• 제목/요약/키워드: H143

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Characterization of Formation of Fructose during Acid Hydrolysis and Enzyme Treatment of Fructose Saccharides (과당 탄수화물의 산가수 및 효소적 분해 특성연구)

  • Kang, Soon-Ah;Lee, Eun-Young;Jung, Sung-Je;Kim, Sang-Moo;Lee, Jae-Cheol;Jang, Ki-Hyo
    • KSBB Journal
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    • v.21 no.2
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    • pp.140-143
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    • 2006
  • Acid- and enzymatic hydrolysis properties of two fructans(inulin and levan) and their oligofructoses has been investigated. At pH 1, the initial fructose release rate differs and is rapidly hydrolyzed in the order of levan oligosaccharide and inulin oligosaccharide, levan, inulin. At pH 4.5, 7 and 14, no or little amount of fructose are found from four samples. At the presence of inulinase in the reaction mixture, the fructose is rapidly produced from all samples, whilst invertase treatments show low activities. The results allow the estimation of the fructose release rate in many foodstuff processing conditions.

Optimum pH Condition of Defatted Rice Protein Extraction by Alkaline Method (알칼리 추출법에 의한 탈지 미강 단백질 추출의 최적 pH 조건)

  • Kim, Won;Jung, So-Young;Hong, Kwang-Won
    • Food Engineering Progress
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    • v.15 no.2
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    • pp.143-147
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    • 2011
  • For efficient extraction of protein from defatted rice bran, the 5 ranges of extraction pH (8, 9, 10, 11 and 12) and the 3 ranges of isoelectric precipitation pH (2, 4 and 6) were used. The protein content, browning reaction, the electrophoresis pattern and the recovery yield of soluble protein at each pH range were compared each other. The recovery yield of soluble protein increased in proportion to extraction pH, but at the same time, browning reaction became more conspicuous. The most amount of protein was recovered at the precipitation pH of 4. The SDS-PAGE patterns of the extracted proteins showed no significant correlations between pH and the protein content, but the highly alkaline condition was more advantageous to extract protein less than 35 kDa. In each pH range, the recovery yield of soluble protein averagely reached 32.5% on the basis of extraction. In result, it was found that combination of extraction pH 10 and precipitation pH 4, which resulted in 37.65% of recovery yield and low level of browning reaction, was the optimum condition for the extraction of protein from defatted rice bran.

Topographical Chemical Properties of Paddy Soils in Gyeongnam Province (경남지역 논토양 지형과 화학성의 관계)

  • Lee, Young-Han;Sonn, Yeon-Kyu;Lee, Seong-Tae;Heo, Jae-Young;Kim, Min-Keun;Kim, Eon-Seok;Song, Won-Doo;Zhang, Yong-Sun;Jeon, Weon-Tai;Ok, Yong-Sik
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.2
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    • pp.143-148
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    • 2012
  • Sustainable agriculture requires reliable information on the spatial distribution of the soil chemical properties for effective management of paddy fields. In order to provide adequate soil nutritional information for environmental-friendly agriculture, we investigated the soil chemical properties for 260 paddy soils sampled in Gyeongnam Province from 1999 to 2011 by four years of interval. Soil chemical properties of pH, organic matter, available phosphate and silicate, exchangeable potassium, calcium, and magnesium contents were analyzed. The pH value, organic matter, available phosphate and silicate, and exchangeable calcium and magnesium in paddy soils were significantly elevated in 2011 compared to 1999. The excessive levels of organic matter and available phosphate in paddy soils were detected in 2011. The soil available phosphate was highest in mountain foot-slope (p<0.05) and the soil organic matter was highest in diluvial terrace (p<0.05). It revealed that the soil available phosphate was the responsible factor for the differentiation of soil chemical properties by the topography in paddy soils.

