• Clinical and Experimental Reproductive Medicine
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• 제34권1호
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• pp.33-39
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• 2007

목 적: 자궁강 내 인공수정을 위한 과배란유도 시 혈청 vascular endothelial growth factor (VEGF) 농도가 과배란유도의 결과를 반영할 수 있는지를 확인해 보고자 하였다. 연구방법: 과배란유도 후 자궁강 내 인공수정을 시행 받은 49 명의 불임여성을 대상으로 hCG 투여 일에 혈청을 얻어 VEGF-A 및 estradiol 농도를 측정하였다. 과배란유도는 clomiphene citrate (100 mg/d on day 3$\sim$7) 와 human menopausal gonadotropin (150 IU every other day starting on day 5) 병합요법을 이용하였다. hCG 투여 일에 17mm 이상의 성숙난포 수와 자궁내막 두께를 동시에 측정하였다. 결 과: 혈청 VEGF-A 농도는 성숙난포 수, estradiol 농도 및 자궁내막 두께와는 무관하였던 반면 성숙난포 수와 estradiol 농도는 양의 비례관계를 보였다. 혈청 VEGF-A 농도는 성숙난포 수가 2 개 이하인 저 반응 군과 6 개 이상인 고 반응 군에서 통계적으로 유의하지는 않지만 낮은 수치를 보였다. 결 론: 혈청 VEGF-A 농도는 자궁강 내 인공수정 시술 시 과배란유도의 결과와 무관한 것으로 사료되지만 저 반응 군과 고 반응 군에서 낮은 농도를 보이는 것으로 보아 이들을 대상으로 한 추가 연구가 필요할 것으로 판단된다.

• 한국조경학회지
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• 제20권1호
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• pp.53-68
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• 1992

This study was carried out to estimate the recreation carrying capacity of lawn areas in parks. Recreation carrying capacity in this study is composed of two parts, ecological carrying capacity and psychological carrying capacity. As the first part, this paper deals with the treading times criteria based on the tolerence of the turf to treading pressure. The plant material used for this study was Zoysia japonica, and the treading experiment was done in the experimental fields in Kyungju and Suwon, Korea. The major results of the first part of the study are summarized as follow: 1. As shown in annual change of the growth of the turf, The growth of turt grass was represented by the number of leaf. 2. The tolerance to treading was not significantly different by the time of treading treatment. And the tolerance varies depending on growth rate of grasses, which showed significant relationship with precipitations. 3. From the result of different numbers of simulated treading treatment, the damage of the turf was increased by the increase of the frequency of treading. And the damage was very serious within short period(about 20 days) in high frequency(over 20 times/day) of treading treatment. But low frequency(1∼3times/day) of treading shows good effects on the growth of the turf. 4. The criteria of treading number per day for each carrying capacity level are estimated as 7 times/day for optimum carrying capacity, 13 times/day for standard carrying capacity, and 20 times/day for critical carrying capacity.

• 한국물환경학회지
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• 제33권5호
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• pp.538-545
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• 2017

The purpose of this study is to investigate growth response and toxigenicity under various temperature and nutritional conditions, in order to understand the physioecological characteristics of Aphanizomenon flos-aquae, which is a bloom-forming cyanobacterium in the Nakdong River. The strain was inoculated into media under combinations of four temperatures (4, 12, 21, $30^{\circ}C$) and three nutrients (modified CB medium, P-depleted CB medium, N-depleted CB medium) for 28 days. The algae-inhibition tests were performed to assess the potential allelopathic effects of the strains' filtrates on the growth of four algae strains (Microcystis aeruginosa, Aulacoseria ambigua f. spiralis, Aphanizomenon flos-aquae, Scenedesmus obliquus). Toxin production of a strain was measured by Enzyme-Linked ImmunoSolbent Assay (ELISA). The optimal growth temperature (Topt) of strains was $19.9^{\circ}C$ ($18.3-21.2^{\circ}C$), and the temperature range for growth was from $-0.3^{\circ}C$ to $34.3^{\circ}C$. Specific growth rate (${\mu}$) in modified CB medium varied from 0.10 to $0.16day^{-1}$, and the maximum growth rate (${\mu}_{max}$) was $0.17day^{-1}$. Although growth curves under N-existed and N-depleted conditions were almost the same, growth under N-depleted condition was relatively slowed (${\mu}=0.09$ to $0.14day^{-1}$), with a decreased maximum cell density. However, growth under the P-depleted condition was restricted for all temperatures, Two stains of Aphanizomenon flos-aquae were confirmed as not producing toxins, because saxitoxin and cylindrospermopsin were not detected by ELISA. The exudates or filtrates from the Aphanizomenon flos-aquae (DGUC003) resulted in significant inhibition of algal growth on the Aulacoseira ambigua f. spiralis (DGUD001) and Aphanizomenon flos-aquae (DGUC001) (p < 0.01).

