• Title/Summary/Keyword: H$_{d}$

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The Transfection of Caldesmon DNA into Primary Cultured Rat Aortic Vascular Smooth Muscle

  • Choi, Woong;Ahn, Hee-Yul
    • The Korean Journal of Physiology and Pharmacology
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    • v.3 no.6
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    • pp.597-603
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    • 1999
  • Caldesmon (CaD), one of microfilament-associated proteins, plays a key role in microfilament assembly in mitosis. We have investigated the effects of overexpression of the high molecular weight isoform of CaD (h-CaD) on the physiology of vascular smooth muscle cells (VSMCs). Rat aortic VSMCs were stably transfected with plasmids carrying a full length human h-CaD cDNA under control of cytomegalovirus promoter. The majority of the overexpressed h-CaD appears to be localized predominantly on cytoskeleton structures as determined by detergent lysis. The overexpression of h-CaD, however, does not decrease the level of endogenous low molecular weight isoform of CaD. h-CaD overexpressing VSMCs (h-CaD/VSMCs) show a decreased growth rate than that of vector-only transfected cells when determined by $[^3H]thymidine$ uptake and cell counting after fetal bovine serum (FBS) stimulation. h-CaD/VSMCs were smaller than vector-transfected cells by 18% in cell diameter. These data suggest that overexpression of h-CaD can inhibit the poliferation and the cell volume of VSMCs stimulated by growth factors and that the gene therapy with h-CaD may be helpful to prevent the conditions associated with hypertrophy and/or hyperplasia of VSMCs after arterial injuries.

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The Developmental Characterization of the Sericin jam(Bombyx mori), a Mutant in the Synthesis and Secretion of Fibroin (세리신잠(Nd-s. $Nd^H$의 발육 특성)

  • 김수연;손해룡
    • Journal of Sericultural and Entomological Science
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    • v.45 no.1
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    • pp.6-9
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    • 2003
  • This experiment was done to know developmental characteristics of Sericin jam. Incubation periods were 10 day 2 hr, and 10 day 1 hr. 11 day 1 hr for Nd-s jam, N $d^{H}$ jam, and Baegok jam, respectively. Hatching rates were 83.9, 83.3 and 96.0% for Nd-s jam, N $d^{H}$ jam, and Baegok jam. Larval periods were, 20 days 1 hr for Nd-s jam, 20 days 5 hrs for N $d^{H}$ jam, and 22 days 12 hrs for Baegok jam. Death rate of larvae was highest in N $d^{H}$ iam, followed by Nd-s jam and Baegok jam. Pupation rate was highest in Baegok Jam followed by Nd-s jam and that of N $d^{H}$ jam was the lowest among the three. Cocoon weight was 1.39, 1.08, and 2.01 g for Nd-s jam, N $d^{H}$ jam, and Baegok jam, respectively. Shell weight were 13, 3, and 48 cg for Nd-s jam, N $d^{H}$ jam, and Baegok jam. Cocoon shell ratios were 9.0% for Nd-s jam, 2.8% for N $d^{H}$ jam and 23.9% for Baegok jam. Cocoon sizes were 30.6${\times}$15.8 mm for Nd-s jam, 24.7${\times}$14.9 mm for N $d^{H}$ jam and 35.8 ${\times}$ 20.5 mm(1${\times}$w) for Baegok jam.w) for Baegok jam.

Dietary Effect of Hemicellulose from Soy Fiber on Blood Glucose and Cholesterol Content in Streptozotocin-Induced Diabetic Rats (콩 식이섬유로부터 추출한 헤미셀룰로즈의 식이가 Streptozotocin유도 당뇨 흰쥐의 혈당과 혈청 콜레스테롤 함량에 미치는 영향)

