• Asian Pacific Journal of Cancer Prevention
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• 제15권24호
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• pp.10607-10611
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• 2015

Background: HER2 expression in the primary tumor and its lymph node metastases vary in gastric cancer, reflecting intratumoral heterogeneity. This finding also suggests that proliferation of a different clone in metastatic nodes is possible. In the current study, we aimed to determine the cause of discordance in HER-2 expression in the primary tumor and lymph node metastases for patients with gastric cancer. Materials and Methods: Eighty-one patients with gastric cancer who had undergone radical gastrectomy and were found to have lymph node metastasis upon pathological examination were included. Histopathological samples were obtained from biopsies obtained during patient gastrectomies and lymph node dissection. HER2 status was evaluated by both immunohistochemistry (IHC) and silver in situ hybridization (SISH). Results: Sixty-four (79%) patients were SISH (-), while 17 (21%) were SISH (+) in the primary tumor. However, in metastatic lymph nodes, HER2 status was SISH positive in 5 (28.3%) of the 64 SISH (-) primary tumor specimens. One of the 17 SISH (+) primary tumors was SISH (-) in the metastatic lymph nodes. Thus, SISH results for HER2 in both primary tumors and lymph node metastases were comparable, showing a concordance of 92.5%. In total, six patients demonstrated discordance between the primary tumor and lymph node metastases. The prevalence of HER2 discordance was significantly higher for patients in the pN2 and N3 stages (p=0.007). Although discordant patients had worse survival rates than concordant patients, the differences were not significant (p>0.05).Conclusions: Our study indicates that the frequency of concordance in HER2 status, as determined by IHC or SISH, is high in primary tumors and their corresponding lymph node metastases for patients with gastric cancer. If there is a discrepancy in HER2 status, its evaluation by both IHC and SISH may be useful for detecting patients who would benefit from trastuzumab, and it would therefore help guide decision-making processes in administering treatment.

• 한국콘텐츠학회논문지
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• 제13권10호
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• pp.140-150
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• 2013

희랍 신화속의 조각가 피그말리온은 미적으로 완벽한 조화를 갖춘 조각상을 완성한 후, 자신이 만든 조각상과 사랑에 빠져 비너스에게 그것을 인간으로 변신시켜 달라고 기도한다. 인간으로 변한 조각상과 그는 결혼한다. 버나드 쇼의 영화 <피그말리온>에서 언어학자 히긴스는 런던 길거리에서 꽃 파는 여인 일라이자를 데려와서 그녀의 말투와 태도를 상류층 여성의 그것으로 바꾸어 놓는다. 히긴스는 신화의 피그말리온과 달리 피조물의 겉모습뿐만 아니라 내면까지도 변화시켜 놓아 그녀는 주체성을 회복한 '신여성'으로 변모한다. 영화 <어글리 트루스>의 애비는 철저한 계획성과 지식을 요구하는 방송국 피디로서 성공한 삶을 살고 있는 것 같지만, 그녀의 삶은 진정한 인간에 대한 이해와 애정을 바탕으로 한 이상적 관계를 부정하고 있다. 애비와는 달리 마이크는 본능과 감성을 매우 중요시 여기는 삶을 살고 있으며 마이크의 이러한 태도는 차가운 조각상 같은 애비를 따뜻한 피가 흐르는 인간으로 서서히 바꾸어 놓는다. 두 편의 영화에서 피그말리온들은 결함이 많은 여성을 외적으로 뿐만 아니라 내적으로도 바꾸어 놓아 그들과의 진정한 소통을 가능하게 하고 있다.

