• Journal of fish pathology
• /
• v.35 no.1
• /
• pp.103-111
• /
• 2022

This study has been performed to investigate the potential effects of by-product discarded after probiotics production (BPPP) on growth performance, immune gene expression, innate-immunity status, and disease resistance of carp, Cyprinus carpio. For 3 weeks, carp were fed four diets containing different levels of BPPP at 0, 0.1, 0.2 and 0.5% per kg of normal diet. Every 7 days of feeding, immune-related gene expression, serum lysozyme activity and ACH₅₀ were analyzed. Growth rates and challenge test with E. tarda were conducted after 3 weeks of BPPP feeding. Both lysozyme activity and ACH₅₀ were significantly (p<0.05) increased in all BPPP supplemented groups compared to the control at every 7 day for 3 weeks of feeding trial. The gene expression of pro-inflammatory cytokines, IL-1β and TNF-α was significantly (p<0.05) up-regulated until 21 days of feeding in all groups except for 0.2% group on day 7 post feeding. The anti-inflammatory cytokine IL-10 gene expression was only significantly (p<0.05) increased in 0.1% group on day 7 and decreased (p<0.05) on day 14 in all BPPP supplemented groups. On day 21, the IL-10 gene expression was augmented (p<0.05) in all groups. SOD gene expression was significantly (p<0.05) increased compared to the control on day 14 and 21 post feeding, whereas no significant difference was observed on day 7. In challenging test, 0.2%, 0.1%, 0.5% and control group showed 80%, 70%, 60% and 40% of survival rate, respectively. Feed conversion rate was only improved in 0.5% group. In conclusion, the present study indicates that dietary BPPP suplementation improved growth performance, innate immune response and bactericidal activity in carp.

• Asian Journal of Turfgrass Science
• /
• v.15 no.2
• /
• pp.65-76
• /
• 2001

This study was designed to estimate on the interaction of the first productivity, light condition and to examine the ecophysiological characteristics of Zoysia japonica. rtificial community of Zoysia japonica was analyzed effect of matter production and growth construction under various shading condition. The results summarized this experiment were as follows; 1. The relative growth rate (RGR) in all experimental plots was high during the growth stage of 20 days after transplanting, and then decreased. The maxium RGR value appeared in the control plot (2.13g/g. 10days) during the growth stage from 10 to 20 days after transplating. RGR value in server shading of short day condition was remarkably decreased from the early growth stage. The main factor to lower RGR value considered as a short day condition than that of shading. 2. The net assimilation rate (NAR) in all experimental plots except 3hour photoperiod plot was high during the early growth stage after transplanting, and then decreased early growth stage after transplanting, and then decreased as the growth proceeded. The maxium value appeared in the control plot (35g/g. 10days). NAR value in the severe shading of short day condition plots was low. Especially, NAR value in the three hours photoperiod plot was remarkably low from the early growth, there was no great difference by growth stage. NAR had negative correlation with LAI and positive correlation with RGR. 3. The increasing rate of leaf area ratio(LAR) was high during the early growth stage after transplanting in the control plot and in the photoperiod plot, the shorter the exposure time to sunlight, the higher the LAR value from the early growth stage. Especially, its value in the 3 hour photoperiod plot was remarkably high but its increasing rate was lower as the growth proceeded. LAR hade negative correlation on RGR and NAR. 4. The crop growth rate(CGR) in each experimental plot was increased until 50 days after transplanting, and then decreased. After that time, the maxium CGR value appeared in the control plot (1.56g/$\textrm{cm}^2$. 10-days), 60 days after transplanting, CGR had positive correlation with LAI. The optimum LAI in the control, 9 hour, 7 hour, 5 hour, and 3 hour photoperiod plots appeared as 1.87, 1.12, 0.83, and 0.18.

• The korean journal of orthodontics
• /
• v.22 no.2 s.37
• /
• pp.309-325
• /
• 1992

The purpose of this study was to evaluate the effect of intrinsic factor and extrinsic factor for growth of the mandibular condylar cartilage of 4 day-old rats in a serum-free medium for 1, 4, 7, 14 days. They were compared with normal growth in vivo and with growth of spheno-occipital synchondrosis in serum-free medium. The cellular kinetics of cartilages were evaluated by auto-radiography of tritiated thymidine. 1. Condylar cartilage was enlarged with rounded head on day 14 of experiment while in vivo the rounded-headed shape changed into functionally flattened appearance. 2. On day 14 of experiment, a severe reduction of the proliferative zone and a considerable increase of the hypertrophic zone were observed while in normal control group endochondrol bone formation and bone marrow were observed. 3. The proliferative activity in the proliferative zone of condylar cartilage detected by $^3H-thymidine$ incorporation was lower than that of normal control group and decreased more than that of spheno-occipital synchondrosis, but it continued during the 14 days of culture. 4. The continued maintenance of condylar cartilage and morphologic change were disturbed in this culture system, but cell division within the proliferative zone was continued and probably linked to intrinsic factor.

• KSBB Journal
• /
• v.25 no.2
• /
• pp.193-198
• /
• 2010

Cell cultures of Taxus wallichiana Zucc. were made to enhance the production of anticancer agent paclitaxel. In suspension cultures, the maximum cell growth rate in exponential growth phase was 0.14 $day^{-1}$ which was correlated to 4.96 days of cell doubling time. The production of paclitaxel was non-growth associated. The paclitaxel production was started after the exponential growth phase and increased to declined phase where the maximum concentration was observed. Various elicitors were tested to enhance the production of paclitaxel. The combination of two elicitors of jasmonic acid and cellulase increased the production of paclitaxel 1.8 and 3.1 times compared to paclitaxel production by individual elicitor respectively.

