• 산업기술연구
• /
• 제20권A호
• /
• pp.45-50
• /
• 2000

A submerged cultivation of Ganoderma lucidum was carried out in an air-lift fermenter system, and the effects of different pH processes on extracellular branched ${\beta}$-1,3-glucan(EPS) production and mycelial growth(MDW) were investigated. The controlled pH process improved the production of branched ${\beta}$-1,3-glucan and biomass in comparison to the uncontrolled pH process. However, the maximum production of branched ${\beta}$-1,3-glucan were obtained by the bi-staged pH process. From these results, we confirmed that the bi-staged pH process was the most effective for improving the production of branched ${\beta}$-1,3-glucan from submerged culture of G. lucidum.

• 약학회지
• /
• 제37권3호
• /
• pp.216-227
• /
• 1993

Complex formations of practically insoluble antelmintic drugs such as mebendazole (MBZ), albendazole (ABZ) and flubendazole (FBZ) with dimethyl-$\beta$-cyclodextrin (DM-$\beta$-CyD) and 2-hydroxypropyl-$\beta$-cyclodextrin (HP-$\beta$-CyD) together with $\alpha$-, $\beta$- and $\gamma$-cyclodextrins(CyDs) in duffered solutions were investigated by solubility method. $A_{L}$ type phase solubility diagrams were obtained in all cases except for the complexation (B$_{s}$, type) of FBZ with $\gamma$-CyD. The highest stability constants were obtained with DM-$\beta$-CyD, followed by $\alpha$-CyD > $\beta$-CyD > HP-$\beta$-CyD > $\gamma$-CyD for ABZ, and HP-$\beta$-CyD > $\gamma$-CyD > $\beta$-CyD > $\alpha$-CyD for FBZ at pH 1.2. On the other hand, solid dispersion systems of ABZ and FBZ with $\beta$- and DM-$\beta$-CyDs were prepared by solvent evaporation method and evaluated by dissolution, differential thermal analysis and powder x-ray diffractometry. The dissolution rates of ABZ- and FBZ-DM-$\beta$-CyD solid dispersions were much faster than those of drugs alone, corresponding physical mixtures and tablets on market both at pH 1.2 and 6.8. Although dissolution rates of all samples at pH 6.8 were by far lower than those obtained at pH 1.2, as explained by pH-solubility profiles for ABZ and FBZ, the dissolution rates at pH 6.8 of ABZ from $\beta$- and DM-$\beta$-CyD solid dispersions exceeded the respective equilibrium solubility (23.9 $\mu\textrm{g}$/ml). Fast dissolution of ABZ from solid dispersions with CyDs was attributed to the reduction of drug crystallinity and particle size which was supported by DTA and powder x-ray diffractometry. Consequently these results suggest that solid dispersion systems with CyDs may provide useful means to markedly enhance the solubility and dissolution of benzimidazole antelmintic drugs.

• Animal cells and systems
• /
• 제9권2호
• /
• pp.101-105
• /
• 2005

[ ${\beta}ig-h3$ ] is a secretory protein that is induced by $TGF-{\beta}$ and implicated in various disease conditions including fibrosis. We have previously reported that ${\beta}ig-h3$ expression is implicated in astrocyte response to brain injury. In this study, we further investigated potential roles of ${\beta}ig-h3$ protein in the injured central nervous system (CNS). We specifically assessed whether the treatment of microglial cells with ${\beta}ig-h3$ can regulate microglial activity. Microglial cells are the prime effector cells in CNS immune and inflammatory responses. When activated, they produce a number of inflammatory mediators, which can promote neuronal injury. We prepared conditioned medium from the stable CHO cell line transfected with human ${\beta}ig-h3$ cDNA. We then examined the effects of the conditioned medium on the LPS- or $IFN-{\gamma}-mediated$ induction of proinflammatory molecules in microglial cells. Preincubation with the conditioned medium significantly attenuated LPS-mediated upregulation of $TNF-{\alpha},\;IL-1{\beta}$, iNOS and COX-2 mRNA expression in BV2 murine microglial cells. It also reduced $IFN-{\gamma}-mediated$ upregulation of $TNF-{\alpha}$ and COX-2 mRNA expression but not iNOS mRNA expression. Assays of nitric oxide release correlated with the mRNA data, which showed selective inhibition of LPS-mediated nitric oxide production. Although the regulatory mechanisms need to be further investigated, these results suggest that astrocyte-derived ${\beta}ig-h3$ may contribute to protection of the CNS from immune-mediated damage via controlling microglial inflammatory responses.

