• Korean Journal of Agricultural Science
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• v.43 no.4
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• pp.641-649
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• 2016

This study was conducted to evaluate the yield of bio-ethanol produced by separate hydrolysis and fermentation (SHF) with the pretreated rapeseed straw (RS) using crude enzyme of Cellulomonas flavigena and Saccharomyces cereviase. Crude enzyme of C. flavigena showed enzymatic activity of 14.02 U/mL for CMC 133.40 U/mL, for xylan 15.21 U/mL, for locust gum and 15.73 U/mL for rapeseed straw at pH 5.0 and $40^{\circ}C$, respectively. The hemicellulose contents of RS was estimated to compromise 36.62% of glucan, 43.20% of XMG (xylan + mannan + galactan), and 2.73% of arabinan by HPLC analysis. The recovering ratio of rapeseed straw were investigated to remain only glucan 75.2% after 1% $H_2SO_4$ pretreatment, glucan 45.44% and XMG 32.13% after NaOH, glucan 44.75% and XMG 5.47% after $NH_4OH$, and glucan 41.29% and XMG 41.04% after hot water. Glucan in the pretreatments of RS was saccharified to glucose of 45.42 - 64.81% by crude enzyme of C. flavigena while XMG was made into to xylose + mannose + galactose of 58.46 - 78.59%. Moreover, about 52.88 - 58.06 % of bio-ethanol were obtained from four kinds of saccharified solutions by SHF using S. cerevisiae. Furthermore, NaOH pretreatment was determined to show the highest mass balance, in which 21.22 g of bio-ethanol was produced from 100 g of RS. Conclusively, the utilization of NaOH pretreatment and crude enzyme of Cellulomonas flavigena was estimated to be the best efficient saccharification process for the production of bio-ethanol with rapeseed straw by SHF.

• Journal of Microbiology and Biotechnology
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• v.27 no.8
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• pp.1500-1512
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• 2017

In this study, the siderophore-producing characteristics and conditions of Bacillus sp. PZ-1 were investigated and the enhancement of siderophores on Pb uptake and translocation in Brassica juncea were determined. Results of single factor experiment showed that glucose, pH, and $Pb(NO_3)_2$ could stimulate PZ-1 growth and siderophore production. The maximum siderophore production of 90.52% siderophore units was obtained by response surface methodology optimization at the glucose concentration of 21.84 g/l, pH 6.18, and $Pb(NO_3)_2$ concentration of $245.04{\mu}mol/l$. The type of siderophore was hydroxamate and its concentration in the fermentation broth amounted to $32.24{\mu}g/ml$. Results of pot experiments indicated that the siderophores enhanced B. juncea to assimilate more Pb from soil with the uptake ratio from 1.04 to 2.74, and to translocate more Pb from underground to overground with the TF values from 1.21 to 1.48. The results revealed that Bacillus sp. PZ-1 could produce abundant siderophores and might be potentially used to augment the phytoextraction of Pb from soil.

• Journal of Environmental Science International
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• v.21 no.9
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• pp.1079-1085
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• 2012

Ground seawater quality was investigated, and the algal growth potential (AGP) tests were performed along the Tongyeong coast to examine the inflow of materials, which is needed for the red tide species Cochlodinium polykrikoides to grow. The study was conducted to determine the mechanism for C. polykrikoidesred tides. Water temperature, salinity, pH, and dissolved oxygen (DO) ranged from 16.05 to $20.74^{\circ}C$, 18.20 to 32.11 psu, 6.00 to 7.61, and 3.41 to 7.91 mg/L (41.4-96.1%), respectively. No seasonal variation was observed in water temperature. The salinity, pH, and DO saturations at most stations were lower than those of coastal seawater at Tongyeong. The ${NH_4}^+$-N, ${NO_{2+3}}^-$-N, ${PO_4}^{3-}$-P concentrations ranged from 0.43 to 16.00 ${\mu}M$, 1.50 to 132.38 ${\mu}M$, and 1.30 to 6.29 ${\mu}M$, respectively; the values at some stations were much higher than observed in Tongyeong coast seawater. Using seawater from station B, C. polykrikoides grew consistently, with a high growth rate, similar to the red tide in nature. This seawater appeared to contain materials needed by C. polykrikoides to grow. Therefore, C. polykrikoides red tides seem to occur wherever the ground sea water contains materials that are needed for its growth.

