Molecular Cloning of the Myosin Light Chain-2 cDNA of Gryllotalpa orientalis

  • Cha, So Young (Department of Agricultural Biology, National Institute of Agricultural Science and Technology) ;
  • Hwang, Jae Sam (Department of Agricultural Biology, National Institute of Agricultural Science and Technology) ;
  • Kim, Iksoo (Department of Agricultural Biology, National Institute of Agricultural Science and Technology)
  • Published : 2004.09.01

Abstract

We describe here the cloning and characterization of a cDNA encoding a putative myosin light chain-2 (MLC-2) from the mole cricket, Gryllotalpa orientalis. The G. orientalis MLC-2 cDNA sequences comprised of 615 bp with 205 amino acid residues with a calculated molecular weight of approximately 23 kDa. The deduced protein sequence of G. orientalis MLC-2 cDNA showed 64% and 54% identity to Drosophila melanogaster MLC-2 and D. yakuba MLC-2, respectively. Northern blot analysis confirmed the muscle-specific expression of G. orientalis MLC-2.

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References

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