• Animal cells and systems
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• 제1권2호
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• pp.345-350
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• 1997

The ${\beta}$-Lactoglobulin (BLG) gene expression is differentially regulated during development of the mammary tissues. Such differential regulation of the BLG gene expression can be reiterated in the cultured mammary HC11 cells. In the growing non-confluent HC11 cells, the BLG promoter activity was shown to be partially repressed by the upstream regulatory sequence. The repression was gradually diminished and switched to activation as the cells grew confluent. The differential regulation of the BLG promoter was controlled by the 5'-regulatory sequence located at the upstream of 205 bp. Electromobility shift assay showed that nuclear extract from HC11 cells differentially bound on the regulatory sequence, depending on the cell confluency, which was in accordance with the differential transcriptional activity. DNase I foot-print assay, however, revealed that all nuclear extracts presented the same foot-prints, regardless of confluency of HC11 cells. These results suggest that differential regulation BLG gene expression by the 5'-regulatory sequence may be accomplished by competitive and/or cooperative binding of differential regulatory factors on the same regulatory element.

• Asian Pacific Journal of Cancer Prevention
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• 제15권2호
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• pp.861-865
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• 2014

Background: High risk HPV (HR-HPV) testing has been recommended as an effective tool along with cytology screening in identification of cervical intraepithelial lesions (CINs) and prevention of their progress towards invasive cervical cancer. The aim of this study was to assess the HR-HPV DNA status by Hybrid Capture 2 (HC2) assay in healthy asymptomatic women of North-East India. Materials and Methods: This study examined cervical cell samples of forty three (n=43) healthy women by HC2 assay. A High Risk HPV DNA kit (Qiagen) was used which can detect 13 high risk HPV types: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68. Results: The mean relative light units (RLU) for samples was in the range of 141-5, 94, 619. HR-HPV DNA was confirmed in 16% (7/43) of participant women samples. Among demographic and clinical parameters, menstrual irregularity (p=0.039) and infection history (p=0.028) has shown statistically significant differences between the HR-HPV-positive and negative groups. In the HR-HPV positive group, two women were confirmed for CINs after colposcopy and histopathologic examination. Conclusions: We suggest that there may be an association between irregular menstruation and infection history of the urogenital tract with HR-HPV DNA prevalence in North-East Indian asymptomatic women. HC2 assay can be a valuable tool for HR-HPV screening.

• Journal of Microbiology and Biotechnology
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• 제8권6호
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• pp.676-684
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• 1998

Baculovirus transfer and expression vectors with Hyphantria cunea nuclear polyhedrosis virus (HcNPV) were constructed. An initial transfer vector, pHcEV, constructed using HcNPV was previously reported (Park et al. 1993. J. Kor. Soc. Viral. 23: 141-151). Herein, the size of the vector was properly reduced, and a functionally perfect vector was constructed and named pHcEV-IV (6.7 kb). The vector has a 2.2-kb HcNPV DNA sequence in the 5'-flanking region of the vector's polyhedrin gene promoter. The 1.8-kb HcNPV DNA sequence, poly A signal sequence, T3 primer sequence, and 13 multicloning site sequences, in order, were ligated in front of the translation start codon of the polyhedrin gene. The cloning indicating marker lacZ gene was inserted into the pHcEV-IV, named pHcEV-IV-lacZ, and transferred into the wild-type virus. Recombinant expression virus, lacZ-HcNPV, was constructed by replacing the lacZ gene in the pHcEV-IV-lacZ with the polyhedrin gene of the wild-type virus. The recombinant virus was isolated from blue plaques that produce $\beta$-galactosidase without polyhedra. The lacZ gene insertion was confirmed by Southern hybridization analysis. The expression of the lacZ gene in Spodoptera frugiperda cells infected with the lacZ-HcNPV was examined by SDS-PAGE and colorimetric assay. One 116-kDa LacZ protein band appeared on the PAGE. The production rate of the $\beta$-galactosidase was approximately 50 international units (IU) per min per ml between 2 to 5 days postinfection (p.i.). The highest activity occurred at five days p.i. was 170 IU/min/$m\ell$. The enzyme activity first appeared about 20 h p.i. as measured by colorimetric assay.

• 대한세포병리학회지
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• 제19권2호
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• pp.119-125
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• 2008

This study was performed to compare the efficacy between a DNA chip method and a Hybrid-Capture II assay (HC-II) for detecting human papillomavirus in patients with intraepithelial lesions of the uterine cervix. From May, 2005, to June, 2006, 192 patients with abnormal colposcopic findings received cervical cytology, HC-II and HPV DNA chip tests, and colposcopic biopsy or conization. We compared the results of HC-II and HPV DNA chip in conjunction with liquid based cervical cytology (LBCC) and confirmed the results of biopsy or conization. The sensitivity of the HPV DNA chip test was higher than HC-II or LBCC. The HPV DNA chip in conjunction with LBCC showed higher sensitivity than any single method and higher sensitivity than HC-II with LBCC. We confirmed that the HPV DNA chip test was more sensitive for detecting HPV in cervical lesions than HC-II, and that it would provide more useful clinical information about HPV type and its multiple infections.

