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Enhancement Pattern of the Normal Facial Nerve on Three - Dimensional (3D) - Fluid Attenuated Inversion Recovery (FLAIR) Sequence at 3.0 T MR Units (3.0T 자기공명영상기기에서 시행한 3D-FLAIR 영상에서의 정상 안면신경의 조영증강 양상)

  • Hyun, Dong-Ho;Lim, Hyun-Kyung;Park, Jee-Won;Kim, Jong-Lim;Lee, Ha-Young;Park, Soon-Chan;Ahn, Joong-Ho;Baek, Jung-Hwan;Choi, Choong-Gon;Lee, Jeong-Hyun
    • Investigative Magnetic Resonance Imaging
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    • v.16 no.1
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    • pp.25-30
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    • 2012
  • Purpose : To compare the enhancement pattern of normal facial nerves on 3D-FLAIR and 3D-T1-FFE-F) sequences at 3.0 T MR units. Materials and Methods: We assessed 20 consecutive subjects without a history of facial nerve abnormalities who underwent temporal bone MRI with contrast enhancement between January 2008 and March 2009. Two neuroradiologists independently reviewed pre-/post-enhanced 3D-T1-FFE-FS and 3D-FLAIR images respectively with 2-week interval to assess the enhancement of normal facial nerves divided into five anatomical segments. The degree of enhancement in each segment was graded as none, mild or strong, and the results of 3D-FLAIR and 3D-T1-FFE-FS image sets were compared. Results: On 3D-FLAIR images, one of the two reviewers observed mild enhancement of the genu segment in two (10%) subjects. On 3D-T1-FFE-FS images, at least one segment of the facial nerve was enhanced in 13 (65%) subjects. At least one reviewer found that 17 of the 100 segments showed enhancement on 3D-T1-FFE-FS images, with the mastoid segment being the most commonly enhanced. Interobserver agreement on 3D-T1-FFE-FS images was good for enhancement of the normal facial nerve (${\kappa}$= 0.589). Conclusion: In contrast to 3D-T1-FFE-FS, normal facial nerve segments rarely showed enhancement on 3D-FLAIR images.

Effects of Dietary Supplementation of Bacteriophage on Growth Performance, Nutrient Digestibility, Blood Profiles, Carcass Characteristics and Fecal Microflora in Broilers (육계 사료 내 Bacteriophage의 첨가가 생산성, 영양소 소화율, 혈액 특성, 도체 특성 및 분내 미생물 조성에 미치는 영향)

  • Kim, Seung Cheol;Kim, Jae Won;Kim, Jung Un;Kim, In Ho
    • Korean Journal of Poultry Science
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    • v.40 no.1
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    • pp.75-81
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    • 2013
  • This experiment was conducted to investigate the effects of bacteriophage SE supplementation on growth performance, nutrient digestibility, blood profiles, visceral organ weight, meat quality and excreta microflora in broilers. A total of 340 1-d-old ROSS 308 broilers (mixed gender) with an initial average body weight (BW) of $41.71{\pm}0.16$ g were randomly allotted to 4 treatments with 5 replicate pens per treatment and 17 broilers per pen for 31 days. Dietary treatments were: 1) CON, control diet, 2) SE05, CON+0.05% bacteriophage, SE 3) SE10, CON+0.10% bacteriophage SE, and 4) SE15, CON+0.15% bacteriophage SE. During d 15 to 31, broilers fed SE15 diet had a higher (P<0.05) body weight gain than broilers fed CON diet. Overall, body weight gain in SE10 and SE15 was greater (P<0.05) than that in CON. Apparent total tract nutrient digestibility and blood characteristics did not differ (P>0.05) among treatments. The water holding capacity was increased (P<0.05) in SE15 compared with CON. Other meat quality in terms of pH value, breast muscle color ($L^*$, $a^*$, $b^*$) and drip loss were unaffected by dietary supplementation with bacteriophage SE. The visceral weight of bursa of Fabricius was increased (P<0.05) in broilers fed the bacteriophage SE incorporated diets compared with those fed the CON diet. No difference (P>0.05) was observed in visceral weight of liver, spleen, breast muscle, abdominal fat, gizzard and excreta concentrations of Lactobacillus, Clostridium perfringens, Escherichia coli, and Salmonella. In conclusion, dietary supplementation with 0.10 and 0.15% bacteriophage SE could improve the growth performance, breast muscle water holding capacity and bursa of Fabricius visceral weight in broilers.

