• 생명과학회지
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• 제18권4호
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• pp.509-514
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• 2008

동 ${\cdot}$ 식물의 추출물은 예로부터 생약으로 많이 이용이 되었는데, 이는 추출물 내에 다양한 생리 활성 물질 및 화합물을 포함 하고 있기 때문이다. 본 연구는 한방에서 누고(樓姑)라고 알려진 땅강아지 추출물에 대한 항산화 활성을 1,1-diphenyl-2-picrylhydrazyl (DPPH)와 ferric reducing ability of plasma (FRAP)활성 실험을 통하여 알아보았다. 땅강아지는 DW, DMSO, 에탄올, 메탄올에 추출하였다. 실험 결과 땅강아지 추출물의 항산화활성은 DPPH radical 소거 활성의 경우 에탄올 추출물에서 가장 높은 활성을 나타났다. FRAP 활성의 경우 DW 추출물에서 가장 높게 나타났으며, 시간에 따른 활성의 증가는 에탄올 추출물에서 가장 높게 나타났다. Cyclooxygenase-2 promoter의 활성 저해는 DMSO와 에탄올 추출물에서 저해활성이 상대적으로 높은 것으로 미루어 보아서 이들 추출물간의 특성을 잘 응용할 경우 항산화 및 항염증에 좋은 생물학적 재료가 될 것으로 판단된다.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권1호
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• pp.127-130
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• 2004

We describe here the cloning and characterization of a cDNA encoding a putative myosin light chain-2 (MLC-2) from the mole cricket, Gryllotalpa orientalis. The G. orientalis MLC-2 cDNA sequences comprised of 615 bp with 205 amino acid residues with a calculated molecular weight of approximately 23 kDa. The deduced protein sequence of G. orientalis MLC-2 cDNA showed 64% and 54% identity to Drosophila melanogaster MLC-2 and D. yakuba MLC-2, respectively. Northern blot analysis confirmed the muscle-specific expression of G. orientalis MLC-2.

• International Journal of Industrial Entomology and Biomaterials
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• 제25권2호
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• pp.175-179
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• 2012

The present study is to develop the rearing method of Gryllotalpa orientalis. In total 429 of G. orientalis were collected from the field rearing cage ($25m^2$) in 2012. Its sex ratio was 1: 1.15(Female : Male). Survival rate of the mole crickets was 94.4~86.1% with the artificial diets formulated for the present study. Successful oviposition rate was 20, 20 and 80% for one, two and three pairs of adult crickets, respectively, from the indoor rearing. The mean number of hatchlings was $11.8{\pm}21.7$, $15.7{\pm}26.4$ and $25.8{\pm}38.8$, and the mean number of dead hatchlings 1.2, 1.7 and 1.2. The mortality of nymphs on horticultural soil and clay sand mixed with ocher was 18.3 and 10.0%, respectively. The mortality of nymphs in circular and rectangular cages was, respectively, 60 and 40%.

• Journal of Ginseng Research
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• 제13권1호
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• pp.119-122
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• 1989

phenological study on the oviposition, emergence, and flight activity of the African mole cricket adult has been made to obtain basic information for management of pest populations in ginseng fields. The flight activity, as monitored by the blarklight trap, seemed to be initiated depending on the sunset time and lasted about 2-2.5 hours. The trap data (1984-1988) showed that the adult flight of the species occurred twice a year, from early May to late June(Spring flight) and from late August to mid October(Fall flight) during which usually more crickets were trapped than during the former period. The number of females trapped was greater than that of males regardless to the flight period, i.e., females comprised 72.2%, 83.9%, and 73.3% of the total catches in 1984, 1985, and 1986, respectively. Adults emerged from late August to mid October and laid eggs from mid May to mid July the next year, indicating that the spring and fall flights correspond to the oviposition and emergence period, respectively.

• 한국응용곤충학회지
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• 제32권1호
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• pp.76-82
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• 1993

경기도 화성군 소재 한국인삼연초연구소 경작시험장 내에서 1990년과 1992년에 땅강아지(Gryllotalpa africana)의 주음성을 조사하였다. 웅성충은 지표 밑 굴의 입구로부터 유인음을 냈은데 강도는 입구 15 cm 위에서 77~80 dB 이었다. 녹음된 웅충 유인음이 직경 1.4m의 funnel중앙에 설치된 2개의 horn speaker에 의해 105~110 dB 로 증폭하여 야외에서 방사되었을 때 비상 성충이 일몰 후 약 30분부터 1.5시간 동안 유인되었다. 유인성충들 중, 14.3~16.7%는 funnel안에, 65.7~74.7%는 발음원으로부터 2 m 이내에 지면에 착지하였다. 자충률은 66.7~74.3%였는데, 이는 야외 개최군의 성비에 기인하는 것으로 보였다. funnel 안과 그 중심에서 2m 이내의 거리에 착지하는 성층수는 blacklight trap에 유인된 성층수보다 다소 많은 경향을 보였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제6권1호
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• pp.87-92
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• 2003

