• Biomolecules & Therapeutics
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• v.26 no.4
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• pp.380-388
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• 2018

Neural stem cells (NSCs) have the ability to self-renew and differentiate into multiple nervous system cell types. During embryonic development, the concentrations of soluble biological molecules have a critical role in controlling cell proliferation, migration, differentiation and apoptosis. In an effort to find optimal culture conditions for the generation of desired cell types in vitro, we used a microfluidic chip-generated growth factor gradient system. In the current study, NSCs in the microfluidic device remained healthy during the entire period of cell culture, and proliferated and differentiated in response to the concentration gradient of growth factors (epithermal growth factor and basic fibroblast growth factor). We also showed that overexpression of ASCL1 in NSCs increased neuronal differentiation depending on the concentration gradient of growth factors generated in the microfluidic gradient chip. The microfluidic system allowed us to study concentration-dependent effects of growth factors within a single device, while a traditional system requires multiple independent cultures using fixed growth factor concentrations. Our study suggests that the microfluidic gradient-generating chip is a powerful tool for determining the optimal culture conditions.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.13 no.1
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• pp.1-4
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• 2003

In the present work we consider morphological structure of the faces of BGO crystals grown by Czochralski technique under the conditions of low temperature gradient (0.1~1 deg/cm) and interconnection between the morphological features of faces at the crystallization front and the formation of defects within the crystal volume. It is demonstrated that the {112} faces retain stability while the growing surface deviates from the crystallographic (112) plane up to 1 degree. At larger deviation, the region of the stable facet growth passes either to the region of macrosteps or to the region of normal growth. depending on conditions.

• Journal of the Korean Ceramic Society
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• v.29 no.3
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• pp.189-194
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• 1992

In order to grow a 127.86$^{\circ}$rotated LiNbO3 single crystal with good characteristics of surface acoustic wave (SAW) up to 80 mm in diameter, the temperature gradient of furnace, the growth rate and the rotation rate of crystal were changed. We could grow a crystal which had few macro defects at the conditions of temperature gradient as 30~6$0^{\circ}C$/cm, growth rate as 5 mm/hr and rotation rate as 8 rpm. The experimental ranges of the growth conditions are as follows. Temperature gradient was varied from 20 to 20$0^{\circ}C$/cm, growth rate as 5~7 mm/hr and crystal rotation rate as 6~12rpm.

• Journal of Korean Society of Forest Science
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• v.71 no.1
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• pp.50-54
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• 1985

The effect of relations parameters on the declination gradient of trees was studied in natural forest of Quercus variabilis, Quercus ${\times}$ grosseserrata, Quercus aliens, and Quercus dentata grown at various slope gradient. The declination gradients of trees were effected by the species, slope gradient, and direction of slope, but not effected by density and height in all species. The species shows a superior factor effecting declination gradient of trees compared with other relations parameters, and next effective factor was slope gradient. Especially in all of slope gradient, the declination gradient of Quercus ${\times}$ grossesserrata was highest.

• Journal of the Korean Ceramic Society
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• v.56 no.6
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• pp.589-595
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• 2019

The top-seeded solution growth (TSSG) method is an effective approach for the growth of high-quality SiC single crystals. In this method, the temperature gradient in the melt is the key factor determining the crystal growth rate and crystal quality. In this study, the effects of the aperture at the top of the hot-zone on the growth of the SiC single crystal obtained using the TSSG method were evaluated using multiphysics simulations. The temperature distribution and C concentration profile in the Si melt were taken into consideration. The simulation results showed that the adjustment of the aperture at the top of the hot-zone and the temperature gradient in the melt could be finely controlled. The surface morphology, crystal quality, and polytype stability of the grown SiC crystals were investigated using optical microscopy, high-resolution X-ray diffraction, and micro-Raman spectroscopy, respectively. The simulation and experimental results suggested that a small temperature gradient at the crystal-melt interface is suitable for growing high-quality SiC single crystals via the TSSG method.