• 한국산학기술학회논문지
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• 제20권4호
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• pp.242-249
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• 2019

본 연구의 목적은 부조리극의 대명사라고 할 수 있는 사무엘 베케트의 작품 중에서 여성을 주인공으로 한 유일한 희곡 "행복한 나날들"을 미시적인 관점에서 바라보며 인간의 내면 아이에 초점을 맞추어 등장인물의 상처를 살펴보고, 어떻게 그녀가 상처를 치유해 나가는지를 연구하고자 하는 것이다. "행복한 나날들"에 등장하는 여자 주인공 위니(Winnie)는 그녀의 마음 속 깊은 곳에 자리 잡고 있는 고통과 상처를 인지하지 못하고 힘든 시간을 보낸다. 하지만 자신의 내면 고통을 직면함으로써 그녀는 과거 자신이 가지고 있었던 고통과 마주하게 되고 이를 통해 스스로 치유의 과정을 겪는다. 위니의 삶에 대한 긍정적이고 낙관적인 성격은 그녀의 남편 윌리(Willie)의 행동이 변화할 수 있도록 이끌었으며, 남편 윌리의 행동 변화는 그녀의 상처가 치유될 가능성이 있다는 긍정적인 신호라고 할 수 있다. 따라서 위니는 그녀의 남편 윌리와의 관계를 통해 그녀의 마음속 고통과 상처에 직면하는 기회를 가지게 되었고, 그리고 서서히 자신의 내면 아이의 상처를 치유하는 길을 걷게 되었다고 결론지을 수 있다.

• 한국시조학회지:시조학논총
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• 제42권
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• pp.213-238
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• 2015

한국의 대표적 여류시인으로 평가받고 있는 정운(丁芸) 이영도(李永道)가 작품 활동을 시작한 것은 1946년 5월 "죽순" 창간호에 <제야>를 발표하면서부터다. 일찍부터 유교적 가풍과 전통적 가치관을 몸에 익히며 체화된 그의 한국적 정체성은 자신의 문학에 짙게 표출되어, 1976년 그가 타계할 때까지 30여 년에 걸쳐 한국 여류시조 문단에 독보적인 인물로 남게 된다. 그럼에도 불구하고 이영도는 그의 숭고한 작가 정신과 작품이 보여준 우월성이 그의 개인적인 사생활로 인해 작품 평가의 공정성이 약화된 점 또한 부인하기 어렵다. 이런 관점에서 볼 때 이영도의 시조를 텍스트로 하여 그의 시조가 지닌 문학적 역량과 시조미학을 천착해 볼 필요가 있다. 주지하듯 이영도는 한국의 근 현대 시단에서 굳건하게 자리잡은 시인이다. 그의 시 정신과 시 세계에 대해 고찰한 바를 정리하면 다음과 같다. 첫째, 시대적 격랑기를 거쳐 온 이영도 시조의 원천은 조국이었다. 일제 탄압, 8.15 해방, 민족 분단, 6.25전쟁, 4.19 혁명, 5.16 군사 정변 등 그가 겪은 역사적 파고를 볼 때 의식 있는 지식인으로서 그의 시에 시대정신으로서의 조국애가 반영된 것은 우연이 아님을 알 수 있다. 둘째, 이영도는 시대적 아픔을 남달리 고뇌한 시인이다. 일찍부터 형성된 유교적 가치관과 한국적 정체성은 그가 처한 역사적 현실과 맞물려 자신의 시 세계에 짙게 투영된다. 셋째, 정운 이영도는 그리움의 시인이다. 그것은 이영도의 타고난 풍부한 정서위에 유치환 시인과의 만남으로 정한이 깊어진 것으로 보인다. 넷째, 이영도는 시조의 현대화에 새로운 지평을 연 시인이다. 그의 작품에 내포된 투명한 이미지와 살뜰한 언어의 조탁미는 탁월하다. 요컨대 이영도는 조국애의 시인, 시대적 아픔을 고뇌한 시인, 그리움의 시인으로서 근 현대시조단에 확고하게 자리매김한 철저한 예인으로 평가된다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.4119-4123
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• 2012