• Journal of The Korean Astronomical Society
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• v.41 no.5
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• pp.121-137
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• 2008

We present optical and near-infrared imaging and long-slit spectroscopy for the blue compact dwarf galaxy (BCD) Mrk 49 in the Virgo Cluster. The surface brightness distribution analysis shows that Mrk 49 consists of an off-centered blue bright compact core of r = 10" and a red faint outer exponential envelope. The $H_{\alpha}$ image and color difference suggest that these two components have different stellar populations: a high surface brightness population of massive young stars and an underlying low surface brightness population of older stars. The redder near-infrared colors of the inner most region suggest that the near-infrared flux of Mrk 49 originates from evolved massive stars associated with the current star-forming activity. The total apparent magnitude is $B_T\;=\;14.32$ mag and the mean effective surface brightness is ${\mu}_{eff}(B)\;=\;21.56$ mag $arcsec^{-2}$. Long-slit spectroscopy shows that Mrk 49 rotates apparently as a solid body within r = 10" in a plane at position angle 55 degrees with an amplitude of about $20\;km\;sec^{-1}$. The measured radial velocity of Mrk 49 was derived as $1,535\;km\;sec^{-1}$; and the total mass of stars and gases is in the range of 3 to $6\;{\times}\;10^9\;M_{\odot}$. The mass-to-light ratios for the central region of Mrk 49 in I and B band are estimated 1.0 and 0.5, respectively. The upper limit of the dark matter to visible matter ratio seems to be < 5. The oxygen abundance is $12\;+\;\log(O/H)\;=\;8.21\;{\pm}\; 0.1$ which is about one quarter of the solar value while the relative helium abundance appears to be similar to that of the sun.

• Journal of Environmental Health Sciences
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• v.8 no.1
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• pp.1-11
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• 1982

A study on the water pollution of Gumho river by the relationship between physio-chemical conditions and water quality level by phytoplankton was examined at 7 sampling positions during the period from Aug. 1 to Nov. 30, 1981. Examination of physio-chemicat water analysis such as temperature, pH, DO, BOD, and biological analysis are as follows: 1. pH was in the range of 6.6-7.3. 2. At all positions DO was0.5-11.9 mg/l. But at Shinchun bridge and Gangchang was 0.5-3.9 mg/l. 3. BOD was in the range of 3.4-29.2 mg/l. Banyawol, Dongchon and Gumdan was shown good condition. But at Shinchun bridge was 21.1-29.2 mg/l. 4. The plankton identification in this study period showed, Cyanophyceae is 7 genera 13 species, Bacillariophyceae is 11 genera 32 species, and Chlorophceae is 17 genera 27 species: total 35 genera 72 species. 5. In the point of phytoplankton classification, upper stream of Banyawol, Dongchon and Gumdan which BOD was 3.4-8.7 mg/l, dominant phytoplanktons were Synedra ulna, Ulothrix sp., Oscillatoria sp. and Frusturia rhomboides. At Shinchun bridge which BOD was 21.1-29.2 mg/l, Microcystis aeruginosa, Closterium acerosum and Oscillatoria sp were found a small. At 3rd gongdan which BOD was 9.2-12.5 mg/l, dominant species were Synedra ulna, Hormidium sp and Actinastrum hantzschii. At Paldal which BOD was 7.8-9.2 mg/l, dominant species were Nitzschia palea, Synedra ulna and Scenedesmus bijuga. At Gangchang of down stream which BOD was 6.9-9.2 mg/l, dominant phytoplanktons were Closterium acerosum, Microcystis aeruginosa and Actnastrum hantzschii. 6. The results of biological water analysis by saprobic system were as follows: Banyawol was from oligosaprobic to $\beta$-mesosaprobic, Dongchon and Gumdan was from $\beta$-mesosaprobic to $\beta$-mesosaprobic, Shinchun bridge was polysaprobic, 3rd gongdan was from $\alpha$-mesosaprobic to $\beta$-polysaprobic, Paldal was $\beta$-polysaprobic and Gangchang was $\alpha$-polysaprobic.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.1
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• pp.89-99
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• 2016

The effects of retail cut type on chemical, quality and nutritional characteristics of horse meat were studied. Jeju female breed horses (n = 9) at 32-mo-old were slaughtered and the carcasses at 24 h post-mortem were fabricated into 10 retail cuts including: tender-loin, loin, strip-loin, shoulder-chuck-roll, shoulder-clod, top-round, outside-round, brisket, short-plate-brisket, and shank. The results revealed that all of parameters (chemical, meat quality and nutritional composition) examined significantly (p<0.05) differed between the cuts. The chemical composition range (minimum to maximum) of cuts was found as such: moisture 65.06% to 71.69%; protein 19.07% to 21.28%; collagen 1.40% to 2.45%; fat 2.56% to 12.14% and cholesterol 55.76 to 79.50 mg/100 g. Shoulder-chuck-roll had the highest pH and water-holding capacity, while top-round had the highest cooking loss. Shear force ranged between the cuts from $2.80kg/cm^2$ to $4.98kg/cm^2$. The Cu, Fe, and Zn contents ranged between the cuts from 1.52 mg/kg to 2.75 mg/kg, 21.25 mg/kg to 30.85 mg/kg, and 16.51 mg/kg to 40.42 mg/kg, respectively. Additionally, most of the cuts studied showed favorable polyunsaturated fatty acid/saturated fatty acid, n-3/n-6 and essential amino acid/non-essential amino acid ratios.

