• Title/Summary/Keyword: Growth promoting

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Breeding and Production Research Direction for Soybean Self-Sufficiency Improvement in Korea

  • Jee-Yeon Ko;Beom-Kyu Kang;Jeong-Hyun Seo;Jun-Hoi Kim;Su-Vin Heo;Man-Soo Choi;Jae-Bok Hwang ;Choon-Song Kim;Myeong-Gyu Oh
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.23-23
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    • 2022
  • Recently, soybean production and market price are unstable, even if demand of soybean is maintained. Diverse conditions such as climate change, a decrease in rural population, and consuming affect food industry. In this situation, food security is soaring as important key-word again, and MAFRA is promoting policies for improving soybean self-sufficiency with the goal of 40% until 2030. The point of policy is to extend a production and stabilize a demand for soybean with supporting large-scale soybean paddy-field complex. According to the background, soybean breeding and production research in NICS are proceeded with three parts. First, production improvement with soybean cultivation land enlargement and high-yield cultivar development. Various growth period soybean cultivars for double cropping, irrigation management technologies in paddy field, and hyper-yield and specific-region adaptable cultivar development. Second, reduction of production expense with mechanized cultivation and digital-based field management technologies. Third, consumer-friendly and high quality soybeans with high protein cultivar for alternative protein usage and high food process-ability for soy milk, tofu, soybean sprouts, and grain usage. Each part need to be combined and advanced to improve soybean industry and soybean self-sufficiency.

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Optimizing hormonal and amino acid combinations for enhanced cell proliferation and cell cycle progression in bovine mammary epithelial cells

  • Hyuk Cheol Kwon;Hyun Su Jung;Do Hyun Kim;Jong Hyeon Han;Seo Gu Han;Dong Hyun Keum;Seong Joon Hong;Sung Gu Han
    • Animal Bioscience
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    • v.36 no.11
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    • pp.1757-1768
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    • 2023
  • Objective: The number of bovine mammary epithelial cells (BMECs) is closely associated with the quantity of milk production in dairy cows; however, the optimal levels and the combined effects of hormones and essential amino acids (EAAs) on cell proliferation are not completely understood. Thus, the purpose of this study was to determine the optimal combination of individual hormones and EAAs for cell proliferation and related signaling pathways in BMECs. Methods: Immortalized BMECs (MAC-T) were treated with six hormones (insulin, cortisol, progesterone, estrone, 17β-estradiol, and epidermal growth factor) and ten EAAs (arginine, histidine, leucine, isoleucine, threonine, tryptophan, lysine, methionine, phenylalanine, and valine) for 24 h. Results: Cells were cultured in a medium containing 10% fetal bovine serum (FBS) as FBS supplemented at a concentration of 10% to 50% showed a comparable increase in cell proliferation rate. The optimized combination of four hormones (insulin, cortisol, progesterone, and 17β-estradiol) and 20% of a mixture of ten EAAs led to the highest cell proliferation rate, which led to a significant increase in cell cycle progression at the S and G2/M phases, in the protein levels of proliferating cell nuclear antigen and cyclin B1, cell nucleus staining, and in cell numbers. Conclusion: The optimal combination of hormones and EAAs increased BMEC proliferation by enhancing cell cycle progression in the S and G/2M phases. Our findings indicate that optimizing hormone and amino acid levels has the potential to enhance milk production, both in cell culture settings by promoting increased cell numbers, and in dairy cows by regulating feed intake.

Empirical Study of Cross-Border E-commerce Brand Formation

  • Jing Zhang;Ziyang Liu
    • Journal of the Korea Society of Computer and Information
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    • v.28 no.11
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    • pp.209-226
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    • 2023
  • Cross-border e-commerce has emerged as a crucial force in stabilizing China's foreign trade, driving the transformation and upgrade of China's foreign trade, and contributing to global economic development and stability. As a result, promoting the high-quality development of cross-border e-commerce has become a common challenge within the Chinese industry. However, with the rapid growth of cross-border e-commerce, it faces new phenomena and challenges, such as a mix of quality, fierce competition, and price competition. Brands serve as distinguishing markers for identifying entities, and brand building is a prudent choice for achieving high-quality development in cross-border e-commerce. This article utilizes brand theory, employing a combined qualitative and quantitative approach, with a specific focus on key elements within the operations of cross-border e-commerce enterprises. Through an analysis of the key elements influencing the formation and development of brands within the internal operations of Chinese cross-border e-commerce enterprises, it investigates their internal formation mechanisms and assesses the significance of these critical factors in influencing brand formation. The research process is based on data from overseas consumers, conducting empirical analysis, ultimately providing certain guidance for brand development in cross-border e-commerce enterprises.

