• Journal of Microbiology and Biotechnology
• /
• v.28 no.1
• /
• pp.40-49
• /
• 2018

We evaluated the antioxidant activity and neuronal cell-protective effect of fucoidan extract from Ecklonia cava (FEC) on hydrogen peroxide ($H_2O_2$)-induced cytotoxicity in PC-12 and MC-IXC cells to assess its protective effect against oxidative stress. Antioxidant activities were examined using the ABTS radical scavenging activity and malondialdehyde-inhibitory effect, and the results showed that FEC had significant antioxidant activity. Intracellular ROS contents and neuronal cell viability were investigated using the DCF-DA assay and MTT reduction assay. FEC also showed remarkable neuronal cell-protective effect compared with vitamin C as a positive control for both $H_2O_2$-treated PC-12 and MC-IXC cells. Based on the neuronal cell-protective effects, mitochondrial function was analyzed in PC-12 cells, and FEC significantly restored mitochondrial damage by increasing the mitochondrial membrane potential (${\Delta}{\Psi}m$) and ATP levels and regulating mitochondrial-mediated proteins (p-AMPK and BAX). Finally, the inhibitory effects against acetylcholinesterase (AChE), which is a critical hydrolyzing enzyme of the neurotransmitter acetylcholine in the cholinergic system, were investigated ($IC_{50}$ value = 1.3 mg/ml) and showed a mixed (competitive and noncompetitive) pattern of inhibition. Our findings suggest that FEC may be used as a potential material for alleviating oxidative stress-induced neuronal damage by regulating mitochondrial function and AChE inhibition.

• The Korean Journal of Food And Nutrition
• /
• v.30 no.6
• /
• pp.1279-1285
• /
• 2017

$Amyloid-{\beta}$ protein ($A{\beta}$) is known to increase free radical production in neuronal cells, leading to cell death by oxidative stress. The purpose of this study was to evaluate the protective effects of $PineXol^{(R)}$ on $A{\beta}_{25-35}$ induced neuronal cell death. Rat pheochromocytoma (PC-12) cells were pre-treated with $100{\mu}g/mL$ of $PineXol^{(R)}$ for 2 h. The cells were exposed to single dose of $30{\mu}M$ $A{\beta}_{25-35}$ for 24 h. Cell death was assessed by a cell count kit-8 (CCK-8) assay, lactate and dehydrogenase (LDH) release assay. An Apoptotic process was analyzed by a protein expression of the Bcl-2 family using western blotting. Cell viability increased in PC-12 cells treated with both $A{\beta}_{25-35}$ and $PineXol^{(R)}$, compared to the control group. $PineXol^{(R)}$ induced a decrease of the Bcl-2 protein expression (p<0.05), while Bax and Sod1 increased (p<0.05), indicating attenuation of $A{\beta}_{25-35}$ induced apoptosis. These results suggest that $PineXol^{(R)}$ may be a good candidate for the prevention of Alzheimer's disease(AD).

• Journal of the Korean Society of Radiology
• /
• v.3 no.2
• /
• pp.23-26
• /
• 2009

This study proposed an improved center array-sequencing phase unwrapping (ICASPU) algorithm. 2% agarose phantom dopped with 0.6mM/l MnCl2 was used with clinical 1.5T MRI system and commercial knee coil. Obtained k-space data(raw-data) was transmitted to PC and reconstructed into phase image with MATLAB software. Previous center array-sequence phase unwrapping algorithm wascompared with proposed ICASP algorithm using second order regression analysis. As a result, we found that the amount of error on proposed ICASPU method is less about 5 times than that of previous CASPU method. In this study, we exploit improved Center array-sequence phase unwrapping algorithm and expect to apply to images including phase informations.