• 부모자녀건강학회지
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• 제10권1호
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• pp.13-24
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• 2007

Purpose: To investigate the health status such as growth, nutrition, disease, vaccination in child day care centers. Methods: A cross-sectional descriptive study was conducted on 280 children at 6 day care centers in Seoul. Parent completed a structured questionnaire and illness-logs from day-care staff were gathered for a month, June 2006. Body weight, height were measured and BMI was calculated. Results: Failure of growth was found in 2.2% of the children. Also there was 5.4% of the children who were under 5% and 8.6% were above 95% on BMI chart. For 4.3% of the children diseases were asthma, chronic rhinitis, sinusitis and so on. About six percent of the children missed vaccination such as Japanese encephalitis, DTaP and polio. Incidence rates of respiratory infection was 1.45 and gastroenteritis was .28. As a result, 22.5 % of the day care children had these kinds of health rick factors stated above and results showed statistical difference between health-groups and health-risk groups in age, duration of day care center, family structure, marital status of parent. Conclusion: A considerable number of the children at child daycare centers had the health risk factors. These results shows there is a need to develop and make provisions for health service programs by health experts in child day care centers.

• 대한핵의학회지
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• 제1권1호
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• pp.37-54
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• 1967

The changes in histopathology of various organs and growth inhibition of the chick embryos incubated with radioactive sulfur ($^{35}S$) were experimentally studied. The various doses of $^{35}S$ were injected into the yolk sac at different intervals and the weight changes of the embryos were evaluated to determine the growth inhibition rates. The embryos sacrified on various incubation days were used for the study of histopathological changes in organs such as the bone, liver, kidney, gonad, and eye. Following were the results: 1) The weight changes of the $^{35}S$ treated groups were as follows: i. Embryos treated on the 5 th incubation day: No weight changes were noted on the 8th incubation day, however, the growth inhibition rate of 32.1% was noted in the group treated with $50{\mu}C$ and of 38.2% in the group treated with $150{\mu}C$ on the 12th incubation day. The rates were 9.1 and 12.1% on the 15th incubation day, and 6.5 and 10.6% on the 18th incubation day respectively. ii. Embryos treated on the 8th incubation day: The growth inhibition rates on the 12th, 15th and 18th incubation days in the groups treated with $50{\mu}C$ were 20.9, 25.9 and 18.8% and in those treated with $150{\mu}C$ were 20.0, 14.9 and 16.9% respectively. iii. Embryos treated on the 12th incubation day: The growth inhibition rates on the 15th and 18th in the groups treated with $50{\mu}C$ were 13.6 and 21.1% and in those treated with $150{\mu}C$ were 26.7 and 6.5% and in those treated with $250{\mu}C$ were 10.6 and 12.6% respectively. iv. Embryos treated on the 15th incubation day: The growth inhibition rates on the 18th in the groups treated with $50{\mu}C$ were 6.5% and in those treated with $150{\mu}C$ were 10.1% and in those treated with $250{\mu}C$ were 8.5% respectively. In summary, the longer the incubation days, the less the growth inhibition rates. II) The histopathological changes in the various organs were as follows: i. Bone: Hyperplasia and edematous changes of the bone cavity, irregular distribution of immature granular cells and increased number of the myeloblast, megakaryocyte and reticuloendothelial cells were noted. ii. Liver: The embryos treated with $150{\mu}C\;of\;^{35}S$ on the 8th incubation day showed necrosis and nucleolysis of the liver cell and abnormal enlargement of sinusoid on the 12th incubation day. The longer the incubation days, the more severe the changes such as the pyknotic artophy of the liver cells and heterochromatism. The embryos treated on the 5th incubation day with 50 and $150{\mu}C\;of\;^{35}S$ showed little changes, but sight enlargement and accumulation of serous fluid in the sinusoid on the 8th incubation day. iii. Kidney: No particular changes except atrophic changes of epithelium were noted in early stage, however, the infiltration of the granular cell and monocyte into the cortex and pyknotic changes of vascular glomeruli were noted in later stage. These changes were not closely related to the doses of $^{35}S$ given. iv. Gonad: The degenerative changes such as destruction of the immature germ cells, hyperplasia and vacuolization of the stroma were noted in testis and ovary. v. Eye: A slight distortion of the cornea and sclera was noted. The hypertrophy of inner layer and blood cell infiltration into the vascular layer of the choroid membrane were noted in embryo groups on the 12, 15 and 18th incubation days.

