• 대한두경부종양학회지
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• 제29권1호
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• pp.1-10
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• 2013

연구배경 및 목적 표피성장인자수용체(EGFR)는 HER2/neu(erbB2), HER3(erbB3), HER4(erbB4)를 포함하는 receptor tyrosine ki-nase의 erbB 그룹에 속하는 수용체이다. 표피성장인자수용체의 과발현은 다양한 종류의 암, 특히 두경부편평세포암에서 예후를 악화시킨다고 알려져 있다. 이에 저자들은 하인두편평세포암에서 표피성장인자수용체의 발현 및 분포를 확인하고, 하인두암에서 표피성장인자(EGF)가 암세포의 증식과 침습에 미치는 영향에 대해 알아보고자 하였다. 대상 및 방법 57명의 하인두편평세포암 환자의 조직에서 표피성장인자수용체의 발현을 면역화학적염색을 통해 확인하고, 이에 대해 임상병리학적 요인과 생존율에 대한 분석을 시행하고, 일부 환자의 정상 및 암조직에서 Western blot을 시행하였다. 하인두편평세포암 세포주인 FaDu에서 proliferative assay, colony dispersion, wound healing assay, invasion assay를 시행하여, 하인두암의 진행에서 표피성장인자의 역할에 대하여 분석하였다. 또한 RT-PCR과 Zymography를 통하여 Matrix metalloproteinase(MMP)-2, 9의 발현을 확인하였다. 결 과 63.2%의 하인두편평세포암 조직에서 표피성장인자수용체의 발현이 확인되었다. 표피성장인자수용체의 발현은 정상조직에서 비하여 하인두암 조직에서 유의하게 증가되어 있었으며, 병리학적 병기(p=0.022)가 올라갈수록 유의하게 증가하였으나, 증례수의 제한으로 생존율에서는 통계적 유의성을 얻지는 못했다(p=0.053). in vitro의 결과로 표피성장인자를 FaDu 세포주에 처리하였을 때, FaDu 세포주의 증식이 유의하게 증가되었으며(p<0.05), Transwell invasion chamber상 침습의 증가가 확인되었다(p<0.05). RT-PCR과 zymogram 실험상 표피성장인자처리시 FaDu 세포주의 MMP-2, 9이 발현이 증가되고 활성화되는 것이 확인하였다. 결 론 본 연구에서 표피성장인자수용체의 과발현이 하인두암의 예후 인자로서의 가능성을 확인하였고, 표피성장인자가 하인두편평세포암의 증식과 침습에 관여하는 것을 확인하였다.

• 대한암한의학회지
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• 제24권2호
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• pp.13-21
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• 2019

Objective : The purpose of this study is to report and observe effects of Traditional Korean Medicine (TKM) on stage IV metastatic breast cancer patient. Method : A right breast cancer patient diagnosed with metastatic lesions on liver, lung, spleen, multiple bones and skin on right breast April 2013. The patient received Herceptin + Docetaxel + Zometa from May 2013 and started to receive TKM since July 2013 to decrease side effects of chemotherapy. From December 2013 to November 2014, she had received Herceptin 18 times more. The clinical outcomes were measured by computed tomography, laboratory findings including tumor markers (CEA, CA15-3), liver function test (AST, ALT), and numeric rating scales (NRS). Results : After 6 years of TKM treatment combined with standard chemotherapy, tumor size was partially decreased in lung, liver, right pleura and spleen. Levels of tumor markers also showed decrease. There were no severe adverse events induced by TKM based on National Cancer Institute Common Terminology Criteria for Adverse Event (NCI-CTCAE) version 4.0. Conclusion : This case suggests that TKM combined with standard chemotherapy could be a promising method for treating metastatic stage IV breast cancer.

