• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2005년도 2005 Annual Meeting & International Symposium
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• pp.204-211
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• 2005

The technology of Solid-State-Fermentation (SSF) with corn straw by Pichia ohmeri T021 was studied in this article. After being crushed, the corn straw powder was added into vitriol solutions to hydrolysis, which the quality proportion of corn straw: water: vitriol (98%) is 20:80:1. The mixtures was incubated at 120$^{\circ}C$ for 1 hour, and the hydrolysis rate reached 19%. Following, the mixture was adjusted to pH 4.5 by sodium carbonate and added cellulase (25IFPU/g). The hydrolysis rate reached 15% after the mixture was incubated at 50$^{\circ}C$ for 25h. The mixture which hydrolysed by vitriol was inoculated by Pichia ohmeri T021 (5${\times}10^7$cell/g) and added cellulase (25 IFPU/g) at the same time. The ethanol yield reached 2.99g per 100 gram substrate after the fermenting grains was incubated at 33$^{\circ}C$, pH 4.5 for 5 days.

• 대한기계학회논문집A
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• 제33권4호
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• pp.440-446
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• 2009

This paper was designed to fabricate the miniature spur gear with pitch circle of 2.25mm using extrusion process of a mechanically alloyed Al-78wt%Zn powder. The mechanical alloying of the powder particles were performed for ball milled times of 4h, 8h, 16 and 32h by the planetary ball milling. The mechanical properties of these alloyed powders, which were compacted and sintered-cylindrical preforms, were estimated using compression test. The results showed that the alloyed powder with average particle size of $10{\mu}m$ milled for 32h has the highest compressive(fractured) strength(288MPa). Extrusions of the miniature spur gear using the alloyed powder were carried out at different extrusion temperatures. Extrusion temperature of $300^{\circ}C$ provided the spur gear with the highest relative density and Vickers hardness and without any surface defects.

• 천문학회보
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• 제42권1호
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• pp.33.2-33.2
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• 2017

Symbiotic stars and quasars show strong far UV resonance doublets including O VI 1032 and 1038, which are known to be major coolants of astrophysical plasma with high temperature T > $10^5K$. We investigate the transfer of $H{\alpha}$ and $Ly{\beta}$ in an emission nebula of temperature T ~ $10^5$, where n=2 population is significant. Line photons of $H{\alpha}$ and $Ly{\beta}$ are transferred in the medium through spatial and frequency diffusion altering their identity according to the branching ratios. We adopt a Monte Carlo technique to describe the transfer of $H{\alpha}$ and $Ly{\beta}$ in an emission nebula with a uniform density and a simple geometrical figure. We find that the temperature of the emission nebula is the major controlling parameter to produce a nonnegligible flux of $Ly{\beta}$. In particular, when T exceeds $10^5K$ the number flux ratio may reach ~ 25% with line center optical depth of a few. We discuss the formation of broad $H{\alpha}$ wings from Raman scattering of $Ly{\beta}$ emergent from a hot emission nebula.

• Mycobiology
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• 제33권2호
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• pp.84-89
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• 2005

Five types of agricultural wastes were used for the production of xylanolytic enzyme by Aspergillus flavus K-03. All wastes materials supported high levels of xylanase and ${\beta}-xylosidase$ production. A high level of proteolytic activity was observed in barley and rice bran cultures, while only a weak proteolytic activity was detected in corn cob, barley and rice straw cultures. Maximum production of xylanase was achieved in basal liquid medium containing rice barn as carbon source for 5 days of culture at pH 6.5 and $25^{\circ}C$. The xylanolytic enzyme of A. flavus K-03 showed low thermostability. The times required for 50% reduction of the initial enzyme activity were 90 min at $40^{\circ}C$, 13 min at $50^{\circ}C$, and 3 min at $60^{\circ}C$. Xylanolytic activity showed the highest level at pH $5.5{\sim}10.5$ and more than 70% of the original activity was retained at pH 6.5 and 7.0. The higher stability of xylanolytic enzymes in the broad range of alkaline pH is useful for utilization of the enzymes in industrial process requiring in alkaline conditions. Moreover, the highest production of xylanolytic enzyme was obtained when 0.5% of rice bran was supplied in basal liquid medium. SDS-PAGE analysis revealed a single xylanase band of approximately 28.5 kDa from the culture filtrates.