• 대한추나의학회지
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• 제2권1호
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• pp.143-157
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• 2001

Objectives and Methods : To evaluate the mechanism of oxidative damage by xanthine oxydase(XO) and hypoxanthine(HX)-induced oxygen radicals, MTT assay and NR assay were carried out after the cultured mouse spinal sensory neurons were preincubated for 4 hours with various concentrations of XO/HX. And the amount of total protein. neurofilament EIA. lipid peroxidation and LDH activity were measured, to evaluate the protective effect of Herbar Chelidonii(HC) water extract on cultured spinal sensory neurons damaged by XO/HX. after the cultured mouse spinal sensory neurons were preincubated with various concentrations of HC water extract for 3 hours prior to exposure of XO/HX. Results : XO/HX decreased significantly the survival rate of the cultured mouse sensory neurons by NR assay and MTT assay In proportion to concentration and exposed time. In proportion to concentration and exposed time on cultured spinal sensory neurons, XO/HX showed the quantitative decrease of neurofilament by EIA. the decrease of total protein amount by SRB assay and the Increase of lipid peroxidation as well as LDH. HC showed the quantitative increase of neurofilament and total protein, but showed the decrease of lipid peroxidation and LDH activity against the neurotoxicity of XO/HX. Conclusions : From the above results, it is concluded that XO/HX have a neurotoxic effect on cultured spinal sensory neurons and that the herbs extract, such as HC, prevent the toxicity of XO/HX effectively in that they decrease lipid peroxidation and LDH activity.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.104.2-105
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• 2003

Bacterial isolates, CC178, PTC25, HC39 and KY165 originally obtained from the leaves of cucumber or tomato were selected for biocontrol agents against gray mold of cucumber and tomato by in vivo cucumber seedling assay. Each suspension of the selected epiphytic bacteria were sprayed three times at seven-day interval from early stage of cucumber in a field. Incidence of gray mold on cucumber fruits treated with isolates CC178, PTC25, HC39 and KY165 was 15.3％, 18.2％, 23.6％, and 10.4％, respectively, whereas that of control was 38.0％ after 7 days of final spray. On the other hand, treatment with the selected isolates, CC178, PTC25, HC39, and KY165 on tomato showed 2.2％, 1.3％, 2.9％, and 3.5％ in the incidence of gray mold on leaves, whereas that of control was 9.3％. All selected isolates had strong antagonistic activity against Botrytis cinerea on dual culture plate assay.

• 한국식품영양과학회지
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• 제37권10호
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• pp.1244-1250
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• 2008

본 연구는 양파 첨가 청국장 첨가 식이가 고 콜레스테롤 흰 쥐의 지질대사 및 항산화대사에 미치는 영향을 알아보기 위해 Sprague-Dawley 수컷 32마리를 대조군(C), 고 콜레스테롤군(HC), 고콜레스테롤＋양파 첨가 청국장군(HC-OC), 고콜레스테롤＋청국장군(HC-C)로 나누어 실험 식이를 8주간 공급하였다. 8주 후 혈액과 간, 변을 수집하여 혈장, 간 및 변의 지질농도, 혈장 TRAP, CD, 적혈구내 항산화효소활성, comet assay를 통한 DNA 손상을 측정하였다. 모든 군에서 식이섭취량은 유의적 차이가 없었고, HC군에서 체중이 증가하였으며, 그 중 HC-C군에서 체중과 식이효율이 유의적으로 높았다. 간 무게는 C보다 고콜레스테롤 급여 군들에서 유의적으로 증가하였고, 분변량은 고콜레스테롤 식이군들에서 낮았으며, 변의 조지방은 고콜레스테롤 식이 군들에서 증가하였다. 양파 첨가 청국장과 청국장 급여로 HC군보다 간의 총 콜레스테롤 함량은 유의적으로 낮아졌으며, 혈장 지질 저하효과는 나타나지 않았다. 양파 첨가 청국장과 청국장 섭취로 부분적 간 기능 개선 효과를 나타내었으며, 청국장 급여로 혈장 CD농도는 유의적으로 낮아졌다. 그리고 HC-OC군과 HC-C군의 tail intensity는 감소하여 DNA 산화적 손상에 대한 보호 효과를 나타내었다. 따라서 양파첨가 청국장과 청국장은 부분적으로 지질 개선효과와 항산화력이 있음을 확인할 수 있었다. 그러나 양파와 청국장은 각각이 이미 우수한 항산화력을 가지고 있는 기능성식품으로서 이 두 소재의 혼합에 의한 상승효과는 크게 나타나지 않았던 것으로 생각된다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권2호
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• pp.891-894
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• 2014

