To establish an effective diagnostic measure for detection of the antibodies against Actinobacillus pleuropneumoniae, the methods for tube agglutination test (TAT), plate agglutination test (PAT), micro-agglutination test(MAT) and agar-gel immunodiffusion test(ID) were improved and standarized, and the comparative studies were carried out. The results obtained through the experiments were summarized as follows. 1. The rabbit hyperimmune sera to reference serotypes 1 to 6 were cross-tested with TAT, PAT, MAT and ID. In the homologous systems, the range of antibody titers in TAT was 80 to 640, showing the cross-reaction in serotypes 3, 4, 5 and 6. The range of antibody titers in PAT was 4 to 64, showing the cross-reaction in serotypes 3, 4, 5 and 6. In ID, the range of antigen titers was 8 to 32, and cross-reaction was observed in serotype 5. 2. The optimal concentration of antigen in PAT and MAT were 100mg /ml and 1.25mg /ml respectively. The most sensitive reaction in MAT was observed in 52$^{\circ}C$ for 18hrs. 3. In ID, the most promising antigen and the buffer for agar-gel were EDTA-treated antigen and 0.05M tris buffer (pH 7.2), respectively. 4. By the tests for 200 swine sera, it was found that the frequency of positive reaction were 203 in TAT, 240 in PAT and 163 in ID. 5. When compared the titers of TAT with those of MAT for 200 swine sera, MAT showed the higher titer than TAT being increased by relative correlation. Int was found that the titer for positive readings were 20 in TAT and 40 in MAT. 6. when compared the results of ID with those of TAT for 200 swine sera, all sera with TAT titer under 10 were negative in ID. Of the sera with TAT titer 20 and 40, 55.1% nd 91.8% were positive in ID, respectively. All sera with TAT titer above 80 were positive in ID. In comparison of ID and MAT, all sera with MAT titer under 20 were negative in ID. Of the sera with MAT titer 40 and 80, 24.7% and 93.9% were positive in ID, respectively. All sera with MAT titer over 160 showed positive in ID. 7. In conclusion, the established MAT showed high sensitivity but low specificity, wherease ID revealed low sensitivity but high specificity.
When inoculating with B. bassiana 101A for the mass production of B. corpus, the infection ratio was high with regardless of the treating time with highly-humidity if the concentration of spore was 1.0${\times}$$10^8$ spores/m/, but that was low if the concentration was 1.0${\times}$$10^7$ spores/m/. In the study of the activities according to the coserving temperature or days of the B. bassiana spawn, the infection ratio of 90% was maintained for 12 days in the temperature of $4^{\circ}C$. However, the infection ratio was rapidly dropped to the below of 5% after conserved for 48 hours in the temperature of $25^{\circ}C$. Besides, the activities of the original isolate had no difference after conserved for 12 months in the temperature of $4^{\circ}C$, so that the infection ratio 90% could be mintatined. In the measure of liver-protecting activities of B. bassiana 101A, the recovering effect was 43.5% and 65.7% respectively in the poisonous treatment induced with galactosamine, compared with liver-protecting activities of Silymarin and DDB in the $H_2O$ fraction. In the poisonous treatment induced with $CCl_4$ the recovering effect was 100% and 69.3% respectively, compared with liver-protecting activities of Silymarin and DDB in the EtOAc fraction.
Titanium and its alloys are widely used as dental implants materials because of their excellent mechanical properties. However, the alumina and zirconia ceramics are preferred to use as the substitute of Ti implants because there is a problems in esthetics and biocompatibility in Ti implant. The the glass infiltrated alumina ceramics are studied to increase the toughness and biocompatibility. The 45S5 and soda-lime glass powder was mixed with ethanol at ratio of 1:1 and brushed on the surface of alumina. Then it was heat treated in the electric furnace at $1400^{\circ}C$ from 30 min. to 5 hours. The glass powder was controlled from 200 to $350{\mu}m$ using ball milling. After heat treatment, the glass infiltrated specimen was tested in universal testing machine to measure the bending strength. The surface microstructure of each specimen was observed with SEM. The biocompatibility of 45S5 and soda-lime glass coated alumina was investigated using PBS at $36.5^{\circ}C$ incubator. The specimen was immersed in PBS for 3, 5, 7, 10 days. After that, the surface morphology was investigated with SEM. As the results of experiment, the 45S5 bioglass infiltrated alumina show the increase of bending strength according to the increasing of heat treatment time from 30 min. to 5 hours at $1400^{\circ}C$ Finally the 1370N bending strength of alumina increased to 1958N at 5 hours heat treatment, which shows 1.4 times higher. In contrast to this, the soda lime glass infiltrated alumina ceramics shows the convex curve according to heat treatment time. Thus it shows maximum bending strength of 1820N at 1 hour heat treatment of $1400^{\circ}C$ It gives 1.3 times higher. However, the bending strength of soda lime glass infiltrated alumina is decreasing with increasing heat treatment time after 1 hour. The precipitation on the surface of 45S5 glass infiltrated alumina was revealed as a sodium phosphate ($Na_{6}P_{6}O_{24}6H_{2}O$) and the amount of precipitation is increasing with increasing of immersion time in PBS. In contrast to this, there is no precipitation are observed on the surface of soda lime glass infiltrated alumina. This implies that 45S5 glass infiltrated alumina brings more biocompatible when it is implanted in human body.