Qualitative Study on Vocational Rehabilitation Program Participation Experience of Mentally Ill Patients in Psychiatric Day Hospital Care (정신과 환자의 낮병원 직업재활 프로그램 참여 경험에 관한 질적 연구)

  • Kwang-Jin Eom;Jung-Yoo Kim
    • Industry Promotion Research
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    • v.8 no.4
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    • pp.209-220
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    • 2023
  • This study used general qualitative research and G-PhotoVoice methods to study the experience of psychiatric patients participating in vocational rehabilitation programs in daytime hospitals. The researcher in this study conducted interviews more than two times with five patients who took part in the program and data was also collected from additional conversations while patients were admitted or on outpatient visit after being discharged from the day hospital. In addition, semi-structured group interview with photos was also conducted. As a result of the study, the participants' experience of participating in the day hospital vocational rehabilitation program was found to be "experiencing difficulties in vocational activities," "joying working together," "getting a sense of stability," and "feeling that they have grown."Based on these findings, this study discussed the implications of activating vocational rehabilitation in daytime hospitals and promoting the growth of psychiatric patients.

Analyzing the Effects of Consumer Value Perception, Environmental Motives, and Perceived Barriers on the Purchase Intention of Vegan Cosmetics (비건 화장품의 구매의도에 영향을 미치는 소비자 가치 인식, 환경적 동기 및 지각된 장벽의 영향 분석)

  • Eun-Hee Lee;Seunghee Bae
    • Journal of the Korean Applied Science and Technology
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    • v.40 no.5
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    • pp.1043-1054
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    • 2023
  • Amidst the rapid growth of the vegan cosmetics market, consumer orientation towards environmental and ethical values has been intensifying. However, research on this subject remains limited. This study delves into the relationship between consumer value perception, environmental motivations, and perceived barriers influencing the purchase intentions of vegan cosmetics. Conducting a PLS-SEM analysis on a sample of 300 women with experience using vegan cosmetics, it was discerned that monetary value, social value, brand value, emotional value, quality value, and environmental knowledge play significant roles in influencing purchase intentions. The moderating effect analysis highlighted image barriers and value barriers as crucial factors. Through Importance-Performance Map Analysis, emotional value emerged as a pivotal element in strategizing to strengthen the purchasing intentions for vegan cosmetics. This research contributes both theoretically and practically to enhancing the competitive edge of the vegan cosmetics market and promoting sustainable consumption behavior.

Proposal for Research Model of Agricultural and Fishery Farm Tower (수직형 농축수산 팜의 연구 모델 제안)

  • Young-Su Lee;Seung-Jung Shin
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.24 no.3
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    • pp.69-76
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    • 2024
  • This dissertation developed a five-story vertical livestock and fisheries farm (palm tower) model for sustainable food production in cities. It proposes to integrate marine farms, livestock raising, and pesticide-free automated crop farms to efficiently use resources and minimize environmental impact. Based on circular economy principles, the model can recycle the output of each part into resources from the other, increasing the efficiency of the system, utilizing idle space in the city, and promoting job creation and community participation. It can also contribute to reducing the carbon footprint of food production and improving food safety. In addition, the study explores how advanced agricultural technologies can be integrated into urban structures to address global food security challenges. This model presents potential solutions to the food crisis caused by climate change and population growth, and suggests a direction for the development of urban agriculture. Future research should address the technical and policy challenges for practical implementation.

