• 한국자기공명학회논문지
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• 제7권1호
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• pp.37-45
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• 2003

Human CD99 is a ubiquitous 32-kDa transmembrane protein encoded by mic2 gene. Recently it has been reported that expression of a splice variant of CD99 transmembrane protein (Type I and Type II) increases invasive ability of human breast cancer cells. To understand structural basis for cellular functions of CD99 Type II, we have initiated studies on hCD99$\^$TMcytoI/ using circular dichroism (CD) and multi-dimensional NMR spectroscopy. CD spectrum of hCD99$\^$TMytoI/ in the presence of 200mM DPC and CHAPS displayed an existence ${\alpha}$-helical conformation, showing that it could form an ${\alpha}$-helix under membrane environments. In addition, we have found that the cytoplasmic domain of CD99 would form symmetric dimmer in the presence of transmembrane domain. Although it has been rarely figured out the correlation between structure and functional mechanism of hCD99$\^$TMcytoI/, the dimerization or oligomerization would play an important role in its biological function.

• Journal of Microbiology and Biotechnology
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• 제25권1호
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• pp.119-126
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• 2015

Among the various human growth factors, epidermal growth factor (hEGF, consisting of 53 amino acids) has various effects on cell regeneration, stimulation of proliferation, migration of keratinocytes, formation of granulation tissues, and stimulation of fibroblast motility, which are important for wound healing. Owing to their multiple activities, EGFs are used as pharmaceutical and cosmetic agents. However, their low productivity, limited target specificity, and short half-life inhibit their application as therapeutic agents. To overcome these obstacles, we fused the collagen-binding domain (CBD) of Vibrio mimicus metalloprotease to EGF protein. About 18 or 12 amino acids (aa) (of the 33 total amino acids), which were essential for collagen-binding activity, were combined with the N- and C-termini of EGF. We constructed, expressed, and purified EGF (53 aa)-CBD (18 aa), EGF (53 aa)-CBD (12 aa), CBD (18 aa)-EGF (53 aa), and CBD (12 aa)-EGF (53 aa). These purified recombinant proteins increased the numbers of cells in treated specimens compared with non-treated specimens and control hEGF samples. The collagen-binding activities were also evaluated. Furthermore, CBD-hybridized hEGF induced phosphorylation of the EGF receptor. These results suggested that these fusion proteins could be applicable as small therapeutic agents in wound tissue healing.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1997년도 추계학술대회
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• pp.55-58
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• 1997

In this paper, we study a signal processing method which extracts each MUAP(motor unit action potential) from EMG(Electromyogram) interference pattern or clinical diagnostic purposes. First of all, differential digital filtering is selected or eliminating the spike components of the MUAP's from the background noise. And, the algorithm identifies the spikes over the certanin threshold by template matching in frequency domain. After missing or false firing actor is cut off at the IPI(inter pulse interval) histogram, we averages the MUAP waveforms from the raw signal using the identified spikes as triggers, and Finally, measures their amplitudes, durations, and numbers of phases. Specially, We introduce algorithm performed by template matching in the frequency domain. A typical 3-s signal recorded from the biceps brachii muscle using a conventional needle electrode during a isometric contraction is used. Finally, the method decomposed five simultaneous active MUAP's from original EMG signal.

• 대한전자공학회논문지SP
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• 제45권6호
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• pp.57-63
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• 2008

본 논문에서는 웨이블릿 영역에서 이변수 가우스 확률밀도함수를 이용하여 잡음을 효과적으로 제거하는 방법을 제안한다. 본 논문의 방법은 웨이블릿 영역의 스케일간의 관계에 대한 통계적 모델을 이변수 가우스 확률분포로 설정하고, 이에 대한 베이즈 추정법을 통하여 잡음 제거를 수행한다. 베이즈 추정법을 위한 통계 파라메터는 $H{\ddot{o}}lder$ 부등식을 이용하여 근사적으로 추정한다. 실험 결과를 통하여 본 논문의 방법이 기존의 이변수 사전 확률모델을 이용한 잡음 제거 방법에 비하여 우수한 결과를 보여 준다는 것을 알 수 있다.

• BMB Reports
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• 제56권3호
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• pp.172-177
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• 2023

BEST family is a class of Ca²⁺-activated Cl- channels evolutionary well conserved from bacteria to human. The human BEST paralogs (BEST1-BEST4) share significant amino acid sequence homology in the N-terminal region, which forms the transmembrane helicases and contains the direct calcium-binding site, Ca²⁺-clasp. But the cytosolic C-terminal region is less conserved in the paralogs. Interestingly, this domain-specific sequence conservation is also found in the BEST1 orthologs. However, the functional role of the C-terminal region in the BEST channels is still poorly understood. Thus, we aimed to understand the functional role of the C-terminal region in the human and mouse BEST1 channels by using electrophysiological recordings. We found that the calcium-dependent activation of BEST1 channels can be modulated by the C-terminal region. The C-terminal deletion hBEST1 reduced the Ca²⁺-dependent current activation and the hBEST1-mBEST1 chimera showed a significantly reduced calcium sensitivity to hBEST1 in the HEK293 cells. And the C-terminal domain could regulate cellular expression and plasma membrane targeting of BEST1 channels. Our results can provide a basis for understanding the C-terminal roles in the structure-function of BEST family proteins.

