• 한국식품위생안전성학회지
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• 제22권3호
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• pp.151-158
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• 2007

동치미로부터 분리한 유산균 (68 균주) 중 Escherichia coli O157에 대한 항균 효과를 나타내는 균주는 Lactobacillus plantarum K11로 동정되었다. 분리균주 La. piantarum K11이 생산한 박테리오신의 항균 활성은 대수증식기 후반부에 12,800 BU/mL로 최대 활성에 이르렀다. 항균 활성은 pepsin, protease, proteinase K, papain, chymotrypsin 및 trypsin 처리에 의해 완전히 소실되었으나, catalase, ${\alpha}-amylase$, lysozyme 및 lipase에 의해서는 영향을 받지 않았으므로 단백질성 물질임을 확인하였다. 게다가, 이 활성은 pH 3.0-9.0의 조건하에서나 -20, 4 및 $25^{\circ}C$에서 30일간의 저장 동안에도 안정하였다. 또한 $100^{\circ}C$에서 30분간 가열처리에도 비교적 안정한 편이었고, chloroform이나 hexane 처리에도 활성에 변함이 없었다. 분리 균주의 박테리오신은 Bacillus spp., Listeria spp. 및 Staphylococcus spp. 등의 일부 식중독균의 억제효과는 나타나지 않았으나, Enterobacter aerogenes와 E. coli 등의 장내세균의 억제에는 효과적이었으며, 특히 640 BU/mL의 박테리오신 처리에 의해서 10시간 배양만에 E. coli O157이 완전하게 사멸되었다.

• Journal of Animal Science and Technology
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• 제62권1호
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• pp.84-93
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• 2020

An experiment was conducted to evaluate Pediococcus spp.-fermented chicken meat as a snack for dogs. The fermented or non-fermented snacks used in this study were prepared through the following process; meat mixtures containing 52.8% MDCM, 35.2% chicken breast meat (CBM) and 9.7% corn starch were inoculated with or without Pediococcus spp., incubated at 37℃ for 24 h and then sterilized at 121℃ for 20 min. During the 24-h fermentation, the pH of fermented chicken snack dropped rapidly with concomitant increase in number of lactic acid bacteria. The nutritional composition was not altered by fermentation. In vitro pepsin nitrogen digestibility was higher (p < 0.05) in the fermented snack compared with the non-fermented snack. Upon storage at room temperature for 14 days, bacteria grew slowly in fermented vs. non-fermented snack samples. In a palatability trial, dogs preferred non-fermented over fermented snack food. In 12-d-long feeding trial, fecal ammonia content was lowered, but fecal lactic acid content was increased in dogs fed the fermented vs. non-fermented snack food. Our study shows that the fermented MDCM-based snack exhibited good preservability upon storage, and improved in vitro nitrogen digestibility and fecal characteristics in dogs.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.115.1-115
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• 2003

Post-harvest decay, caused by Penicillium spp. is a serious problem of fruits worldwide. Morphological characteristics and molecular markers were used to characterize 22 Penicillium isolates from apples, 18 isolates from pears, 60 from oranges and 18 from grapes and 23reference isolates representing related Penicillium spp. to assess their diversity and resolve their taxonomy. Based on morphological and physiological characteristics, the isolates were grouped as identical or very similar to P. digitatum, P. italicum, P. ulaiense or very similar to P. crustosum, P. expansum, P. solitum and unidentified Penicillium spp. Based on sequence comparisons of ITS region, variable site were presented within and among the species, but there variation were not correlated with the species. Cluster analyses of AP-PCR fragment patterns using UP and L45 primer and the -tubulin gene sequence, the Penicillium species were segregated into distinct groups. Particularly. the -tubulin partial sequence data provided support for species concepts based on morphological and physiological characteristics.

• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 2004년도 제34차 추계 국제 학술대회
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• pp.384-387
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• 2004

겨울철 토양에서 ${\beta}-Galactosidase$를 생산하는 균주를 분리하였으며 동정한 결과 그람 음성 간균이고 Pantoea spp. 로 확인되었다. Pantoea spp. 균주의 세포 추출물로부터 DEAE-Sephacel chromatography와 affinity chromatography를 이용하여 ${\beta}-Galactosidase$를 분리하였다. Pantoea spp. 의 ${\beta}-Galactosidase$의 반응 최적 온도는 $45^{\circ}C$이이고 최적 pH는 $5.5{\sim}7.5$이고 열안정성을 조사한 결과 $45^{\circ}C$이상의 온도에서 불활성 되는 것으로 나타났고 E. coli에서 분리된 효소보다 저온에서의 활력이 좋았지만 상업적인 효소인 Kluyveromyces lactis (Validase) 보다는 낮았다.