• Radiation Oncology Journal
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• 제12권3호
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• pp.301-305
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• 1994

Purpose :. To investigate the effect of aqueous extract of Ganoderma lucidum(G.I.) on the surival of tumor cells in vitro and on the growth of tumors in vivo. Materials and Methods : Dried G.I. was made into powder, extracted with distilled water, filtered and diluted from a maximum concentration of 100 mg/ml in sequence. The cytotoxicity of G.I, in vitro was evaluated from its ability to reduce the clonogenicity of SCK tumor cells. For the tumor growth delay study, about $2{\times}10^5$$ of SCK tumor cells were subcutaneously inoculated in the legs of A/J mice. The first experimental group of mice were injected i.p. with 0.2ml of 250 mg/kg of G.I. from the first day after tomor inoculation for 10 days. The second experimental group of mice were injected i.p. with 0.2ml of 250 mg/kg of G.I. either once a day for 10 days or twice a day for 5 days beginining from the 7th day after tumor inoculation Results : 1. Cytotoxicity in vitro;survival fraction, as judged from the curve, at G.I. concentration of 0.5, 1,5, 10, 25, 50 and 100 mg/ml were 1.0, $0.74{\pm}0.03$, $0.18{\pm}0.03$, $0.15{\pm}0.02$, $0.006{\pm}0.002$, 0.015 and 0.0015, respectively. 2. Tumor growth delay in vivo; a) the time required for the mean tumor volume to grow to $1,000mm^3$ was 11 days in the control group and 14 days in the experimental group. b) the time required for tumor volume to increase 4 times was 11 days in the control group while it was 10.5 and 12 days in the groups injected with G.I. once a day and twice a day from the 7th day after tumor inoculation respectively. Conclusion : Aqueous extracts of G.I. showed a marked cytotoxicity on the SCK mammary cells in vitro. Tumor growth delay was statistically signiricant when G.I. in-jection was started soon after tumor inoculation, but it was not significant when injection was started after the tumors were firmly established.

• 대한치과보철학회지
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• 제32권2호
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• pp.268-280
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• 1994

Tissue conditioners have been used for treatment of denture stomatitis caused by wearing of dentures. Early studies pointed out Candide albicans (C. albicans) as main etiologic factor, and antifugal agents were added for control of the species. But there is a little information about broad comparison on the effect of tissue conditioners and antifungal agents added. The purpose of the present study was to compare the inhibiting effect of four tissue conditioners and one temporary soft liner on the growth of C. albicans for treatment of denture stomatitis using gel diffusion method by measuring diameter of the zone of growth inhibition. Three antifungal agents were added to each material for evaluation of the effect of added agents. Finally, observation was made to evaluate the effect of the loss of antifungal elements by aging of the specimen. The results of this study were obtained as follows : 1. Tempo had remarkable antifungal effect showing the zone of growth inhibition as 2.35 mm at 1st day, and was most effective on End: 4th and 7th day from incubation (p<0.05). But Coecomfort, Dura conditioner, Visco-gel, Coe-soft had little antifungal effect from the 1st day of incubation. 2. Nystatin was most effective showing 9.60-12.04 mm of zone of inhibition at the 1st day from incubation. The antifungal properties were reduced to amphotericin B, chlorhexidine and materials without agent (p<0.05), and the effect was diminished by time. 3. As pretreatment with amphotericin B, nystatin, chlorhexidine, Tempo was very effective at the 1st day from incubation showing zone of inhibition as 3.65, 12.04, 4.78 mm with addition of each agent. Dura conditioner had strongest antifungal effect at the next day as 2.86, 5.33, 1.29 mm of zone of inhibition, and yielding results of Coe-comfort, Tempo, Coe-soft was shown at 4th and 7th day from incubation (p<0.05). Taken all together, tissue conditioners have little antifungal effect except Tempo. Formation of the zone of growth inhibition was due to agents amphotericin B, nystatin, chlorhexidine and nystatin was most effective. Conclusively, it is advisable to select material which is effective on the growth of C. albican and consider addition of antifungal agents for treatment of denture stomatitis.

