• Journal of The Korean Society of Agricultural Engineers
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• v.58 no.6
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• pp.93-100
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• 2016

This study conducted a quantitative assessment on the environmental flows associated with climate change in the Gosam Reservoir, Korea. The application of RCP 8.5 climate change scenario has found that the peak value of High Flow Pulses has increased by 36.0 % on average compared to historical data (2001 ~ 2010), which is likely to cause disadvantage on flood control and management but the increase in peak value is expected to make a positive impact on resolving the issue of green algal blooms, promoting vegetation in surrounding areas and encouraging spawning and providing habitats for native species by releasing a larger amount of landslides as well as organic matters than the past. However, the decreasing pattern of the peak value of High Flow Pulses is quite apparent with the trend of delay on the occurrence time of peak value, necessitating a long-term impact analysis. The peak value of Large Floods shows a clear sign of decrease against climate change scenario, which is expected to lead to changes in fish species caused by degraded quality of water and decreasing habitats. A quicker occurrence of Small Floods is also expected to make an impact on the growth cycle of aquatic plants, and the reduction in occurrence frequency of Extreme Low Flows is to contribute to increasing the population of and raising the survival rate of native fish, greatly improving the aquatic ecosystem. The results of this study are expected to be useful to establish the water environment and ecological system in adapting or responding to climate change.

• Food Science and Biotechnology
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• v.16 no.5
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• pp.843-847
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• 2007

Strain NK34 was characterized for probiotic use. Strain NK34 was named Lactococcus lactis NK34 based on API 50 CHL kit results and 16S rDNA sequencing. L. lactis NK34 was highly resistant to artificial gastric juice (pH 2.5) and artificial bile acid. Based on results from the API ZYM kit, 4 enzymes were produced. L. lactis NK34 was resistant to all antibiotics tested except for $10\;{\mu}g/mL$ roxithromycin and $10\;{\mu}g/mL$ erythromycin. The cholesterol-lowering effect of L. lactis NK34 was about 46.9%. Concentrations of interleukin $(IL)-1{\alpha}$ in the $20{\times}$ concentrated supernatant of L. lactis NK34 was about 361 pg/mL. L. lactis NK34 was also found to inhibit the growth of colon cancer cells due to MNNG-induced DNA damage. These results demonstrate the potential of L. lactis NK34 as a health-promoting probiotic.

• Journal of Microbiology and Biotechnology
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• v.11 no.6
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• pp.986-993
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• 2001

Translation Initiation in prokaryotes involves the formation of a 30 S preinitiation complex, in which translation initiation factors play a role in the stimulation of fMet-tRNA (fMet) binding. However, the specific function and precise mechanism of initiation factor IF1 are still unclear. One a functionally active factor with a high purity. In the present study a large quantity of active IF was rapidly purified, obtained by the overexpression of the infA gene, and then used for a functional study. The induction of infA did not appreciably affect the growth rate of the protease-deficient strain E. coli AR68 harboring the IF1 overproducing plasmid. The level of IF1 obtained was approximately $1-2\%$ of the total cell protein, which enabled the yield of highly purified IF1 (>$98\%$ pure) to be increased to 0.15 mg of IF1/g of cells. The IF1 was isolated within one day by the centrifugatioin of the ribosomal washed fraction, by ammonium sulfate fractionation, chromatography on batch of phosphocellulose, and FPLC Mono S. The overexpressed IF1 was found to be comparable to the factor isolated from normal cells, as determined by migration in NEPHGE/SDS 2-D gels. For binding of fMet-tRNA(fMet) to the 30 S ribosomal subunitis, relatively high levels of binding were obtained when IF2 was present. The addition of IF1 up to 110 pmol proportionally stimulated the binding to a variable extent. This IF1-dependent stimulation of the 30 S preinitiation complex formation demonstrated that IF1 would appear to be exclusively essential for promoting the initiation phase of protein synthesis.

