• 고려인삼학회:학술대회논문집
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• 고려인삼학회 2002년도 학술대회지
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• pp.491-501
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• 2002

A cross-examination between KT&G Central Research Institute and Guangzhou Institute for Drug Control was carried out in order to select optimum conditions for extraction, separation and determination of ginsenosides in red ginseng and to propose a better method for the quantitative analysis of ginsenosides. The optimum extraction conditions of ginsenosides from red ginseng were as follows: the extraction solvent, $70\%$ methanol; the extraction temperature, $100^{\circ}C;$ the extraction time, 1 hour for once; and the repetition of extraction, twice. The optimum separation conditions of ginsenosides on the SepPak $C_{18}$ cartridge were as follows: the loaded amount, 0.4 g of methanol extract; the washing solvents, distilled water of 25 ml at first and then $30\%$ methanol of 25 ml; the elution solvent, $90\%$ methanol of 5 ml. The optimum HPLC conditions for the determination of ginsenosides were as follows: column, Lichrosorb $NH_2(25{\times}0.4cm,$ 5${\mu}m$, Merck Co.); mobile phase, a mixture of acetonitrile/water/isopropanol (80/5/15) and acetonitrile/water/isopropanol (80/20/15) with gradient system; and the detector, ELSD. On the basis of the optimum conditions a method for the quantitative analysis of ginsenosides were proposed and another cross-examination was carried out for the validation of the selected analytical method conditions. The coefficient of variances (CVs) on the contents of ginsenoside-$Rg_{1}$, -Re and $-Rb_1$ were lower than $3\%$ and the recovery rates of ginsenosides were $89.4\~95.7\%,$ which suggests that the above extraction and separation conditions may be reproducible and reasonable. For the selected HPLC/ELSD conditions, the CVs on the detector responses of ginsenoside-Rg, -Re and $-Rb_1$) were also lower than $3\%$, the regression coefficients for the calibration curves of ginsenosides were higher than 0.99 and two adjacent ginsenoside peaks were well separated, which suggests that the above HPLC/ELSD conditions may be good enough for the determination of ginsenosides.

• 한국작물학회지
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• 제27권3호
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• pp.276-282
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• 1982

본 시험은 1981년에 참깨품종 "수원 9호"를 Pot에 공시하여 토양의 배수력 차이에 따른 참깨의 생육과 수량형성의 차이를 구명하고자 시도되었다. 즉 점토와 세사를 용량비(V/V Ratio)로 각각 0:100, 25:75, 50:50, 75:25 및 100:0가 되도록 혼합한 토양에 6일 간격 20mm 관수와 3일 간격 10mm 관수의 효과와 반응차이를 해석하였다. 토양의 용량비에 따른 수분의 배수성은 점토혼합율에 따라 거의 직선적으로 감소하는 경향이었다. 토양배수성 차이에 따른 참깨의 생육반응은 순세사토에서만 한해가 인정되므로 관수의 증대가 요구되었고 그 외의 배수조건의 차이에 따른 생육반응에는 통계적 유의차가 없었다. 수량구성요소 가운데서 수량에 기여도가 가장 컸던 것은 주당삭수로서 수량과 마찬가지로 점토와 사토가 75%:25%로 혼합된 조건 즉 배수성보다는 보습성이 양호한 토양에서 가장 많았으며 삭당입수나 등숙성은 선행형질인 주당삭수와의 부적상관 때문에 보습성 보다는 배수성이 양호한 토양에서 증대되는 경향을 나타내었다. 관수방법간에 이들 수량구성 특성의 차이는 없었으나 수량은 6일 간격 20mm보다 3일 간격 10mm 관수로서 보습력(점사율 75%:25%)이 높은 조건에서 최다수준이었으며 과도한 배수성이나 보습성의 조건에서는 뚜렷한 감수의 경향이었다.감수의 경향이었다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권5호
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• pp.661-671
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• 2006

