• Journal of Microbiology and Biotechnology
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• 제30권7호
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• pp.1018-1026
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• 2020

Rosemary essential oil was evaluated for antifungal potentiality against six major ginseng pathogens: Sclerotinia sclerotiorum, Sclerotinia nivalis, Cylindrocarpon destructans, Alternaria panax, Botrytis cinerea, and Fusarium oxysporum. The in vitro fungicidal effects of two commonly used fungicides, namely mancozeb and fenhexamid, and the volatile organic compounds (VOCs) of Trichoderma koningiopsis T-403 on the mycelial growth were investigated. The results showed that rosemary essential oil is active against all of the pathogenic strains of ginseng root rot, whereas rosemary oil displayed high ability to inhibit the Sclerotinia spp. growth. The highest sensitivity was S. nivalis, with complete inhibition of growth at 0.1% v/v of rosemary oil, followed by Alternaria panax, which exhibited 100% inhibition at 0.3% v/v of the oil. Minimum inhibitory concentrations (MICs) of rosemary oil ranged from 0.1 % to 0.5 % (v/v). Chemical analysis using GC-MS showed the presence of thirty-two constituents within rosemary oil from R. officinals L. Camphore type is the most frequent sesquiterpene in rosemary oil composition. Mancozeb and fenhexamid showed their highest inhibition effect (45% and 30%, respectively) against A. panax. T. koningiopsis T-403 showed its highest inhibition effect (84%) against C. destructans isolate. This study may expedite the application of antifungal natural substances from rosemary and Trichoderma in the prevention and control of phytopathogenic strains in ginseng root infections.

• The Plant Pathology Journal
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• 제40권4호
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• pp.346-357
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• 2024

This study was carried out to screen the antifungal activity against Colletotrichum acutatum, Colletotrichum dematium, and Colletotrichum coccodes. Bacterial isolate GP-P8 from pepper soil was found to be effective against the tested pathogens with an average inhibition rate of 70.7% in in vitro dual culture assays. 16S rRNA gene sequencing analysis result showed that the effective bacterial isolate as Bacillus siamensis. Biochemical characterization of GP-P8 was also performed. According to the results, protease and cellulose, siderophore production, phosphate solubilization, starch hydrolysis, and indole-3-acetic acid production were shown by the GP-P8. Using specific primers, genes involved in the production of antibiotics, such as iturin, fengycin, difficidin, bacilysin, bacillibactin, surfactin, macrolactin, and bacillaene were also detected in B. siamensis GP-P8. Identification and analysis of volatile organic compounds through solid phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS) revealed that acetoin and 2,3-butanediol were produced by isolate GP-P8. In vivo tests showed that GP-P8 significantly reduced the anthracnose disease caused by C. acutatum, and enhanced the growth of pepper plant. Reverse transcription polymerase chain reaction analysis of pepper fruits revealed that GP-P8 treated pepper plants showed increased expression of immune genes such as CaPR1, CaPR4, CaNPR1, CaMAPK4, CaJA2, and CaERF53. These results strongly suggest that GP-P8 could be a promising biocontrol agent against pepper anthracnose disease and possibly a pepper plant growth-promoting agent.

• 한국재료학회지
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• 제24권12호
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• pp.704-709
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• 2014

In this study, we fabricated a novel micro porous hybrid scaffold of biphasic calcium phosphate (BCP) and a polylectrolyte complex (PEC) of chitosan (CS) and hyaluronic acid (HA). The fabrication process included loading of CS-HA PEC in a bare BCP scaffold followed by lypophilization. SEM observation and porosimetry revealed that the scaffold was full of micro and macro pores with total porosity of more than 60 % and pore size in the range of $20{\sim}200{\mu}m$. The composite scaffold was mechanically stronger than the bare BCP scaffold and was significantly stronger than the CS-HA PEC polymer scaffold. Bone morphogenetic growth factor (BMP-2) was immobilized in CS-HA PEC in order to integrate the osteoinductive potentiality required for osteogenesis. The BCP frame, prepared by sponge replica, worked as a physical barrier that prolonged the BMP-2 release significantly. The preliminary biocompatibility data show improved biological performance of the BMP-2 immobilized hybrid scaffold in the presence of rabbit bone marrow stem cells (rBMSC).

