Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Hybrid Capture 2 Assay Based Evaluation of High-Risk HPV Status in Healthy Women of North-East India

  • Rai, Avdhesh Kumar (DBT Centre for Molecular Biology and Cancer Research, Dr. B. Borooah Cancer Institute) ;
  • Das, Debabrata (DBT Centre for Molecular Biology and Cancer Research, Dr. B. Borooah Cancer Institute) ;
  • Kataki, Amal Chandra (Department of Gynaecologic Oncology, Dr. B. Borooah Cancer Institute) ;
  • Barmon, Debabrata (Department of Gynaecologic Oncology, Dr. B. Borooah Cancer Institute) ;
  • Aggarwal, Dippy (Department of Obstetrics and Gynaecology, Hayat Multi-Speciality Hospital) ;
  • Deka, Pankaj (Department of Gynaecologic Oncology, Dr. B. Borooah Cancer Institute) ;
  • Shrivastava, Sushruta (Department of Gynaecologic Oncology, Dr. B. Borooah Cancer Institute) ;
  • Sharma, Jagannath Dev (Department of Pathology, Dr. B. Borooah Cancer Institute) ;
  • Sarma, Anupam (Department of Pathology, Dr. B. Borooah Cancer Institute) ;
  • Baruah, Upasana (Department of Gynaecologic Oncology, Dr. B. Borooah Cancer Institute) ;
  • Sharma, Mousumi (Department of Pathology, Dr. B. Borooah Cancer Institute)
  • Published : 2014.01.30
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Abstract

Background: High risk HPV (HR-HPV) testing has been recommended as an effective tool along with cytology screening in identification of cervical intraepithelial lesions (CINs) and prevention of their progress towards invasive cervical cancer. The aim of this study was to assess the HR-HPV DNA status by Hybrid Capture 2 (HC2) assay in healthy asymptomatic women of North-East India. Materials and Methods: This study examined cervical cell samples of forty three (n=43) healthy women by HC2 assay. A High Risk HPV DNA kit (Qiagen) was used which can detect 13 high risk HPV types: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68. Results: The mean relative light units (RLU) for samples was in the range of 141-5, 94, 619. HR-HPV DNA was confirmed in 16% (7/43) of participant women samples. Among demographic and clinical parameters, menstrual irregularity (p=0.039) and infection history (p=0.028) has shown statistically significant differences between the HR-HPV-positive and negative groups. In the HR-HPV positive group, two women were confirmed for CINs after colposcopy and histopathologic examination. Conclusions: We suggest that there may be an association between irregular menstruation and infection history of the urogenital tract with HR-HPV DNA prevalence in North-East Indian asymptomatic women. HC2 assay can be a valuable tool for HR-HPV screening.

Keywords

References

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