  • 이명예;김미경;신진기;김순동
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.7
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    • pp.1119-1125
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    • 2004
  • The effect of hemicellulose extracted from soy fiber on the level of blood glucose and serum cholesterol in the streptozotocin-induced diabetic rat were investigated. The experimental plots were divided to cellulose group (control, 0.5% hemicellulose group (H-l) and 1% hemicellulose group (H-2) group. The groups were fed for 6 weeks, then fed for 1 week more after streptozotocin injection. Food intakes, weight gain and food efficiency ratio of H-2 group were higher, while water intakes and liver weight were lower than those of control and H-l group. The content of blood glucose and urine glucose were 212.8 mg/dL, 0.97 mg/dL in the control group, 160.5 mg/dL, 0.53 mg/dL in the H-l group, 141.0 mg/dL, 0.35 mg/dL in the H-2 group, respectively. There was no significantly difference in the content of neutral lipid, while the content of total serum cholesterol was 101.6 mg/dL in the control group, 73.8-78.4 mg/dL in the hemicellulose groups. The content of serum HDL-cholesterol in the all experimental groups showed no significantly difference showing 39.8-44.7 mg/dL. HTR and atherogenic index were 0.44 and 1.27 in the control group, but 0.54 and 0.46-0.85 in the hemicellulose groups, respectively.

Arginase Activity in Response to pH Change in Canavalia Lineata Callus (해녀콩 캘러스에서 pH 변화에 따른 Arginase의 활성)

  • 이주용
    • Journal of Plant Biology
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    • v.36 no.4
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    • pp.407-413
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    • 1993
  • 해녀콩(Canavalia lineata L. DC) 잎에서 유도한 캘러스를 pH 8인 완충용액에 한 시간 동안 처리하면 pH 6에서 보다 arginase의 활성이 약 두 배로 증가했다. 캘러스에서 얻은 arginase의 조효소액을 Sephacryl S-200 컬럼에서 분획하면 분자량이 각각 380 kD과 179 kD으로 나타났는데 분자량이 큰 arginase의 분획은 pH 8 처리구에서 각각 상대적으로 많이 나타났다. 그리고 pH 6에 0.5 mM Mn2+를 첨가하였을 때도 380 kD arginase의 분획이 크게 나타났으며, pH 6 처리에서 얻은 179 kD arginase 분획에 pH 8 처리를 하면 380 kD 분획으로 쉽게 전이될 수 있었다. pH 6 처리 후 추출한 arginase는 Mn2+의 첨가로 활성이 크게 증가하여 pH 8 처리 후 추출한 arginase와 비슷한 활성을 보이나, pH 8 처리 후 추출한 arginase는 Mn2+의 첨가로 더 이상의 활성증가를 보이지 않았다. Mn2+이 없는 조건에서 두 arginase의 Km값과 Vmax를 조사한 결과, 380 kD arginase는 22 mM과 1.61 $\mu$mole urea.min-1.mg-1 protein, 그리고 179 kD arginase는 30 mM과 0.79 $\mu$mole urea.min-1.mg-1 protein으로 측정되었다.

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Regression Model for Estimating Biomass of Natural Pinus densifrola Forests in Northeast Area of Mt. Paekdu (백두산 동북부지역 소나무 천연림 biomass 추정모델)

  • 김영환;이돈구;맹헌우
    • Journal of Korea Foresty Energy
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    • v.17 no.1
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    • pp.23-29
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    • 1998
  • This study was carried out to develop the regression model for estimating biomass of natural Pinus densiflora forests by stand density in northeast Chinese area of Mt. Paekdu. Four allometric regression models(W=aD$^b$, W=a(D$^2$H)$^b$. logW=a+b$\cdot$ logD+cD and logW=a+b$\cdot$log(D$^2$H)+c(D$^2$H)) were used to estimate biomass for each of the tree components. The suitable regression model for estimating biomass of stem, bark and whole tree above ground was logW=a+b$\cdot$log(D$^2$H)+c(D$^2$H), and that for biomass of branch, needle and needle area, logW=a+b$\cdot$logD+cD for all of the stand density classes.

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Isotope Effects on Vapor Pressure (증기압에 미치는 동위원소 효과)

  • Youngie Oh;Mu Shik Jhon
    • Journal of the Korean Chemical Society
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    • v.21 no.2
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    • pp.75-82
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    • 1977
  • The isotope effect of pure liquid on vapor pressure is examined, and the vapor pressure ratios of two different isotopes are calculated. Based on the Significant Structure Theory of Liquids, the effect of molecular weight and the effect of moments of inertia are dominant in ordinary/trans-$d_2$ ethylene isotopes; the effect of hindered rotational zero-point energy is dominant in trans-$d_2$/cis-$d_2$ ethylene isotopes; and the effect of intermolecular potential difference is found in $CH_3OH/CH_3OD$ isotopes. In the isotopes of $CH_4/CD_4,\;SiH_4/SiD_4\;and\;GeH_4/GeD_4$, the weight effect decrease in the order of $CH_4/CD_4 >SiH_4/SiD_4 >GeH_4/GeD_4$.