Recovery of Lipids from Chlorella sp. KR-1 via Pyrolysis and Characteristics of the Pyrolysis Oil (Chlorella sp. KR-1 열분해에 의한 지질 회수 및 열분해 오일 특성 분석)

  • Lee, Ho Se;Jeon, Sang Goo;Oh, You-Kwan;Kim, Kwang Ho;Chung, Soo Hyun;Na, Jeong-Geol;Yeo, Sang-Do
    • Korean Chemical Engineering Research
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    • v.50 no.4
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    • pp.672-677
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    • 2012
  • Lipids in microalgal biomass were recovered by using pyrolysis method. The pyrolysis experiments of two Chlorella sp. KR-1 samples, which have triglyceride contents of 10.8% and 36.5%, respectively were carried out at $600^{\circ}C$ to investigate the effects of lipid contents in the cells on the reaction characteristics. The conversion and liquid yield of the lipid-rich sample were higher than those of the lipid-lean sample since its carbon to hydrogen ratio was low. There were low molecular weight organic acids, ketones, aldehydes and alcohols in the liquid products from both KR-1 samples, but the pyrolysis oil of the lipid-rich sample was abundant in free fatty acids, particularly palmitic acid, oleic acid and stearic acid while the content of nitrogen containing organic compounds was low. The microalgal pyrolysis oil had two layers composed of the light hydrophobic fraction and the heavy hydrophilic fraction. The light fraction might be originated from triglycerides and the heavy fraction might be from carbohydrates and proteins. In the light fraction of the liquid products, there were considerable linear alkanes such as pentadecane and heptadecane as well as free fatty acids, implying that deoxygenation reaction including decarboxylation was occurred during the pyrolysis. The yield of the liquid products from the pyrolysis of the KR-1 sample having triglyceride content of 36.5% was 56.9% and the light fraction in the liquid products was 68.2%. Also more than 80% of the light fraction was free fatty acids and pure hydrocarbons, thus showing that most triglycerides could be extracted in the form of suitable raw materials for biofuels.

Prediction of future hydrologic variables of Asia using RCP scenario and global hydrology model (RCP 시나리오 및 전지구 수문 모형을 활용한 아시아 미래 수문인자 예측)

  • Kim, Dawun;Kim, Daeun;Kang, Seok-koo;Choi, Minha
    • Journal of Korea Water Resources Association
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    • v.49 no.6
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    • pp.551-563
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    • 2016
  • According to the 4th and 5th assessment of the Intergovernmental Panel on Climate Change (IPCC), global climate has been rapidly changing because of the human activities since Industrial Revolution. The perceived changes were appeared strongly in temperature and concentration of carbon dioxide ($CO_2$). Global average temperature has increased about $0.74^{\circ}C$ over last 100 years (IPCC, 2007) and concentration of $CO_2$ is unprecedented in at least the last 800,000 years (IPCC, 2014). These phenomena influence precipitation, evapotranspiration and soil moisture which have an important role in hydrology, and that is the reason why there is a necessity to study climate change. In this study, Asia region was selected to simulate primary energy index from 1951 to 2100. To predict future climate change effect, Common Land Model (CLM) which is used for various fields across the world was employed. The forcing data was Representative Concentration Pathway (RCP) data which is the newest greenhouse gas emission scenario published in IPCC 5th assessment. Validation of net radiation ($R_n$), sensible heat flux (H), latent heat flux (LE) for historical period was performed with 5 flux tower site-data in the region of AsiaFlux and the monthly trends of simulation results were almost equaled to observation data. The simulation results for 2006-2100 showed almost stable net radiation, slightly decreasing sensible heat flux and quite increasing latent heat flux. Especially the uptrend for RCP 8.5 has been about doubled compared to RCP 4.5 and since late 2060s, variations of net radiation and sensible heat flux would be significantly risen becoming an extreme climate condition. In a follow-up study, a simulation for energy index and hydrological index under the detailed condition will be conducted with various scenario established from this study.