Steers (n = 335) of known genetic backgrounds from four fundamentally different growth types were subjected to two production systems to study the main effects and possible interactive effects on carcass composition. Growth types were animals with genetic potential for large mature weight (LL), intermediate mature weight-late maturing (IL), intermediate mature weight-early maturing (IE), and small mature weight-early maturing (SE). Each year, in a nine year study, calves of each growth type were weaned and five steers of each growth type were developed on pasture or feedlot and harvested at approximately 20 and 14 mo of age, respectively. Data recorded were chilled carcass weight and percentages of forequarter, foreshank, chuck, rib, plate, brisket, hindquarter, round, rump, shortloin, sirloin, flank, lean, fat, bone, and retail cuts. The growth $type{\times}production$ system interaction was an important source of variation in chilled carcass weight (p = 0.0395) and percentage retail cuts (p = 0.001), lean (p = 0.001), fat (p = 0.001), rump (p = 0.0454), shortloin (p = 0.0487), and flank (p = 0.001). The ranking of the growth $type{\times}production$ system means for percentage lean was LL-pasture>IL-pasture = IE-pasture = SE-pasture>LL-feedlot, IL-feedlot>IE-feedlot = SE-feedlot. The growth $type{\times}production$ system interaction was non-significant (p>0.05) for forequarter, foreshank, chuck, rib, plate, brisket, hindquarter, round and bone. Growth types of IE and SE yielded greater (p<0.05) mean forequarter than did growth types of IL and LL ($51.6{\pm}0.3$ and $51.5{\pm}0.3$ vs. $51.1{\pm}0.3$ and $50.8{\pm}0.3%$). Mean bone was highest (p<0.05) for the LL growth type and lowest (p<0.05) for the SE growth type ($19.5{\pm}0.5$ vs. $16.8{\pm}0.5%$). Mean bone was greater (p<0.05) for the pastured steers than for the feedlot steers ($21.8{\pm}0.8$ vs. $14.5{\pm}0.6%$). These data indicate that growth type responded differently in the two production systems and that these results should be helpful in the match of genetics to production resources.

• Asian-Australasian Journal of Animal Sciences
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• 제20권6호
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• pp.994-1001
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• 2007

Changes in shear force value, transverse sections, myofibrils and intramuscular connective tissue of bovine skeletal muscle exposed to the combination of high-pressure up to 400 MPa and heat (30 and $60^{\circ}C$) were studied. The shear force value decreased by pressure-heat treatment up to 200 MPa at 30 and $60^{\circ}C$, and then slightly increased over 200 MPa at $30^{\circ}C$. Shear force values of treated muscles were lower than those of untreated ones. Gaps between muscle fibers in the untreated muscle were a little clear, and then they became very clear in the treated muscles up to 200 MPa at 30 and $60^{\circ}C$. However, the gaps reduced significantly over 200 MPa at $30^{\circ}C$. The remarkable rupture of I-band and loss of M-line materials progressed in the myofibrils with increasing pressure applied. However, degradation and loss of the Z-line in myofibrils observed in the muscle treated at $60^{\circ}C$ was not apparent in the muscle treated at $30^{\circ}C$. The length of the sarcomere initially contracted by pressure-heat treatment of 100 MPa at $30^{\circ}C$ seemed to have recovered with increase of the pressure up to 400 MPa. In the muscle treated at $60^{\circ}C$, the length of sarcomere gradually decreased with increase of the pressure up to 400 MPa. In the treated muscles, changes in the honeycomb-like structure of endomysium were observed and accelerated with increase of the pressure. A wavy appearance clearly observed at the inside surface of endomysium in the untreated muscles gradually decreased in the treated muscles with increase of the pressure. Tearing of the membrane was observed in the muscles treated over 150 MPa at $30^{\circ}C$, as observed in the sample pressurized at 100 MPa at $60^{\circ}C$. The roughening, disruption and fraying of the membrane were observed over 200 MPa at $60^{\circ}C$. From the results obtained, the combination of high-pressure and heat treatments seems to be effective to tenderize tough meat. The shear force value may have some relationship with deformation of intramuscular connective tissue and myofibrils.