• 유통과학연구
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• 제15권2호
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• pp.53-58
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• 2017

Purpose - The world economy is changing with FTA. Lots of FTAs are going on between countries and economic blocs in the world economy as the battle field of FTA. This study is aims to suggest a practical data about Korea-China FTA by analyzing an economic ripple effect and main issues on Korea-China FTA negotiation. Research design, data, and methodology - This study analyzes the economic impact and major issues expected during the promotion period of Korea-China FTA, and promoted the purpose of this research with literature review and comparative analysis. Result - FTA agreement with China is expected to contribute to Korean economic growth by bringing effectiveness of securing and resolving mutual trade conflict, and one-sided trade protection control of Chinese domestic demand market. The potentiality between United States and Korea is also very important issue. Conclusions - To minimize the adverse effects of FTA and to maximize the positive effects on the logistics industry, the Correspondence strategy is suggested based on the effects of FTA after analyzing the entire situation of the logistics. Especially, this article places emphasis on a close cooperation system between the government agencies to get a good conclusion from the negotiation even if the e-Commerce issue to be a critical point under Korea-China FTA.

• 한국비블리아학회지
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• 제4권1호
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• pp.129-142
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• 1980

The present paper discusses some problem areas in supplying information. As the world advances in civilization, we need exact and rapid information exchange, it may be said in this connection that we also feel keenly the necessity of the industrialization of the information. In accordance with the industrialization of the information, we can find the following phenomena: 1. Increase of present potentiality users. 2. Demand of high level information in accordance with the improvement of education level, 3. Increase of demand in the information materials in accordance with growth of research and development. 4. Contraction of the existing knowhow and degeneration of special field technique, 5. Increase of information concerned person for policy making in the government and in the industry. In the meantime, the information problem occurs for efficient management of information materials, in addition to numerical increase of various kinds, increase of users crate control and standardization problem, so it is needed to improve a mode of expression, structure, file and methods of usage. The librarian, as a information processor, should recognize that the information is national resources and improve the marketability of information industry. We need an administrative system of national level for the development of library in accordance with increase of an administrator who is engaged in information activities.

• 한국관광식음료학회지:관광식음료경영연구
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• 제15권2호
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• pp.1-22
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• 2004

This study was intended to analysis about quantity and quality aspects of speciality coffee industry in korea. All date was analysed by statistics package program spss 10.0. All market of coffee In korea, the market share of speciailty coffee have been maintained about 10 percent since it was analysed statically about coffee market in korea. The rest market is soluble coffee market. But by comparison with developed country case, korea's coffee market is being unbalanced between soluble coffee and speciality coffee market. This study was analyze actual consumer's consulnption styles of the speciality coffee as well as its industry. According to the qualitative analysis results from peter's model was analysed that made a high growth potentiality and profitability in the future. According to the quantitative analysis results, sampling unit's 87.6 percent made a recognize about the speciality coffee. Sensory evaluation results was researched that factors impacted on the overall speciality coffee quality was shown a significant diffrrence on the two dimension such as the body factor, acidity factor. This study has limit which can not represent overall recognition because of analysis based on limited data. It is thought that steady study should be continued hereafter through more systematic and reliable study method.

• Mycobiology
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• 제35권2호
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• pp.100-102
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• 2007

Pet dogs have been considered to be involved in the contamination of indoor air by serving as a source of providing molds at houses. Currently, information on the molds originated from pet dogs is rarely available in Korea. The present study was carried out to obtain basic information on the fungi present on pet dogs. For this, fungal isolation was performed to the skin and hairs of 70 pet dogs at different houses and veterinary hospitals. A total of 44 fungal isolates were obtained from skin (27 isolates) and hairs (17 isolates) of the dogs investigated. Based on the observation of microstructures and colony morphology, and the ITS rDNA sequence analysis, the fungal isolates were identified at the level of genus. The identified isolates belong to the genera of Alternaria, Aspergillus, Beauveria, Chrysosporium, Cladosporium, Penicillium, Scopulariopsis, and Trichoderma. Among these genera, Aspergillus (25%), Cladosporium (23%) and Penicillium (20.5%) were 3 major genera. 63% of the 44 isolates showed color changes on dermatophyte test medium (DTM). When we tested the growth ability of 44 isolates at $37^{\circ}C$, 45% of the isolates were able to grow. These results show that pet dogs could carry fungi having a potentiality of affecting on human health.