• Journal of Pharmaceutical Investigation
• /
• 제39권1호
• /
• pp.51-54
• /
• 2009

${\beta}ig$-h3, is a TGF-${\beta}$-induced gene product, extracellular matrix protein with 68 kDa MW(683 amino acids) and has been known for its possible roles in cell adhesion, spreading, migration and proliferation. To minimize a proteolytic degradation of ${\beta}ig$-h3, ${\beta}ig$-h3 incorporated chitosan sponge was prepared and its effects on fibroblast adhesion and migration were investigated. And its wound healing efficacy was evaluated in deep 2nd degree burn rabbit ear wound model. ${\beta}ig$-h3 enhanced fibroblast adhesion and proliferation. In histological observation, a significant over-proliferation of epidermal regeneration was observed in ${\beta}ig$-h3/chitosan dressing applied wound while epidermal regeneration was not proceeded yet in chitosan only treated wound. ${\beta}ig$-h3/sponge dressing could enhance epidermal regeneration.

• 한국미생물·생명공학회지
• /
• 제37권2호
• /
• pp.99-104
• /
• 2009

Streptomyces coelicolor A3(2)의 $\beta$-glucosidase 유전자를 분리하여 대장균에서 발현하여 특성을 조사하였다. 최적 활성을 나타내는 온도는 pH 5에서는 $20^{\circ}C$, pH 6에서는 $60^{\circ}C$에서 높은 활성을 나타냈다. pH에 따른 활성은 pH 3 이하와 pH 9 이상의 범위에서는 낮은 활성을 나타냈으며 pH 7에서 가장 높은 활성을 나타냈다. $\alpha$-pNPG($\rho$-nitrophenyl-$\alpha$-D-glucopyranoside), $\beta$-pNPG ($\rho$-nitrophenyl-$\beta$-D-glucopyranoside), $\beta$-pNPF($\\rho$-nitrophenyl-$\beta$-D-fucopyranoside)는 pH 3-10까지 비슷한 활성을 나타냈으며, $\alpha$-pNPG가 pH 7에서 다소 높은 활성을 보였다. $\beta$-pNPGA는 pH 5-9까지 높은 활성을 나타냈으며, 특히 pH 9에서 3배 이상의 높은 활성을 나타냈다. 기질 $\alpha$-pNPG, $\beta$-pNPG, $\beta$-pNPF의 온도에 따른 활성변화는 $\beta$-pNPF의 활성이 $60^{\circ}C$에서 증가하였고, $\beta$-pNPGA는 $30-50^{\circ}C$까지 활성이 증가하여 $50^{\circ}C$에서 최대활성을 나타내었다. 당화 flavonoid를 이용한 기질특이성의 상대활성은 daidzin, glycitin, genistin, 순으로 나타났으며 esculin과 apigenin-7-glucose는 기질로 사용하지 않았다. $\beta$-Glucosidase 활성은 EDTA, DTT에 의해 억제되었으며, $MnSO_4$, $CaCl_2$, KCl, $MgSO_4$에 의해 증가하였고, 특히 Mn이온에 의해 증가하였다. $CuSO_4$, NaCl에 의해 효소활성이 저해되었으며, 특히 $ZnSO_4$의 경우 효소활성이 강하게 억제되었다.

• 대한수학회보
• /
• 제52권1호
• /
• pp.105-123
• /
• 2015

Let $f_{\beta}=h_{\beta}+\bar{g}_{\beta}$ and $F_a=H_a+\bar{G}_a$ be harmonic mappings obtained by shearing of analytic mappings $h_{\beta}+g_{\beta}=1/(2isin{\beta})log\((1+ze^{i{\beta}})/(1+ze^{-i{\beta}})\)$, 0 < ${\beta}$ < ${\pi}$ and $H_a+G_a=z/(1-z)$, respectively. Kumar et al. [7] conjectured that if ${\omega}(z)=e^{i{\theta}}z^n({\theta}{\in}\mathbb{R},n{\in}\mathbb{N})$ and ${\omega}_a(z)=(a-z)/(1-az)$, $a{\in}(-1,1)$ are dilatations of $f_{\beta}$ and $F_a$, respectively, then $F_a\tilde{\ast}f_{\beta}{\in}S^0_H$ and is convex in the direction of the real axis, provided $a{\in}[(n-2)/(n+2),1)$. They claimed to have verified the result for n = 1, 2, 3 and 4 only. In the present paper, we settle the above conjecture, in the affirmative, for ${\beta}={\pi}/2$ and for all $n{\in}\mathbb{N}$.