• 한국지반공학회논문집
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• 제31권5호
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• pp.35-46
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• 2015

본 연구에서는 최근 국내에서 사용이 증가하고 있는 2열 자립식 흙막이 공법에 있어서 안정성에 영향을 미치는 주요 설계인자들을 분석하고 설계기준을 제안하기 위하여 현장적용 결과의 분석 및 3차원 유한차분 해석을 수행하였다. 지반특성에 따른 본 공법의 거동을 분석하기 위하여 사질토가 지배적인 현장과 점성토가 지배적인 2개의 현장에 적용을 수행하였으며, 굴착에 따른 흙막이 벽체의 수평변위 및 휨모멘트를 분석하였다. 3차원 유한차분 모델링 기법의 타당성을 검증하기 위하여 현장적용 결과와 비교 분석을 수행한 결과, 본 연구의 수치해석 모델링 기법은 본 공법의 굴착에 따른 거동을 합리적으로 모사하는 것으로 나타났다. 또한 흙막이 벽체를 구성하는 전열말뚝(엄지말뚝) 및 후열말뚝(억지말뚝)의 간격(S), 전열말뚝과 후열말뚝간의 거리(D), 굴착심도(H) 및 말뚝의 근입깊이(Z) 등, 본 공법의 주요 설계인자들의 영향 정도를 분석하기 위하여 다양한 경우의 3차원 유한차분 모델링 및 해석을 수행하였다. 그 결과, 굴착에 따라 발생하는 흙막이 벽체의 최대 수평변위는 전열말뚝 및 후열말뚝의 간격의 감소, 전열말뚝과 후열말뚝간의 거리의 증가 및 말뚝 근입심도의 증가에 따라 감소하였으며, 이러한 특징은 점성토 조건의 지반보다는 사질토 조건의 지반에서 보다 명확하게 나타나는 것을 확인할 수 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제28권1호
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• pp.86-94
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• 2015

This experiment was conducted to evaluate the chemical composition and amino acid (AA) digestibility of sunflower seed meal (SFSM) and to use this data to develop prediction equations for estimating AA digestibility for growing pigs. Ten SFSM were collected from five provinces in China. Twelve barrows ($38.8{\pm}4.6kg$), fitted with ileal T-cannula were allotted into two $6{\times}6$ Latin square designs. Each of six experimental periods comprised a 5-d adaption period followed by a 2-d collection of ileal digesta. The ten test diets contained 50% SFSM as the sole source of AA. Another nitrogen-free diet was used to measure the basal endogenous losses of crude protein (CP) and AA. Chromic oxide (0.3%) was used as an inert marker in each diet. There was considerable variation (CV>10%) among the ten SFSM in chemical composition (dry matter [DM]). The concentration of CP and ether extract (EE) ranged from 29.33% to 39.09% and 0.88% to 11.33%, respectively. Crude fibre (CF), neutral detergent fibre and acid detergent fibre ranged from 21.46% to 36.42%, 38.15% to 55.40%, and 24.59% to 37.34%, respectively. There was variation among the ten SFSM in apparent ileal digestibility (AID) and standardized ileal digestibility (SID) for lysine and threonine, which ranged from 63.16 to 79.21 and 55.19% to 72.04% for AID and 67.03% to 82.07% and 61.97% to 77.01% for SID, respectively. The variation in CP and methionine ranged from 60.13% to 74.72% and 74.79% to 88.60% for AID and 66.70% to 79.31% and 77.16% to 90.27% for SID, respectively. Methionine was a good indicator to predict AA digestibility. These results indicate that conventional chemical composition of SFSM was variable (CV>10%) among the ten SFSM (DM). The results of AID, SID and prediction equations could be used to evaluate the digestibility of SFSM in growing pigs.

