• Journal of Preventive Medicine and Public Health
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• v.50 no.2
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• pp.133-140
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• 2017

Objectives: The purpose of this study was to describe the source, length, number of views, and content of the most widely viewed Zika virus (ZIKV)-related YouTube videos. We hypothesized that ZIKV-related videos uploaded by different sources contained different content. Methods: The 100 most viewed English ZIKV-related videos were manually coded and analyzed statistically. Results: Among the 100 videos, there were 43 consumer-generated videos, 38 Internet-based news videos, 15 TV-based news videos, and 4 professional videos. Internet news sources captured over two-thirds of the total of 8 894 505 views. Compared with consumer-generated videos, Internet-based news videos were more likely to mention the impact of ZIKV on babies (odds ratio [OR], 6.25; 95% confidence interval [CI], 1.64 to 23.76), the number of cases in Latin America (OR, 5.63; 95% CI, 1.47 to 21.52); and ZIKV in Africa (OR, 2.56; 95% CI, 1.04 to 6.31). Compared with consumer-generated videos, TV-based news videos were more likely to express anxiety or fear of catching ZIKV (OR, 6.67; 95% CI, 1.36 to 32.70); to highlight fear of ZIKV among members of the public (OR, 7.45; 95% CI, 1.20 to 46.16); and to discuss avoiding pregnancy (OR, 3.88; 95% CI, 1.13 to 13.25). Conclusions: Public health agencies should establish a larger presence on YouTube to reach more people with evidence-based information about ZIKV.

• The Korean Journal of Food And Nutrition
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• v.31 no.5
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• pp.696-702
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• 2018

This study investigated the quality characteristics of jujube wines produced from various fruits (Campbell Early, aronia, apply). Before the preparation of jujube wine, jujube juice was extracted using hot water ($100^{\circ}C$, 2 hr). Different blends of jujube wines were produced by blending jujube juice with various fruits using Saccharomyces cerevisiae. After 18 days of fermentation, the alcohol content of jujube wines ranged between 11.5~12.7%. The pH of the wine ranged from 3.31 to 3.53 and the total acid content from 0.47 to 0.68%. The hue value of jujube wines ranged from 0.88 to 2.21 and the color intensity ranged from 0.93 to 6.29. The brightness of jujube wines ranged from 32.35 to 92.47, the redness from 0.85 to 59.05, and the yellowness from 17.35 to 51.02. By combining aronia with jujube juice, a significant increase in the total polyphenol content and the antioxidant activity (the DPPH radical scavenging activity) of the wine was observed. The total polyphenol content (401.77 mg%) and the antioxidant activity (76.29%) were the highest in the jujube wine blended with aronia. The total anthocyanin content of jujube wines ranged from 81.66 to 135.98 mg% and the tannic acid content from 42.48 to 316.36 mg%. Based on the results of the present study, we suggest that jujube wine produced by blending jujube juice with aronia had excellent quality as well as quality characteristics of the wine.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.12
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• pp.6343-6348
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• 2012

Objective: The NAD(P)H:quinone oxidoreductase 1 (NQO1) rs1800566 polymorphism, leading to proline-toserine amino-acid and enzyme activity changes, has been implicated in bladder cancer risk, but individually published studies showed inconsistent results. We therefore here conducted a meta-analysis to summarize the possible association. Methods: A systematic literature search up to August 27, 2012 was carried out in PubMed, EMBASE and Wanfang databases, and the references of retrieved articles were screened. Crude odds ratios (ORs) with 95% confidence intervals (CIs) were analyzed for homozygote contrast (TT vs. CC), additive model (T vs. C), dominant model (TT+CT vs. CC), and recessive model (TT vs. CC+CT) to assess the association using fixed- or random-effect models. Results: We identified 12 case-control studies including 3,041 cases and 3,128 controls for the present meta-analysis. Significant association between NQO1 rs1800566 genetic polymorphism and risk of bladder cancer was observed in the additive model (OR = 1.15, 95% CI = 1.01-1.30, p = 0.030). Moreover, in the subgroup analysis stratified by ethnicity, significant associations were observed in Asians (OR = 1.26, 95% CI = 1.08-1.47, p = 0.003 for T vs. C; OR = 1.68, 95% CI = 1.21-2.32, p = 0.002 for TT vs. CC; OR = 1.50, 95% CI = 1.13-1.98, p = 0.005 for TT vs. CT+CC) but not in Caucasians. Conclusions: The results suggest that NQO1 rs1800566 genetic polymorphism may contribute to bladder cancer development, especially in Asians.