• 제어로봇시스템학회:학술대회논문집
• /
• 2005.06a
• /
• pp.362-367
• /
• 2005

Control network adopted in the national power-plant or manufactory are mostly vendor-dependent products, which have their own special specification, components and individual communication methods. These systems not only raise installation cost, but also bring many difficulties to maintenance and extension. To overcome these problems, this paper introduces a development and application case of a new "PC/Ethernet-based fieldbus" architecture and protocol to be used in mid-level fieldbus. The designed system has a basic idea of "3 Layers fieldbus over Standard-LAN" and implemented on PCs. PC gives user friendly environment. By using an open standard Ethernet in layer 1,2, the system has low cost, wide communication bandwidth and high compatibility. Layer 3 protocol designed for large realtime data communication makes user bypass TCP/IP layer and gives user direct access to the Ethernet. This new protocol eliminates potential collision of Ethernet, and transmits large periodic/non-periodic control data by using long-frame/wide-bandwidth of Ethernet effectively, and offers simple API-Services to the upper layer. Since the system have installed in H-power plant simulator and U-nuclear power plant simulator in Korea, it have been proved to be efficient and stable without any trouble in realtime communication service for full-scope plant simulator that has a lot of control elements.

• Proceedings of the KIEE Conference
• /
• 2005.10b
• /
• pp.319-321
• /
• 2005

한국천문연구원에서는 한국우주전파관측망(Korean VLBI Network; KVN)에서 사용할 관측 시스템 중에서 소규모 자료획득시스템(DAS) 구축을 위하여 기가비트 고속샘플러, PC-VSI 보드, 소프트웨어 상관기를 도입하였다. 고속샘플러는 기가비트급 ADS1000, PC-VSI 보드는 VSI-H(VLBI Standard Interface Hardware) 표준을 따른 PCI 버스를 채택하고 있어 일반 컴퓨터에 설치하여 동작되며, 이 규격을 갖춘 시스템과의 데이터 입출력이 가능하다. 소프트웨어 상관기는 일본 NILT와의 연구협력을 통하여 본 연구원에서도 사용할 수 있게 되었으며, FX와 XF 형 2가지를 모두 갖추고 있다. 하지만 현재 구현된 소프트웨어 상관기는 측지 VLBI 관측을 위해 설계되어 있어, 향후 KVN의 하드웨어 상관기와 함께 천문 VLBI 관측을 위한 3기선 분의 소프트웨어 상관기로 개량하려고 한다. 이상에서 소개한 시스템을 좁은 의미로 K5 시스템이라고도 한다. 본 논문에서는 PC-VSI 보드에 대해 간략히 소개하고, K5 시스템을 이용하여 관측한 결과를 빨리 관측자가 원하는 정보를 얻을 수 있는 소프트웨어 상관기의 구조에 대해서도 소개하고자 한다.

• Structural Engineering and Mechanics
• /
• v.67 no.3
• /
• pp.283-290
• /
• 2018

This paper presents the results of a study that investigated the influence of using recycled coarse aggregate (RCA) and recycled asphalt pavement (RAP) on the properties of pervious concrete (PC). The natural aggregate (NA) was replaced by RCA and RAP in the PC with replacement levels of 0%, 20%, 40%, 60% and 80% by the total weight of NA, respectively. In addition to incorporating RAP and RCA in the same mixes with replacement levels of: (1) 20% RAP and 80% RCA; (2) 60% RAP and 40% RCA; and (3) 80% RAP and 20% RCA. Water permeability, thermal conductivity, density, porosity, void content, and compressive, splitting tensile and flexural strengths were studied in this paper. The results showed that using RCA, RAP, and (RAP-RCA) enhanced the properties of PC in general and improved the mechanical properties significantly in particular. The optimum mix was reported to be the 60% RAP and 40% RCA. Accordingly, the RAP has the potential to be used in PC in order to reduce the negative impact of RAP on the human health and environment.

• Applied Chemistry for Engineering
• /
• v.20 no.2
• /
• pp.154-158
• /
• 2009

Subphthalocyanine (SubPc) derivatives with different kind of substitute groups were synthesized successfully from various precursors using conventional and microwave heating sources. The chemical structure of precursor and product was determined by $^{1}H-NMR$ and FT-IR spectrometer. Also, spectroscopic property was measured by UV-Vis spectrometer. Compared to the conventional synthesis, it was found that SubPc derivatives were synthesized for a shorter reaction time with a higher synthetic yield in the microwave synthesis.