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Antibacterial, Antifungal and Anticonvulsant Evaluation of Novel Newly Synthesized 1-[2-(1H-Tetrazol-5-yl)ethyl]-1H-benzo[d][1,2,3]triazoles

  • Rajasekaran, Aiyalu;Murugesan, Sankaranarayanan;AnandaRajagopal, Kalasalingam
    • Archives of Pharmacal Research
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    • v.29 no.7
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    • pp.535-540
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    • 2006
  • Several novel 1-[2-(1H-tetrazol-5-yl) ethyl]-1H-benzo[d][1,2,3]triazoles (3a-h) have been synthesized by the condensation of 1-[2-(1H-tetrazol-5-yl)-ethyl]-1H-benzotriazole (2) and appropriate acid chlorides. 1-[2-(1H-tetrazol-5-yl)-ethyl]-1H-benzotriazole (2) was synthesized by reacting 3-(1H-benzo[d][1,2,3]triazol-1-yl)propanenitrile with sodium azide and ammonium chloride in the presence of dimethylformamide. The synthesized compounds were characterized by IR and PMR analysis. The titled compounds were evaluated for their in vitro antibacterial and antifungal activity by the cup plate method and anticonvulsant activity evaluated by the maximal electroshock induced convulsion method in mice. All synthesized compounds exhibited moderate antibacterial activity against Bacillus subtilis and moderate antifungal activity against Candida albicans. Compounds 5-(2-(1 H-benzo[d][1,2,3]triazo-1-yl)ethyl)-1H-tetrazol-1-yl)(4-aminophenyl)methanone 3d and 5-(2-(1 H-benzo[d][1,2,3]triazo-1-yl)ethyl)-1H-tetrazol-1-yl)(2-aminophenyl)methanone 3e elicited excellent anticonvulsant activity.

A Characterisitc Analysis Study of Android based Stereoscopic 3D Technology (안드로이드기반 스테레오스코픽 3D 기술 특성분석 연구)

  • Kim, Cheong Ghil
    • Journal of Satellite, Information and Communications
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    • v.8 no.2
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    • pp.68-73
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    • 2013
  • In recent years, the developments in 3D technologies have initiated the commercialization of 3D services on mobile devices. For this purpose, stereoscopic 3D technology is used, which enables 3D TV on mobile devices including smartphone with glasses-free 3D viewing. As a result, the issues related with stereo imaging have been spotlighted greatly. Especially, three MPEG coding standards are provided for mobile 3D services, namely H.264/AVC with and without SEI message and H.264/MVC. In this respect, this paper presents an overview of developments in stereoscopic technologies for mobile devices to gain some perspective on the changes and progress. in this paper, we verified the availability of android based stereoscipic 3D technology related with mobile 3D TV and Smartphone with special emphasis on 3D video format and 3D features by various technollogy characteristics analysis.

Development of OCB mode with impulsive driving scheme for improving moving picture quality

  • Kim, J.L.;Lee, C.H.;Park, S.Y.;Yoo, S.W.;Oh, J.H.;Lee, S.H.;Chai, C.C.;Park, C.W.;Ban, B.S.;Ahn, S.H.;Hong, M.P.;Chung, K.H.;Lim, S.K.;Kim, K.H.;Souk, J.H.
    • 한국정보디스플레이학회:학술대회논문집
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    • 2004.08a
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    • pp.1049-1052
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    • 2004
  • In general, contrary to the CRTs with impulsive emission, liquid crystal displays have motion artifacts such as blurring. ghost image, decrease of dynamic CR(contrast ratio), and stroboscopic motion due to hold type driving method. In this paper, to improve motion picture quality of LCDs. impulsive driving method of black data insertion was applied to the OCB mode which is well known for its fast LC response time and wide viewing angle properties. Subject evaluation was carried out with CRT, TN, SIPS(Super IPS). and impulsive driving OCB. Moving picture image quality near CRT was obtained in impulsive OCB driving mode

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MEMS based micro-fuel processor

  • Kundu, Arunabha;Jang, J.H.;Lee, H.R.;Jung, C.R.;Gil, J.H.;Kim, S.H.;Cha, H.Y.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2006.10a
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    • pp.611-612
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    • 2006
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