• Asian-Australasian Journal of Animal Sciences
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• 제22권4호
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• pp.507-515
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• 2009

A series of experiments were conducted to determine the suitable freezing and thawing temperatures for the freezing of boar semen in 5 ml maxi-straws. The ultrastructure, in vitro fertilization (IVF) and artificial insemination (AI) of frozen-thawed semen were also be evaluated. The 5 cm freezing height gave the best results not only in post-thaw motility rate (54.00%), but also in normal acrosome morphology rate (NAR) (80.23%). There was no significant difference in the post-thaw motility between different thawing temperatures and corresponding thawing times (p>0.05); the group of $52^{\circ}C$ and 25 s gave the highest motility rate (45.00%). As a whole, not only from the motility but also the NAR, thawing at $42^{\circ}C$ was better than the other two treatments. In the freezing packages, 5 ml maxi-straw gave a little lower mobility (40%), viability rate (49.58%), plasma membrane integrity rate (53.91%) and NAR (52.65%) than the 0.25 ml straw, but there was no significant difference between the two straw volumes (p>0.05). The IVF capacity of frozen-thawed semen in this experiment was similar to fresh semen. From ultrastructure observation, the main damage to boar spermatozoa after freezing was seen in the acrosome, such as swelling and formation of vesicles. After AI in recipient Shanghai White sows, frozen-thawed semen from 5 ml maxi-straws and pellets produced 72.2% and 80% conception rate and 7.8 and 8 litter sizes, respectively, and there was no significant difference between the 5 ml maxi-straw and the pellet (p>0.05).

• Asian-Australasian Journal of Animal Sciences
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• 제29권12호
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• pp.1768-1773
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• 2016

This experiment was conducted to determine the effect of variety and planting year on the nutritive values of corn fed to growing pigs. Four corn varieties examined in this experiment were planted in the same village located in Longhua County, Heibei Province, China, in 2012, 2013, and 2014, respectively. During each year, corn was hand-harvested in early October and sun dried to about 14% moisture content. Three batches of twenty-four barrows ($33.27{\pm}4.30$, $31.88{\pm}2.93$, $34.21{\pm}3.81kg$ body wight [BW] in 2012, 2013, and 2014, respectively) were used and allotted to a complete block design with 4 diets and 6 replicate pigs per diet. Pigs were individually placed in metabolic crates. The four experimental diets were formulated by mixing each variety of corn and vitamins and minerals, respectively. A five-day collection period followed a seven-day diet acclimation period. The results indicated that variety of corn significantly influenced the available energy content (digestible energy [DE] on dry matter basis, p<0.05; metabolizable energy (ME) on dry matter basis, p<0.05, respectively), and the apparent total tract digestibility (ATTD) of organic matter (p<0.01), dry matter (p<0.05), gross energy (p<0.05), neutral detergent fiber (p<0.01), acid detergent fiber and ether extract (p<0.05). The planting year also significantly influenced the available energy contents (DE on dry matter basis, p<0.05; ME on dry matter basis, p<0.01, respectively) and the ATTD of neutral detergent fiber (p<0.01), acid detergent fiber (p<0.01), crude protein (p<0.01), and ether extract (p<0.01). No interaction was observed between the variety and planting year in DE and ME contents in corn. In conclusion, the variety and planting year significantly influenced the available energy and nutrient digestibility of corn fed to growing pigs.

• 한국응용곤충학회지
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• 제56권4호
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• pp.365-370
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• 2017