• Journal of the Korean Chemical Society
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• v.32 no.4
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• pp.377-384
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• 1988

Carotenoprotein from Salmo Salar eggs was purified and characterized by CM-cellulose, 50% $(NH_4)_2SO_4$, DEAE-cellulose and sephadex G-75 column. The chromoprotein had a spectrum with ${\lambda}_{max}$ 409, 540 and 580nm in p-buffer (pH 7.0) at initial step. Molecular weights by sephadex G-200 gel filtration were 50, 200 and 26,000 daltons. SDS-PAGE analysis showed a structure with four identical subunits (12,500 daltons). Its sample retained a small amount of carbohydrates and lipids. Amino acids were analyzed, and mannose, galactose and glucosamine also were identified. Carotenoid extacted with acetone was found to be astaxanthin ester by partition test, epoxy test, iodine test, allylic test, reduction, acetylation, uv/vis, ir and nmr datas. Stearate (47.9%) and palmitate (21.4%) were predominant fatty acids in the astaxanthin ester.

• Journal of Korean Society of Forest Science
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• v.105 no.3
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• pp.309-314
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• 2016

A locally adapted plant population under harsh environmental changes might survive for a long generation through maintaining proper level of genetic diversity. When it happens losing the genetic diversity too much fast, the population could be declining and probably become extinct. An isolated small population of Populus davidiana was investigated to study out the genetic diversity and the fine-scale spatial genetic structure. The estimated number of adult trees in the population of Mt. Worak, South Korea, was 350 in the total area of $14,000m^2$. The number of adults in a study plot ($70m{\times}70m$) was 123. The average age was 16-year-old and a 32-year-old tree was the oldest. The distribution of individuals was slightly aggregated in the plot. Sixty-one among the 123 individuals were randomly sampled to estimate genetic variation using AFLP markers. One hundred fifty-one (77%) of total 196 amplicons were polymorphic from six AFLP primer combinations. The average number of loci per primer combination was 32.7 (S.D.=7.2). Expected heterozygosity ($H_e$) and Shannon's diversity index (S.I.) were 0.154 and 0.254, respectively. These values were extremely lower than those of other P. davidiana populations in South Korea. Genetic patchiness was showed within 21 meters by spatial autocorrelation analysis and the isolated small size of population might be mainly attributed to the formation of such small patch size.

• Korean Journal of Microbiology
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• v.33 no.1
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• pp.15-21
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• 1997

We studied the characteristics of extracellular autolysins from Moraxella sp. CK-1 which has been known to lyse the cyanobacterial cell walls. This bacterium excreted autolysins from the early exponential growth phase. These enzymes showed optimal action condition of 60-$70^{\circ}C$ and pH 9.0. Whereas $Na^{+}$, $K^{+}$ and $Li^{+}$ ions exhibited positive effect on the enzyme activity, $Ba^{2+}$, $Mg^{2+}$, $Ca^{2+}$ and $Mn^{2+}$ ions exhibited negative effect. Especially, $Fe^{2+}$ and $Cu^{2+}$ ions almost completely suppressed the activity. Four extracellular autolysins of 30, 32, 38 and 41 kDa were detected in renaturing SOS-PAGE gel containing 0.2% heat-killed Micrococcus luteus cells as substrate. Among these 4 autolysins, 2 enzymes of 32 and 41 kDa distributed in the culture medium throughout the experimental time, but the 38 kOa enzyme diminished and 30 kOa began to appear at mid-exponential growth phase. When SOS-insoluble peptidoglycan of M. luteus was treated with the autolysins of Moraxella sp. CK-l, the concentration of free amino groups in reaction mixture increased. This indicates that the autolysins are N-acetylmuramyl-L-alanine amidase or endopeptidase.

• Journal of Korean society of Dental Hygiene
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• v.21 no.5
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• pp.517-526
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• 2021

Objectives: This study was conducted to analyze the sugar content, acidity, and viscosity of the Korean frozen desert. Methods: A total of 60 types of frozen desserts marketed in Korea were randomly selected and classified into frozen desserts, milk-fat ice cream group, and non-fat ice cream group according to the classification criteria for frozen desserts. Each product was treated under the same conditions to investigate the component of each product, and sugar content, acidity, and viscosity were measured. Frequency analysis and one-way ANOVA were performed. Results: As a result of analyzing the characteristics related to dental caries activity by group, the frozen dessert had the lowest average pH of 3.67, and the sugar content was significantly higher in the milk-fat group (33.22) and non-fat group (32.89) than in the frozen dessert. The viscosity was also the highest in the milk-fat group at 32.62, and the frozen dessert was significantly lower at 9.42. Conclusions: Due to the abnormal temperature and spread of the coronavirus, consumption of frozen desserts at home is on the rise. To prevent dental caries in children, education is needed for children and their guardians for proper oral care after eating frozen desserts.