Background: Neoadjuvant systemic chemotherapy is the accepted approach for women with locally advanced breast cancer. Anthracycline- and taxane-based regimens have been extensively studied in clinical trials and consequently are widely used. In this study aimed to research the complete response (pCR) rates in different regimens for neoadjuvant setting and determine associated clinical and biological factors. Methods: This study included 63 patients diagnosed with breast carcinoma among 95 patients that had been treated with neoadjuvant chemotherapy between 2007 and 2010. TNM staging system was used for staging. The histologic response to neoadjuvant chemotherapy was characterized as a pCR when there was no evidence of residual invasive tumor in the breast or axillary lymph nodes. Biologic subclassification using estrogen receptor (ER), progesterone receptor (PR), HER2 were performed. Luminal A was defined as ER+, PR+, HER2-; Luminal B tumor was defined as ER+, PR-, HER2-; ER+, PR-, HER2+; ER-, PR+, HER2-; ER+, PR+, HER2+; HER2 like tumor ER-, PR+, HER2+; and triple negative tumor ER, PR, HER2 negative. Results: Patients median age was 54.14 (min-max: 30-75). Thirty-two patients (50.8%) were premenapousal and 31 (49.2%) were postmenapousal. Staging was performed postoperatively based on the pathology report and appropriated imaging modalities The TNM (tumor, lymph node, metastasis) system was used for clinical and pathological staging. Fifty-seven (90.5%) were invasive ductal carcinomas, 6 (9.5%) were other subtypes. Thirty nine (61.9%) were grade II and 24 (38.1%) were grade III. Seven (11.1%) patients were stage II and 56 (88.9) patients were stage III. The patients were classified for ER, PR receptor and HER2 positivity. Seventeen patients had complete response to chemotherapy. Forty patients (63.5%) were treated with dose dense regimen (cyclophosphamide 600 mg/m2 and doxorubicine 60 mg/m every two weeks than paclitaxel 175 mg/m2 every two weeks with filgrastim support) 40 patients (48%) were treated anthracycline and taxane containing regimens. Thirteen patients (76%) from 17 patients with pCR were treated with the dose dense regimen but without statistical significance (p=0.06). pCR was higher in HER2(-), ER(-), grade III, premenopausal patients. Conclusion: pCR rate was higher in the group that treated with dose dense regimen, which should thus be the selected regimen in neoadjuvant setting. Some other factors can predict pCR in Turkish patients, like grade, menopausal status, triple negativity, percentage of ER positivity, and HER2 expression.

• Asian Pacific Journal of Cancer Prevention
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• 제16권14호
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• pp.5773-5777
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• 2015

Background: Oral squamous cell carcinoma (OSCC) is thought to develop from precancerous dysplastic lesions through multistep processes of carcinogenesis involving activation of oncogenes and loss of tumor suppressor genes. The human epidermal growth factor receptor 2 (Her-2/neu [erbB-2]), a cell membrane glycoprotein, is a growth factor receptor that has receptor tyrosine kinase activity. Her2/neu activation plays a central role in cell proliferation and survival. It has been shown that overexpression of Her2/neu increases the rate of cell division and growth, leading to precancerous changes. The aim of the present study was to compare the serum and salivary Her2/neu levels between cases with premalignant and malignant oral lesions. Materials and Methods: Fasting blood samples and unstimulated saliva by passive drooling were collected from three groups of healthy control (n=20), premalignant disorder (PMD) (n=20) and OSCC (n=25) subjects. The HER2 extracellular domain (HER2 ECD) levels were measured using ELISA. Results: The levels of serum Her2/neu showed no significant differences between any of the groups but on the other hand salivary Her2/neu levels were found to be significantly (p<0.05) higher when compared between control (median 68.7 pg/ml, range: 21.5 - 75.8) and OSCC (median 145.6 pg/ml, range: 45.1-191.1). A similar trend was observed when comparing between PMD (median 43.3, range: 22.1 -94.7) and OSCC with a statistical significance of p<0.05. Conclusions: Our study provided evidence of increased salivary Her2/neu in OSCC when compared to PMD and control which was not the case for serum levels. This suggests that probably Her2/neu is not highly amplified as in breast cancer so as to be reflected in serum. Since saliva is in local vicinity of the OSCC, even a mild increase might be mirrored. On the whole, this study proposes Her2/neu as marker for distinguishing premalignant and malignant conditions.