The high-purity germanium gamma-ray spectrometer was used to measure the radioisotope in surface water of lakes in a Chinee petroleum industrial area. 92 samples were collected from surface water of three lakes. Activity concentrations of 232Th, 226Ra and 40K in three lakes were measured, distributed in the range of 101.8-209.4, 192.1-224.9 and 335.0-548.9 mBq/L, respectively. Results were all within the limits of WHO and China. Potential environmental and health risks were assessed by calculating some radiation hazard indicators, radium equivalent index, annual effective dose, excess lifetime cancer risk, absorbed dose rate, external hazard index, internal hazard index, annual gonadal dose equivalent, activity utilization index and representative gamma index, which ranged 0.38-0.54 Bq/L, 0.06-0.08 mSv/y, 0.23 × 10-3-0.31 × 10-3, 0.17-0.24 nGy/h, 1.01 × 10-3-1.46 × 10-3, 1.55 × 10-3-2.02 × 10-3, 1.16-1.66 μSv/y, 3.13 × 10-3-4.45 × 10-3 and 2.60 × 10-3-3.77 × 10-3. The results were all at acceptable levels, meaning no impact on human health. The relationship between the electrical conductivity of surface water and the activity concentration of 232Th, 226Ra and 40K was evaluated. The electrical conductivity value was 0.241-0.369 mS/cm, showing a significant correlation coefficient between 226Ra and 40K and electrical conductivity. Multivariate statistical methods were used to determine the relationship between the activity concentrations of 232Th, 226Ra, and 40K, radiation hazard indicators and electrical conductivity.
We have investigated on the preparation of BaTiO$_3$ powder at the system of BaO$_2$-TiO$_2$-Reduction ag.-PSCA by SHS. C and Mg were used as reduction agent, and NaCl was used as PSCA (Particle Size Control Agent). The effects of the various reduction agent and its concentration, particle size control agent, reaction mixture mass on the product in the preparation of BaTiO$_3$ powder at the optimum system by SHS were investigated. The optimum conditions for reaction system and composition were BaO$_2$+TiO$_2$+0.11 Mg +0.2C+0.75NaCl in the As atmosphere. NaCl as PSCA showed the effect of controlling the particle size of product as well as the combustion temperature. The particle size of BaTiO$_3$ synthesized at the optimum condition was about 0.5 ${\mu}{\textrm}{m}$. And as the mixture mass for the reaction was increased, the more stable combustion wave appeared. We have conducted the sintering experiment at the temperature of 130$0^{\circ}C$, the time of 2 h and the atmosphere of air to measure the dielectric properties of BaTiO$_3$ synthesised in this work, and the sample sintered in this condition showed 2,290 of dielectric constant at 10$0^{\circ}C$ and 13,890 at curie point (129$^{\circ}C$).
