• Korean Journal of Veterinary Research
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• v.15 no.1
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• pp.101-108
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• 1975

The susceptibility of 536 isolates of Staphylococcus and 313 isolates of Streptococcus to a number of chemotherapeutics were studied. These organisms were isolated from bovine mastitis during 1973 and 1974. In addition to this, the rate of multiple resistance of 425 isolates of Staphylococcus and 164 isolates of Streptococcus, isolated in 1974, to the chemotherapeutics was analysed. The results obtained in this work were summerized as follows: 1. Staphylococcus and Streptococcus isolated in 1974 showed a higher resistance, with 3 exceptions of chemotherapeutics, than the isolates of 1973. 2. Staphylococcus isolated in 1973 and 1974 showed a higher susceptibility than Streptococcus. 3. The strains of Staphylococcus resistant to colistin were 39 strains (9.2%), to colistin and sulfisoxazole 33 (7.8%), to streptomycin, kanamycin, colistin and sulfisoxazole 20 (4.7%), and to penicillin, colistin and sulfisoxazole 18 (4.2%). 4. The strains of Streptococcus resistant to colistin were 17 strains (10.4%), to streptomycin, kanamycin, colistin and sulfisoxazole 13 (7.9%), to colistin and sulfisoxazole 11 (6.7%) and to penicillin, colistin and sulfisoxazole 11 (6.7%).

• Journal of Applied Biological Chemistry
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• v.64 no.1
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• pp.19-23
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• 2021

Bioactivity-guided fractionation of EtOH extract of the dried fruits of Amomum tsao-ko led to isolation of three compounds (1-3). Their structures were elucidated by spectroscopic methods (MS, 1D and 2D-NMR) and comparison with literature values, as naringenin-5-O-methyl ether (1), helichrysetin (2), and cardamomin (3). Compound 2 was obtained from the genus Amomum for the first time. Among them, compounds 2 and 3 inhibited on the release of β-hexosaminidase from RBL-2H3 cells, with 99.1 and 21.3% at the concentration of 50 μM, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.8
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• pp.952-956
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• 2017

An high performance liquid chromatography (HPLC) analysis method was developed for standard determination of coixol as a functional cosmetic material in Coix lachryma-jobi var. ma-yuen roots extract. HPLC was performed on a $C_{18}$ Unison US column ($4.6{\times}250mm$, $5{\mu}m$ column) using a gradient elution of 0.1% (v/v) trifluoroacetic acid and acetonitrile at a flow rate of 1.0 mL/min at $30^{\circ}C$. The analyte was detected at 290 nm. The HPLC method was validated in accordance with the International Conference on Harmonization guideline of analytical procedures with respect to specificity, precision, accuracy, and linearity. The limit of detection and quantitation were 0.07 and 0.25 mg/mL, respectively. Calibration curves showed good linearity ($R^2$>0.9995), and the precision of analysis was satisfied (less than 0.29%). Recoveries of quantified compounds ranged from 98.36 to 100.30%. This result indicates that the established HPLC method is very useful for the determination of a marker compound in C. lachryma-jobi var. ma-yuen roots extracts.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.45 no.1
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• pp.87-93
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• 2019

This study attempted to eatablish a High Performance Liquid Chromatography (HPLC) analysis method for the determination of (-)-epicatechin gallate as a part of the quality control for the development of functional cosmetic materials from Penthorum chinense Pursh. HPLC was performed on a Unison $US-C_{18}$ column ($4.6{\times}250mm$, $5{\mu}m$) with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and methyl alcohol at a flow rate of 1.0 mL/min at $30^{\circ}C$. The analyte was detected at 280 nm. The HPLC method was performed in accordance with the International Conference on Harmonization (ICH) guideline (version 4, 2005) of analytical procedures with respect to specificity, precision, accuracy, and linearity. The limits of detection and quantitation were 0.11 and 0.33 mg/mL, respectively. Calibration curves showed good linearity ($r^2$ > 0.9999), and the precision of analysis was satisfied (less than 0.6%). Recoveries of quantified compounds ranged from 99.51 to 101.92%. This result indicates that the established HPLC method is very useful for the determination of marker compound in P. chinense Pursh extracts.