Effect of SeaR gene on virginiamycins production in Streptomyces virginiae (희소방선균 SeaR 유전자가 Streptomyces virginiae의 virginiamycins 생산에 미치는 영향)

  • Ryu, Jae-Ki;Kim, Hyun-Kyung;Kim, Byung-Won;Kim, Dong-Chan;Lee, Hyeong-Seon
    • Korean Journal of Microbiology
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    • v.51 no.3
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    • pp.256-262
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    • 2015
  • In order to study the effect of the receptor protein (SeaR), which is isolated from Saccharopolyspora erythraea, we introduced the SeaR gene to Streptomyces virginiae as host strains. An effective transformation procedure for S. virginiae was established based on transconjugation by Escherichia coli ET12567/pUZ8002 with a ${\varphi}C31$-derived integration vector, pSET152, which contained int, oriT, attP, and $ermEp^{\ast}$ (erythromycin promotor). Therefore, the pEV615 was introduced into S. virginiae by conjugation and integrated at the attB locus in the chromosome of the recipients by the ${\varphi}C31$ integrase (int) function. Transformants of S. virginiae containing the SeaR gene were confirmed by PCR and transcriptional expression of the SeaR gene in the transformants was analyzed by RT-PCR, respectively. And, we examined the production time of virginiamycins in the culture media of both the transformants and the wild type. The production time of virginiamycins in the wild type and transformants was the same. When 100 ng/ml of synthetic $VB-C_6$ was added to the state of 6 or 8 hour cultivation of wild type and transformants, respectively, the virginiamycins production was induced, meaning that the virginiamycins production in the wild type was detected 2 h early than transformants. From these results, SeaR expression was also affected to virginiamycins production in transformants derived from S. virginiae. In this study, we showed that the SeaR protein worked as a repressor in transformants.

Nose Changes after Maxillary Advancement Surgery in Skeletal Class III Malocclusion (골격성 III급 부정교합자에서 상악골 전방 이동술 후 코의 변화에 관한 연구)

  • Kang, Eun-Hee;Park, Soo-Byung;Kim, Jong-Ryoul
    • The korean journal of orthodontics
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    • v.30 no.5 s.82
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    • pp.657-668
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    • 2000
  • The purpose of this study was to evaluate the amount and interrelationship of the soft tissue of nose and maxillary changes and to identify the nasal morphologic features that indicate susceptibility to nasal deflection in such a manner that they would be useful in presurgical prediction of nasal changes after maxillary advancement surgery in skeletal Class III malocclusion. The sample consisted of 25 adult patients (13 males and 12 females) who had severe anteroposterior skeletal discrepancy. The patients had received presurgical orthodontic treatment. They underwent a Le Fort I advancement osteotomy, rigid internal fixation, alar cinch suture and V-Y advancement lip closure. The presurgical and postsurgical lateral cephalograms and lateral and frontal facial photographs were evaluated. The computerized statistical analysis was carried out. Soft tissue of nose change to h point change ratios were calculated by regression equations. The results were as follows 1. The correlation of maxillary hard tissue horizontal changes and nasal soft tissue vortical changes were high and the ${\beta}_0$ for soft tissue to ADV were 0.228 at ANt, 0.257 at SNt. 2. The correlation of maxillary hard tissue and nasal soft tissue horizontal changes were high and the ${\beta}_0$ for soft tissue to ADV were 0.484 at ANt, 0.431 at SNt, 0.806 at Sn. 3. The correlation of maxillary hard tissue horizontal changes and width changes of ala of nose were high and the ${\beta}_0$ lot alar base width ratio to ADV were 0.002. 4. The DRI, Prominence of nose, Pre-Op CA is not a quantitative measure that can be used clinically to improve the predictability of vertical and horizontal nasal tip deflection. In this study, increases in nasal tip projection and anterosuperior rotation occur when there is an anterior vector of maxillary movement. These nasal changes were Quantitatively correlated to magnitude of maxillary(A point) movement.