Effect of Au content on the electro-catalytic activity of Pt catalyst for Pt-Au/C composite catalyst (Pt-Au/C 복합촉매에 있어서 Au 혼합비가 Pt 촉매의 활성에 미치는 영향)

  • Jo, Jin-Nyeong;Song, Jae-Chang;Song, Mink-Young;Song, Hyun-Min;Lee, Hong-Ki;Yu, Yeon-Tae
    • 한국신재생에너지학회:학술대회논문집
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    • 2010.06a
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    • pp.143.1-143.1
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    • 2010
  • 고분자 전해질막 연료전지(Polymer Electrolyte Membrane Fuel Cell; PEMFC)는 수소를 이용하여 전기를 발생시키는 친환경적이고 이상적인 발전장치로 고효율과 높은 전류밀도를 가지며 그 응용분야가 다양하다. 저온에서 작동하는 PEM fuel cell은 전극에서 효과적인 산화환원반응을 위해 그 촉매로 활성이 우수한 Pt(Platinum)을 사용하고 있으나, Pt의 높은 가격은 연료전지의 상용화에 걸림돌이 되고 있다. 본 연구에서는 연료전지의 Pt/C 촉매 층에서 Pt의 분산성을 높여 Pt의 담지량을 줄이고 작동 중 발생하는 Pt의 응집 현상을 방지하여 Pt의 수명을 연장시킬 목적으로, Au(gold) 나노입자를 첨가한 Pt-Au/C 복합나노촉매를 제조하였다. 본 발표에서는 합성된 Pt-Au/C 복합촉매 중 Au 첨가량이 Pt 촉매의 활성에 미치는 영향을 조사하기 위하여, 복합촉매 중에 금속(Pt+Au)의 총 함량이 30 wt.%와 40 wt.% 인 Pt-Au/C 촉매에 대하여 각각 Au 첨가량을 변화시켜, cyclic voltammetry 법에 의해 Au 첨가 효과를 조사한 결과에 대하여 보고하고자 한다. Au 나노입자를 제조하기 위한 출발 물질로는 $HAuCl_4{\cdot}4H_2O$를 이용하였고 trisodium citrate와 $NaBH_4$를 환원제로 하여, 입경이 5~8 nm 인 Au 콜로이드를 제조하였다. Pt-Au/C 복합나노촉매를 제조하기 위하여 먼저 Au/C 복합분체가 제조되었다. 0.03g의 carbon이 첨가된 carbon 현탁액에 합성된 Au 콜로이드 수용액을 첨가한 후 24시간 동안 교반하여 Au/C 복합분체를 제조하였다. 이 Au/C 복합분체에 $H_2PtCl_6{\cdot}6H_2O$ 수용액을 현탁하고 methanol 을 환원제로 사용해 Pt를 환원 석출시켜 Pt-Au/C 복합촉매를 제조하였다. Pt-Au/C 복합 나노촉매에서 Pt와 Au를 다양한 비율(3:1, 2.5:1.5, 2:2)로 합성하였으며 Pt-Au/C 복합촉매 중 금속(Pt+Au) 촉매의 총 함량은 30 wt.%와 40 wt.%로 각각 제조되었다. Au 나노입자 콜로이드의 분산성은 UV-visible spectrum의 흡광도에 의해 관찰되었고, Pt-Au/C 복합 나노촉매의 형상 및 분산성 분석은 transmission electron microscopy(TEM)에 의해 이루어졌다. 또한, 촉매의 전기화학적 특성평가는 cyclic voltammetry(CV)에 의해 조사되었다.

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Radioprotective Effect of Panax Ginseng in Mouse Bone-marrow (생쥐에서 방사선방호제로서의 인삼효과에 관한 연구)