• Journal of Microbiology and Biotechnology
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• v.15 no.5
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• pp.1001-1010
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• 2005

Bacillus megaterium KL39, an antibiotic-producing plant growth promoting rhizobacterium (PGPR), was selected from soil. The antifungal antibiotic, denoted KL39, was purified from culture filtrate by column chromatography using Dion HP-20, Silica gel, Sephadex LH-20, and prep-HPLC. Thin layer chromatography, employing the solvent system of ethanol:ammonia:water=8:1:1, showed the $R_{f}$. value of 0.32. The antibiotic KL39 showed a negative reaction with ninhydrin solution, positive with iodine vapor, and also positive with Ehrlich reagent. It was soluble in methanol, ethanol, butanol, and acetonitrile, but insoluble in chloroform, toluene, hexane, ethyl ether, or acetone. Its UV spectrum had the maximum absorption at 208 nm. Amino acid composition, FAB-mass, $^{1}H-NMR,\;^{13}C-NMR$, and atomic analyses showed that the antibiotic KL39 (MW=1,071) has a structure very similar to iturin E. The antibiotic KL39 has a broad antifungal spectrum against a variety of plant pathogenic fungi including Rhizoctonia solani, Pyricularia oryzae, Monilinia froeticola, Botrytis cinenea, Altenaria kikuchiana, Fusarium oxysporum, and F. solani. An MIC value of $10\;{\mu}g/ml$ was determined for Phytophthora capsici. Macromolecular incorporation studies with P. capsici using radioactive [$^{3}H-adenine$] as the precursor, indicated that the antibiotic KL39 strongly inhibits the DNA biosynthesis of the fungal cell. Microscopic observation of the antifungal action showed abnormal hyphal swelling of P. capsici. The purified antibiotic KL39 was very effective for the biocontrol of in vivo Phytophthora-blight disease of pepper.

• Journal of the Korean association of regional geographers
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• v.13 no.5
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• pp.477-494
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• 2007

This paper offers a brief overview of the historical implications of residential segregation in Busan, Korea. It first reviews the history of residence from the early 1900s to the present and the transition of residential boundary of the City of Busan. It then considers the characteristics of residential segregation and the important factors in it. The social class of pre-industrial society led the inequality of residential areas around the Eubchee. The existed Korean residential areas were separated by newly built-up residential areas in the Japanese colonial era. After the liberation from Japanese Rule and Korean War, rapid population growth made considerable slums across the City of Busan. These slums were an important factor to segregate residential areas from prosperous areas ever afterward. The socio-economic status has related to residential segregation through the formation of differential housing classes since 1990s. The historial analysis of these periods will offer an insight into how the dominant factors lead residential segregation in a specific period by promoting a more socio-economically integrated society.

• Journal of Biosystems Engineering
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• v.7 no.2
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• pp.36-44
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• 1983

Farm population was rapidly decreasing due to shift of the people from farm sector to the non-farm sector caused by the economic growth of the country. Especially, a great shortage of farm labor in busy farming period in June and October is becoming a serious problem in maintaining or promoting land productivity. The peak of labor requirement in summer is caused by rice transplanting and barley harvesting. In order to reduce the restrictions imposed on farm management by the concurrence of labor requirement and the lack of labor, the experimental study for mechanization of barley harvesting has been carried out in the fields. 1. The machines for barley harvesting were knap-sack type reapers, windrow reaper (power tiller attachment), binder and combine. The order of higher efficiency of machine for barley harvesting was combine, binder, windrow reaper (WR), knapsack type reaper 1(KSTR1), and knap sack type reaper 2(KSTR2; mist and duster attachment). 2. The ratio of grain loss for the manual, binder, and combine plot was about four percent of total field yield. 3. The total yield of barley in 35 days and 40 days harvesting after heading were 514 kg and 507kg per 10 ares respectively. The yield of 35 days-plot was higher than other experimental plots. 4. The lowest yield was recorded in 30 days-plot due to the large quantity of immatured grains and having lighter 1000-grain weight. The ratio of immatured grains was 2.66 percent and 1000-grain weight was 29.4 grams. 5. The total harvesting cost of the windrow reaper was 10,178 won per 10 ares. It was the lowest value compared to other machines. The next were combine, binder, KSTR1, KSTR2, and manual in sequence. As a result, the optimum time of barley harvesting for mechanization was 35-40 days after heading. Combine, binder, and windrow reaper were recommended as the suitable machines for barley harvesting in the work efficiency. However, in total harvesting cost, the windrow reaper was the most promising machine for barley harvesting.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.6
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• pp.2823-2829
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• 2011