• Journal of Microbiology and Biotechnology
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• 제15권4호
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• pp.716-721
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• 2005

The role of an effective microbial species is critical to the successful application of biological processes to remove sulfur compounds. A bacterial strain was isolated from the soil of a malodorous site and identified as Burkholderia spp. This isolate was able to oxidize thiosulfate to sulfate, with simultaneous pH decrease and accumulation of elemental sulfur. The specific growth rate and the sulfate oxidation rate using the thiosulfate basal medium were $0.003 h^{-1}\;and\;3.7 h^{-1}$, respectively. The isolated strain was mixotrophic, and supplementation of $0.2\%$ (w/v) of yeast extract to the thiosulfate-basal medium increased the specific growth rate by 50-fold. However, the rate of sulfate oxidation was more than ten times higher without yeast extract. The isolate grew best at pH 7.0 and $30^{\circ}C$, and the sulfate oxidation rate was the highest at 0.12 M sodium thiosulfate. In an upflow biofilter, the isolated strain was able to degrade $H_2S\;with\;88\%$ efficiency at 8 ppm and 121/h of incoming gas concentration and flow rate, respectively. The cell density at the bottom of the column reached $3.2{\times}10^8$ CFU/(g bead) at a gas flow rate of 121/h.

• 한국미생물·생명공학회지
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• 제10권3호
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• pp.191-196
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• 1982

미곡저장중 균류에 의한 미곡의 손실을 방지하기 위하여 1978년 12월 부터 1979년 6월까지 7개월간 싸일로, 평창고 및 통가리에 저장한 미곡시료(밀양23호)에서 균류를 분리, 동정하였다. 한국산 벼 및 현미에서 분리된 30종의 균류중, Aspergillus속 11종(A. caespitosus, A. condidus, A. chevalieri, A fischeri, A. fumigatus, A. fiavus, A. nidulans, A. oryzae, A. ruber, A. sydowii, A. versicolor), Penicillium 속 5 종 (P. atramentosum P. chrysogenum, P. cyaneofulvum, P. nototum, P. steckii), Alternaria 속 2종 (Al. faesiculata, Al. grisea) Curvalaria 속 2종 (C, interseminata, C. tetromea), Trichothecium roseum, Nigrospora sphaerica, Rhizopus nigricans, Fusarium spp., Mucor spp., Helminthosporium spp., 및 Gliocladiopsis spp. 각 1종, 총 27종이 동정, 확인되었다. 한국산 벼에서 분리된 표면균류는 A. flavus와 A. candidus가 대부분이었으며, 현미에서는 A. sydowii가 많이 출현하였다. 또한 저장중 변질미에는 A. candidus, A. versicolor 및 A. glaucus group들이 주로 많았다.

• 미생물학회지
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• 제7권3호
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• pp.107-114
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• 1969

Aspergillus을 이용하요 생리적 제성질을 조사하였던 바 다음과 같은 결론을 얻었다. 1) 각 균주들은 각각 그들의 특성을 가지고 있었으며 이로서 균동정의 가능성을 나타내었다. 2)Amilase측정결과에서 보면 비교적 역가가 높은 균주들이 관찰되었으며, 이는 일주문의 경과에 따라 역가가 증가하였으나 protease의 역가는 우수한 균주를 발견키가 어려웠다. 3)Iodine의 착색대와 비착색대의 비율에 의한 역가의 정성적인 측정이 가능하였다.

• 농촌의학ㆍ지역보건
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• 제22권1호
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• pp.61-74
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• 1997

