• 한국수정란이식학회지
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• 제24권3호
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• pp.199-205
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• 2009

This study was carried out to investigate the effective genetic resources preservation system using the frozen boar semen. The porcine oocytes were matured for 44 hours in NCSU-23 medium with or without 10% Porcine Follicle Fluid (PFF), 0.5 ${\mu}g/ml$ porcine FSH, 0.5 ${\mu}g/ml$ equine LH, 1.0 ${\mu}g/ml$ 17 $\beta$-estradiol ($E_2$) and 10 ng/ml Epidermal Growth Factor (EGF) under mineral oil at $38.5^{\circ}C$ in humidified atmosphere of 5% $CO_2$ in air. After 44 h of culture, the oocytes were inseminated with frozen-thawed semen and fresh semen prepared with mTBM medium for 6 h. Later, set of 50 presumptive zygotes were transferred into 4-well dish (500 ${\mu}l$) of IVC medium. for embryos freezing, slow-freezing and vitrification methods were used as a cryopreservation. Differences among treatments were analyzed using General Linear Model Procedure by SAS Package (version 6.12) differences were considered significant when p<0.05. Following IVF and IVC, the rates of cleavage and blastocysts formation were significantly higher (p<0.05) in hormone supplemented group than that of hormone-free group (25.7 vs, 12.1). The development rates to cleavage and blastocysts were significantly higher in PZM-5 group than NCSU-23 group (60.3%, 46.6% vs 27.4%, 11.1%). Further improvement was achieved when PZM-5 was supplemented with FBS. Cleavage rates was significantly higher in fresh semen source group than frozen semen (66.7% vs 43.7%). However in blastocysts rates was similar two groups. Post-thaw survival rates of embryos were 1.2% and 2.2% in slow-frezing and vitrification groups, respectively. The results of our study suggest that it is still possible to improve the culture conditions and boar semen cryopreservation for enhance reproductive technology and animal genetic resources conservation.

• 한국식품저장유통학회지
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• 제15권6호
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• pp.832-839
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• 2008

스톡은 수프와 소스를 만들기 위한 첫 과정으로 수프, 소스 및 주 요리의 맛을 좌우한다. 본 연구에서는 단호박만을 재료로 한 스톡 제조를 위하여 단호박의 첨가량, 물의 첨가량 및 $97^{\circ}C$에서의 가열시간 등의 3요인을 독립변수로, 단호박 스톡의 색상, 향, 맛 및 종합적기호도의 4가지 관능적 특성을 종속변수로 하여 반응표면분석으로 단호박 스톡 제조조건을 최적화하고, 최적 조건에서 제조한 단호박 스톡의 품질 특성을 조사하였다. 그 결과 단호박 스톡의 최적 제조조건은 단호박의 첨가량 $357.9{\sim}403.0\;g$, 물의 첨가량 $689.8{\sim}768.5\;mL$ 및 $97^{\circ}C$에서의 가열시간 $9.9{\sim}10.3$분이었다. 단호박의 첨가량 380.5 g, 물의 첨가량 729.2 mL 및 $97^{\circ}C$에서의 가열시간 10.1분의 조건에서 제조한 단호박 스톡의 pH는 6.64, 산도는 0.18%, 당도는 $2.39\;^{\circ}Brix$이었으며, 색도는 L*값 99.07, a*값 -2.43 및 b*값 11.82이었다. 또한 항산화능을 조사한 결과, 단호박 스톡의 총 폴리페놀은 280.75 mg/L, 전자공여능은 21.32% 이었다.

• 대한치과기공학회지
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• 제35권2호
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• pp.157-171
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• 2013