  • Chae, Ki-Moon;Choi, Keun-Hee;Kim, Young-Ho;Kim, Kwang-Yoon;Bom, Hee-Seung;Kim, Ji-Yeul;Lee, Chong-Bin
    • Journal of Radiation Protection and Research
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    • v.22 no.1
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    • pp.1-7
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    • 1997
  • Radiation protection by post-irradiation injection of the ginseng extract in mice was studied. Male ICR mice, 7 weeks old, were orally injected with ginseng extrat(100mg/kg) for 10 days, and with physiologocal saline as the control. Immediately after final injection, mice were whole body irradiated with 5.08Gy(Cs-137 ${\gamma}$-ray, central dose rate : 654Gy/h) which induced Bone marrow death. At 24h after irradiation, micronucleus test and metaphase analysis in bone-marrow were carried, blood cell were counted and the survival rate were carried for 30 days after the irradiation. Stimulated recovery by the extract was observed in thrombocyte count, but that phenomenom was not showed in the erythrocyte and leucocyte counts. The 30-day survival ratio was 5% and 65% for the control and experimental group. Frequencies of micronuclei per 1000 polychromatic erythrocytes were 79.5${\pm}$1.5 in experimental group, 185.9${\pm}$35.8 in control. And Abnormal chromosomes per 50 metaphases were 112 in experimental group and 143 in control.

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Genetic Variation in Mutants Induced by Gamma Ray in Hypsizigus marmoreus (느티만가닥버섯에서 감마선에 의한 돌연변이체들의 유전적 변이)

  • Kim, Jong-Bong;Yu, Dong-Won
    • Journal of Life Science
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    • v.24 no.11
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    • pp.1174-1179
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    • 2014
  • This research was carried out to evaluate whether gamma ray is a useful tool for breeding new strains of mushrooms. For this research, 5 mutant groups, 20 strains of Hypsizigus marmoreus, 2 strains of Lyophyllum decastes, and 1 strain of Lyophyllum shimeji were used. Monokaryon spores from one variety of H. marmoreus were irradiated with 50~2,000 Gy of gamma ray. The propriety dose was 50~200 Gy for mutagenesis. Mutant monokaryon mycelia crossed each order to become dikaryon mycelia. The internal transcribed spacer (ITS) regions of rDNA were amplified using PCR, and the products were sequenced. The sequences of the ITS regions (16 partial rDNA, complete ITS1, 5.8 rDNA and partial rDNA) were analyzed by PCR, and strains of H. marmoreus, L. decastes, and L. shimeji were auto-sequenced. The lengths of the sequenced ITSs were 1,052~1,143 nucleotides. Genetic matrices were calculated using Nei-Li's genetic distance coefficient based on ITS sequence. The dissimilarities were 0~3.35% in strains of H. Hypsizigus. In addition, a phylogenetic tree was constructed based on ITS sequences using the neighbor-joining (NJ) method. The phylogenetic tree revealed that 23 strains and 5 mutant groups were divided into 12 clusters; the mutant groups fell into different clusters. These results show that mushroom spores were mutated effectively by gamma ray; therefore, gamma ray could be a useful tool for breeding new strains of mushrooms.

Effects of Thermal Effluent from Nuclear Power Plant on Growth of Sea Squirt, Haiocynthia roretzi (원자력발전소 온배수에 따른 우렁쉥이의 성장)

  • Kim Seong Gil;Kwak Hi Sang;Kang Ju Chan
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.1
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    • pp.71-76
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    • 2002
  • To understand the effect of heated effluent from a nuclear power plant on marine organism, experimental culture of Halocynthia roretzi was carried out at heated effluent of Wolsung nuclear power plant from January to December 1996. Temperature was $11.2\~27.9^{\circ}C$ and salinity was $32.54\~34.59\%_{\circ}$ during the culture period, The Growth of H. roretzi on lower area of Bonggil-ri (St. 1) was not normal in height, breadth and weight due to heated effluent. Daily growth rate (DGR) of H. roretzi about Kampo area (St, 4) was significant other station, and St. 1 was significant from other station except St. 4, Mytilus edulis was major fouling organism (over $90\%$) that were M. edulis, Dideninum moseleyi, Styela clava in experiment culture farm. St. 1 was higher (mean 143 individual) and St. 4 was lower (mean 56 individual) appearance attached of M. edulis. Growth of H. roretzi reduced when attached number of M. eduiis was increased, because correlation between DGR and number of M. edulis was negative.