In case of tissue invading nematode, proteolytic enzyme was required at their parasitic life. Proteinases obtained from these parasites(Toxocara canis, Ansakis spp. and Trichinella spiralis) were extracted, isolated and further purified. And then the analysis for activity and inhibitory effect of proteinases were performed by appropriate substrate. Determination of protein as a circulating antigen was done in use of infected animal serum with above parasites, respectively. For above experimental objects, following procedures were performed. First, enzymatic activity was measured in use of azocasein and inhibitory effect of porteinase were studied by various inhibitors. Second, partially purified proteins containing enzymatic activity were obtained by ion exchange chromatography, ultrafiltration and electrophoretic elution. Third, role of the partially purified protein as a circulating antigen. The results obtained were as follows : 1. Enzymatic activity of each nematode proteinase was varied according to pH. Optimal pH of Toxocara canis, Ansakis spp. and Trichinella spiralis were pH 6.0, pH 5.5 and pH 6.5, respectively. The optimal molarity of buffer was 0.1M phosphate buffer. Although little difference between these proteinases was observed, temperature stability was at least maintained at $4^{\circ}C$ until 5 days. 2. In case of Ansakis spp. and Toxocara canis, enzymatic activity of these proteinases was considerably inhibited by Leupeptin and EDTA. For maximum enzymatic activity of above proteinases, it was required that cysteine residue of enzyme should be protected. And it was suggested that metallo type was contained in enzyme active site. Proteinase of Trichinella spiralis contained metallo type also. 3. Although partial purification was performed in Ansakis spp. and Toxocara canis, proteins maintaining enzymatic activity were identified as a circulating antigen. From SDS-PAGE and immunoblot, 25 kDa was presented in Ansakis spp.. Specific antigen of Toxocara cains was 110 kDa protein fraction. 55 and 42 kDa proteins were reacted with normal serum. Trichinella spiralis 60 kDa protein fraction was successfully purified from excretory materials in culture. As a result of immune-reaction with Trichinella spiralis infected serum, highly purified 60 kDa protein was maintained antigenicity until final purification step.

• 한국토양비료학회지
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• 제45권4호
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• pp.560-564
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• 2012

최근에 건강식품의 선호에 따라 신선 채소류 등의 소비가 증가하면서 농산물의 안전성에 대한 관심이 점점 증가하고 있다. 이에 본 연구는 국내 유통 중인 유기농 채소류를 구입하여 총균수, Salmonella spp., E. coli O157:H7, S. aureus, L. monocytogenes, Y. enterocolitica 등의 병원성 미생물의 오염도를 분석하고 유기농산물의 식중독균에 대한 잠재적인 위험성을 평가하는 기초자료에 도움을 주고자 수행하였다. 유기농 채소류 4종의 일반 세균수 수준을 비교한 결과 오이의 일반 세균수 수준이 가장 높았고, 토마토의 일반 세균수는 가장 낮았다. 유기농 채소 중 깻잎의 일반 세균수는 $4.2{\sim}7.7log\;CFU\;g^{-1}$, 상추는 $5.0{\sim}8.0log\;CFU\;g^{-1}$, 토마토는 $4.0{\sim}7.5log\;CFU\;g^{-1}$, 오이는 $6.6{\sim}8.6log\;CFU\;g^{-1}$ 범위였다. 유기농 채소에서 Salmonella spp., E. coli O157:H7, S. aureus, L. monocytogenes, Y. enterocolitica 등은 검출되지 않았다. 일반 세균수는 관행 농산물과 비슷한 수준을 보였으며 병원성 미생물은 전혀 검출되지 않았기 때문에 식중독균 오염수준은 극히 낮을 것이라고 생각한다. 그런데 오염될 수 있는 가능성이 있으므로 생산, 유통 단계에서 주의 깊은 관리가 필요하다고 판단된다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권1호
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• pp.37-44
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• 2016

Several infectious agents are considered to be causes of cancer in human, mainly hepatitis B and C viruses, high-risk human pailloma viruses, Helicobacter pylori, Clonorchis sinensis, and Opisthorchis viverrini. Here we described the evident research and the association between Helicobacter spp. and biliary tract cancer particularly cholangiocarcinoma (CCA). Global epidemiological studies have suggested that Helicobacter spp. are possible risk factors for biliary tract diseases. Molecular studies support a linkage of Helicobacter spp. with CCA development. H. pylori, H. bilis, and H. hepaticus, are found in CCA, but the most common species are H. pylori and H. bilis. The type of CCA are associated with Helicobacter spp. include extrahepatic CCA, and common bile duct cancer. Up to the present, however, the results from different regions, materials and methods, sub-sites of cancer, and controls have not been consistent, thus introducing heterogeneity. Therefore, a comparison between co-Helicobacter spp.-CCA in the countries with low and high incident of CCA is required to settle the question. Furthermore, clarifying variation in the role of Helicobacter species in this CCA, including pathogenesis of CCA through enhanced biliary cell inflammation and proliferation, is necessary.