Purpose: This study aims to present a database for the development of a healthcare management program based on the survey and analysis of self reported psychosomatic symptoms among the current dental technology students. Methods: Subjects of our study are 480 dental technology major students enrolled in a third year college located Gyeonggi, Chungcheong, and Gangwon province. Using a random sampling, we conducted a self-report survey from August 30, 2011 to October 28, 2011 and 418 reports were collected as feedback and we put an analysis on them. Results: 1. The average physical self symptom was 20.49, which is higher than the average mental self symptom(18.54). Of the subcategories of psychosomatic self symptom, we observed multiple subjective symptoms as the highest one(37.77), and aggression as the lowest(13.77). 2. As to gender, both physical and mental self symptom were statistically significant with the scale score of(p<.001). The scale score of subcategories is as follows; multiple subjective symptoms(I, p<.001), eye and skin(B, p<.001), digestive(C, p<.001), impulsiveness(H, p<.001), lie scale(L, p<.001), mental instability(J, p<.001), depression(K, p<.001), aggression(F, p<.001), irregularity of life(G, p<.001), mouth and anal(D, p<.05), nervousness(E, p<.05). 3. As for obesity, statistical significance was shown with the scale scores of physical self symptom(p<.001), multiple subjective symptoms(I, p<.001), digestive(C, p<.001), aggression(F, p<.001), depression(K, p<.01), irregularity of life(G, p<.01), respiratory(A, p<.05), eye and skin(B, p<.05), impulsiveness(H, p<.05), mental instability(J, p<.05). The scale scores in the environmental quality and life satisfaction were shown as follows; depression(K, p<.001), lie scale(L, p<.01), and irregularity of life(G, p<.05). 4. We employed multiple regression analysis to take account of general factors affecting psychosomatic self symptoms, and drew that the explanatory power of the model was proved with the scales of physical self symptom(4.1%) and mental self-symptom(3.6%). Obesity was a factor that affects physical self symptom with the scale score of(p<.01), and environmental quality and life satisfaction(p<.01) and obesity(p<.05) affect mental self symptom. Conclusion: In this analysis we observed obesity of dental technology students can influence their psychosomatic self symptoms. In this sense, it would be reasonable to develop a healthcare management and education programs that help the students maintain a healthy weight and promote their health.

• Journal of Animal Science and Technology
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• 제49권4호
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• pp.443-450
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• 2007

본 연구에서는 엘크 사슴의 계절별 정액성상 변화, 동결정액의 융해 후 활력 및 발정동기화 후 인공수정 시간이 수태율에 미치는 영향을 조사하여 효율적인 인공수정 방법을 제시코자 시도하였다. 수사슴에서 정액의 연중 변화는 정액량, 정자 농도, 총 정자 수 및 정자 활력은 7월부터 급격히 증가 되어 정액량과 총 정자 수 및 정자 운동성은 10월에 최고치에 달했고, 정자 농도는 9월에 가장 많은 것으로 조사 되었다. 그 후 정액량, 정자농도, 총 정자 수 및 정자 운동성은 점진적으로 감소하여 정액 양, 정자 농도는 4~6월, 총 정자 수는 4~5월 및 정자 운동성은 6월에 최저 수준으로 감소하였다.번식계절(9~2월)과 비번식계절(3~8월)의 정자 농도, 총 정자 수 및 정자 운동성은 번식계절이 유의적으로 높게 조사되었다(P<0.05). 번식계절과 비번식계절에 채취한 정액의 동결과정 중에 정자 운동성의 결과는 채취 직후, 5℃냉각 후 및 동결 융해 후 활력이 번식계절이 높은 것으로 조사 되었다(P<0.05).인공수정 시간에 따른 수태율은 CIDR 제거 후 60시간에 인공수정 한 처리구가 수태율이 다른 시간들 보다 다소 높았으나 유의적인 차이는 나타나지 않았다.이러한 연구 결과는 엘크 사슴에서 정액 생산과 인공수정을 수행함에 있어 유용한 자료로 이용 될 수 있으며, 발정동기화 후 호르몬 변화, 배란 및 수정 적기 등에 관한 연구가 추가적으로 수행되어야 할 것으로 사료된다.

• Clinical and Experimental Reproductive Medicine
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• 제26권1호
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• pp.9-20
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• 1999