A Study on Customer Awareness and a Strategy for Enhancing No-Show Situations at Nail Salons (네일샵 예약부도 고객인식 및 개선방안 연구)

  • Da-Sol Lim
    • Journal of Advanced Technology Convergence
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    • v.3 no.1
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    • pp.43-49
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    • 2024
  • The beauty industry has evolved into a highly competitive service sector, expanding alongside economic growth. Nail art, in particular, has witnessed a remarkable surge in popularity. To efficiently manage individual time, nail salons have adopted reservation systems. However, the issue of no-shows, where customers fail to keep their appointments, has emerged as a significant societal and economic concern. This study seeks to identify and address the root causes of no-shows in nail salons, providing essential data for the industry. A comprehensive survey was conducted, involving 476 customers from nail salons in the Daejeon and Chungcheong regions. Survey responses were analyzed using SPSS 24.0 software. Results revealed that a change of mind was the primary reason for no-shows. This underscores the importance of promoting reservation awareness among both businesses and consumers. To mitigate no-shows, suggestions included implementing non-refundable reservation deposits and imposing cancellation fees. Notably, the beauty industry currently lacks systematic regulations concerning no-shows, highlighting the necessity for future research aimed at developing reservation-related guidelines and standards.

Root Colonization by Beneficial Pseudomonas spp. and Bioassay of Suppression of Fusarium Wilt of Radish (유용 Pseudomonas 종의 근면점유와 무우 Fusarium시들음병의 억제에 관한 생물학적 정량)

  • Lee, Min-Woong
    • The Korean Journal of Mycology
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    • v.25 no.1 s.80
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    • pp.10-19
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    • 1997
  • Fusarium wilt of radish (Raphanus sativus L.) is caused by the Fusarium oxysporum f. sp. raphani (FOR) which mainly attacks Raphanus spp. The pathogen is a soil-borne and forms chlamydospores in infected plant residues in soil. Infected pathogen colonizes the vascular tissue, leading to necrosis of the vascular tissue. Growth promoting beneficial organisms such as Pseudomonas fluorescens WCS374 (strain WCS374), P. putida RE10 (strain RE10) and Pseudomonas sp. EN415 (strain EN415) were used for microorganisms-mediated induction of systemic resistance in radish against Fusarium wilt. In this bioassy, the pathogens and bacteria were treated into soil separately or concurrently, and mixed the bacteria with the different level of combination. Significant suppression of the disease by bacterial treatments was generally observed in pot bioassy. The disease incidence of the control recorded 46.5% in the internal observation and 21.1% in the external observation, respectively. The disease incidence of P. putida RE10 recorded 12.2% in the internal observation and 7.8% in the external observation, respectively. However, the disease incidence of P. fluorescens WCS374 which was proved to be highly suppressive to Fusarium wilt indicated 45.6% in the internal observation and 27.8% in the external observation, respectively. The disease incidence of P. putida RE10 mixed with P. fluorescens WCS374 or Pseudomonas sp. EN415 was in the range of 10.0-22.1%. On the other hand, the disease incidence of P. putida RE10 mixed with Pseudomonas sp. EN415 was in the range of 7.8-20.2%. The colonization by FOR was observed in the range of $2.4-5.1{\times}10^3/g$ on the root surface and $0.7-1.3{\times}10^3/g$ in the soil, but the numbers were not statistically different. As compared with $3.8{\times}10^3/g$ root of the control, the colonization of infested ROR indicated $2.9{\times}10^3/g$ root in separate treatments of P. putida RE10, and less than $3.8{\times}10^3/g$ root of the control. Also, the colonization of FOR recorded $5.1{\times}10^3/g$ root in mixed treatments of 3 bacterial strains such as P. putida RE10, P. fluorescens WCS374 and Pseudomonas sp. EN415. The colonization of FOR in soil was less than that of FOR in root part. Based on soil or root part, the colonization of ROR didn't indicate a significant difference. The colonization of introduced 3 fluorescent pseudomonads was observed in the range of $2.3-4.0{\times}10^7/g$ in the root surface and $0.9-1.8{\times}10^7/g$ in soil, but the bacterial densities were significantly different. When growth promoting organisms were introduced into the soil, the population of Pseudomonas sp. in the root part treated with P. putida RE10 was similar in number to the control and recorded the low numerical value as compared with any other treatments. The population density of Pseudomonas sp. in the treatment of P. putida RE10 indicated significant differences in the root part, but didn't show significant differences in soil. The population densities of infested FOR and introduced bacteria on the root were high in contrast to those of soil. P. putida RE10 and Pseudomonas sp. EN415 used in this experiment appeared to induce the resistance of the host against Fusarium wilt.