본 연구는 균질 및 2개층 비균질지반에서 사질토지반 상에 놓인 정방형 기초의 극한지지력과 침하에 대하여 고찰하였다. 본 연구는 얕은기초의 거동에 대한 정방형 기초의 크기, 지반 상대밀도, 기초 폭에 대한 상부층의 두께 비(H/B), 상부층 아래 경계면의 경사($\theta$) 그리고 지반강성비의 영향을 규명하기 위하여 모형실험을 수행하였다. 동일 상대밀도에서 지지력 계수($N_{{\gamma}}$)는 일정하지 않으며 기초 폭에 직접적으로 관련되며 지지력계수는 기초 폭이 증가함에 따라 감소하였다. 기초크기의 영향과 구속압력의 영향을 고려하는 Ueno 방법에 의한 극한지지력의 예측값은 고전적인 지지력 산정식보다 더 잘 일치하며 그 값은 실험값의 65% 이상으로 나타났다. $\theta$=$0^{\circ}$인 2개층 지반의 결과에 근거하여, 극한지지력에 대한 하부층 지반의 영향을 무시할 수 있는 한계 상부층 두께는 기초 폭의 2배로 결정되었다. 그러나, 73%의 상부층 상대밀도인 경우는 침하비($\delta$B) 0.05 이하에서만 이 결과가 유효하였다. 경계면이 경사진 2개층 지반의 결과에 근거하여, 상부층의 상대밀도가 느슨할수록 그리고 상부층의 두께가 클수록 극한지지력에 대한 경계면 경사의 영향은 크지 않는 것으로 나타났다. 경계면의 경사가 증가함에 따른 극한침하량의 변화는 경계면이 수평인 경우($\theta$=$0^{\circ}$)를 기준으로 0.82~1.2(상부층 $D_{r}$=73%인 경우) 그리고 0.9~1.07(상부층 $D_{r}$=50%인 경우) 정도로 나타났다.Markup Language 문서로부터 무선 마크업 언어 문서로 자동 변환된 텍스트를 인코딩하는 경우와 같이 특정한 응용 분야에서는 일반 문자열에 대한 확장 인코딩 기법을 적용할 필요가 있을 수 있다.mical etch-stop method for the etching of Si in TMAH:IPA;pyrazine solutions provides a powerful and versatile alternative process for fabricating high-yield Si micro-membranes. the RSC circle, but also to the logistics system in the SLC circle. Thus, the RSLC model can maximize combat synergy effects by integrating the RSC and the SLC. With a similar logic, this paper develops "A Revised System of Systems with Logistics (RSSL)" which combines "A New system of Systems" and logistics. These tow models proposed here help explain several issues such as logistics environment in future warfare, MOE(Measure of Effectiveness( on logistics performance, and COA(Course of Actions) for decreasing mass and increasing velocity. In particular, velocity in logistics is emphasized.
Previous studies have shown that bone marrow mesenchymal stromal cell (MSC) transplantation significantly improves the recovery of neurological function in a rat model of intracerebral hemorrhage. Potential repair mechanisms involve anti-inflammation, anti-apoptosis and angiogenesis. However, few studies have focused on the effects of MSCs on inducible nitric oxide synthase (iNOS) expression and subsequent peroxynitrite formation after hypertensive intracerebral hemorrhage (HICH). In this study, MSCs were transplanted intracerebrally into rats 6 hours after HICH. The modified neurological severity score and the modified limb placing test were used to measure behavioral outcomes. Blood-brain barrier disruption and neuronal loss were measured by zonula occludens-1 (ZO-1) and neuronal nucleus (NeuN) expression, respectively. Concomitant edema formation was evaluated by H&E staining and brain water content. The effect of MSCs treatment on neuroinflammation was analyzed by immunohistochemical analysis or polymerase chain reaction of CD68, Iba1, iNOS expression and subsequent peroxynitrite formation, and by an enzyme-linked immunosorbent assay of pro-inflammatory factors (IL-$1{\beta}$ and TNF-${\alpha}$). The MSCs-treated HICH group showed better performance on behavioral scores and lower brain water content compared to controls. Moreover, the MSC injection increased NeuN and ZO-1 expression measured by immunochemistry/immunofluorescence. Furthermore, MSCs reduced not only levels of CD68, Iba1 and pro-inflammatory factors, but it also inhibited iNOS expression and peroxynitrite formation in perihematomal regions. The results suggest that intracerebral administration of MSCs accelerates neurological function recovery in HICH rats. This may result from the ability of MSCs to suppress inflammation, at least in part, by inhibiting iNOS expression and subsequent peroxynitrite formation.
Kim, Sung Ho;Lee, Bu Hyung;Kwon, Soo Il;Kim, Jae Seok;Kim, Gi-sub;Park, Min Seok;Jung, Haijo
Progress in Medical Physics
/
v.27
no.3
/
pp.156-161
/
2016
When air discharged from a radioisotope production facility is contaminated with radiation, the public may be exposed to radiation. The objective of this study is to manage such radiation exposure. We measured the airborne radioactivity concentration at a 30 MeV cyclotron radioisotope production facility to assess whether the exhaust gas was contaminated. Additionally, we investigted the radioactive contamination of the air filter for efficient air purification and radiation safety control. To measure the airborne radiation concentration, specimens were collected weekly for 4 h after the beginning of the radioisotope production. Regarding the air purifier, five specimens were collected at different positions of each filter-pre-filter, high-efficiency particulate air filter, and charcoal filter-installed in the cyclotron production room. The concentrations of F-18, I-123, I-131, and Tl-201 generated in the radioiodine production room were $13.5Bq/m^3$, $27.0Bq/m^3$, $0.10Bq/m^3$, and $11.5Bq/m^3$, respectively; the concentrations of F-18, I-123, and I-131 produced in the radioisotope production room were $0.05Bq/m^3$, $16.1Bq/m^3$, and $0.45Bq/m^3$, correspondingly; and those of F-18, I-123, I-131, and Tl-201 generated in the accelerator room were $2.07Bq/m^3$, $53.0Bq/m^3$, $0.37Bq/m^3$, and $0.15Bq/m^3$, respectively. The maximum radiation concentration of I-123 generated in the radioiodine production room was 1,820 Bq/g, which can be disposed after 2 days. The maximum radiation concentration of Tl-202 generated in the radioisotope production room was 205 Bq/g, and this isotope must be stored for 53 days. The I-123 generated in the radioiodine production room had a maximum concentration of 1,530 Bq/g and must be stored for 2 days. The maximum radiation concentration of Na-22 generated in the radioisotope production room was 0.18 Bq/g and this isotope must be disposed after 827 days. To manage the exhaust, the efficiency of air purification must be enhanced by selecting an air purifier with a long life and determining the appropriate replacement time by examining the differential pressure through systematic measurements of the airborne radiation contamination level.