The genetic defects in human gametes and embryos can cause adverse effects on overall reproductive events. Biopsy of embryos for preimplantation genetic diagnosis (PGD) offers a new possibility of having children free of the genetic disease. In addition, advanced embryo culture method may enhance the effectiveness of embryo biopsy for the practical application of PGD. This experimental study was undertaken to evaluate the effects of coculture on the development in vitro of biopsied mouse embryos as a preclinical model for PGD of human embryos. Embryos were obtained after in vitro fertilization (IVF) from F1 hybrid mice (C57BLfemale/CBAmale). Using micromanipulation, 1, 2, 3 or 4 blastomeres of 8-cell stage embryos were aspirated through a hole made in the zona pellucida by zona drilling (ZD) with acidic Tyrode's solution (ATS). After biopsy of blastomeres, embryos were cultured in vitro for 110 hours in Ham's F-10 supplemented with 0.4% BSA or cocultured on the monolayer of Vero cells in the same medium. The frequence of blastocyst formation were recorded, and the embryos beyond blastocyst stage were stained with 10% Giemsa to count the total number of nuclei in each embryo. There was no significant difference in the blastocyst formation between the zona intact control group and the zona drilling (ZD) only, or biopsied groups. The hatching rate of all the treatment groups except 4/8 group was significantly higher than that of control group. In all the treatment groups, there was a significant reduction in the mean cell number of embryos beyond blastocyst stage ($50.2{\pm}14.0$ in control group vs. $41.2{\pm}7.9$ in ZD, $39.3{\pm}8.8$ in 7/8, $29.7{\pm}6.4$ in 6/8, $25.1{\pm}5.7$ in 5/8, and $22.1{\pm}4.3$ in 4/8 groups, p<0.05). When the same treatments were followed by coculture with Vero cells, a similar pattern was seen in the blastocyst formation and the hatching rate. However, in all the treatment groups, there was a significant increase in the mean cell number of embryos beyond blastocyst stage with coculture, compared with the parallel groups without coculture. In the cleavage rate of biopsied blastomeres cultured for 110 hours after IVF, there was no significant difference between coculture and non-coculture groups (87.2% vs. 78.7%). However, the mean cell number of embryos developed from the biopsied blastomeres was significantly higher in coculture group ($11.5{\pm}4.7\;vs.\;5.9{\pm}1.9$, p<0.05). In conclusion, biopsy of mouse embryos after ZD with ATS is a safe and highly efficient method for PGD, and coculture with Vero cells showed a positive effect on the development in vitro of biopsied mouse embryos and blastomeres as a preclinical model for PGD of human embryos.

• 한국입자에어로졸학회지
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• 제7권2호
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• pp.59-68
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• 2011

The objective of the study was to investigate the main factors that contribute the variation of $PM_{10}$ concentration of Seoul and to quantify their effects using generalized additive model (GAM). The analysis was performed with 3 year air pollution data (2004~2006) measured at 27 urban sites and 7 roadside sites in Seoul, a background site in Gangwha and a rural site in Pocheon. The diurnal variation of urban $PM_{10}$ concentrations of Seoul showed a typical bimodal pattern with the same peak times as that of roadside, and the maximum difference of $PM_{10}$ level between urban and roadside was about $14{\mu}g/m^{3}$ at 10 in the morning. The wind direction was found to be a major factor that affects $PM_{10}$ level in all investigated areas. The overall $PM_{10}$ level was reduced when air came from east, but background $PM_{10}$ level in Gangwha was rather higher than the urban $PM_{10}$ level in Seoul, indicating that the $PM_{10}$ level in Gangwha is considerably influenced by that in Seoul metropolitan area. When hourly variations of $PM_{10}$ were analyzed using GAM, wind direction and speed explained about 34% of the variance in the model where the variables were added as a 2-dimensional smoothing function. In addition, other variables, such as diurnal variation, difference of concentrations between roadside and urban area, precipitation, month, and the regression slope of a plot of carbon monooxide versus $PM_{10}$, were found to be major explanatory variables, explaining about 64% of total variance of hourly variations of $PM_{10}$ in Seoul.

• Smart Structures and Systems
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• 제20권1호
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• pp.85-97
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• 2017

The aim of this research is to develop a new method to use magnetorheological (MR) damper for vibration control. It is a new way to achieve the MR damper response without the need to have detailed constant parameters estimations. The methodology adopted in designing the control structure in this work is based on the experimental results. In order to investigate and understand the behaviour of an MR damper, an experiment is first conducted. Force-displacement and force-velocity responses with varying current have been established to model the MR damper. The force for upward and downward motions of the damper piston is found to be increasing with current and velocity. In cyclic motion, which is the combination of upward and downward motions of the piston, the force with hysteresis behaviour is seen to be increasing with current. In addition, the energy dissipated is also found to be linear with current. A proportional-integral-derivative (PID) controller, based on the established characteristics for a quarter car suspension model, has been adapted in this study. A fuzzy rule based PID controller (F-PID) is opted to achieve better response for a varying frequency input. The outcome of this study can be used in the modelling of MR damper and applied to control engineering. Moreover, the identified behaviour can help in further development of the MR damper technology.