생대추가 신소득 작목으로 부각되면서 재배지가 확대되고 있으나 우리나라 장마기와 최근 이상기후로 인해 대추 수분 수정 및 착과에 문제가 발생하고 있는 실정이다. 이에 생대추 재배를 위해 도입된 비가림하우스에서 서양종 꿀벌과 서양뒤영벌을 이용하여 화분매개곤충의 활동 특성과 착과 효율에 어떠한 영향을 미치는지 구명하고자 본 시험을 수행하였다. 대추 복조 품종을 대상으로 비가림하우스에 망실을 씌워 서양종 꿀벌과 서양뒤영벌을 2016년 6월 1일부터 7월 25일까지 약 55일정도 방사하였다. 서양종 꿀벌은 주로 오후에 활동이 많았으나 서양뒤영벌의 활동은 시간에 관계없이 다소 균일하였다. 대추 신초 가지의 착과율을 조사한 결과 방화곤충을 차단한 무처리 5.5% 대비 서양종 꿀벌은 10.2%, 서양뒤영벌은 8.9%로 대추 비가림하우스내 화분매개곤충 활용시 착과량 증진으로 농가 소득증대에 기여할 것으로 생각된다.

• 한국독어학회지:독어학
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• 제3집
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• pp.35-55
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• 2001

In der Semiotik ist es sehr wichtig, den Rahmenbereich des Zeichens festzustellen, weil der Bereich des Zeichens je nach Wissenschaftler unterschiedlich ist. Dementsprechend muss jedes Forschungsergebnis in der Semiotik hinterfragt werden, um sichergehen zu $k\"{o}nnen$, ob es zur Semiotik $geh\"{o}rt$ oder nicht. Daher besteht $zun\"{a}chst$ die Notwendigkeit, zu bestimmen, was ein Zeichen ist. In diesem Zusammenhang versuchten wir in diesem Aufsatz, damit wir den Begriff 'Zeichen' besser erfassen $k\"{o}nnen$. das Zeichen von C. S. Peirce mit dem van W. v. Humboldt zu vergleichen. Zu Beginn dieses Aufsatzes stellten wir dar, was ein Zeichen ist. Dieser Vorgang ist notwendig, damit mit Hilfe des allgemeinen Begriffes des Zeichens ein Vergleich zwischen Peirce und Humboldt angestellt werden kann. Danach stellten wir die Zeichentheorie von Peirce vor. Nach Peirce ist die Sprache eine Untergattung von drei Zeichensorten, die Ikon, Index und Symbol genannt werden. Nach ihm gehbrt das Symbol zur Sprache. $F\"{u}r$ ihn ist der Interpretant im Zeichenbegriff am wichtigsten, weil ein Zeichen immer eines Interpretantes bedarf, der das Zeichen interpretiert. Aber der Interpretant ist kein Individuum, sondern ein interpretierendes $Bewu{\ss}tsein$, also eine traditionelle Gesellschaft, die ein Muster zum Interpretieren bietet. $Anschlie{\ss}end\;besch\"{a}ftigten$ wir uns mit der Theorie von Humboldt. Er benutzt verschiedene Zeichenbegriffe wie z.B. Schriftzeichen, Lautzeichen, Sprachzeichen, grammatische Zeichen, $h\"{o}rbare$ Zeichen, zeitliche Zeichen und $r\"{a}umliche$ Zeichen. Bei ihm ist die Unterscheidung zwischen Wort und Zeichen besonders wichtig, weil das Wort nicht zum Zeichen $geh\"{o}rt$, wie wir es normalerweise annehmen. Er behauptet, dass das Wort beim Produzieren motiviert ist. Daher ist nach ihm das Wort transsemiotisch. $Dar\"{u}ber$ hinaus muss das Zeichen und das Wort im Zusammenhang zwischen Sprache und Denken betrachtet werden. Wenn man das Wort als Zeichen betrachtet, ist das Wort ein Zwischending zwischen dem Sprechenden und dem Ding. Damit $k\"{o}nnen$ wir schlussfolgern; 1) Bei Humboldt ist das Zeichen em Mittel zum Denken, das sich vom transsemiotischen Wort unterscheidet. 2) Nach Peirce besteht ein Zeichen aus Ikon, Index und Symbol. Im Zeichen, das aus Reprasentamen, Interpretant und Objekt besteht, ist der Interpretant am wichtigsten, denn ein Zeichen, dass keinen Interpretant hat, ist kein Zeichen mehr, das interpretierbar ist.