• Bulletin of the Korean Chemical Society
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• 제33권11호
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• pp.3629-3634
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• 2012

A series of new 4-(pyridin-4-yl)-(3-methoxy-5-methylphenyl)-1H-pyrazoles (6a-k & 7a-l) has been rationally designed based on the structure of the lead compound KIST301080, a selective ROS receptor tyrosine kinase inhibitor, in order to study the activity of ROS of this new class of inhibitors. The compounds were synthesized and screened against ROS kinase, where compound 6h showed moderate inhibitory activity with an $IC_{50}$ value of $6.25{\mu}M$. The study emphasized the importance of the acetonitrile group at the pyrazole ring and also the importance of having a hydrogen bond donor on the distal phenyl ring linked to the pyridine moiety.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.393-397
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• 2003

P. aeruginosa F722는 탄화수소를 분해하는 과정에 biosurfactant (BS)를 생산한다. 탄화수소 분해에 사용되는 C-배지에서 BS 생산량은 0.78 $g/{\ell}$ 이였나, 질소원과 탄소원을 각각 0.05% (w/v) $NH_4Cl\;+\;0.1%$ (w/v) yeast extract과 3.0% (w/v) glucose로 조정한 경우는 BS 생산량이 1.66 $g/{\ell}$로 증가하였다. 최적의 BS 생산조건으로 배양하는 동안 air 1.0 LPM를 공급해 주었을 때는 공기를 공급하지 않을 때의 1.66 $g/{\ell}$보다 약 20% 증가한 1.94 $g/{\ell}$이였다. 뿐만 아니라, glucose 분해속도는 대수증식기와 정지기에서 air를 공급하지 않은 경우0.25, 0.18 $h^{-1}$였으나, 1.0 LPM를 공급한 경우 0.33, 0.29 $h^{-1}$로 조사되었다. 또한, air를 공급하면서 BS 생산 실험을 수행하였을 때, BS 활성이 더 안정적이었다.

• 한국미생물·생명공학회지
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• 제16권2호
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• pp.98-104
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• 1988

미생물 다당류중 viscosifier를 생산하는 세균을 분리하고 그 특성을 조사하였다. 본 세균은 Enterobacter agglomerans로 동정되었으며, 다당류 생산을 위한 조건은 glucose 25 g/ι, peptone 2.0 g/$\ell$, yeast extract 0.5 g/$\ell$, KH$_2$PO$_4$1g/$\ell$, MgSO$_4$.7$H_2O$ 1g/$\ell$, CaCO$_3$2.5g/$\ell$, 3$0^{\circ}C$ pH 5.7이며 이 조건에서의 생산량은 8.41g/L 이었다. 본 다당류의 성분은 갈락토오스(1.0몰), 크실로오스(1.5몰), 글루코 노델타락톤(1.9몰) 및 리보오스(0.03몰)로 되어 있었다. 1% 용액의 점도는 60rpm에서 504mpa.s이며 고유 점도는 45.8 d$\ell$/g 이었다

• 한국식품영양학회지
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• 제25권3호
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• pp.677-684
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• 2012

Poly-${\gamma}$-glutamic Acid(${\gamma}$-PGA)를 다량 생산하는 균주를 우리나라의 전통발효식품인 청국장으로부터 Bacillus subtilis GS-2를 분리하였다. 이 균은 glutamic acid 의존형 균으로, 이 균에 의한 ${\gamma}$-PGA 생산 최적 조건을 검토한 바, 단순배지(L-glutamic acid 2.0%, glucose 1.0%, $NH_4Cl$ 0.5%, $KH_2PO_4$ 0.05%, $MgSO_4$ $7H_2O$ 0.01%, pH 7.0)로 진탕배양(220 rpm) 하였을 때, 배양시간 48시간, 최적온도 $33^{\circ}C$, 그리고 초기 pH 6.5로 나타났다. 영양원으로 glutamic acid 3%, sucrose 3%, $NH_4Cl$ 0.25%, $KH_2PO_4$ 0.15%, $MgSO_4$ $7H_2O$ 0.015%에서 ${\gamma}$-PGA 최대 생산량이 31.0 $g/{\ell}$이었다.