• Korean Journal of Environmental Biology
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• v.27 no.1
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• pp.82-87
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• 2009

In order to characterize the ecological traits of Quercus acutissima, Q. variabilis and Q. mongolica, which dominated in Korean mountain, we treated the sapling of the three oak species under the major environment factors (light, soil moisture and nutrient) with four gradient levels, for 8 months in glass house. Then we measured and analyzed the growth difference among them. The growth of Q. acutissima and Q. variabilis were increased with higher light intensity, but there is no apparent trend in Q. mongolica for light gradients. Q. mongolica did not show high reduction of growth, even in the lowest light intensity. Q. variabilis and Q. mongolica had a constant growth state to soil moisture treatment, but only Q. acutissima grew well in higher soil moisture gradient condition. All the growth of three oak species decreased with higher nutrient gradient condition. The growth reduction was increased in order of Q. variabilis, Q. mongolica and Q. acutissima. with increased nutrient gradient level. These results means that Q. mongolica, Q. acutissima and Q. variabilis have adaptation ability to shade, high moisture and low nutrient condition, respectively.

• Journal of Life Science
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• v.20 no.4
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• pp.597-601
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• 2010

Quercus dentata (Thunb. ex Murray) is a major tree found in dry habitats such as limestone areas of Korea. In order to characterize the ecological traits of Q. dentata, we treated Q. dentata saplings under four gradient levels of major environment factors such as light, soil moisture and nutrients for 5 months in a glass house. We then measured and analyzed growth differences among them. Regarding light, aboveground, belowground and plant biomass were highest at a high gradient and lowest at a low one. The root/shoot ratio was highest at the highest light gradient. Regarding moisture, no measured items were significantly affected by the moisture gradient. Regarding nutrients, aboveground, belowground and plant biomass were the highest at a slightly high gradient and the lowest at a gradient lower or higher than this. The root/shoot ratio was not significantly affected by the nutrient gradient. From these results, it was shown that the growth of Q. dentata was more affected by light and nutrients in the environment than moisture.

• Journal of Environmental Impact Assessment
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• v.18 no.3
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• pp.161-170
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• 2009

As urban growth continues, the earth ecosystem is increasingly dependent on the patterns of urban growth. The impact intensity from urban growth is expected to change predictably with distance from the urban center. However we can't fully understand yet how urban development pattern affects urban ecosystem. In researches about urban ecosystem, it is important to relate the spatial pattern of urbanization to ecological processes. So we used gradient analysis with time data; 1980's, 1990's and 2000's. We attempted to quantify the urban spatiotemporal impacts in Daejeon-city and Cheonan-city, Korea, along a 75km long and 3km wide transect. Through the results, we found the impacts range of urbanization with urban development process of two cities. When the urban growth was concentrated on in both cities, the impacts intensity and range were much stronger and wider. As a result, in urban planning or green space planning, we have to consider suitable urban development forms with surrounding areas, and make legal clauses which limits landuse change. This quantifying the urban gradient is an important step in understanding urban ecology.

• The Korean Journal of Zoology
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• v.35 no.2
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• pp.231-239
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• 1992

For the analysis of the relative grouch of six body pans, such as carapace width. abdominal length, abdominal width, cheliped length, second pereiopod length and third pereiopod length, to the carapace length of crayfish (Comboroides similis Koelbel), the equation Y = bXa were applied. The results were as follows: 1. In the relative srourth of each body part to the carapace length, abdominal width and abdomial length in both sexes showed as positive allometrv, the third pereiopod length in both sexes as isometry and the rest shouted as negative. The least coefficient of the relative growth was found in cheliped length in female (0.9300).2. The grolwth gradient of each body part to the carapace length in each sex revealed decreasing pattern in abdominal length and third pereiopod length in both sexes and the abdominal width in male with the growth centers on the initial growth period. and the rest showed increasing pattern with the growth centers on the 6th growvth period except the abdomini,d uridth in female on the 3rd growth period.

• Environmental Engineering Research
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• v.21 no.4
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• pp.365-372
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• 2016

The effects of hydrodynamics and alum dose on particle growth were investigated by monitoring particle counts in a rapid mixing process. Experiments were performed to measure the particle growth and breakup under various conditions. The rapid mixing scheme consisted of the following operating parameters: Velocity gradient (G) ($200-300s^{-1}$), alum dose (10-50 mg/L) and mixing time (30-180 s). The Poisson regression model was applied to assess the effects of the doses and velocity gradient with mixing time. The mechanism for the growth and breakup of particles was elucidated. An increase in alum dose was found to accelerate the particle count reduction. The particle count at a G value of $200s^{-1}$ decreased more rapidly than those at $300s^{-1}$. The growth and breakup of larger particles were more clearly observed at higher alum doses. Variations of particles due to aggregation and breakup of micro-flocs in rapid mixing step were interactively affected by G, mixing time and alum dose. Micro-flocculation played an important role in a rapid mixing process.