Korean Journal of Materials Research (한국재료학회지)

Materials Research Society of Korea (한국재료학회)
권호 표지
DOI QR코드

DOI QR Code

BMP-2 Immoblized in BCP-Chitosan-Hyaluronic Acid Hybrid Scaffold for Bone Tissue Engineering

  • Received : 2014.10.30
  • Accepted : 2014.12.02
  • Published : 2014.12.27
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we fabricated a novel micro porous hybrid scaffold of biphasic calcium phosphate (BCP) and a polylectrolyte complex (PEC) of chitosan (CS) and hyaluronic acid (HA). The fabrication process included loading of CS-HA PEC in a bare BCP scaffold followed by lypophilization. SEM observation and porosimetry revealed that the scaffold was full of micro and macro pores with total porosity of more than 60 % and pore size in the range of $20{\sim}200{\mu}m$. The composite scaffold was mechanically stronger than the bare BCP scaffold and was significantly stronger than the CS-HA PEC polymer scaffold. Bone morphogenetic growth factor (BMP-2) was immobilized in CS-HA PEC in order to integrate the osteoinductive potentiality required for osteogenesis. The BCP frame, prepared by sponge replica, worked as a physical barrier that prolonged the BMP-2 release significantly. The preliminary biocompatibility data show improved biological performance of the BMP-2 immobilized hybrid scaffold in the presence of rabbit bone marrow stem cells (rBMSC).

Keywords

References

  1. M. M. Stevens, Mater. Today, 11(5), 18 (2008).
  2. Hollister, Scott J. "Porous scaffold design for tissue engineering." Nat. Mater., 4(7), 518 (2005). https://doi.org/10.1038/nmat1421
  3. E. S. Place, N. D. Evans, and M. M. Stevens, Nat. Mater. 8(6), 457 (2009). https://doi.org/10.1038/nmat2441
  4. K. J. L. Burg, S. Porter, J. F. Kellam Biomater., 21(23), 2347 (2000). https://doi.org/10.1016/S0142-9612(00)00102-2
  5. M. J. Olszta, X. Cheng, S. S. Jee, R. Kumar, Y. Y. Kim, M. J. Kaufman, E. P. Douglas, and L. B. Gower, Mater Sci Eng: R: Reports, 58(3), 77 (2007). https://doi.org/10.1016/j.mser.2007.05.001
  6. S. Deville, E. Saiz, and A. P. Tomsia, Biomater., 27(32), 5480 (2006). https://doi.org/10.1016/j.biomaterials.2006.06.028
  7. K. F. Leong, C. M. Cheah, and C. K. Chua. Biomater, 24(13), 2363 (2003). https://doi.org/10.1016/S0142-9612(03)00030-9
  8. Q. Hou, D. W. Grijpma, and J. Feijen, Biomater,. 24(11), 1937 (2003). https://doi.org/10.1016/S0142-9612(02)00562-8
  9. M. Vallet-Regi and J. M. Gonzalez-Calbet, Prog Solid State Chem, 32(1), 1 (2004). https://doi.org/10.1016/j.progsolidstchem.2004.07.001
  10. S. I. Roohani-Esfahani, C. R. Dunstan, J. J. Li, Z. Lu, B. Davies, S. Pearce, J. Field, R. Williams, and H. Zreiqat, Acta biomater., 9(6), 7014 (2013). https://doi.org/10.1016/j.actbio.2013.02.039
  11. K. Masanori, Y. Koyama, K. Takakuda, H. Miyairi, N. Shirahama, and J. Tanaka, J biomed mater. Res., 62(2), 265 (2002). https://doi.org/10.1002/jbm.10248
  12. S. D. Nath, N. T. B. Linh, A. Sadiasa, and B. T. Lee, J. Biomater. App., 28(8), 1151 (2014). https://doi.org/10.1177/0885328213499272
  13. N. T. Phuong and B. T. Lee, J. Biomater. App,. 27(3), 311 (2012). https://doi.org/10.1177/0885328211404265
  14. B. Francesco, M. Ferraris, O. Bretcanu, E. Verne, and C. Vitale-Brovarone, J. Biomater App., 0(0), 1 (2011).
  15. V. Jayachandran, and S. K. Kim. Mar. Drugs, 8(8), 2252 (2010). https://doi.org/10.3390/md8082252
  16. N. T. B. Linh, and B. T. Lee, Tissue Engg Part A, (2014).
  17. V. V. Sandra, P. Dubruel, and E. Schacht, Biomacromolecules, 12(5), 1387 (2011). https://doi.org/10.1021/bm200083n
  18. A. T. Neffe, C. Wischke, M. Racheva and A. Lendlein, Expert Rev. Med. Devices, 10(6), 813 (2013). https://doi.org/10.1586/17434440.2013.839209
  19. S. D. Nath, C. Abueva, B. R. Kim, and B. T. Lee, Carbohydr. Polym., (2014).
  20. Y. K. Kolambkar, K. M. Dupont, J. D. Boerckel, N. Huebsch, D. J. Mooney, D. W. Hutmacher and R. E. Guldberg, Biomater., 32(1), 65 (2011). https://doi.org/10.1016/j.biomaterials.2010.08.074
  21. G. Hannink, and J. J. Arts, Injury, 42, S22 (2011).
  22. F. Bai, Z. Jinkang, W. Zhen, L. Jianxi, C. Jiang, L. Jian, M. Guolin, and D. Xin, Biomedical Materials, 6(1), 015007 (2011). https://doi.org/10.1088/1748-6041/6/1/015007
  23. C. M., Murphy, M. G. Haugh, and F. J. O'Brien, Biomater., 31(3), 461 (2010). https://doi.org/10.1016/j.biomaterials.2009.09.063