• Journal of the Korean Electrochemical Society
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• v.7 no.1
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• pp.26-31
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• 2004

The direct electrochemical studies of four laccases (plant and fungal laccases) have been investigated on a gold electrode functionalized with a new tether of 2.2'-dithiosalicylic aldehyde. Results from these studies indicate that the redox potential of the active site of plant laccase from Rhus vernificera is shifted to a more negative value(255 mV versus SCE) than that of fungal laccase from Pyricularia oryzae (480 mV versus SCE). Mechanistic studies indicate that the reduction of type-1 Cu precedes the reduction of type-2 and type-3 Cu ions when the electrode is poised initially at different potentials. Also a new tether, 2.2'-dithiosalicylic aldehyde, has been used to study the redox properties of two laccases (LCCI and Lccla) covalently attached to a gold electrode. An irreversible peak at 0.47V vs. SCE is observed in the cyclic voltammorams of LCCI. In contrast, the cyclic voltammograms of LCCIa contain a quasi-reversible peak at 0.18V vs. SCE and an irreversible peak at 0.50V vs. SCE. We find that the replacement of the eleven amino acids a the C-terminus with a single cysteine residue $(i.e., \;LCCI{\rightarrow}LCCIa)$ influences the rate of heterogeneous electron transfer between an electrode and the copper containing active sites $(K_{het}\;for\;LCCI=1.0\times10^{-2}\;s^{-1}\;and\;K_{het}\;for\;LCCI_a= 1.0\;times10^{-1}\;s^{-1}\'at\;0.18V\;versus\;SCE\;and\;4.0\times10^{-2}\;s^{-1}\;at\;0.50V\; versus\;SCE)$. These results show for the first time that the change of the primary structure of a protein via site-directed mutagenesis influences both the redox potentials of the copper ions in the active site and the rate of heterogeneous electron transfer.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.5
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• pp.2207-2212
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• 2012

Overexpression of DNA methyltransferase 1 (DNMT1) has been detected in many cancers. Tobacco exposure is known to induce genetic and epigenetic changes in the pathogenesis of malignancy. 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is an important carcinogen present in tobacco smoke; however the detailed molecular mechanism of how NNK induces esophageal carcinogenesis is still unclear. We found that DNMT1 was overexpressed in ESCC tissues compared with paired non-cancerous tissues, the overexpression being correlated with smoking status and low expression of $RAR{\beta}$. The latter could be upregulated by NNK treatment in Het-1A cells, and the increased DNMT1 expression level reflected promoter hypermethylation and downregulation of retinoic acid receptor ${\beta}$($RAR{\beta}$). RNA interference mediated knockdown of DNMT1 resulted in promoter demethylation and upregulation of $RAR{\beta}$ in KYSE30 and TE-1 cells. 3-(4,5-Dimethyl-thiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometric analysis demonstrated that NNK treatment in Het-1A cells could enhance cell proliferation and inhibit cell apoptosis in a dose-dependent manner. In conclusion, DNMT1 overexpression is correlated with smoking status and low expression of $RAR{\beta}$ in esophageal SCC patients. NNK could induce $RAR{\beta}$ promoter hypermethylation through upregulation of DNMT1 in esophageal squamous epithelial cells, finally leading to enhancement of cell proliferation and inhibition of apoptosis.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.9
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• pp.14-19
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• 2019