Quality Characteristics of the White Birch Sap with Varying Collection Periods (자작나무수액의 유출시기별 품질특성)

  • Jeong, Su-Jeong;Lee, Chang-Hyeon;Kim, Hyun-Young;Lee, Sang-Hoon;Hwang, In-Guk;Shin, Chang-Seob;Lee, Jun-Soo;Jeong, Heon-Sang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.1
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    • pp.143-148
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    • 2012
  • This study evaluated the quality characteristics of white birch (Betula platyphylla var. japonica) sap with different collection times. The changes of browning index, turbidity, pH, total acidity, organic acid, free sugar, crude protein, crude ash, and mineral content were investigated. The browning index and turbidity increased from 0.076 to 0.222 and from 0.048 to 0.138, respectively, with increasing collection time. The pH decreased from 6.09 to 4.72, while total acidity increased with increasing collection time. Citric and malic acids were detected and malic acid increased with increasing collection time. Glucose and fructose as free sugars were detected and their contents were 0.364~0.433% and 0.497~0.664%, respectively. Crude protein and crude ash contents remarkably increased from 3.40 to 32.37 mg% and from 0.01% to 0.04%, respectively, with increasing collection time. Cu, Fe, Ca, Mg, Mn, and K were detected, and increased with increasing collection time. Particularly, K increased remarkably from 5.25 to 37.27 mg/L over time. These results indicate that the optimum processing method to improve the quality of white birch sap is necessary, because the quality of sap decreased as collection time increased, but nutritional value increased.

Effect of Length of Alkyl Group on Thermal-Liquid Crystalline Properties of Cholesteryl 4-n-Alkoxybenzoate (알킬기의 길이가 콜레스테릴 4-n-알콕시벤조에이트의 열적-액정 특성에 미치는 영향)

  • Yoon, Doo-Soo;Bang, Moon-Soo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.10
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    • pp.69-74
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    • 2017
  • In this study, cholesteryl 4-n-alkoxybenzoates (Chol-n), with alkyl groups used for controlling the temperature of transition to the liquid crystal phase, were synthesized, and the effects of the length of the alkyl groups on the physical properties of the liquid crystal compounds were investigated. The chemical structures and thermal and liquid crystalline properties of the synthesized compounds were investigated by Fourier transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance spectroscopy ($^1H$-NMR), differential scanning calorimetry (DSC), and polarizing optical microscopy (POM). The synthesized compounds showed melting transition temperatures ($T_m$) in the range of $103^{\circ}C$ to $143^{\circ}C$ and all of the compounds except Chol-6 exhibited a wide liquid crystal phase temperature range of about $60^{\circ}C$ to $100^{\circ}C$. No correlation between the number of carbon atoms in the molecule and the thermal properties of the compounds was found. All of the synthesized compounds showed an enantiotropic cholesteric phase, which was accompanied by a chiral smectic phase in the compounds Chol-6, Chol-8, Chol-9, and Chol-10. All of the compounds exhibited thermochromism in the liquid crystal state, and their color changed from red to blue as the temperature was increased.

A study on performance analysis and merging techniques of sensors in water quality measurement (수질계측센서의 성능분석 및 센서 융합기술에 관한 연구)

  • Yang Keun-Ho;Yoo Byung-Kook
    • Journal of the Institute of Convergence Signal Processing
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    • v.7 no.3
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    • pp.143-148
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    • 2006
  • There are need to manage the water quality to supply an safety to consumer through pipe and reservoir the drinking water that produced in a clean water reservoir. However, a management of water quality and monitoring in reservoir have never been performed. Recently, the government has enforced standards of water quality. However, we have a inferior technology of water quality as compared with one in USA, Japan, Germany, etc. In case of water quality inspection and analysis equipments, sensor technology is very important to improve the water quality inspection and to develop the analysis equipments. In this paper, we analyze a law and a regulation for management of drinking water quality, and propose the measurement standards of drinking water quality in pH, conductivity, residual chloride, turbidity and water temperature. Then, we analyze electrode sensors that interference within pH, conductivity, residual chloride in interference experiment.

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