Magazine of the Korean Society of Agricultural Engineers
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v.17
no.1
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pp.3627-3641
/
1975
The Government is trying to increase total food grain yield to meet national self sufficiency by means of increasing unit yield as well as extending crop land, and this year he set the target of 321,000 hectare of forest to clear for cropping. This study was carried to investigate the most efficient method of clearing hillock by bulldozer, and successful method to develope yielding potential of newly cleared land in short term. Since the conventional land clearing method is just earth leveling and root removing neglecting top soil treatment, the growth of crop was poor and farmer tends not to care the land. The top-soil-furrowing method is applied through out this study, that is advantageous especially for the land having shallow top soil and low fertility like Korean forest. In this study, various operating method were tried to find out most efficient method separately in connection with the land slope less than 25 percent and over, and several fertilizing methods to develop yielding potential. The results are as follows; 1) For the natural land slope utilization method, applicable to the land having less than 25 percent slope, reverse operating was more efficient than using forward gear of bulldozer. The operating time was 3 hour 32 minutes and 36 seconds using forward gear was 2 hour 32 minutes and 30 seconds for reverse gear operation per 1,000 square meter. 2) Bulldozer having angle blade adjustment needed 7hr 15min. for constructing of terrace per 10a compaire with the one having angle & tilt adjustment needed 6hr 4min for same operations. Specially there is significant difference for operation time of first period (earth cutting) such as bulldozer having angle blade adjustment needed 3hr 56min compaired with the one having angle & tilt adjustment 3hr 59min. In construction of terrace, the bull-dozer having tilting and angle blade adjustment was most suitable and performed efficiently. 3) For the fertilizer application treatment, the grass (Ladino clover) yield in first year was almost same as ordinary field's in the plot applied(N.P.K+lime+manure) while none fertilizer plot showed one tenth of it, and (N.P.K.+lime) applied plot yielded on third. 4) The effect of different land clearing method to yield showed significant difference between each treatment especially in the first year, and the conventional method was the lowest. In the second year, still conventional terracing plot yielded only half of ordinary field while the other plots showed as same as ordinary field's. 5) The downward top soil treatment plot showed most rapid improvement in soil structure during one year physio chemically, it showed increase in pH rate and organic composition, and the soil changed gradually from loam to sand-loam and the moisture content increased against the pF rate, and it gives good condition to grow hay due to the increase of field water capacity with higher available water content. 6) Since the soil of tested area was granite, the rate of soil errosion was increased about 2 to 5 percent influencing in soil structure more sand reducing clay content, and an optimum contour farming method should be prepared as a counter measure of errosion.
Sonic nozzles have been a standard device for measurement of steady state gas flow, as recommended in ISO 9300. This paper introduces two sonic nozzles of diameter ${\Phi}$ 0.03 mm and ${\Phi}$ 0.2 mm precisely machined according to ISO 9300. The constant volume flow meter(CVFM), readily set up in the Vacuum center of KRISS. was used to calibrate the discharge coefficients of both nozzles. The calibration results were shown to determine them within the 3% expanded measurement uncertainty. Calibrated sonic nozzles were found to be applicable for precision measurement of steady state gas flow in the vacuum process in the ranges of 0.6~1,800 cc/min. Those flow conditions are equivalent to the fine gas flow with Reynolds numbers of 26~12,100. Those encouraging results confirm that calibrated sonic nozzles enable precision measurement of extremely low gas flow encountered very often in th vacuum processes. Both calibrated sonic nozzles are proven to provide the precision measurement of the volume flow rate of the dry vacuum pump within one percent difference in reference to CVFM. Calibrated sonic nozzles are applied to a new 'in-situ and in-field' equipment designed to measure the volume flow rate of vacuum pumps in the semiconductor and flat display processes. Furthermore, they can provide other applications to flow control devices in vacuum, such as MFC, etc.
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