• 대한수학회보
• /
• 제55권5호
• /
• pp.1419-1431
• /
• 2018

Let $f=h+{\bar{g}}$ be a harmonic mapping of the unit disk ${\mathbb{D}}$ with the holomorphic part h satisfying that h is injective and $h({\mathbb{D}})$ is an M-linearly connected domain. In this paper, we obtain the sufficient and necessary conditions for f to be bi-Lipschitz, which is in particular, quasiconformal. Moreover, some distortion theorems are also obtained.

• East Asian mathematical journal
• /
• 제25권2호
• /
• pp.221-227
• /
• 2009

In this paper, we introduce the generalized H$\ddot{o}$lder space with a majorant function and prove the H$\ddot{o}$lder regularity for solutions of the Cauchy-Riemann equation in the generalized Holder spaces on a bounded convex domain in $\mathbb{C}^2$.

• 한국자기공명학회논문지
• /
• 제12권1호
• /
• pp.1-13
• /
• 2008

Acetyl-CoA carboxylase (ACC) catalyzes the first step in fatty acid biosynthesis: the synthesis of malonyl-CoA from acetyl-CoA. As essential regulators of fatty acid biosynthesis and metabolism, ACCs are regarded as therapeutic targets for the treatment of metabolic diseases such as obesity, In ACC, the biotinoyl domain performs a critical function by transferring an activated carboxyl group from the biotin carboxylase domain to the carboxyl transferase domain, followed by carboxyl transfer to malonyl-CoA. Despite the intensive research on this enzyme, only the bacterial and yeast ACC structures are currently available, To explore the mechanism of ACC holoenzyme function, we determined the structure of the biotinoyl domain of human ACC2 and analyze its characteristics using NMR spectroscopy. The 3D structure of the hACC2 biotinoyl domain has a similar folding topology to the previously determined domains from E. coli and P. Shermanii, however, the 'thumb' structure is absent in the hACC2 biotinoyl domain. Observations of the NMR signals upon the biotinylation indicate that the biotin group of hACC2 does not affect the structure of the biotinoyl domain, while the biotin group for E. coli ACC interacts directly with the thumb residues that are not present in the hACC2 structure. These results imply that, in the E. coli ACC reaction, the biotin moiety carrying the carboxyl group from BC to CT can pause at the thumb of the BCCP domain. The human biotinoyl domain, however, lacks the thumb structure and does not have additional non-covalent interactions with the biotin moiety; thus, the flexible motion of the biotinylated lysine residue must underlie the "swinging arm" motion. This study provides insight into the mechanism of ACC holoenzyme function and supports the "swinging arm" model in human ACCs.

• 생명과학회지
• /
• 제17권3호통권83호
• /
• pp.345-349
• /
• 2007

PDZ 도메인을 통하여 여러 단백질과 상호작용하며 신경전달 기전에 관여하는 단백질로 Shank1, Shank2, Shank3, PDS-95, AF있다. 본 연구는 Shank3 PDZ 도메인의 구조를 밝히기 위한 첫 단계로서 Shank3 단백질의 PDZ 도메인을 동정하였고, E. coli에서 발현하여 생성된 단백질을 정제한 후 1차 NMR 구조분석을 시도하였다. 그 결과에 의하면 정제된 Shank3 PDZ 단백질은 순도가 높고 안정적인 접힘(folding)구조를 제시하고있다. 현재 완전한 NMR 구조분석을 위해 좀더 많은 양의 정제된 Shank3 PDZ 단백질을 얻고자 연구하고 있다.

• 대한전자공학회논문지SD
• /
• 제44권3호
• /
• pp.20-25
• /
• 2007

본 논문에서는 $LiNbO_3$의 선택적 영역을 도메인 반전을 수행하였으며, 이를 대역변조기 및 SSB 광변조기 제작에 응용하였다. 인가전압에 대한 회로적인 응답전류를 분석 및 고려함으로써 도메인 벽의 이동속도를 정확히 제어할 수 있었다. 과도한 도메인의 벽 이동속도에 의한 도메인 반전 형상을 확인하였고, 또한 도메인 벽의 진행방향에 따라 그 속도의 차이가 발생함을 알 수 있었다. 제작된 대역변조기는 30.3 GHz를 중심주파수로 하여 5.1GHz의 3dB 대역폭을 보였고. SSB 광변조기의 변조 스펙트럼으로부터 19dBm의 5.8GHz RF 입력신호에 대해 USB가 LSB에 비해 33dB정도 억제됨을 확인할 수 있었다.

