• 한국미생물·생명공학회지
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• 제36권2호
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• pp.128-134
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• 2008

Erro-prone PCR에 의해서 열안정성이 향상된 Streptomyces coelicolor A(3) 유래의 L-threonine aldolase를 Corynebacterium glutamicum R에서 과잉발현시키기 위하여 Corynebacterium용 vector plasmid인 pCRB1의 SD배열과 개시코든사이의 1염기를 제거한 고발현용 vector plasmid인 pCG-H44(2)를 구축하였다. pCG-H (2)에 의해서 형질전환된 C. glutamicum R 균주(CGH44-2)에서 L-TA를 발현시킨 결과, 기존의 Corynebacterium용 vector plasmid인 pCRB1(CGH44-1)보다 L-TA의 발현량이 높았다. L-threo-DOPS의 합성을 위한 최적조건은 $30^{\circ}C$, 0.1 M cirtric acid buffer(pH 7.0)이었으며, 0.1% TritonX-100를 첨가하였을 경우 보다 높은 활성을 보였다. 최적조건하에서 CGH44-2를 whole cell biocatalyst로 이용한 반복회분식반응에서 재조합대장균을 숙주로 이용한 경우보다 재조합Corynebacterium을 이용하였을 경우, 목적하는 L-threo-DOPS의 합성이 안정적으로 이루어졌다.

• Bulletin of the Korean Chemical Society
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• 제31권6호
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• pp.1573-1576
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• 2010

Bacillus subtilis PerR is a metal-dependent peroxide-sensing transcription factor which uses metal-catalyzed histidine oxidation for peroxide-sensing. PerR contains two metal binding sites, one for structural $Zn^{2+}$ and the other for the regulatory/peroxide-sensing metal. Here we investigated the effect of mutations at both the structural and regulatory metal binding sites on the oxidation of either H37 or H91, two of the peroxide-sensing ligands. All four serine substitution mutants at the structural $Zn^{2+}$ site (C96S, C99S, C136S and C139S) exhibited no detectable oxidation at histidine residues. Two of the alanine substitution mutants at regulatory metal site (H37A and D85A) exhibited selective oxidation preferentially at the H91-containing tryptic peptide, whereas no oxidation was detected in the other mutants (H91A, H93A and D104A). Our results suggest that the cysteine residues coordinating structural $Zn^{2+}$ are essential for peroxide sensing by PerR, and that the C-terminal regulatory metal binding site composed of H91, H93 and D104 can bind $Fe^{2+}$, providing a possible explanation for the peroxide sensing mechanisms by PerR.

• 한국결정학회지
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• 제12권4호
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• pp.212-215
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• 2001

NaOAC·3H₂O 존재 하에서, manganese nitrate(Mn(NO₃)₂·H₂O )와 imidzole-4,5-dicarboxylic acid(IDCH₂)가 수열반응하여, 실험식 [Mn(IDC)(H₂O)]을 가지는 2차원 배위고분자 합성되었다. 화합물 1의 구조가 분광학적 방법(IR) 및 X-ray 회절법으로 규명되었다. 화합물의 1의 결정학적 자료: 사방정계 공간군 Pbca, a=7.257(5) Å b=13.687(5)Å, c=14.332(6)Å Z=8, R(wR₂)=0.0498(0.0999).

• 한국결정학회지
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• 제6권2호
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• pp.93-97
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• 1995

Re(≡NC6H5)(PPh3)2CI3 화합물이 1,2-bis(diphenylphosphino)ethane (DPPE)와 반응하여 fac-Re(≡NC6H5)(DPPE)CI3이 되었다. 이 생성물의 구조를 1H-NMR, 원소분석, 그리고 X-ray 회절법으로 규명하였다. 이 생성물은 단사정계로 (Pc, a=11.083(3)Å, b=10.930(1)Å, c=14.081(2)Å, β=108.37(2)°, Z=2) 결정화되었다. 최소자승법으로 구조를 정밀화한 결과 신뢰도는 R(wR2)=0.0254(0.0607)이였다.

• 센서학회지
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• 제32권4호
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• pp.207-212
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• 2023

H₂S is a toxic and harmful gas, even at concentrations as low as hundreds of parts per million; thus, developing an H₂S sensor with excellent performance in terms of high response, good selectivity, and fast response time is important. In this study, an H₂S sensor with a high response and fast response time, consisting of a sensing material (SnO₂), an electrode, a temperature sensor, and a micro-heater, was developed using micro-electro-mechanical system technology. The developed H₂S sensor with a micro-heater (circular type) has excellent H₂S detection performance at low H₂S concentrations (0-10 ppm), with quick response time (<16 s) and recovery time (<65 s). Therefore, we expect that the developed H₂S sensor will be considered a promising candidate for protecting workers and the general population and for responding to tightened regulations.