• 한국토양비료학회지
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• 제49권4호
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• pp.355-367
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• 2016

Soil salinization refers to the buildup of salts in soil to a level toxic to plants. The major factors that contribute to soil salinity are the quality, the amount and the type of irrigation water used. The presented review discusses the different sources and causes of soil salinity. The effect of soil salinity on biological processes of plants is also discussed in detail. This is followed by a debate on the influence of salt on the nutrient uptake and growth of plants. Salinity decreases the soil osmotic potential and hinders water uptake by the plants. Soil salinity affects the plants K uptake, which plays a critical role in plant metabolism due to the high concentration of soluble sodium ($Na^+$) ions. Visual symptoms that appear in the plants as a result of salinity include stunted plant growth, marginal leaf necrosis and fruit distortions. Different strategies to ameliorate salt stress globally include breeding of salt tolerant cultivars, irrigation to leach excessive salt to improve soil physical and chemical properties. As part of an ecofriendly means to alleviate salt stress and an increasing considerable attention on this area, the review then focuses on the different plant growth promoting bacteria (PGPB) mediated mechanisms with a special emphasis on ACC deaminase producing bacteria. The various strategies adopted by PGPB to alleviate various stresses in plants include the production of different osmolytes, stress related phytohormones and production of molecules related to stress signaling such as bacterial 1-aminocyclopropane-1-carboxylate (ACC) derivatives. The use of PGPB with ACC deaminase producing trait could be effective in promoting plant growth in agricultural areas affected by different stresses including salt stress. Finally, the review ends with a discussion on the various PGPB activities and the potentiality of facultative halophilic/halotolerant PGPB in alleviating salt stress.

• The Journal of Advanced Prosthodontics
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• 제6권5호
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• pp.379-386
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• 2014

PURPOSE. These days, mesenchymal stem cells (MSCs) have received worldwide attention because of their potentiality in tissue engineering for implant dentistry. The purpose of this study was to evaluate various growth inducing factors in media for improvement of acquisition of bone marrow mesenchymal stem cells (BMMSCs) and colony forming unit-fibroblast (CFU-F). MATERIALS AND METHODS. The mouse BMMSCs were freshly obtained from female C3H mouse femur and tibia. The cells seeded at the density of $10^6$/dish in media supplemented with different density of fetal bovine serum (FBS), $1{\alpha}$, 25-dihydroxyvitamin (VD3) and recombinant human epidermal growth factor (rhEGF). After 14 days, CFU-F assay was conducted to analyze the cell attachment and proliferation, and moreover for VD3, the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay was additionally conducted. RESULTS. The cell proliferation was increased with the increase of FBS concentration (P<.05). The cell proliferation was highest at the density of 20 ng/mL rhEGF compared with 0 ng/mL and 200 ng/mL rhEGF (P<.05). For VD3, although the colony number was increased with the increase of its concentration, the difference was not statistically significant (P>.05). CONCLUTION. FBS played the main role in cell attachment and growth, and the growth factor like rhEGF played the additional effect. However, VD3 did not have much efficacy compare with the other two factors. Improvement of the conditions could be adopted to acquire more functional MSCs to apply into bony defect around implants easily.

• 농업생명과학연구
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• 제46권1호
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• pp.207-215
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• 2012

본 연구는 울산 지역에서 성장 잠재력이 높은 농작물을 선정하고자 농작물의 재배면적 및 농업생산액에 대한 2000, 2005 및 2007년의 공식통계자료와 행정자료를 사용하여 특화계수분석과 지역성장률시차 분석을 하였다. 재배면적과 농업생산액에 의한 특화계수분석에 따르면, 쌀, 배, 감 등은 모든 분석시점에서 특화작목으로 분류되고 있다. 이들 작목들은 지역 내에서 차지하는 비중이 상대적으로 높아서 중요하기 때문에 지역농업인들의 영농활동지원 차원에서 단기적인 정책에 반영하는 것이 유용할 것이다. 2000~2007년의 농업생산액을 기준으로 한 지역성장률시차분석결과에 의하면, 딸기, 감, 감자, 옥수수, 고구마 등이 성장작목으로, 배가 경쟁우위작목으로 분류되고 있다. 재배면적 기준에서는, 딸기, 감자, 옥수수 등이 성장작목으로, 고구마가 경쟁우위작목으로 분류되었다. 이들 작목은 시장의 선호도와 생산자의 의지를 반영한 전국적인 추세와 지역의 생산 활동을 고려한 시계열 분석이라는 차원에서 지방자치단체는 중장기 농업정책 수립에 활용하는 것이 유익할 것이다.