• Journal of Ginseng Research
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• 제39권2호
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• pp.125-134
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• 2015

Background: Black ginseng (Ginseng Radix nigra, BG) refers to the ginseng steamed for nine times and fine roots (hairy roots) of that is called fine black ginseng (FBG). It is known that the content of saponin of FBG is higher than that of BG. Therefore, in this study, we examined antitumor effects against MCF-7 breast cancer cells to target the FBG extract and its main component, ginsenoside Rg5 (Rg5). Methods: Action mechanism was determined by MTT assay, cell cycle assay and western blot analysis. Results: The results from MTT assay showed that MCF-7 cell proliferation was inhibited by Rg5 treatment for 24, 48 and 72 h in a dose-dependent manner. Rg5 at different concentrations (0, 25, 50 and $100{\mu}M$), induced cell cycle arrest in G0/G1 phase through regulation of cell cycle-related proteins in MCF-7 cells. As shown in the results from western blot analysis, Rg5 increased expression of p53, $p21^{WAF1/CIP1}$ and $p15^{INK4B}$ and decreased expression of Cyclin D1, Cyclin E2 and CDK4. Expression of apoptosiserelated proteins including Bax, PARP and Cytochrome c was also regulated by Rg5. These results indicate that Rg5 stimulated cell apoptosis and cell cycle arrest at G0/G1 phase via regulation of cell cycle-associated proteins in MCF-7 cells. Conclusion: Rg5 promotes breast cancer cell apoptosis in a multi-path manner with higher potency compared to 20(S)-ginsenoside Rg3 (Rg3) in MCF-7 (HER2/ER+) and MDA-MB-453 (HER2+/ER) human breast cancer cell lines, and this suggests that Rg5 might be an effective natural new material in improving breast cancer.

• Journal of Life Science
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• 제10권2호
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• pp.12-13
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• 2000

A human-specific retroposon SINE-R.C2 has been derived from a human endogenous retrovirus HER V-K 10. It is absent in the genome of nonhuman primates and present within the third intron of the human C2 gene that is located in the class III region of the major histocompatibility complex. In the present study, we determined the regional location of the human C2 gene. The analysis of the Genebridge 4 radiation hybrid mapping panel using PCR amplification located the C2 gene between D6S1422 (10.1 cR) and CHLC.GATA4A03 (21.3) with a lod score of>3.0. This allowed us to localize C2 gene on the human chromosome 6 band p21.31.

• 지역사회간호학회지
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• 제8권2호
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• pp.211-224
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• 1997

The purpose of this study is to find a solution to effectively nurse elderly people in the aging society of next generation by analyzing relationship among the social problem of elderly people in the future society, their preferred life style, and their understanding level of an asylum for the aged. Data for this study were collected by questioning 316 persons(257 visitors at C University hospital and 59 residents in Chun - Ra Nam Do) from December 1996 to March 1997. The results were as follows: The assumption that an individual's preferred life style at old age depends on his /her understanding level of the social problem of elderly people was verified ($X^2=9.75$, p<0.00). The assumption that one's understanding of an asylum for the aged depends on his /her understanding level of the social problem of elderly people was verified ($X^2=4.40$, p<0.05). The assumption that one's understanding of an asylum for the aged depends on his/her pre-ferred life style at old age was verified($X^2=34.51$, p<0.01). Among those who want to live together with children when they get old, the following differences in their conception were noted: Elderly people who has lost his /her wife /husband should marry again(p<0.01). Children should take care of their parents(p<0.01). Children should live with their parents(p<0.01), A desirable living style for an elderly people. An undesirable living style for an elderly people. Among those who want to live alone when they get old, the following differences were observed: It is okay for children to live separately from their parents as long as they provide financial support(p<0.01). Any child, regardless of the distinction of sex or the order of birth among siblings, can take care of his/her parents(p<0.05), It is desirable for an elderly people to live alone (p<0.01). It is undesirable for an elderly people to live in an asylum for the aged(p<0.05). It was verified that those who view an asylum for the aged in a negative way believe that children should take care of their parents. Also, it was verified that those who view an asylum for the aged in a positive way believe that children should provide financial support to their parents even though they do not live together(p<0.01). Regardless of the subjects' opinions on seriousness of the social problem of elderly people, the assumption that any child, regardless of the distinction of sex or the order of birth among siblings, can take care of his/her parents was verified(p<0.01) (p<0.05). Regardless of the subjects' view on asylum for the aged, the assumption that it is undesirable place for an elderly people to live was verified (p<0.01). The assumption that one's understanding of an asylum for the aged depends on the distinction of sex among general characteristics such as sex, age, educational background, occupation, and religion was verified(t=-2.82, p<0.01). The assumption that an individual's preferred life style at old age depends on the distinction of sex among general characteristics such as sex, age, educational background, occupation, and religion was verified(t=l.68, p<0.l).