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A Land Resources Survey of the Mula Area, S. E. Spain (동남(東南)스페인 Mula지역(地域)에서의 Land Resources Survey)

  • Yun, Suckew
    • Economic and Environmental Geology
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    • v.6 no.1
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    • pp.29-64
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    • 1973
  • A land resources survey in the semi-arid area, Mula in S. E. Spain, of $400km^2$ is compiled. The basic aim of the project is to investigate the intrinsic qualities of the land resources of the area by means of applying an integrated method of natural resources survey mainly concerning with analysis and synthesis of land complexes, each of them represents an area or a group of areas with similar patterns of landforms, soils and vegetation, based on a geomorphological approach. The area is characterized by a linear arrangement of relief pattern with an asymmetric homoclinal repetition of slope attitudes elongating WSW-ENE, dipping steeply on the NW sides and gently on the SE sides, which have been resulted from the post-Alpine folding of the Triassic to Cretaceous limestone, the Eocene limestone, the Oligocene sandstone and the lower Miocene limestone and marl, and the post-lower Miocene faulting, tilting and subsequent differential erosion of the Miocene sedimentary formations. An integrated body of information in geology, landforms, soils and vegetation, which are significantry interrelated as an environmental complex, has been obtained. Using this data, 26 land complexes developing on the various situations of landforms, such as folded mountain ranges, tilted tablelands, bevelled cuestas, degraded hill-lands associating with enormous foots lopes, undulating terrains and terraced or flat plains, have been differentiated, mapped and described. The soils of the area are mostly light colored calcic lithosols which have been derived dominantly from the marly parent materials and developed into remarkable slope catenas in some places depending on the relief conditions. The land uses of the area are mainly characterized by the perennially irrigated cultivation of citrus orchards along the terraced alluvial deposits fringing the Segura and Mula River, and the dry-land cereal cultivation on gentler slopes. Pioneer dry-land cultivations within the shrubs on steeper slopes are restricted to the unchannelled tributary drainage floors. The availability of water is a fundamental controlling factor for existence of native and cultivated vegetation as a whole, and a number of active processes including sheet wash and gully erosion, especially on the scarp slopes, are the other important factors to be considered in conservation and management of the land in the area.

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Physiological Activities of Ginkgo biloba Sarcotesta Extract with Heat Treatment (열처리에 따른 은행 외종피 추출물의 생리활성)

  • Kim, Sung Tae;Lee, Ji Hyun;Lee, Sang Hoon;Jang, Gwi Yeong;Li, Meishan;Kim, Min Young;Yoon, Nara;Lee, Junsoo;Jeong, Heon Sang
    • The Korean Journal of Food And Nutrition
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    • v.28 no.3
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    • pp.369-375
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    • 2015
  • This study was performed to investigate the physiological activities of Ginkgo biloba sarcotesta extracts before and after heat treatment. G. biloba sarcotesta was heated at $130^{\circ}C$ for 2 h and extracted with water, 70% ethanol and 80% methanol. ABTS and DPPH radical scavenging activities increased after heating in the water (14.95 mg AAE/g and 7.36 mg TE/g) and ethanol extracts (12.20 mg AAE/g and 6.23 mg TE/g). ${\alpha}$-Glucosidase inhibitory activity decreased after heating in all but the water extract. Angiotensin converting enzyme I inhibitory activities decreased after heating in all extracts. Nitric oxide production inhibitory activity increased from 12.40~44.55% of the raw sample to 40.76~72.39% of the heated sample at a concentration of $200{\mu}g/mL$. Lipid accumulation inhibitory activities were similar before and after heat treatment. The highest antiproliferative effects on MCF-7 human breast cancer cell lines were observed in 80% methanol extract in the heated sample. Cell viability at concentrations of 25, 50, 100, and $200{\mu}g/mL$ measured 34.88, 17.58, 8.44 and 10.48%, respectively. From the results, the antioxidant and antiproliferative activities of G. biloba sarcotesta extracts increased with heat treatment, and research on the identification of the structure for the active compounds are needed in further studies.