In this study, the possibility of using marine life for cosmetic materials was assessed by establishing a fermentation process of seaweed, such as Ecklonia cava, Enteromorpha prolifera, Chondria crassicaulis, Eiseniabicyclis, Codium fragile, Seaweed furcata, Gloiopeltis tenax, Grateloupia elliptica, Undaria pinnatifida, and Saccharina japonica. Lactobacillus sakei isolated from Kimchi was used for effective fermentation and whole milk powder was used as an additive. 2.0 % of crushed seaweed and 1.0 % of whole milk powder were added and afterwards, 1.0 % Lactobacillus sakei was added after cooling to $40^{\circ}C$. After cooling and filtering the fermented product, butylene glycol, glycerine, and 1,2-hexandiol, which have the effect of a preservative, were added to mix and complete the final product. Among the ten kinds of seaweeds, the process was found to be highly effective in the fermentation of Ecklonia cava, Codium fragile, Undaria pinnatifida, and Saccharina japonica. The amount of fermentable substances in cosmetics was determined and the safety of the raw material was verified using the HET-CAM (The Hen's egg test-Chorioallantoic membrane) test.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.4
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• pp.341-352
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• 2021

Acerola is an excellent ingredient because of its high natural vitamin C content, but it is difficult to stabilize and has hardly been studied as a cosmetic material. Therefore, this study developed a mixed liposome preparation for stabilizing acerola extract. As a safety test, the skin irritation test was evaluated by BCOP assay and HET-CAM assay. We evaluated the inhibition of tyrosinase activity, the whitening effect of melanin production, and the wrinkle effect of prochloragentype-I C-peptide production, and confirmed the possibility of functional cosmetics. In addition, a cream of liposomes containing acerola extract mixture was developed to evaluate the clinical studies of skin wrinkles and whitening. BCOP assay, HET-CAM assay and human skin primary irritation test results of liposomes containing acerola extract mixture showed no irritation and were safe from skin and eye. The result of tyrosinase activity by 75.8% at 1,000 ㎍/mL. As a result of the melanogenesis inhibition test, liposome with acerola extract showed the melanin content by 46.2% at 1,000 ㎍/mL that does not effect the viability of the B16F10 cell line. The result of collagen production test using ELISA kit, liposomes containing acerola extract mixture showed collagen synthesis ability by 152.1% at 1,000 ㎍/mL that does not affect the viability of the HS68 cell line. But it did not showed any inhibition of collagenase (MMP-1) activity at all concentrations in the MMP-1 activity inhibition test in the HS68 cell line. We performed clinical studies for the whitening and skin-wrinkle activity of cream containing acerola extract mixes liposome, was showed that the melanin contents and wrinkle was statistically significant reduction. These results suggest that liposomes containing acerola extract mixture have safe natural material, and skin wrinkle, whitening effects allowing their application in cosmetics as a natural product.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.37 no.1B
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• pp.31-38
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• 2012

In this paper, we propose a method to improve cell edge users' performance in HetNet (heterogeneous network) systems by cooperatively transmitting signals using remote radio heads (RRHs) located near coverage boundaries, referred to as edge RRHs. The proposed method locates the edge RRHs in specific locations of the cell boundary and provides an efficient operational strategy by adjusting the duty cycle of the edge RRHs and base stations. The effectiveness of the method is demonstrated by experimental performance based on the system model parameters of the CoMP (coordinated multi-point transmission and reception) scenario, which is discussed in LTE-Advanced (Long Term Evolution - Advanced) standard contributions. When compared with conventional methods, utilization of edge RRHs is especially advantageous for the performance improvement of lower percentile users in terms of average throughput and effectively improves the fairness among users.

• Resources Recycling
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• v.7 no.1
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• pp.27-33
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• 1998

A study on thc recovcry oi nickel from waste pickling solutions removed li-ee nil~ica cid and hydmflnoric acid is carried out with pulsed column extractor for thc industrial application. The ~esults show that thc Iron and chromium arc efteclively elunmatcd from the waste solution by precipitalion as the form of hydraxidc with thc adjustment of pH with CaCO;, whlle the nickel is not prcc~pitatcd. Thc cxlraction eficicncy ol nickel with column cxtraclor generally improves 8s the pulse velosity (product of amplmde and frequency) incrcascs, optimum pcrfarmancc typically occuring slightly below an amplihldefrecluency product which results in flooding the column because of excessive emulsiIicalian And the nickel loaded in the organic is erfeclively conce~~trtratebdy ZM H2S0,, ~ I It he stlipping stage. The solubility of NiSO, in the H,SO, solution dccreaaes w~th thc higher H,SO, concentralion and appears to be 55 grL in the 2M HSO* solulian.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2010.06a
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• pp.366-366
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• 2010