• Journal of Fisheries and Marine Sciences Education
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• v.28 no.3
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• pp.746-759
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• 2016

In this study, we monitored the status of marine environment of olive flounder, Paralichythys olivaceus, culture ground in Jeju-do, Republic of Korea. It reported the consumption of raw olive flounder meat containing of Kudoa, Kudoa septempunctata, could induce vomitting and diarrhea in Japan. The Kudoa is a new mycosporean species, researchers found Kudoa from the muscles of olive flounder cultured at western Japan and imported from Jeju-do. We choose two Kudoa positive farms and two negative farms in the mid of concern about the relation between Kudoa and habitat. We found two marine invasive species at the water-pumping seabed at one of the Kudoa positive farm. The concentration of pH and DO at on-growing flounder farms showed the decreasing pattern along side the raceway (influent sea waters > on growing sea waters > outfluent sea waters). The TN and TP values increased gradually following to the raceway (influent sea waters < on growing sea waters < outfluent sea waters). The concentration of COD and SS were in the range of $0.100-2.581mg{\cdot}L^{-1}$, $1.00-12.70mg{\cdot}L^{-1}$, respectively. The calculated residence time was 4hr 32minutes at F1, 11hr 21minutes at F2, and 9hr 50minutes at F3, respectively. It was calculated same distance of 4 km away from effluent pipes. Although direct relation between Kudoa and marine environment could not define well based on this study result, the more studies on marine environmental stressors for olive flounder are required to conduct as a reliable method including socio-economic group and environmental group.

• Journal of the Korean Institute of Landscape Architecture
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• v.32 no.5
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• pp.102-118
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• 2004

The purpose of this study was to propose an ecological management plan by the comprehensive analysis of biotope structures on Namsan Urban Natural Park in Seoul. Classified by actual vegetation, structure of layer and vegetation damage, biotope structures were composed of forest area, compact management area, herb area, cultivated area and non-ecology(urban) area. Succession had seened to stop in the Native forest. Artifical forest was divided into two types. The first, upper layer, was too dense to accommodate lower layer plants, the other case was the appearance of Quercus spp. and the first stage plants of succession following the declination of the upper layer plants. The soil pH of Nam-san Urban Park was 4.21∼4.51, which meant the soil was becoming acid. As the result of acidity, leaching of available nutrition(K/sup +/, NH₄/sup +/, Ca/sup ++/ etc.) was immediately influenced by the natural ecosystem, influence of acid rain was disturbed to becoming organic matter which was use to plants. In the case of a biotope structure management plan, the urban area was prohibited to spread outside. Cultivated and herb area was regenerated to natural forest. In the forest area, the compact management area was maintained with its present condition, and then it is desirable to make a preservation area and to plant shrubs. Planted Pinus densiflora Community was needed to eliminate competitive species of canopy layer, and plant shrubs. Management of deciduous broad-leaved Comm. was maintained in its present conditionand it is desirable to raise the diversity of the understory and shrub layer. The management of the artifical forest seems to be suitable for Q. spp. community. The care of naturalized plants prevents the expansion and restores the structure of wild plants. The soil management was a marked restoration soil ecosystem in order to prevent soil acid and drying.

• Advances in environmental research
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• v.5 no.2
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• pp.109-123
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• 2016

The Dhaka city, the capital of Bangladesh is one of the most densely populated cities in the world. Tongi canal is situated on the north of the city, which connected the Turag river to the west and the Balu river to the east. A total of 26 water samples were collected from the canal to measure irrigation water quality on the basis of their trace metal and major ionic constituents. Trace metals concentrations in water samples were determined using an Atomic Absorption Spectrophotometer. The amount of Fe, Mn, Zn and Pb in water samples ranged from 0.01-0.80, trace-1.02, trace-0.054 and $0.43-0.64{\mu}g\;mL^{-1}$, respectively. The mean concentration of Ca, Mg, Na, K, $HCO_3{^-}$, $Cl^-$, $BO{_3}^{3-}$, $PO{_4}^{3-}$ and $SO{_4}^{2-}$ in water samples were 45.32, 15.33, 151.65, 11.98, 516.06, 94.69, 0.33, 14.02 and $56.21{\mu}g\;mL^{-1}$, respectively. In respect of $HCO_3{^-}$, $SO{_4}^{2-}$, $PO{_4}^{3-}$ and K contents, most of the water samples were found problematic for irrigation. In context of RSC and hardness, 96 and 92% of water samples were graded as unsuitable and hard class, respectively. The study concluded that Pb content in canal water was comparatively high, so it is desirable to take necessary initiative to minimize the contamination level and to monitor its concentration in water routinely.

• BMB Reports
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• v.34 no.6
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• pp.559-565
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• 2001

Protein carboxyl O-methyltransferase (E.C.2.1.1.24) may play a role in the repair of aged protein that is spontaneously incorporated with isoaspartyl residues. The porcine brain carboxyl O-methyltransferase was cloned in the pET32 vector, and overexpressed in E.coh (BL21) that harbors pETPCMT, which encodes 227 amino acids, including tagging proteins at the N-terminus. The protein sequence of the cloned porcine brain PCMT (r-pbPCMT) shares a 98% identity with that of human erythrocyte PCMT and rat brain PCMT. It is 100% identical with that of bovine brain. The r-pbPCMT was purified using Ni-NTA affinity chromatography and digested by enterokinase in order to remove the protein tags. Then Superdex 75HR gel filtration chromatography was performed. The r-pbPCMT exhibited similar in vitro substrate specificities with the PCMT that was purified from porcine brain. The molecular weight of the enzyme was estimated to be 24.5 kDa on the SDS polyacrylamide gel electrophoresis. The $K_m$ value was $1.1{\times}10^{-7}\;M$ for S-adenosyl-L-methionine. S-adnosyl-L-homocysteine was a competitive type of inhibitor with the $K_i$ value of $1.38{\times}10^{-4}\;M$. The enzyme has optimal activity at pH 6.0 and $37^{\circ}C$. These results indicate that the expressed enzyme is functionally similar to the natural protein. It also suggests that it may be a suitable model to further understand the function of the mammalian enzyme.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.6
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• pp.988-1001
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• 1999