• Journal of Microbiology and Biotechnology
• /
• v.17 no.2
• /
• pp.373-377
• /
• 2007

No phosphatidylcholine (PC) was detected in the membrane of Rhodobacter sphaeroides pmtA mutant (PmtAl) lacking phosphatidylethanolamine (PE) N-methyltransferase, whereas PE in the mutant was increased up to the mole % comparable to the combined level of PE and PC of wild type. Neither the fatty acid composition nor the fluidity of membrane was altered by pmtA mutation. Consistently, aerobic and photoheterotrophic growth of PmtAl were not different from wild type. However, PmtAl showed an extended lag phase (15 h) after the growth transition from aerobic to photoheterotrophic conditions, indicating the PC requirement for the efficient formation of intracytoplasmic membrane (ICM). Interestingly, the B800-850 complex of PmtAl was decreased more than twofold in comparison with wild type, whereas the level of the B875 complex comprising the fixed photosynthetic unit was not changed. Since puc expression is not affected by pmtA mutation, PC appears to be required for the proper formation of the B800-850 complex in the ICM of R. sphaeroides.

• Journal of Microbiology and Biotechnology
• /
• v.12 no.2
• /
• pp.189-195
• /
• 2002

Using Streptomyces sp. YU100 isolated from Korean soil, the fermentative production of phospholipase D was attempted along with its purification and characterization studies. When different carbon and nitrogen sources were supplemented in the culture medium, glucose and yeast extract were found to be the best. By varying the concentration of nutrients and calcium carbonate, the optimal culture medium was determined as 2.0% glucose, 1.5% yeast extract, 0.5% tryptone 0.3% calcium carbonate. During cultivation, the strain secreted most of the phospholipase D in the early stage of growth within 24 h. The phospholipase D produced in the culture broth exhibited hydrolytic activity as well as transphosphatidylation activity on lecithin (phosphatidylcholine). In particular, the culture broth showed 8.7 units/ml of hydrolytic activity when cultivated at $28^{\circ}C$ for 1.5 days. The phospholipase D was purified using 80% ammonium sulfate precipitation and DEAE-Sepharose CL-6B column chromatography, which produced a major band of 57 kDa on a 10% SDS-polyacrylamide gel with purity higher than 80%. The enzyme showed an optimal pH of 7 in hydrolytic reaction, and at pH 4 in a transphosphatidylation reaction. The enzyme activity increased until the reaction temperature was elevated to $60^{\circ}C$. The enzyme was relatively stable at high temperatures and neutral pH, but significantly unstable in the alkaline range. Among the detergents tested as emulsifiers of phospholipids, the highest enzyme activity was observed when 1.5% Triton X-100 was employed. However, no inhibitory effect by metal ions was detected. Under optimized reaction conditions, the purified enzyme not only completely decomposed PC to phosphatidic acid within 1 h, but also exhibited higher than 80% conversion rate of PC to PS by transphosphatidylation within 4 h.

• 한국정보디스플레이학회:학술대회논문집
• /
• 2004.08a
• /
• pp.724-726
• /
• 2004

Highly efficient blue organic light-emitting devices (OLEDs) utilizing the idea of copper phthalocyanine (CuPc)/N,N'-bis-(1-naphthyl)-N,N'-diphenyl,1,1'-biphenyl- 4,4'-diamine (NPB) composite hole transport layer (CPHTL) have been fabricated. The effect of inserting CPHTL upon the performance of blue OLEDs with 2-methyl-9,10-di(2-naphthyl)anthracene (MADN) as the blue emitter has been investigated. Compared with the luminous efficiency of the standard blue device without CPHTL (1.33 cd/A), that of the device with 40:60 CuPc/NPB CPHTL has been increased by more than twice up to 2.96 cd/A with a Commission Internationale d'Eclairage (CIE) coordinates of(x = 0.15, y = 0.10) and a power efficiency of 1.46 lm/W (20 mA/$cm^2$) at 6.39 V. The increased device efficiency is attributed to an improved balance between hole and electron currents arriving at the recombination zone.