_2$. Vascular endothelial growth factor (VEGF) is elevated in the cerebrospinal fluid (CSF) and brain of AD patients, and $H_2O_2$ is one of the factors that induce VEGF. Therefore, we tested whether $A{\beta}$ might be responsible for the increased VEGF synthesis. We found that $A{\beta}$ induced the production of $H_2O_2$ in vitro. Comparison of the amount of $H_2O_2$ required to induce VEGF synthesis in HN33 cells and the amount of $H_2O_2$ produced by $10{\mu}M\;A{\beta}_{1-42}$ in vitro suggested that a toxic concentration of $A{\beta}$ might induce VEGF synthesis in these cells. However, toxic concentrations of $A{\beta}$ failed to induce VEGF synthesis in several cell systems. They also had no effect on antioxidant enzymes such as glutathione peroxidase, catalase, and peroxiredoxin in HN33 cells. $Cu^{2+}$, $Zn^{2+}$ and $Fe^{3+}$ are known to accumulate in the brains of AD patients and promote aggregation of $A{\beta}$, and $Cu^{2+}$ by itself induces synthesis of VEGF. However, there was no synergistic effect between $Cu^{2+}$ and $A{\beta}_{1-42}$ in the induction of VEGF synthesis and $Zn^{2+}$ and $Fe^{3+}$ also had no effect on the synthesis of VEGF, alone or in combination with $A{\beta}$.

• 한국통신학회논문지
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• 제33권1B호
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• pp.8-15
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• 2008

CR(Cognitive Radio)은 우선 사용자 네트워크와 공존함으로써 주파수 효율을 극대화할 수 있는 기술이다. 이러한 CR 기술의 가장 우선해야 될 과제는 우선 사용자 시스템에 대한 보호이다. 이를 위해 CR 네트워크는 주기적으로 우선 사용자의 주파수 사용을 탐지하고 우선 사용자 시스템에 간섭을 주지 않기 위해 통신 파라미터를 조절하게 된다. 하지만 CR 네트워크의 특성상 스펙트럼 검출을 통해서도 발견되지 않는 우선 사용자인 HN(Hidden Node)가 존재하게 된다. 이러한 HN가 사용하는 무선 자원을 CR 네트워크에서는 유휴자원이라 판단함으로써 우선 사용자에게 간섭을 주는 문제가 발생하며, 이는 CR 네트워크의 올바른 운용을 방해하는 요인으로 작용한다. 따라서 CR 네트워크의 안전하고 올바른 운용을 위해서는 HN 문제를 해결할 수 있는 방법이 절실히 요구된다. 본 논문에서는 이러한 HN 문제를 해결할 수 있는 방법을 제시하며, 이에 대한 성능 평가를 수행하였다.

• 원예과학기술지
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• 제34권3호
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• pp.442-452
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• 2016

Plasmodiophora brassicae Woron.에 의한 뿌리혹병은 배추과 작물에 발생하여 전세계적으로 심각한 피해를 주고 있다. 본 연구에서는 뿌리혹병균에 대한 양배추의 저항성 특성을 조사하기 위하여, 일반 품종, 뿌리혹병 저항성(CR) 품종 및 CR 계통 그리고 저항성과 감수성 계통의 교배로부터 얻은 $F_3$ 계통에서 뿌리혹병균 균주들에 의한 뿌리혹병 발생 조사하였다. 국내 시판 중인 일반 양배추 16품종에서 뿌리혹병균 4균주(DJ, HN1, GN1 및 YC)에 의한 뿌리혹병 발생을 조사한 결과, 이들 뿌리혹병균들은 감수성 배추에서는 유사한 뿌리혹병 발생을 보였으나, 양배추 16품종에서는 균주에 따라 뿌리혹병 발생에 큰 차이가 있었다. 양배추에서 병원력이 가장 강한 균주는 YC 균주였고, 그 다음은 GN1, HN1, DJ 균주순이었다. 일반 양배추 품종에서 뿌리혹병 발생이 뿌리혹병균의 병원력에 따라 차이를 보이는 것과 달리, CR 양배추 계통인 'YCR478'과 CR 양배추 2품종은 YC, GN1, HN1 및 DJ 균주를 포함한 뿌리혹병균 12개 균주에 대하여 모두 고도의 저항성을 보였다. 한편, 'YCR478'과 감수성 계통 'C1176'의 교배로부터 얻은 $F_3$ 107계통의 뿌리혹병균 3개 균주에 대한 저항성 차이를 조사한 결과, YC, GN1 및 DJ 균주에 대하여 감수성 반응을 나타내는 $F_3$ 계통은 각각 89, 33, 6개였다. 이와 반대로 이들 균주에 대하여 저항성을 보이는 계통은 각각 6, 36, 67개였다. 이상의 결과로 양배추의 뿌리혹병 저항성은 P. brassicae 균주들의 병원력 정도에 따라 영향을 받는 것으로 생각되었다.