• 수산해양기술연구
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• 제35권3호
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• pp.209-214
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• 1999

The turning circle of a ship is the path followed by her center of gravity in making a turn of 360$^{\circ}$degrees or more with helm at constant angle. But generally it means her path traced at full angle of the rudder. For the ordinary ship the bow will be inside and the stern outside this circle.It has been usually understood that the turning circle is not essentinally affected by ship's speed at Froude numbers less than about 0.30. However, it is recently reported that the speed provide considerable effects upon the turning circle in piloting many ships actually at sea. In this paper, the author analyzed what effects the speed could provide on the turning circle theoretically from the viewpoint of ship motions and examined how the alteration of the speed at Froude no. under 0.30 affect the turning circle actually, through experiments of actual ships of a small and large size.The main results were as follows.1. Even though ship's speed at Froude no. under 0.30, the alteration of the speed affects the turning circle considerably.2. When the full ahead speeds at Froude no. under 0.30 of small and large ships were increased about 3 times slow ahead speeds, the mean rates of increase of the advances, tactical diameters and final diameters of thease ships were about 16%, 21% and 19% respectively.3. When the full ahead speeds at Froued no. under 0.30 of small and large ships were increased about 3 times slow ahead speed, the mean rate of increase of the turning circle elements of large ships was greater 10% than that of small ships. 4. When the full ahead speeds at Froued no. under 0.30 of small and large ships were increased about 3times slow ahead speeds, the mean rates of increase of the tactical diameter and final diameter of thease ships were greater than that of the advances of thease ships. 5. When only alteration of speed or sip's head turning is the effective action to avoid navigational fixed hagards, reducing the speed is always more advantageous than increasing the speed in order to shorten fore or transverse distance.

• 수산해양기술연구
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• 제35권3호
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• pp.210-210
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• 1999

The turning circle of a ship is the path followed by her center of gravity in making a turn of 360$^{\circ}$degrees or more with helm at constant angle. But generally it means her path traced at full angle of the rudder. For the ordinary ship the bow will be inside and the stern outside this circle.It has been usually understood that the turning circle is not essentinally affected by ship's speed at Froude numbers less than about 0.30. However, it is recently reported that the speed provide considerable effects upon the turning circle in piloting many ships actually at sea. In this paper, the author analyzed what effects the speed could provide on the turning circle theoretically from the viewpoint of ship motions and examined how the alteration of the speed at Froude no. under 0.30 affect the turning circle actually, through experiments of actual ships of a small and large size.The main results were as follows.1. Even though ship's speed at Froude no. under 0.30, the alteration of the speed affects the turning circle considerably.2. When the full ahead speeds at Froude no. under 0.30 of small and large ships were increased about 3 times slow ahead speeds, the mean rates of increase of the advances, tactical diameters and final diameters of thease ships were about 16%, 21% and 19% respectively.3. When the full ahead speeds at Froued no. under 0.30 of small and large ships were increased about 3 times slow ahead speed, the mean rate of increase of the turning circle elements of large ships was greater 10% than that of small ships. 4. When the full ahead speeds at Froued no. under 0.30 of small and large ships were increased about 3times slow ahead speeds, the mean rates of increase of the tactical diameter and final diameter of thease ships were greater than that of the advances of thease ships. 5. When only alteration of speed or sip's head turning is the effective action to avoid navigational fixed hagards, reducing the speed is always more advantageous than increasing the speed in order to shorten fore or transverse distance.