Simulation Program (AFORS-HET 2.4.1) was used, include the basic structure of crystalline silicon thin film as above, below Intrinsic a-Si:H films bonded symmetrical structure (Symmetrical structure) were used. Efficiency with variation of the concentration was grown by the a-Si p-type with increasing concentrations of Na, efficiency with increasing a-Si n-type of Nd Concentrations was not changed, was decreased rapidly when concentrations were decreased. Efficiency was increased when c-Si n-type of Nd concentration was increased, otherwise efficiency was decreased when concentration was decreased.

• The Korean Journal of Ceramics
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• v.5 no.1
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• pp.73-77
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• 1999

Capsule free hot isostatic pressing (HIPing) of 12 mol% $CeO_2-88 mo% ZrO_2 (12CeO_2-88ZrO_2)$ powder was conducted at 1100~$1200^{\circ}C$ using the powder crystallized in supercritical methanol followed by supercritical drying. Porous $12CeO_2-88ZrO_2$ ceramics with ~35% open porosity, micropore diameter of ~23 nm and a narrow pore size distribution were fabricated by capsule free hot isostatic pressing at $1100^{\circ}C$. The porosity increased with decrease in HIPing temperature and was accompanied by a steady decrease in fracture strength.

• Electronics and Telecommunications Trends
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• v.27 no.1
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• pp.122-135
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• 2012

최근 스마트폰의 급격한 보급과 음성 위주의 이동통신 서비스가 무선 멀티미디어 콘텐츠를 중심으로 하는 데이터 위주의 서비스로 전환됨에 따라 모바일 데이터가 급증하고 있다. 그러나, LTE에서의 Macro-BS(eNB) 기반의 point-to-point 링크의 스펙트럼 효율은 이미 이론적 한계치에 근접하여 저전력의 소규모 기지국 노드를 채용하는 이종망 환경을 도입하는 노력이 3GPP를 중심으로 전개되고 있다. 본고에서는 급증하는 모바일 트래픽 수요를 수용하기 위해 개발되고 있는 소규모 저전력 셀 기반의 HetNet 기술개발 동향과 대용량의 모바일 콘텐츠를 이동통신 단말기의 근접성을 기반으로 기지국 트래픽 부하 없이 제공하기 위해 개발되고 있는 단말기 간 직접 통신 기술 개발현황을 소개한다.

• ETRI Journal
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• v.40 no.1
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• pp.51-60
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• 2018

In Long Term Evolution (LTE) cellular networks, the transmit power control (TPC) mechanism consists of two parts: the open loop (OL) and closed loop. Most cellular networks consider OL/TPC because of its simple implementation and low operation cost. The analysis of OL/TPC parameters is essential for efficient resource management from the cellular operator's viewpoint. In this work, the impact of the OL/TPC parameters is investigated for homogeneous small cells and heterogeneous small-cell/macrocell network environments. A mathematical model is derived to compute the transmit power at the user equipment, the received power at the eNodeB, the interference in the network, and the received signal-to-interference ratio. Using the analytical platform, the effects of the OL/TPC parameters on the system performance in LTE networks are investigated. Numerical results show that, in order to achieve the best performance, it is appropriate to choose ${\alpha}_{small}=1$ and $P_{o-small}=-100dBm$ in a homogenous small-cell network. Further, the selections of ${\alpha}_{small}=1$ and $P_{o-small}=-100dBm$ in the small cells and ${\alpha}_{macro}=0.8$ and $P_{o-macro}=-100dBm$ in the macrocells seem to be suitable for heterogeneous network deployment.