The Effects of Using Artificial Sweeteners and Coffee Grounds in Chocolate Filling on Quality Characteristics and Glycemic Index (쉘 초콜릿 filling에 사용한 대체 감미료와 커피박이 품질 특성 및 당지수에 미치는 영향)

  • Kang, Suna;Lee, Jin Sook;Jeong, Areum;Kim, Eunha;Park, Sunmin
    • Journal of Applied Biological Chemistry
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    • v.57 no.4
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    • pp.307-312
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    • 2014
  • Chocolate is restricted for obese and diabetic patients due to high in sugar contents. We investigated shell-chocolate fillings with low calorie content and low glycemic indexes using alternative sweeteners such as tagatose and xylose and adding coffee grounds. Chocolate filling was made with sugar, cacao and butter in the original recipe. Sugar was substituted with tagatose or xylose and 3% coffee ground was added in each chocolate filling and substituted for 3% of the cocoa. Water retention and spreadability of the chocolate filling was significantly higher in the tagatose+coffee ground group in comparison to the sugar. In the chromaticity test, chocolate filling made with tagatose was darker in comparison to that made with sugar: brightness (L) was lower and redness (a) and yellowness (b) were higher than the sugar filling. Sensory evaluation revealed that chocolate filling made with tagatose had high score in 4 categories such as taste, smell, texture of foreign substance, overall acceptance. After consuming 60 g of the chocolate with different fillings, blood glucose levels of subjects at 1 and 2 h were significantly lower in the tagatose and tagatose+coffee ground groups than the other groups. In conclusion, using tagatose as the sweetener in chocolate filling has a beneficial effect on lowering blood glucose levels and increasing water retention, spreadability and sensory scores. Coffee ground did not have additive effect on quality of chocolate filling and blood glucose levels. These results indicate that shell chocolate made with tagatose can be used as a snack for diabetic patients.

The Study on the Relationship between Changes of Rumen Microflora and Bloat in Jersey Cow (저지종 젖소의 반추위 내 미생물 균총 변화와 고창증 발병간의 상관관계 연구)

  • Kim, Sang Bum;Oh, Jong Seok;Jeong, Ha Yeon;Jung, Young Hun;Park, Beom Young;Ha, Seung Min;Im, Seok Ki;Lee, Sung Sill;Park, Ji Hoo;Park, Seong Min;Kim, Eun Tae
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.38 no.2
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    • pp.106-111
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    • 2018
  • This study was conducted to investigate the relationship between changes of rumen microflora and bloat in Jersey cow. Jersey cows (control age: 42 months, control weight: 558kg; treatment age: 29 months, treatment weight 507kg) were fed on the basis of dairy feeding management at dairy science division in National Institute of Animal Science. The change of microbial population in rumen was analyzed by using next generation sequencing (NGS) technologies due to metabolic disease. The diversity of Ruminococcus bromii, Bifidobacterium pseudolongum, Bifidobacterium merycicum and Butyrivibrio fibrisolvens known as major starch fermenting bacteria was increased more than 36-fold in bloated Jersey, while cellulolytic bacteria community such as Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens was increased more than 12-fold in non-bloated Jersey. The proportion of bacteroidetes and firmicutes was 33.4% and 39.6% in non-bloated Jersey's rumen, while bacteroidetes and firmicutes were 24.9% and 55.1% in bloated Jersey's. In conclusion, the change of rumen microbial community, in particular the increase in starch fermenting bacteria, might have an effect to occur the bloat in Jersey cow.