• 대한한의학회지
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• 제16권1호통권29호
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• pp.184-197
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• 1995

After 45 patients examined as to the result of medical treatment among the ones who came to Dept. of Pain Clinic, Oriental Medical Hospital, Chunju Woosuk University complaining low back pain chiefly from Dec/19/1994 to Feb/7/1995 for 50 days or so were observed clinically, the results were obtained as follows: 1. In duration of the case history, acute stage(37.8%) was the most predominant, and subacute stage(35.6%) and chronic stage(26.7%) were revealed in turn. 2. In opinions of radiation, Spondylosis(26.7%) was the most predominant, Scoliosis(15.6%) and HIVD(11.1%) were revealed in order, and 3 cases were revealed to be negative. 3. In the radiating pain of the lower limb, the radiating pains of the left lower limb were the most predominant and those of the right lower limb and those of both lower limbs were revealed in turn. By the way, 31.1% of patients didn't suffer from the radiating pain of the lower limb. 4. In the grade of the seriousness of subsective symptom, Grade 2(66.7%) was the most predominant, and Grade 3, Grade 4, and Grade 1 followed it in order. 5. In the period of the treatments of medi-acupuncture, 2-5 day treatments(31.1%) were major and 6-10 day treatments(26.7%), 16-20 day treatments(11.1%), 26-30 day treatments(11.1%), over 31 day treatments(11.1%), 11-15 day treatments(4.4%), and 21-25 day treatments(4.4%) followed it by turns. Thus 2-10 day treatments are 57.8% and under 30 day treatments are 57.8% of all. 6. In the frequency of use of each medi-acupuncture, V was most frequency used in 41 cases(91.1%), and 11 cases of HN(24.4%), 8 cases of MOK(17.8%), 4 cases of OK(8.9%), 2 cases of B(4.4%), and a case of I(2.2%) were revealed in turn. 7. In the effect of treatments, 10 cases(22.2%) were excellent, 25 cases(55.6%) were good, 5 cases(11.1%) were fair, and 5 cases(11.1%) were poor. As the result, 88.9% of all changed for the better and all of these were improved within the third trial. 8. The effect of the treatments per durations was 100% in acute stage, 93.8% in subacute stage, and 66.7% in chronic stage. According to that, it was reavealed that the rate of treatments decreased as it came near to the chronic stage. 9. The effect of treatments per radiating pains was 87.5% in the radiating pains of the left lower limb, 81.8% in those of the right lower limb, 100% in those of both lower limbs, and 92.9% in case that patients have no radiating pains. So in the rate of treatments on radiating pains of the lower limbs, the case of both sides or no radiating pains was higher than that of one side. 10. In the effect of treatments per the condition of patients, Grade 4 showed 100% of improvement, Grade 3 showed 90.9% of improvement, Grade 2 showed 86.7% of improvement, and Grade 1 showed 100% of improvement. 11. In the effect of treatments per contents of treatments, the group treated with medi-acupuncture therapy, herb medication therapy, acupuncture therapy, and physiothrapy at the same time(Group 1) showed 100% of improvement, the group treated with medi-acupuncture therapy, acupuncture therapy, and physiotherapy simultaneously(Group 2) showed 73.7% of improvement, and the group treated with medi-acupuncture therapy and acupuncture therapy at the same time(Group 3) showed 100% of improvement. 12. 2-5 day treatments showed 78.6% of improvement, 6-10 day treatments showed 91.7% of improvement, and over 31 day treatments showed 100% of improvement. As the result, genarally the longer the period of treatment was, the better the effect of treatment was. 13. When only V was used, the rate of treatment was 96.2%. When only HN was used, the rate of treatment was 100%. When only MOK was used, the rate of treatment was 100%. When V and HN were used at the same time, the rate of treatment was 33.3%. When V and MOK were used at the same time, the rate of treatment was 100%. When V and OK were used at the same time, the rate of treatment was 100%. When V, HN, and MOK were used at the same time, the rate of treatment was 100%. When V, OK, and MOK were used at the same time, the rate of treatment was 100%. When V, MOK, and B were used at the same time, the rate of treatment was 100%. When V, HN, OK, and I were used at the same time, the rate of treatment was 100%. When V, HN, and B were used at the same time, the rate of treatment was 100%.