The rumen ecosystem is increasingly being recognized as a promising source of superior polysaccharide-degrading enzymes. They contain a wide array of novel enzymes at the levels of specific activities of 1,184, 1,069, 119, 390, 327 and $946{\mu}mol$ Reducing sugar release/min/mg protein for endoglucanase, xylanase, polygalactouronase, amylase, glucanase and arabinase, respectively. These enzymes are mainly located in the surface of rumen microbes. However, glycoside-degrading enzymes (e.g. glucosidase, fucosidase, xylosidase and arabinofuranosidase, etc.) are mainly located in the rumen fluid, when detected enzyme activities according to the ruminal compartments (e.g. enzymes in whole rumen contents, feed-associated enzymes, microbial cell-associated enzymes, and enzymes in the rumen fluid). Ruminal fungi are the primary contributors to high production of novel enzymes; the bacteria and protozoa also have important functions, but less central roles. The enzyme activities of bacteria, protozoa and fungi were detected 32.26, 19.21 and 47.60 mol glucose release/min/mL mediem for cellulose; 42.56, 14.96 and 64.93 mmol xylose release/min/mL medium after 48h incubation, respectively. The polysachharide-degrading enzyme activity of ruminal anaerobic fungi (e.g. Neocallimastix patriciarum and Piromyces communis, etc.) was much higher approximately 3~6 times than that of aerobic fungi (e.g. Tricoderma reesei, T. viridae and Aspergillus oryzae, etc.) used widely in industrial process. Therefore, the rumen ecosystem could be a growing source of novel enzymes having a tremendous potential for industrial applications.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.5
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• pp.471-477
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• 1997

A study detennined whether certain biochemical and physiological variables were altered during marginal Zn deficiency. Ten weaned crossbred Hereford Angus heifer calves, weighing $163{\pm}2kg$, were utilized. Five calves were fed a Zn - deficient (- Zn) brome-alfalfa hay diet containing 17 mg Zn/kg diet DM, and five calves were fed a Zn-adequate (+Zn) diet with 23 mg Zn/kg diet DM from $ZnSO_4$ added to the - Zn diet (total diet, 40 mg Zn/kg diet DM), for 32 d. At 21 d the - Zn calves had a reduction (p < .05) in feed efficiency. By 25 d, plasma Zn and alkaline phosphatase concentrations were reduced (p < .05) in the - Zn calves. Blood urea nitrogen, glucose, insulin, IGF-I, Cu plasma concentration and Zn and Cu concentrations of red blood cell (RBC) and liver were not altered (p > .05) by the - Zn diet through 25 d. In response to a single i. m. injection of dexamethasone (20 mg) on d 25, calves fed the two dietary Zn amounts showed no changes (p > .05) in plasma or RBC Zn and Cu concentrations, serum IGF-I, insulin, and glucose when measured at 6, 12, 24, 48, 72, and 96 h after injection. In response to an intradermal injection of phytohemagglutinin on d 30, cell mediated immune (CMI) response was reduced (p < .05) in the - Zn calves. These observations indicate that during a marginal Zn deficiency in calves, there was a decrease in feed efficiency, plasma Zn, serum alkaline phosphatase, and CMI response.

• BMB Reports
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• v.44 no.1
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• pp.52-57
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• 2011

Termites play an important role in the degradation of dead plant materials and have acquired endogenous and symbiotic cellulose digestion capabilities. The feruloyl esterase enzyme (FAE) gene amplified from the metagenomic DNA of Coptotermes formosanus gut was cloned in the TA cloning vector and subcloned into a pET32a expression vector. The Ft3-7 gene has 84% sequence identity with Clostridium saccharolyticum and shows amino acid sequence identity with predicted xylanase/chitin deacetylase and endo-1,4-beta-xylanase. The sequence analysis reveals that probably Ft3-7 could be a new gene and that its molecular mass was 18.5 kDa. The activity of the recombinant enzyme (Ft3-7) produced in Escherichia coli (E.coli) was 21.4 U with substrate ethyl ferulate and its specific activity was 24.6 U/mg protein. The optimum pH and temperature for enzyme activity were 7.0 and $37^{\circ}C$, respectively. The substrate utilization preferences and sequence similarity of the Ft3-7 place it in the type-D sub-class of FAE.