We describe here the cloning, expression and characterization of a cDNA encoding a putative chemosensory protein (CSP) from the mole cricket, Gryllotalpa orientalis. The G. orientalis chemosensory protein cDNA sequences comprised of 384 bp with 128 amino acid residues. The G. orientalis chemosensory protein showed 75.4% protein sequence identity to the Locusta migratoria CSP, Northern blot analysis revealed that signal was stronger in head than leg and cuticle, indicating that the head part containing antennae is a main site for G. orientalis chemosensory protein synthesis. The cDNA encoding G. orientalis chemosensory protein was expressed as approximately 12 kDa polypeptide in baculovirus-infected insect cells.

• International Journal of Industrial Entomology and Biomaterials
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• 제6권2호
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• pp.157-162
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• 2003

The glutathione S-transferase (GSTs) are enzymes responsible for the protection of cells from chemical toxicants and oxidative stress. We describe here the cDNA sequence and mRNA expression of a putative GST from the mole cricket, Gryllotalpa orientalis. The G. orientalis GST cDNA sequences comprised of 621 bp encoding 207 amino acid residues. The multiple sequence alignment of G. orientalis GST gene with other known insect GSTs showed several conserved residues that may be essential for the enzymatic activity of the protein. Phylogenetic analysis of the deduced amino acid sequences of G. orientalis GST gene with other insect GST sequences revealed that the G. orientalis GST gene belongs to class I GST, forming a strong monophyletic group (100% bootstrap value) exclusively for class I GSTs from a diverse insect species. Northern blot analysis confirmed midgut-specific expression at transcriptional level, evidencing the midgut as a site for GST synthesis.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2003년도 제46회 춘계 학술연구 발표회
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• pp.67-67
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• 2003

The NAD$^{+}$-dependent 15-hydroxyprostagladin dehydrogenase (15-PGDH) is a key catabolic enzyme responsible for the control of the biological activities of prostagladins. So far the gene has been found in a diverse organism including three insect dipteran species and one lepidopteran species. In this study, a cDNA encoding the 15-PGDH gene homologue was isolated from the cDNA library of the mole cricket, Gryllotalpa orientalis. (omitted)d)

• 한국응용곤충학회지
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• 제31권4호
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• pp.379-385
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• 1992

땅강아지에 의한 인삼의 피해가 1984~1991년에 야외 및 실내에서 조사되었다. 인삼의 땅강아지 피해는 주로 2년근(2년생) 포장에서 5~6월(춘기)에 발생하였고 성주의 야외 밀도가 높은 9~10월(추기)에는 피해가 거의 인식되지 않았다. 실내 및 강실에서, 2년근의 추기 피해는 뚜렷이 감소하였는데 인삼의 직경과는 무관하였으며, 3년근은 전혀 식해되지 않았다. 토양경도는 땅강아지 성충에 의한 인삼의 피해에 영향을 미치는 것으로 보였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제7권1호
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• pp.37-44
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• 2003

Alcohol dehydrogenases (AHDs) are enzymes responsible for the catalysis of the reversible conversion of various alcohols to their corresponding aldehydes and ketonesis. Until now cDNA sequences of ADH gene is informed exclusively from several diptean species. We describe here the cDNA sequence and mRNA expression of a putative ADH gene from the mole cricket, Gryllotalpa orientalis, and phylogenetic relationships among known insect ADHs. The G. orientalis ADH cDNA sequences comprised of 798 bp encoding 266 amino acid residues. The multiple sequence alignment of G. orientalis ADH gene and known dipteran ADHs shared 100％ identity in the nine amino acid residues that are important for the enzymatic activity in Drosophila melanogaster. Percent sequence identity ranged from 25％ to 32％ among all insect ADHs including both types of ADHs. G. orientalis ADH gene showed no clear resemblance to any dipteran species and type. Phylogenetic analysis of the deduced amino acid sequences of G. orientalis ADH gene with available dipteran ADH genes including both types of ADHs further confirmed that the G. orientalis ADH gene is not clearly assigned to either type of ADHs. Northern blot analysis revealed a stronger signal in the fat body than midgut and epidermis, indicating that the fat body possibly is a main site for the synthesis of the G. orientalis ADH protein.