• Parasites, Hosts and Diseases
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• 제49권4호
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• pp.409-412
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• 2011

In Korea. many people enjoy eating raw or underkooked freshwater crayfish and crabs which unfortunately may cause paragonimiasis. Here, we describe a case of pulmonary and abdominal paragonimiasis in a 9-year-old girl, who presented with a 1-month history of abdominal pain, especially in the right flank and the right inguinal area, with anorexia. A chest radiograph revealed pleural effusion in both lungs, and her abdominal sonography indicated an inflammatory lesion in the right psoas muscle. Peripheral blood analysis of the patient showed hypereosinophilia (66.0%) and an elevated total serum lgE level (>2,500 IU/ml). The pleural effusion tested by ELISA were also positive for antibodies against paragonimiasis. Her dietary history stated that she had ingested raw freshwater crab, 4 months previously. The diagnosis was pulmonary paragonimiasis accompanied by abdominal muscle involvement. She was improved after 5 cycles of praziquantel treatment and 2 times of pleural effusion drainage. In conclusion, herein, we report a case of pulmonary and abdominal paragonimiasis in a girl who presented with abdominal pain and tenderness in the inguinal area.

• 전기화학회지
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• 제9권1호
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• pp.19-28
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• 2006

The phase-shift method for determining the Langmuir, Frumkin, and Temkin adsorption isotherms ($\theta_H\;vs.\;E$) of H for the cathodic $H_2$ evolution reaction (HER) at a Pt/0.1 M KOH solution interface has been proposed and verified using cyclic voltammetric, differential pulse voltammetric, and electrochemical impedance techniques. At the Pt/0.1 M KOH solution interface, the Langmuir and Temkin adsorption isotherms ($\theta_H\;vs.\;E$), the equilibrium constants ($K_H=2.9X10^{-4}mol^{-1}$ for the Langmuir and $K_H=2.9X10^{-3}\exp(-4.6\theta_H)mol^{-1}$ for the Temkin adsorption isotherm), the interaction parameters (g=0 far the Langmuir and g=4.6 for the Temkin adsorption isotherm), the rate of change of the standard free energy of $\theta_H\;with\;\theta_H$ (r=11.4 kJ $mol^{-1}$ for g=4.6), and the standard free energies (${\Delta}G_{ads}^{\circ}=20.2kJ\;mol^{-1}$ for $k_H=2.9\times10^{-4}mol^{-1}$, i.e., the Langmuir adsorption isotherm, and $16.7<{\Delta}G_\theta^{\circ}<23.6kJ\;mol^{-1}$ for $K_H=2.9\times10^{-3}\exp(-4.6\theta_H)mol^{-1}$ and $0.2<\theta_H<0.8$, i.e., the Temkin adsorption isotherm) of H for the cathodic HER are determined using the phase-shift method. At intermediate values of $\theta_H$, i.e., $0.2<\theta_H<0.8$, the Temkin adsorption isotherm ($\theta_H\;vs.\;E$) corresponding to the Langmuir adsorption isotherm ($\theta_H\;vs.\;E$), and vice versa, is readily determined using the constant conversion factors. The phase-shift method and constant conversion factors are useful and effective for determining the Langmuir, Frumkin, and Temkin adsorption isotherms of intermediates for sequential reactions and related electrode kinetic and thermodynamic data at electrode catalyst interfaces.

• 대한안전경영과학회지
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• 제4권2호
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• pp.103-111
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• 2002

Nowdays cyber education based on the internet is actively developed. But the management of the customers in the cyber education field is not enough. Then, in this paper, we provide the learner with the proposals of lectures to be extremely matched by analyzing the learning capacity and the greatest concern of him(her) using the methods of data mining, such as RFM, prediction, slickness, association rule, classification, and so on.