• 정보과학회 논문지
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• 제42권2호
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• pp.162-168
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• 2015

대부분의 영상정보들은 데이터 양이 방대하기 때문에 압축되어 저장되고 전달된다. 이 때문에 영상정보의 변환을 위해서는 사전에 압축을 해제하여 공간정보를 추출한 뒤에 소정의 변환을 하고 다시 압축해야 하는 번거로움과 불편한 점이 있다. 본 논문에서는, JPEG등의 정지영상 압축이나 MPEG-4와 H.264 등의 영상 압축 표준에서 널리 사용되는 DCT 변환에 의한 영상정보의 주파수 영역의 계수 값들의 부호나 이들 간의 상대적 위치를 적절히 바꿈으로써 공간적 영역에서의 원하는 기하학적 변환이 이루어지는 규칙을 발견하고 정리하였다. 본 저자들은 주파수 영역에서의 계수 값 변환 효과가 공간적 영역에 어떻게 나타나는지 수학적으로 유도하고 실제 JPEG 압축 파일에 본 방식을 적용하여 이미지를 변환시켜 그 성능을 검증하였다. 이 방법은 압축을 완전히 해제하지 않고도 공간적 영역에서의 변환을 달성할 수 있기 때문에 대용량의 간단한 이미지 암호화를 위한 경량암호화 시스템에 응용될 수 있다.

• Molecules and Cells
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• 제37권10호
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• pp.753-758
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• 2014

Sorting nexin 9 (SNX9) is a member of the sorting nexin family of proteins and plays a critical role in clathrinmediated endocytosis. It has a Bin-Amphiphysin-Rvs (BAR) domain which can form a crescent-shaped homodimer structure that induces deformation of the plasma membrane. While other BAR-domain containing proteins such as amphiphysin and endophilin have an amphiphatic helix in front of the BAR domain which plays a critical role in membrane penetration, SNX9 does not. Thus, whether and how SNX9 BAR domain could induce the deformation of the plasma membrane is not clear. The present study identified the internal putative amphiphatic stretch in the $1^{st}$ ${\alpha}$-helix of the SNX9 BAR domain and proved that together with the N-terminal helix ($H_0$) region, this internal putative amphiphatic stretch is critical for inducing membrane tubulation. Therefore, our study shows that SNX9 uses a unique mechanism to induce the tubulation of the plasma membrane which mediates proper membrane deformation during clathrinmediated endocytosis.

• Journal of Microbiology and Biotechnology
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• 제18권6호
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• pp.1186-1190
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• 2008

Single-chain Fv (scFv) antibody against c-Met is expected to be employed in clinical treatment or imaging of cancer cells owing to the important biological roles of c-Met in the proliferation of malignancies. Here, we show that the productivity of scFv against c-Met in Escherichia coli is significantly influenced by the orientation of its variable domains. We generated anti-c-Met scFv antibodies with two different domain orders (i.e., $V_L$-linker-$V_H$ and $V_H$-linker-$V_L$), expressed them in the cytoplasm of E. coli trx/gor deleted mutant, and compared their specific activities as well as their productivities. Productivity of total and functional anti-c-Met scFv with $V_H/V_L$ orientation was more than five times higher than that with $V_L/V_H$ format. Coexpression of DsbC enhanced the yield of soluble amounts of anti-c-Met scFv protein for both constructs. The purified scFv antibodies of the two different formats exhibited almost the same antigen-binding activities. We also compared the productivities and specific activities of anti-c-Met diabodies with $V_H/V_L$ or $V_L/V_H$ formats and obtained similar results to the case of scFv antibodies.

• 한국통신학회논문지
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• 제34권10C호
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• pp.975-982
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• 2009

본 논문에서는 저연산, 저지연성을 보장하는 다중 비디오 스트림 흔합 알고리즘을 제안한다. 제안된 알고리즘은 슬라이스기반 H.264/AVC 비디오 스트림들을 화소 도메인에서 혼합하지 않고 압축 도메인에서 신택스 비트스트림 변경에 의해 혼합한다. 혼합을 위한 신택스 비트스트림 변환은 혼합화면을 구성할 비디오 스트림들의 위치에 따라 변경한다. 실험결과 4개 이상의 비디오 스트림들을 고속으로 혼합할 수 있었다. 또한 표준 H.264/AVC를 지원하는 플레이어에서 화질열화 없이 재생할 수 있었다.

• 대한기계학회논문집A
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• 제26권2호
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• pp.399-405
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• 2002

This paper proposes a new type of piezoactuator-driven valve system. The piezoceramic actuator bonded to both sides of a flexible beam surface makes a movement required to control the pressure at the flapper-nozzle of a pneumatic valve system. After establishing a dynamic model, an appropriate size of the valve system is designed and manufactured. Subsequently, a robust H$_{\infty}$ control algorithm is formulated in order to achieve accurate tracking control of the desired pressure. The controller is experimentally realized and control performance for the sinusoidal pressure trajectory is presented in time domain. The control bandwidth of the valve system, which directly represents the fastness, is also evaluated in the frequency domain.