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The Outcome of the 6th ICAO Worldwide Air Transport Conference and Fair Competition Policy in International Air Transport (국제항공운송의 최근 동향과 항공운송의 공정경쟁정책 -ICAO 제6차 세계항공운송회의 결과를 중심으로-)

  • Shin, Dong-Chun
    • The Korean Journal of Air & Space Law and Policy
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    • v.28 no.1
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    • pp.97-114
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    • 2013
  • The 6th Worldwide Air Transport Conference was held in Montreal in March 2013 under the auspices of ICAO. This conference, which has been held every ten years, is dealing with virtually every issue of international air transport, and aiming at updating ICAO policy in order to ensure long-term growth of international civil aviation. Last conference which took place in 2003 focused on the liberalization of air transport, and the 6th conference shifted its focus from whether to push for liberalization, to how to implement it. The main agenda items for the 6th conference was liberalization, safeguards, ownership, fair competition, airports and air navigation facilities, charges, and ICAO policy. The liberalization, and in particular progressive liberalization has been a main theme over the past decades. In the process leading to liberalization, there needs to be the expansion of market access, easing regulation on ownership and control of airlines. Furthermore, the provision of enough infrastructure such as airport and air navigation facilities may be contributing factor to remove impediments to liberalization. However, out of concern as for undermining interests of consumer and the weak, when liberalization is proceeding in a sudden and radical manner, there should be safeguards so as to ensure market participation by developing countries, consumer protection, and economical and transparent decision on taxes and charges. Fair competition which differs from promoting competition in the market, is a policy in order to protect the weak players and consumers from monopoly and oligopoly. The Korean delegation submitted 3 WPs (WP/85, 86 and 87) and 1 IP, and presented WPs, at the conference, which were a lot compared with previous occasions. A paradigm shift was emphasized to expedite the process of liberalization at the 6th conference. The reality is that with many previous recommendations to stress the importance of liberalization, and to urge States to change their attitudes, the pace of the liberalization has been very slow and staggering. The liberalization of air transport will contribute to the growth of air transport and related industry, to create new employment, promoting tourism and regional development, and further to facilitating mutual understanding and exchange, which will also lead to making a barrier-free world. In this context, it is expected that the next conference will also evaluate the on-going process of liberalization.

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Cloning of the Cellulase Gene and Characterization of the Enzyme from a Plant Growth Promoting Rhizobacterium, Bacillus licheniformis K11 (고추역병 방제능이 있는 식물성장촉진 균주 Bacillus licheniformis K11의 cellulase 유전자의 cloning 및 효소 특성 조사)

  • Woo, Sang-Min;Kim, Sang-Dal
    • Applied Biological Chemistry
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    • v.50 no.2
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    • pp.95-100
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    • 2007
  • The cellulase gene of Bacillus licheniformis K11 which has plant growth-promoting activity by auxin and antagonistic ability by siderophore was cloned in pUC18 using PCR employing heterologous primers. The 1.6kb PCR fragment contained the full sequence of the cellulase gene, denoted celW which has been reported to encode a 499 amino acid protein. Similarity search in protein data base revealed that the cellulase from B. licheniformis K11 was more than 97% identical in amino acid sequence to those of various Bacillus spp. The cellulase protein from B. licheniformis K11, overproduced in E. coli DH5${\alpha}$ by the lac promoter on the vector, had apparent molecular weight of 55 kDa upon CMC-SDS-PAGE analysis. The protein not only had enzymatic activity toward carboxymethyl-cellulose (CMC), but also was able to degrade insoluble cellulose, such as Avicel and filter paper (Whatman$^{\circledR}$ No. 1). In addition, the cellulase could degrade a fungal cell wall of Phytophthora capsici. Consequently B. licheniformis K11 was able to suppress the peperblight causing P. capsici by its cellulase. Biochemical analysis showed that the enzyme had a maximum activity at 60$^{\circ}C$ and pH 6.0. Also, the enzyme activity was activated by Co$^{2+}$ of Mn$^{2+}$ but inhibited by Fe$^{3+}$ or Hg$^{2+}$. Moreover, enzyme activity was not inhibited by SDS or sodium azide.