• Weed & Turfgrass Science
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• 제4권3호
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• pp.256-261
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• 2015

답압스트레스는 골프장 등에 한국잔디(Zoysia japonica)로 조성된 잔디밭에서 인간의 이동, 농기계나 차량의 이동 등에서 종종 발생한다. 본 연구는 잔디의 생육에 답압 스트레스가 미치는 영향을 알아보기 위하여 무답압(0 J $day^{-1}$), 약답압(1,570 J $day^{-1}$), 중답압(4,710 J $day^{-1}$), 강답압(9,420 J $day^{-1}$) 4수준의 답압조건에서 지상부와 지하부 길이, 광합성 능력, 엽수, 건물중, 2-DE 단백질 분석을 실시하였다. 지상부 길이는 답압을 주게 되면 답압을 하지 않은 무처리구 비해 급격하게 줄어들었고, 답압의 충격량이 증가할수록 길이가 짧아지는 경향을 나타냈다. 지하부 길이는 답압을 한 처리구가 답압을 하지 않은 무처리구 보다 길이가 길었고, 그 중 약답압을 한 처리구에서 가장 길었다. 광합성 능력은 답압을 주게 되면 답압의 충격량이 증가할수록 감소하는 경향을 나타내었다. 엽수는 답압을 하지 않은 무처리구에서 가장 많이 나왔고, 답압의 충격량이 증가할수록 감소하는 경향을 나타내었다. 지상부 건물중은 답압이 증가할수록 감소하는 것으로 확인 되었고, 중답압과 강답압에서는 큰 차이를 보이지 않았다. 한국잔디에 답압을 주게 되면 그에 따른 스트레스에 적응하기 위해 반응하는 단백질이 발현되었다. 단백질 변성을 막아주는 heat shock 70 kDa protein 10와 광합성에 관련된 Oxygen-evolving enhancer protein 1이 발현이 증가 되었다. 이상 종합해보면 답압 조건에 따른 한국잔디의 생육에 차이가 있었으며, 일정수준의 답압이 잔디 생육에 있어서 지상부 생육은 답압하지 않은 것보다 저조 하였으나 지하부 생육은 다소 촉진되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제12권3호
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• pp.388-394
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• 1999

Two experiments were conducted to subdivide isoleucine (exp. 1) and valine (exp. 2) requirements for maintenance from the requirements for growth of broilers aged 1 to 3 weeks. Purified diets were used, containing five graded levels of isoleucine and valine. Based on weight gain response, the isoleucine requirement for growth was 7.50 mg/g weight gain and the daily isoleucine need for maintenance (mg) was 0.044 per unit metabolic body size ($(Wg^{0.75})$). Based on the N gain response, the isoleucine requirement for growth was 0.317 mg/mg N gain and the daily isoleucine need for maintenance (mg) was 0.040 per unit metabolic body size $(Wg^{0.75})$. Based on weight gain and N gain response, the total isoleucine requirement was calculated 244 mg/day or 0.59% of the diet, 274 mg/day or 0.66% of the diet, respectively. From the relationship of weight gain and N gain, 5.07% of the retained protein was comprised of isoleucine; the reported isoleucine content of chick muscle was 4.42%. The valine requirement for growth was 9.84 mg/g weight gain and 0.36 mg/mg N gain whereas the maintenance requirement was 0.046 or 0.052 mg per unit of metabolic body size (Wgo.11. According to the model developed to estimate valine requirement, the total requirement was 319 mg/day or 0.77% of the diet, 315 mg/day or 0.76% of the diet, respectively. Previous reported valine requirements for growing chicks of 7~24 days old were in close agreement with these estimates. As a percentage of retained protein, valine was calculated to be 5.81% ; the reported valine concentration of crude protein of chicks' body including feathers was 6.72%.

• 한국수산과학회지
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• 제22권5호
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• pp.332-341
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• 1989

경상남도 일광 연안으로 흘러내리는 하천의 하구 기수역에서 미끈날망둑온 채집하여 이석과 위내용물을 분석하여, 자치어기의 나이, 성장율 및 식성을 추정하였다. 이석 미세성장선의 하루주기성은 약 15일 간격으로 연속하여 채집된 어류 이석을 각 채집일을 감안하여 성장선 배열의 공통성을 비교 분석하여 증명하였다. 체장의 성장은 Gompertz의 식으로 유의하게 표시되었다. $L=5.73{\cdot}\;e^{15.06}(1-e^{-0.0015t})$관찰 범위에서 체장의 일일성장율은 부화 후 $50\~60$일 사이에는 0.40 mm/day로 작았으나 자라면서 빨라져 $90\~100$일 사이에는 0.85 mm/day로 증가하였다. 위내용물 분석 결과, 미끈날망둑은 전형적인 육식성 어종으로 밝혀졌는데, 주된 먹이생물은 polychaetes, amphipods, copepods 였으며 이들은 위내용물의 대부분을 차지하였다. 미끈날망둑은 성장에 따라 다소 먹이 선택에 있어 변화를 보이는데, $15\~30$mm 크기군에서는 copepods가 비교적 많이 섭이 되었지만, 성장함에 따라 amphipods의 섭이가 증가되었다. 한편 polychaetes의 선호도는 모든 크기군에 걸쳐 가장 높았다.