• 한국식품영양과학회지
• /
• 제24권2호
• /
• pp.202-207
• /
• 1995

The effects of lipoxygenase, linoleic acid, tocopherol and pH on the co-oxidation of $\beta$-carotene in the aqueous system were studied. It showed that the co-oxidation of $\beta$-carotene was noticeable at both pH 7.4 and 9.0. As the concentraitons of linoleic acid and $\beta$-carotene increased, the rate of oxidation of $\beta$-carotene tended to be increased. However, $\alpha$- and $\delta$-tocopherol retarded the co-oxidation of $\beta$-carotene. As the concentrations of tocopherols increased, $\beta$-carotene was more stabilized, generally. But low concentration of $\alpha$-tocopherol(10-4M) acted more effective antioxidant than high concentration of it(10-3M) at pH 7.4. The antioxidant effect of tocopherol greatly depended on pH ; it was outstanding at pH 7.4.

• 미생물학회지
• /
• 제23권2호
• /
• pp.77-83
• /
• 1985

Sophorose에 의한 섬유소분해효소의 유도현상에 있어, Nisizawa등고 Strernbergdh Mandels가 연구보고한 상호 다른 결과들을 재규명하고, sophorose에 의한 섬유소분해효소 합성에 미치는 몇가지 요인들을 조사하고자 본 연구를 행하였다. Sophorose는 Trichoderma reesei QM914에서 CMCase와 ${\beta}-glucosidase$의 합성을 동시에 유도하며, CMCase는 pH 3.0~4.0의 완충용액을 갖는 유도배지에서, ${\beta}-glucosidase$는 pH 5.0~6.0의 K-citrate 완충용액을 갖는 유도배지에서 그 합성이 최대로 유도되었다. 또한, 세포내 ${\beta}-glucosidase$는 pH 6.5의 기질용액에 대하여, 세포의 ${\beta}-glucosidase$는 pH 5.0의 기질용액에 대하여, 각각 최대 활성도를 나타내었다. Methyl ${\beta} D glucosidase$ 는 ${\beta}-glucosidase$의 진정한 유도물질이 아닌 것으로 밝혀졌다. 포도당은 sophorose 에 의한 섬유소분해효소의 유도과정을 억제하며, 이 억제효과는 cAMP의 첨가에 의해서 영향을 받지 아니하였다.

• 한국동물번식학회:학술대회논문집
• /
• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
• /
• pp.69-69
• /
• 2003

Although effect of TGF$\beta$$_1$ on preimplantation embryo development was reported at mice, little information relevant to this subject is known in bovine. The objectives of this study were to investigate TGF$\beta$$_1$, and TGF$\beta$$_1$ receptors type I and II expression, known as important factors in the embryo development, at unfertilized oocytes and fertilized embryos that will be used as basic data to be compared to NT embryos. We postulated that TGF$\beta$$_1$ may have a beneficial effect on the preimplantation embryo and show different expression patterns as embryo stages change. We have used immunocytochemistry to investigate the presence in unfertilized oocytes and preimplantation embryos of TGF$\beta$$_1$ and the essential components of the TGF$\beta$$_1$ signalling pathway, TGF$\beta$$_1$ receptors type I and II. We found that both receptors, as well as TGF$\beta$$_1$, were present in the unfertilized oocytes. This indicates that TGF$\beta$$_1$, is a maternally expressed protein. At the morulae and blastocyst stages the TGF$\beta$$_1$ receptor type II was not present, but the TGF$\beta$$_1$ receptor type I was present at both stages and we can confirm the TGF$\beta$$_1$ expression of high level at 8-cell stage. These findings support our hypothesis that the TGF$\beta$$_1$, and TGF$\beta$$_1$ receptors may interact with the oocyte and preimplantation embryo, and that TGF$\beta$$_1$ signalling may be important for the development of the oocyte and the preimplahtation embryo.

• 대한수학회보
• /
• 제34권4호
• /
• pp.627-636
• /
• 1997

Let $H(U)$ be the space of all analytic functions in the unit disk $U$ and let $K \subset H(U)$. For the operator $A_{\beta,\gamma} : K \longrightarrow H(U)$ defined by $$ A_{\beta,\gamma}(f)(z) = [\frac{z^\gamma}{\beta + \gamma} \int_{0}^{z} f^\beta (t)t^{\gamma-1} dt]^{1/\beta} $$ and $\beta,\gamma \in C$, we determined conditions on g(z), $\beta and \gamma$ such that $$ z[\frac{z}{f(z)]^\beta \prec z[\frac{z}{g(z)]^\beta implies z[\frac{z}{A_{\beta,\gamma}(f)(z)]^\beta \prec z[\frac{z}{A_{\beta,\gamma}(g)(z)]^\beta $$ and we presented some particular cases of our main result.