• 한국전산유체공학회지
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• 제21권2호
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• pp.70-80
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• 2016

The comparison of two commercial codes(FLUENT and STAR-CCM+) and an open-source code(OpenFOAM) are carried out for the aerodynamic analysis of flight vehicles at low speeds. Tailless blended-wing-body UCAV, main wing and propeller of HALE UAV(EAV-3) are chosen as geometries for the investigation. Using the same mesh, incompressible flow simulations are carried out and the results from three different codes are compared. In the linear region, the maximum difference of lift and drag coefficients of UCAV are found to be less than 2% and 5 counts, respectively and shows good agreement with wind tunnel test data. In a stall region, however, the reliability of RANS simulation is found to become poor and the uncertainty according to code also increases. The effect of turbulence models and meshes generated from different tools are also examined. The transition model yields better results in terms of drag which are much closer to the test data. The pitching moment is confirmed to be sensitive to the existence and the location of transition. For the case of EAV-3 wing, the difference of results with ${\kappa}-{\omega}$ SST model is increased when Reynolds number becomes low. The results for the propeller show good agreement within 1% difference of thrust. The reliability and uncertainty of three codes is found to be reasonable for the purpose of engineering use. However, the physical validity and reliability of results seem to be carefully examined when ${\kappa}-{\omega}$ SST model is used for aerodynamic simulation at low speeds or low Reynolds number conditions.

• Membrane and Water Treatment
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• 제11권2호
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• pp.123-130
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• 2020

Submerged Membrane bioreactor (SMBR) is one of the last techniques that allow a high quality of treated industrial effluents by coupling biological treatment and membrane separation. Thus, this research was an effort to evaluate performance of a SMBR treating a model textile wastewater (MTWW). Different SMBR operating parameters like mixed liquor suspended solids (MLSS) and Dissolved oxygen concentration, hydraulic retention time (HRT), and nutrients addition (N and P) have been investigated. MTWW (influent to the SMBR) was generated using the reactive azo-dye, Novacron blue FNG (100mg/L feed concentration). Results of MTWW treatment using SMBR under optimal operating conditions (MLSS, 4.2-13.3g/L; HRT, 4 days; pH, 6.9-7.2; conductivity, 400-900 μS/cm and temperature, 19.4-22.2 ℃) showed that COD and blue colour treatment performances are between 94-98% and 30-80%, respectively. It is concluded that SMBR can be used in large scale textile wastewater treatment plants to improve effluent quality in order to meet effluent discharge standards.

• International Journal of CAD/CAM
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• 제6권1호
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• pp.9-17
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• 2006

Performing inspections for Hull Condition Monitoring and Assessment as stipulated in IACS unified requirements and IMO's Condition Assessment Scheme (CAS) IMO Resolution MEPC.94(46), 2001, Condition Assessment Scheme, IMO Resolution MEPC.111(50), 2003, Amendments to regulation 13G, addition of new regulation 13H involves a huge amount of measurement data to be collected, processed, analysed and maintained. Information to be recorded consists of thickness measurements and visual assessment of coating and cracks. The amount of data and increasing requirements with respect to condition assessment demand efficient computer support. Currently, due to the lack of standardization for this kind of data, the thickness measurements are recorded manually on ship drawings or tables. In this form, handling of the measurements is tedious and error-prone and assessment is difficult. Data reporting and analysis takes a long time, leading to some repairs being performed only at the next docking of the ship or making an additional docking necessary. The recently started ED funded project CAS addresses this topic and develops-as a first step-a data model for Hull Condition Monitoring and Assessment (HCMA) based on XML-technology. The model includes simple geometry representation to facilitate a graphically supported data collection as well as an easy visualisation of the measurement results. In order to ensure compatibility with the current way of working, the content of the data model is strictly confined to the requirements of the measurement process. Appropriate data interfaces to classification software will enable rapid assessment by the classification societies, thus improving the process in terms of time and cost savings. In particular, decision-making can be done while the ship is still in the dock for maintenance.