• Journal of Mushroom
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• v.19 no.1
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• pp.9-13
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• 2021

This study was performed to find a medium material that can replace Douglas fir sawdust and rice bran in spawn media for growing spawn directly in the oyster mushroom farm. The pH range, total nitrogen source, and total carbon source of the mixed spawn medium were 5.3~5.9, 0.65~1.11%, and 47.0~49.1%, respectively. The C/N ratio was high in the mixed medium of poplar sawdust, with a low total nitrogen content. The protein content was high in the medium containing fermented Douglas fir sawdust. The mycelium growth rate was higher in the medium containing wheat bran than that in the medium containing rice bran. The highest yield per bottle was observed with poplar sawdust, oak sawdust, and rice bran mixed at a ratio of 40:40:20 (v/v/v); however, there was no significant difference observed in terms of productivity with the other treatments. Thus, when growing sawdust spawns in farms, it is efficient to use poplar sawdust, oak sawdust, and wheat bran, which are also easily available, instead of Douglas fir sawdust and rice bran.

• Korean Journal of Veterinary Research
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• v.14 no.2
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• pp.273-279
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• 1974

Studies were made on the relation of mastitis infection rate to the management and sanitations of dairy farms by examining 46 dairy herds. The results obtained were as follows: 1. The higher infection rate was observed in farms managed by employees than by owners, and in impoprted cattle than cattle horn in Korea. 2. Type of education and the period of experience of management were not correlated to the infection rate. 3. The infection rate was the highest in Summer and in cattle of 5~8 lactating age group. 4. It was observed that the amount of water supply, time of cleaning stall, frequency of disinfection, the method of using cleaning towel and the method of milking were correlated to the infection rate.

• Journal of Mushroom
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• v.16 no.2
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• pp.96-102
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• 2018

Sparassis latifolia is one of the most expensive mushrooms in Korean market owing to its high ${\beta}$-glucan content and immunoactivity. However, because of the long cultivation period and high contamination rates, it has low production efficiency. Therefore, we first need to establish the optimum conditions for liquid spawn production to increase its production efficiency. As a result of experiments, molasses culture medium was selected for mycelial growth. Also, the optimum sugar content for molasses and amount of aeration used were approximately 8 Brix% and 0.3~0.6 vvm, respectively. Mycelial dry weight increases, while the medium decreases, as the incubation period increases. Therefore, to achieve maximum production efficiency, the incubation period of 9 to 11 days is appropriate.

• Korean Journal of Food Science and Technology
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• v.37 no.3
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• pp.470-473
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• 2005

Effect of king oyster mushroom (Pleurotus eryngii) on kimchi quality was evaluated during fermentation at $5^{\circ}C$. Kimchi showed rapid decrease in pH up to 7 days, and thereafter slowly decreased. Titratable acidity of kimchi was inversely proportional to pH of kimchi. Treatment of king oyster mushroom did not significantly affect total microbial and lactic acid bacterial counts in kimchi. Antioxidant activity and overall-eating quality of blanched king oyster mushroom-added kimchi were highest among samples tested.

• Journal of the Korean Society for information Management
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• v.30 no.3
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• pp.71-87
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• 2013

In this study, we analyze the agenda setting and policy making process of the establishment of Gyeonggido Cyber Library, which has played an important role for development of public libraries in Gyeonggido since its launching, based on Kingdon's policy streams model. According to the model, policy formation is described as the result from the convergence of the three streams, such as problem, policy and politics streams. When these streams converge on a specific time point, a policy window is created so that the issues become policy agenda. At this moment, policy entrepreneurs propose their alternatives, which have been prepared already, and try to pass it through the window. We identify coupling of the streams in the policy window and the role of policy entrepreneurs in the process of agenda setting and selection of alternatives of Gyeonggido Cyber Library policy. Suggestions are provided based on the analysis for public policy formation in public libraries domain.

• Journal of Digital Convergence
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• v.18 no.1
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• pp.11-21
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• 2020

This paper analyzed the statistical status of public properties, management organization, operational performance focused on Gyeonggido. And This paper tried to get some implications through comparative analysis of public property management system of Gyeonggido and other local governments including Seoul, Incheon. The survey was also conducted on the whole practitioners of public property management in Gyeonggido. As a result of analysis, although Gyeonggido has an urgent need to raise funds because financial independence is insurfficient, non-tax revenues from it's own public properties are insignificant compared to holdings. The major reason would be pointed out that the dedicated management department on public property is not composed and lack of workforce. The survey results conformed that establishment of the dedicated department to public property management is urgent by reorganization of management structure for the higher operational performance.