All of the government, private enterprises, and local governments are promoting the spread of electric vehicles to reduce carbon emissions depending on the green growth policy of the government that began in 2009. Due to this background, the prevalence of electric cars is being spread. However, the efficient management and operation through the exchanging information between electric vehicles and charging infrastructure, as well as the development of electric vehicles are essential to disseminate them. In this paper, we present a service framework for exchange of information between electric vehicles and charging infrastructure, and propose a communication method to meet it. To do this, we propose the most appropriate communication method through the performance evaluation by identifying and comparing the existing wireless communication methods can be applied to exchange information between electronic vehicles and EV charging infrastructure.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.6
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• pp.2397-2402
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• 2015

Previous studies have shown that miR-454 plays an important role in a variety of biological processes in various human cancer cells. However, the underlying mechanisms of this microRNA in colorectal cancer (CRC) cells remain largely unknown. In the present study, we investigated the miR-454 role in CRC cell proliferation. We found that miR-454 expression is markedly upregulated in CRC tissues and CRC cells compared with the matched tumor adjacent tissues and the FHC normal colonic cell line. Ectopic expression of miR-454 promoted the proliferation and anchorage-independent growth of CRC cells, whereas inhibition of miR-454 reduced this effect. Bioinformatics analysis further revealed cylindromatosis (CYLD), a putative tumor suppressor as a potential target of miR-454. Data from luciferase reporter assays showed that miR-454 directly binds to the 3'-untranslated region (3'-UTR) of CYLD mRNA and repressed expression at both transcriptional and translational levels. In functional assays, CYLD-silenced in miR-454-in-transfected SW480 cells have positive effect to promote cell proliferation, suggesting that direct CYLD downregulation is required for miR-454-induced CRC cell proliferation. In sum, our data provide compelling evidence that miR-454 functions as an onco-miRNA, playing a crucial role in the promoting cell proliferation in CRC, and its oncogenic effect is mediated chiefly through direct suppression of CYLD expression.

• The Journal of the Korea Contents Association
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• v.14 no.6
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• pp.73-83
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• 2014

This paper is to analyse how Industree Crafts, social enterprise in the field of crafts, could generate profit and create a sustainable growth for the last 30 years out of dependence on government grants in India. Success factors was drawn from the findings as follows. First, bringing stakeholder value: it preferred social mission as a goal for generating the value of stakeholders respectively and shared value mutually. Second, building distinctive internal business processes: it adapted unique business model and 4P strategy into its internal organization system and introduced the way of management to support the independence of self-help groups aimed at creating greater added value. Third, promoting learning and innovation culture: it challenged with the help of new design strategies continuously, was unafraid of change, and promoted learning culture to turn trial and error into the organization learning. Finally fourth, securing right finances and resources: it obtained proper finances and resources such as trying joint-sale, attracting investment from a private company, and acquiring enough skilled artisans at every scaling up.

• Journal of Korea Technology Innovation Society
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• v.17 no.1
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• pp.1-24
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• 2014

The new Korean government sets its main policy direction as Creative Economy. The Creative Economy can be defined as a growth strategy that establishes new companies, enhances the competitiveness of established companies and creates new markets, industries and jobs in consequence of combining of creative imagination, science and technology and ICT. In this context, the concept of innovation cluster, which aims to foster technological innovations from an organic ecosystem perspective, can be a valid policy instrument for attaining Creative Economy. Innovation cluster can and must make a contribution to achieving the Creative Economy of Korea. In particular, the ICT Convergence Innovation Cluster will be a good strategy for this purpose. Based on this conceptual background, this paper analyzes a recently notable case of an ICT Convergence Cluster in Korea, Pangyo Technovalley (PTV). We argue that the Pangyo Technovalley is a representative cluster of converging ICT and science and technology. We identify the major factors for successful development of ICT cluster: regional government's flexible response to environmental change, careful pre-planning and balance between private and public involvement. In addition, we also found that additional factors, settlement condition for securing good workforce, agglomeration of various innovation actors for promoting convergence, and cluster network revitalization are also important for implementing the creative ICT convergence cluster.