This study is aimed to investigate the role of paired boxed gene 1 (PAX1) methylation analysis by methylation-sensitive high-resolution melting (MS-HRM) in the detection of high grade lesions in atypical squamous cells cannot exclude high-grade squamous intraepithelial lesion (ASC-H) and compared its performance with the Hybrid Capture 2 (HC2) human papillomavirus (HPV) test. In our study, 130 cases with a diagnosis of ASC-H from the cervical cytological screening by Thinprep cytologic test (TCT) technique were selected for triage. Their cervical scrapings were collected and evaluated by using PAX1 methylation analysis (MS-HRM) and high-risk HPV DNA test (HC2), followed by colposcopy and cervical biopsy. Chi-square test were used to test the differences of PAX1 methylation or HPV infection between groups. In the detection of CIN2+, the sensitivity, specificity, the PPV, NPV and the accuracy of PAX1 MS-HRM assay and high-risk HPV (HR-HPV) tests were respectively 80.6% vs 67.7%, 94.9% vs 54.5%, 83.3%, vs 31.8%, 94.0% vs 84.4%, and 91.5% vs 57.7%. The PAX1 MS-HRM assay proved superior to HR-HPV testing in the detection of high grade lesions (CIN2+) in ASC-H. This approach could screen out the majority of high grade lesion cases of ASC-H, and thus could reduce the referral rate to colposcopy.

• 한국미생물·생명공학회지
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• 제39권3호
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• pp.281-286
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• 2011

발효새우젓의 에탄올 추출물이 지질대사 개선 효능을 알아보기 위하여 흰쥐에 고지방사료를 식이 시키고 추출물의 경구투여 시킨 후, 혈중 지질 관련 물질의 변화를 측정하였다. 혈청 총 콜레스테롤 함량은 고지방 식이를 급여한 HC, HC-SFSL, HC-SFSH, HC-Lov군 모두 7일차, 14일차에 대조군(NC) 보다 유의하게 증가하였다. HC-SFSH군은 7일차에 330 mg/dl, 14일차에는 277 mg/dl로 HC군에 비하여 31.5%, 40.4%의 유의한 저하효과가 관찰되었으며 양성 대조군인 lovastatin보다 감소율 대비 12.6배 높은 혈중 총 콜레스테롤 저하 효과를 나타내었다. 혈중 TG 함량은 7일차에 HC-SFSL군, HC-SFSH군 그리고 HC-Lov군에서 62 mg/dl, 30 mg/dl, 55 mg/dl로 HC군 85 mg/dl에 비해 각각 27%, 64.7%, 35.2%의 유의성 있게 감소하였다. 혈중 LDL-콜레스테롤 함량은 HC-SFSH군에서 고지방만 급여한 HC군 보다 7일차에 22.4%, 14일차에 36.3% 감소 하였으며 양성 대조군인 lovastatin 투여군 보다 혈중 LDL-콜레스테롤 수치를 2.4~3.2배 감소시키는 것으로 나타났다. 고지방 및 새우젓 에탄올 추출물을 14일간 투여 후 간의 조직학적 관찰을 한 결과, 일반식이를 급여한 NC군의 간조직 세포는 간정맥을 중심을 향하여 규칙적 배열을 이루고 있었으며, 간세포의 핵은 중심에 위치하고 세포질은 균질된 구조였다. 반면 고지방 식이만 급여한 HC군의 간 조직은 간조직의 지방입자가 전체적으로 지방구를 형성하며 세포핵이 한쪽으로 치우쳐져 있으며 세포질도 많이 파열되어 전형적인 지방간 형태를 나타내었다. 특히 HC-SFSH군의 간조직은 HC군에 비하여 지방구의 숫자와 세포질의 파열정도가 뚜렷하게 감소된 것이 관찰되었다.

• Clinical and Experimental Reproductive Medicine
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• 제48권3호
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• pp.229-235
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• 2021

Objective: In this study, the effects of Lactobacillus acidophilus on testosterone (TES), follicle-stimulating hormone (FSH), luteinizing hormone (LH), androgen-binding protein (ABP), factor-associated apoptosis (FAS), and total cholesterol (TC), as well as histopathological changes, were investigated in male rats fed a high-cholesterol diet. Methods: The study included three groups. The control (C) group was fed standard-diet for 8 weeks. The hypercholesterolemia (HC) group was fed a 2% cholesterol-diet for 8 weeks. The therapeutic group (HCL) was fed a 2% cholesterol-diet for 8 weeks and administered L. acidophilus for the last 4 weeks. FSH, TES, and FAS levels in testicular tissue were determined using an enzyme-linked immunosorbent assay (ELISA), while another sample was examined histopathologically. LH and ABP levels were determined using ELISA, and serum TC levels were assessed via an autoanalyzer. Results: In the HC group, the TC levels were significantly higher and the LH levels were lower (p<0.05) than in the C group. The ABP levels were lower (p>0.05). In the HCL group, the LH and ABP levels were higher (p>0.05) and the TC level significantly lower (p<0.05) than in the HC group. The TES and FSH levels were lower, and the FAS levels were higher, in the HC than in the C group (p<0.05). In the HCL group, levels of all three resembled control levels. Histologically, in the testicular tissue of the HC group, the cells in the tubular wall exhibited atrophy, vacuolization, and reduced wall structure integrity. However, in the HCL group, these deteriorations were largely reversed. Conclusion: Supplementary dietary administration of an L. acidophilus to hypercholesterolemic male rats positively impacted testicular tissue and male fertility hormone levels.