• Ocean Systems Engineering
• /
• 제4권2호
• /
• pp.99-115
• /
• 2014

When caissons are mounted on a floating transportation barge and towed by a tug boat in waves, motion of the floating dock creates inertia and gravity-induced slip forces on the caisson. If its magnitude exceeds the corresponding friction force between the two surfaces, a slip may occur, which can lead to an unwanted accident. In oblique waves, both pitch and roll motions occur simultaneously and their coupling effects for slip and friction forces become more complicated. With the presence of strong winds, the slip force can appreciably be increased to make the situation worse. In this regard, the safety of the transportation process of a caisson mounted on a floating dock for various wind-wave conditions is investigated. The analysis is done by both frequency-domain approach and time-domain approach, and their differences as well as pros and cons are discussed. It is seen that the time-domain approach is more direct and accurate and can include nonlinear contributions as well as viscous effects, which are typically neglected in the linear frequency-domain approach.

• Journal of Microbiology and Biotechnology
• /
• 제17권10호
• /
• pp.1607-1615
• /
• 2007

We investigated the applicability of the TEM-l ${\beta}$-lactamase fragment complementation (BFC) system to develop a strategy for the screening of protein-protein interactions in bacteria. A BFC system containing a human Fas-associated death domain (hFADD) and human Fas death domain (hFasDD) was generated. The hFADD-hFasDD interaction was verified by cell survivability in ampicillin-containing medium and the colorimetric change of nitrocefin. It was also confirmed by His pull-down assay using cell lysates obtained in selection steps. A coiled-coil helix coiled-coil domain-containing protein 5 (CHCH5) was identified as an interacting protein of human uracil DNA glycosylase (hUNG) from the bacterial BFC cDNA library strategy. The interaction between hUNG and CHCH5 was further confirmed with immunoprecipitation using a mammalian expression system. CHCH5 enhanced the DNA glycosylase activity of hUNG to remove uracil from DNA duplexes containing a U/G mismatch pair. These results suggest that the bacterial BFC cDNA library strategy can be effectively used to identify interacting protein pairs.

• 한국자기공명학회논문지
• /
• 제11권1호
• /
• pp.24-29
• /
• 2007

[ ${\beta}ig-h3$ ] is an extracellular matrix protein that mediates cell adhesion through interaction with integrins. The 18 residue YH motifs within each fas-1 domain are known to be responsible for the interaction with the ${\alpha}_v{\beta}_5$ integrin, and the synthetic YH motif peptides are known to inhibit endothelial tube formation and reduces the number of blood vessels, and so expected to be an effective inhibitor of angiogenesis. In this study, we solved the 3D structure of the 18 residue YH motif peptide (EALRDLLNNHILKSAMCA; D2 peptide) within the second fas-1 domain of ${\beta}ig-h3$ using NMR. The Peptide has ${\alpha}-helix$ structure at the C terminal region but the N terminal region is flexible. The present structural information may be helpful for developing more effective peptide drug candidate for the treatment of diseases dependent on angiogenesis.

• BMB Reports
• /
• 제50권5호
• /
• pp.269-274
• /
• 2017

The biological activities of macrophage migration inhibitory factor (MIF) might be mediated through a classical receptor-mediated or non-classical endocytic pathway. JAB1 (C-Jun activation domain-binding protein-1) promotes the degradation of the tumor suppressor, p53, and the cyclin-dependent kinase inhibitor, p27. When MIF and JAB1 are bound to each other in various intracellular sites, MIF inhibits the positive regulatory effects of JAB1 on the activity of AP-1. The intestinal parasite, Anisakis simplex, has an immunomodulatory effect. The molecular mechanism of action of As-MIF and human JAB1 are poorly understood. In this study, As-MIF and hJAB1 were expressed and purified with high solubility. The structure of As-MIF and hJAB1 interaction was modeled by homology modeling based on the structure of Ace-MIF. This study provides evidence indicating that the MIF domain of As-MIF interacts directly with the MPN domain of hJAB1, and four structure-based mutants of As-MIF and hJAB1 disrupt the As-MIF-hJAB1 interaction.

• 방송공학회논문지
• /
• 제21권4호
• /
• pp.592-608
• /
• 2016

최근 거의 완벽한 3차원 영상정보를 갖고 있는 디지털 홀로그램에 대한 연구가 활발히 이루어지고 있어 조만간 디지털 홀로그램의 서비스가 시작될 것으로 보인다. 따라서 본 논문에서는 이 서비스에 사용될 수 있는 디지털 홀로그램 동영상을 압축하는 기술을 제안한다. 이 방법은 가장 최근에 국제표준으로 제정된 2차원 동영상 압축 기술인 H.265/HEVC를 사용하는데, 디지털 홀로그램의 화소 간 상관성을 높이기 위하여 도메인 변환 방법을 사용한다. 또한 압축효율을 높이기 위해 H.265/HEVC의 다양한 압축 파라미터들을 고려한다. 본 논문의 목적은 다양한 도메인 변환 방법, 도메인 변환 단위, 압축 파라미터들 중 가장 압축효율이 좋은 최적의 압축조건을 실험을 통하여 구하는 것이다. 이렇게 구해진 조건을 만족하는 압축방법을 기존의 방법들과 비교하여 본 논문에서 제시하는 방법의 우수성을 보인다.