• 한국식품영양과학회지
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• 제33권7호
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• pp.1186-1191
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• 2004

DEAE-sephadex ion exchange column chromatography에 의해 Bacillus sp. 유래 h-mannanase의 정제를 수행하여 비활성은 21.57 units/mg, 정제배율은 93.78배를 나타내었다. 최적 온도는 5$0^{\circ}C$, 최적 pH는 6.0이며, 온도 안정성에서는 30∼5$0^{\circ}C$에서는 90%이상의 잔존활성을 나타내었고,70∼8$0^{\circ}C$에서는 30%이하의 잔존활성을 나타내었다. pH 안정성에서는 pH 5.5∼7.0에서 100%의 잔존활성을 나타낸 반면 pH 2.0∼4.0에서는 40%이하로 감소되 었다. 정제효소에 의해 konjac glucomannan을 가수분해하여 1차 activated carbon column chromatography와 2차 sephadex G-25 gel filtration 에 의해 당가수분해물을 분리 회수하여 TLC및 FACE에 의해 주요 당가수분해물은 중합도 5와 7로 확인되었다. B. iongum, B. btfidum, B. infantis, E. adolescentis, B. animalis, B. breve의 생육활성에 대한 중합도5와7의 영향을 검토하기 위하여 modified-MRS배지상에 탄소원으로 중합도 5와 7을 대체하여 생육활성을 비교한 결과 B. longum의 경우 특징적으로 각각 4.67, 5.33배의 상대활성을 나타내어 우수한 생육활성을 나타내었다. 또한 B. breve에 대해서는 중합도 5 glucomannooligosaccharide를 처리시 2.42배의 생육활성을 나타내었으나 B. infantis와 B. adolescentis에 대해서는 중합도 5와 7의 올리고당을 탄소원으로 대체시 오히려 생육활성이 현저히 감소되었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.77-77
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• 2017

The sensitivities to photoperiod and temperature give guidance to choose an adaptable genotype for specific area in soybean production. However, there is insufficient information about the variation of sensitivities to photoperiod and temperature with wide genetic background. We investigated the sensitivities to photoperiod and temperature using 82 soybean mini core collection lines provided by NIAS gene bank of Japan. The seeds were sown on 28 May and 4 August in 2015, 24 May and 5 August in 2016 at field in Saga, Japan ($33^{\circ}$ 14' 32'' N, $130^{\circ}$ 17' 28'' E) for the early (average photoperiod and temperature: 15.2 h and $25.1^{\circ}C$) and late (13.6 h and $27.2^{\circ}C$) sowing respectively. The plants were also grown in the growth chamber under 12 h photoperiod with three temperature regimes (day/night temperature: $25/18^{\circ}C$, $28/22^{\circ}C$ and $33/28^{\circ}C$). Emergence date, days to first flower were recorded with 10 plants in the field and 2 plants in the growth chamber for each line. The data for daily average temperatures and photoperiodic hours were collected from weather station. The days from emergence to first flower open (DEF) were varied from 23-92 (2015 and 2016) in early sowing whereas 18-68 (2015) and 18-59 (2016) in late sowing. The shortened DEF in late sowing could be caused by both short photoperiod and high temperature in late sowing. However, the accumulated temperatures during emergence to first flower open (ATEF) were less variable in comparison with DEF, suggesting the ATEF is dependent mostly on the photoperiod. The ATEF were found same between early and late sowing in some early flowering lines (e.g. $686.7^{\circ}C$ and $687.6^{\circ}C$ in HEUKDAELIPS, $728.8^{\circ}C$ and $706.3^{\circ}C$ in WILLIAMS'82) which indicated that these would be insensitive to day length. In the growth chamber experiment, the variation in both DEF and ATEF was a little greater at low temperature ($25/18^{\circ}C$) but almost same at middle ($28/22^{\circ}C$) and high ($33/28^{\circ}C$) temperatures. Since the less differences in ATEF were found between the three temperatures, it is suggested that the temperature plays only a quantitative effect on the flower initiation, and the large ATEF in some lines may indicate the stronger photosensitivity even at 12 h or longer juvenile phase. Some lines with the lowest ATEF regardless of growth conditions, such as FISKEBY V, KE 32 (ATEF: 559.6-666.5, 587.7-709.5) might lack the sensitivities to both photoperiod and temperature. The results suggested that soybean genotypes has wider variation in sensitivity to photoperiod, whereas less variation to temperature.