• Restorative Dentistry and Endodontics
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• 제27권6호
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• pp.632-643
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• 2002

The purpose of this study was two-fold. First was to evaluate whether the molecular sieving model was appropriate for ionic dissociation experiment. Second was to compare the dissociation of calcium and hydroxyl ions from five types of calcium hydroxide pastes (Pure calcium hydroxide paste, DT temporary $dressing^{\circledR},{\;}Metapaste^{\circledR},{\;}Chidopex^{\circledR},{\;}Metapex^{\circledR}$) in three vehicles (aqueous, viscous and oily) and the antibacterial effect. Each calcium hydroxide pastes was placed into 0.65ml tube with cap and then 15% polyacrylamide gel was placed onto calcium hydroxide pastes. After the gel was hardened, the tubes were filled with tridistilled water (pH 7.14) and closed with cap. The tubes were stored in $37^{\circ}C$ 100% incubator The pH reading and the concentration of calcium ions were taken at 1, 4, 7. 10, and 14 days. The brain heart infusion agar plates with S. mutans and A. actinomycetemcomitans were used far antibacterial activity test. Middle of agar plate was filled with the calcium hydroxide pastes. The plates were incubated at $37^{\circ}C$ and observations were made to detect the zones of inhibition. These data were evaluated statistically by use of the analysis of variance and duncan test. The results were as follows. 1. In fresh mixing state, the pH of five types of calcium hydroxide pastes were measured between 12.5 and 12.8. 2. The pH was increased in all five types of calcium hydroxide pastes compared with control group. In 14 days, Pure calcium hydroxide paste (11.45) and DT temporary $dressing^{\circledR}$ (11.33) showed highest pH, followed by $Metapaste^{\circledR}$ (9.49), $Chidopex^{\circledR}$ (8.37) and $Metapex^{\circledR}$ (7.59) 3. Calcium was higher in all five types of calcium hydroxide pastes compared with control group. In 14 days, Pure calcium hydroxide paste (137.29 mg%) and DT temporary $dressing^{\circledR}$ (124.6 mg%) showed highest value, followed by $Metapaste^{\circledR}$ (116.74 mg%), $Chidopex^{\circledR}$ (111.84 mg%) and $Metapex^{\circledR}$ (60.22 mg%). 4. The zones of bacterial inhibition were seen around all five types of calcium hydroxide pastes. $Chidopex^{\circledR}{\;}and{\;}Metapex^{\circledR}$ groups which include iodoform were observed significantly larger zone of inhibition in A. actinomycetemcomitans compared with the other calcium hydroxide groups (p<0.05) However, $Metapex^{\circledR}$ showed the least antibacterial effect on S. mutans compared with other groups (p<0.05). The molecular sieving model was found to be acceptable in dissociation experiment of hydroxyl and calcium ions when compared with the previous tooth model study. But this model was not appropriate for the antibacterial test.

• 대한수학회보
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• 제61권1호
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• pp.273-280
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• 2024

Let I be an ideal of a commutative Noetherian semi-local ring R and M be an R-module. It is shown that if dim M ≤ 2 and Supp_R M ⊆ V (I), then M is I-weakly cofinite if (and only if) the R-modules Hom_R(R/I, M) and Ext¹_R(R/I, M) are weakly Laskerian. As a consequence of this result, it is shown that the category of all I-weakly cofinite modules X with dim X ≤ 2, forms an Abelian subcategory of the category of all R-modules. Finally, it is shown that if dim R/I ≤ 2, then for each pair of finitely generated R-modules M and N and each pair of the integers i, j ≥ 0, the R-modules Tor^R_i(N, H^j_I(M)) and Extⁱ_R(N, H^j_I(M)) are I-weakly cofinite.

• Journal of Advanced Marine Engineering and Technology
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• 제29권1호
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• pp.49-59
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• 2005

The evaporation heat transfer experiments are conducted with the shell and plate heat exchanger (S&PHE) without oil in the refrigerant loop using R-410A. An experimental refrigerant loop has been established to measure the evaporation heat transfer coefficient h. of R-410A in a vertical S&PHE. Two vertical counter flow channels were formed in the S&PHE by three plates haying a corrugated trapezoid shape of a $45^{\circ}C$ chevron angle. UP flow of the boiling R-410A in one channel receives heat from the hot down flow of water in the other channel The effects of the refrigerant mass flux. average heat flux. refrigerant saturation temperature and vapor qualify are explored in detail. Similar to the case of a plate heat exchanger. even at a very low Reynolds number, the flow in the S&PHE remains turbulent. The Present data shows that the evaporation heat transfer coefficients of R-410A increased with the vapor qualify. The results indicate a rise in the refrigerant mass flux caused an increase in the h.. Raising the imposed wall heat flux is found to slightly improve h., while h, is found to be lower at a higher refrigerant saturation temperature. Based on the present data. empirical correlation of the evaporation heat transfer coefficient is proposed.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2006년도 춘계학술대회 논문집
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• pp.545-546
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• 2006

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• 대한전기학회:학술대회논문집
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• 대한전기학회 2004년도 춘계학술대회 논문집 전기설비전문위원
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• pp.88-90
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• 2004

설비의 가용성 및 신뢰성 증대와 보수 정비 비용 절감에 대한 관심이 증대되면서 전력시스템의 유지보수에 대한 관심이 증대되고 있다. 계획에 없는 유지보수와 예상치 못한 정전 등을 최소화시키면서 전체설비의 활용도와 신뢰도를 증가시키는 방법에 대한 요구가 커지고 있는 것이다. 기존의 계획기반의 유지보수에서 최근 도입되고 있는 상태 감시기반의 유지보수는 설비의 상태를 감시, 분석하여 유지 보수함으로써 진일보되었으나, 기술적, 비용적인 문제로 전면적인 도입이 힘든 상황이다. 따라서 각 설비의 특성 파악과 운용 환경에 따른 설비의 중요도 및 파급효과 분석을 통해 가장 효율적이고 경제적인 유지보수 기법을 결정하는 신뢰성 기반의 예방진단 기법인 RCM의 적용에 관한 분석사항 등을 살펴보았다.