• 대한한방내과학회지
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• 제32권1호
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• pp.33-42
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• 2011

Background : Despite recent advances in cancer management, prognosis of ovarian cancer is poor. Anticancer effects of herbal medicine, such as Euonymus alatus Sieb, Oldenlandia diffusa (Willd.) Roxburgh, and Orostachys japonicus A. Berger, have been reported in treatment of ovarian and cervical cancers, but the systematic approaches to explain their molecular mechanism(s) have not yet been established. Objectives : To establish a basis of understanding for anti-cancer mechanisms of herbal medicine, we profiled protein expression in human ovarian and cervical cancer cells treated with the extracts from Euonymus alatus Sieb, Oldenlandia diffusa (Willd.) Roxburgh and Orostachys japonicus A. Berger. Methods : Human ovarian cancer cell line NIH:OVCAR-3, and human cervical cancer cell line HeLa were employed in the present study. Whole protein was obtained from the cells harvested at 48 hours after the treatment with herbal water-extract, and analyzed by 2DE-based proteomic approach. Results : Various changes of protein expression induced by the herbal treatment were monitored : down-regulation of molecular chaperone (calreticulin variant), glycolytic enzymes (D-3-phosphoglycerate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase and alpha-enolase), RNA processing molecules (hnRNP A2/B1), and antioxidant protein (peroxiredoxin 1). Conclusions : Repression of glycolysis has been accepted as the mechanism to increase anticancer reagent's effect. Thus, down-regulation of glycolytic enzymes by the herbal extracts suggested a possible synergistic effect of herbs in the presence of platinum-based therapeutics. In further study, as well as the synergistic effect of the herbs, it has to be further validated whether artificial regulation of hnRNP A2/B1 in ovarian cancer cells affects various cancer survival factors, since RNA processing can be interrupted by deranged expression of hnRNP subtypes, and it results in an inhibition of cancer cell growth.

• The Korean Journal of Physiology and Pharmacology
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• 제20권1호
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• pp.25-33
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• 2016

Ion channels in carcinoma and their roles in cell proliferation are drawing attention. Intracellular $Ca^{2+}$ ($[Ca^{2+}]_i$)-dependent signaling affects the fate of cancer cells. Here we investigate the role of $Ca^{2+}$-activated $K^+$ channel (SK4) in head and neck squamous cell carcinoma cells (HNSCCs) of dif-ferent cell lines; SNU-1076, OSC-19 and HN5. Treatment with $1{\mu}M$ ionomycin induced cell death in all the three cell lines. Whole-cell patch clamp study suggested common expressions of $Ca^{2+}$-activated $Cl^-$ channels (Ano-1) and $Ca^{2+}$-activated nonselective cation channels (CAN). 1-EBIO, an activator of SK4, induced outward $K^+$ current (ISK4) in SNU-1076 and OSC-19. In HN5, ISK4 was not observed or negligible. The 1-EBIO-induced current was abolished by TRAM-34, a selective SK4 blocker. Interestingly, the ionomycin-induced cell death was effectively prevented by 1-EBIO in SNU-1076 and OSC-19, and the rescue effect was annihilated by combined TRAM-34. Con-sistent with the lower level of ISK4, the rescue by 1-EBIO was least effective in HN5. The results newly demonstrate the role of SK4 in the fate of HNSCCs under the $Ca^{2+}$ overloaded condition. Pharmacological modulation of SK4 might provide an intriguing novel tool for the anti-cancer strategy in HNSCC.