• BMB Reports
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• 제51권12호
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• pp.660-665
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• 2018

Human epidermal growth factor receptor 2 (HER2) inhibitors, such as trastuzumab and lapatinib are used to treat HER2-positive breast and gastric cancers. However, as with other targeted therapies, intrinsic or acquired resistance to HER2 inhibitors presents unresolved therapeutic problems for HER2-positive gastric cancer. The present study describes investigations with AUY922, a heat shock protein 90 (HSP90) inhibitor, in primary lapatinib-resistant (ESO26 and OE33) and lapatinib-sensitive gastric cancer cells (OE19, N87, and SNU-216) harboring HER2 amplification/over-expression. In order to investigate whether AUY922 could overcome intrinsic and acquired resistance to HER2 inhibitors in HER2-positive gastric cancer, we generated lapatinib-resistant gastric cancer cell lines (OE19/LR and N87/LR) by continuous exposure to lapatinib in vitro. We found that activation of HER2 and protein kinase B (AKT) were key factors in inducing intrinsic and acquired lapatinib-resistant gastric cancer cell lines, and that AUY922 effectively suppressed activation of both HER2 and AKT in acquired lapatinib-resistant gastric cancer cell lines. In conclusion, AUY922 showed a synergistic anti-cancer effect with lapatinib and sensitized gastric cancer cells with intrinsic resistance to lapatinib. Dual inhibition of the HSP90 and HER2 signaling pathways could represent a potent therapeutic strategy to treat HER2-positive gastric cancer with intrinsic and acquired resistance to lapatinib.

• Human Ecology Research
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• 제58권2호
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• pp.239-253
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• 2020

This study explored a sandplay therapy case of a woman maturing through conflicts with an adolescent son. The goal of the therapy was to relieve her from conflicts with going through individuation process under a free and protected space in sandplay therapy. Forty-six therapy sessions were held. The client exhibited that she needed a conversation with her feminity and maternity in the initial phase of therapy (1-5, regression). In the intermediate phase of therapy (6-43, struggle), she displayed the scenes of meeting of opposites, shadow, death, nurturing and integration of opposites. In the final phase of therapy (44-46, transformation), she showed the acception of her life with leaving her situations to God. Through sandplay therapy in the free and protected space, this study showed the effectiveness of sandplay therapy since the client achieved individuation by showing the acceptance of her life and could control her enraged feelings.

• 복식문화연구
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• 제10권1호
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• pp.60-75
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• 2002

The purpose of this research is to understand the gender identity expressed in Madonna music video texts and performances. Madonna has reconstructed the fluid identities through the variations of body, images, costumes, and attitudes . The results are as fellows; ① Her punky sexuality is to be seen the flash trash look, kitsch fashion, which reconstructs a good/bad taste, modesty/immodesty, the relations of under/outer wear using bawdy sexuality through her early Virgin tour. ② Her Heterosexuality is to be seen the glamourous look, traditional images of women, which represents the passive feminity of patriarch. ③ Her sadomasochism sexuality is to be seen the bondage look of dominatrix image, which deconstructs sexual taboos; represents sexual power. ④ Her bisexuality is to be seen androgynous look, the 3rd species look using masculinity/feminity signifier, which deconstructs the stereotypes of gender roles. ⑤ Her homosexuality is to be seen the fetish fashion by drag and lesbian, which deconstructs the dichotomy of normality/perversion; opens a possibility of women subjectivity of sexual desires.

• 한국항해학회지
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• 제10권2호
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• pp.1-10
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• 1986

For the purpose of estimation of navigational method and ability about fishing raft, we have practical navigation of her from Chujado on Oct, 7 to Maryang-ri , Kangjin-kun, Jeonranman-do on Oct.10. The results obtained are as follow : 1. Ancient navigation of her is thought to over to an isolated island in the vicinity with a good informations and experiences. 2. Speed of her in outward voyage is 1.34 knots, but is varied with structure and shape of her and ability of fishing raftman. 3. The lee way(U) of her with sail of hight 2.5m and breadth about 1.2m according to windspeed(W) is U=$0.038{\times}W$ 4. We could estimate to take only three days to go over from Chujado to Maryang-ri, Kangjin-kun. Jeonranam-do by her with sail, and make a voyage total 55 miles for thirty-four hours and ten minutes i, e, mean speed is 1.6knots resulty.