• Mycobiology
• /
• v.37 no.4
• /
• pp.277-285
• /
• 2009

In this study, dual culture, poison agar, and direct methods were used to assess the ability of Trichoderma virens IMI-392430, T. pseudokoningii IMI-392431, T. harzianum IMI-392432, T. harzianum IMI-392433, and T. harzianum IMI-392434 to control Ceratocystis paradoxa, which causes the pineapple disease of sugarcane. The highest percentage inhibition of radial growth (PIRG) values were observed with T. harzianum IMI-392432 using two dual culture methods, 63.80% in Method I and 80.82% in Method II. The minimum colony overgrowth time was observed with T. harzianum IMI-392432 and the maximum was observed with T. pseudokoningii IMI-392431. Different concentrations of different day-old metabolites of Trichoderma isolates were tested against mycelial growth of C. paradoxa. The highest PIRG (84.685%) exhibited at 80% concentration of 30-day-old metabolites of T. harzianum IMI-392432 using the modified bilayer poison agar method. In the direct assay method the maximum mycelial growth weight (PIGW) was observed at the same concentration and the same day-old metabolites of T. harzianum IMI-392432. This study showed that Trichoderma isolates have a good antagonistic effect on C. paradoxa mycelial growth and T. harzianum IMI-392432 has the most potential to control the pineapple disease pathogen.

• Korean Journal of Agricultural Science
• /
• v.48 no.2
• /
• pp.201-207
• /
• 2021

This study evaluated the influence of enzyme mixture supplementation on the growth performance, nutrient digestibility, and fecal score of growing pigs. A total of 72 pigs with an initial body weight of 20.23 ± 1.46 kg were randomly assigned to two treatments consisting of a basal diet and the basal diet supplemented with 0.5% enzyme mixture. During a 19-day trial, no significant difference was observed in the body weight (BW) and average daily feed intake (ADFI) of the pigs. However, a gradual increase in the average daily gain (ADG) was observed during the period from day 14 to day 19 and the overall period in pigs fed a diet supplemented with the 0.5% enzyme mixture (p < 0.10) as compared to the pigs that were fed the control diet. From days 4 to 14 and in the overall experiment, a gradual increase in the feed conversion ratio (FCR) (p < 0.10) was observed with the inclusion of 0.5% enzyme mixture supplementation. The nutrient digestibility of dry matter (DM), nitrogen (N), and energy were not affected by enzyme mixture supplementation. In addition, dietary supplementation with the enzyme mixture had no significant effects on the fecal score of growing pigs. In summary, supplementation with the enzyme mixture had beneficial effects on the ADG performance but failed to have a significant effect on growth performance (BW), nutrient digestibility, and fecal score.

• Animal Bioscience
• /
• v.34 no.10
• /
• pp.1653-1662
• /
• 2021

Objective: This experiment was conducted to investigate the effect of insoluble cellulose supplementation to diets on the growth performance, intestinal morphology, the incidence of diarrhea, nutrients digestibility, and inflammatory responses in altering environmental conditions of animals housing. Methods: A total of 108 male pigs (Duroc×[Yorkshire×Landrace]) were randomly allocated to one of three dietary treatments (cellulose 0%, 1%, 2%) and two environmental conditions (good sanitary condition vs. poor sanitary condition) to give 6 replicate pens per treatment with three pigs per each pen at 14 days post-weaning. Results: Pigs were in good sanitary condition had higher average daily gain (p<0.01) and improved feed efficiency (p<0.05) from day 1 to 14 after weaning compared to their counterparts. The interactions were found between environmental conditions and dietary treatments (day 7: crypt depth [p<0.01], villous height to crypt depth [p<0.001]; day 14: crypt depth [p<0.001], villous to crypt ratio [p<0.01]) in ileum morphology. Crypt depth was decreased (p<0.05), and villous to crypt ratio was increased (p<0.05) only in poor sanitary conditions. Pigs exposed to the good sanitary condition had higher (p<0.05) apparent ileal digestibility (day 7, gross energy; day 14, dry matter), apparent total tract digestibility (day 14, dry matter and crude protein) compared to pigs housed in the poor sanitary condition. Meanwhile, pigs fed a diet supplemented with 2% cellulose had decreased (p<0.05) apparent ileal digestibility (day 7, dry matter; day 14, crude protein), apparent total tract digestibility (day 7, dry matter; day 14, crude protein, gross energy) compared to pigs fed a diet supplemented with 0% or 1% cellulose. Conclusion: Our results indicated that a diet supplemented with 1% cellulose increased villous to crypt ratio, however feeding a diet containing cellulose (1% or 2%) impaired nutrient digestibility for 14 day after weaning in both good sanitary and poor sanitary conditions.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.51 no.1
• /
• pp.64-71
• /
• 2018

This study was conducted to investigate the effects of light intensity and wavelength on the growth of Tetraselmis suecica and Tetraselmis tetrathele. These species were exposed to a blue light-emitting diode (LED; max=450 nm), a yellow LED (max=590 nm), a red LED (max=630 nm) and a fluorescent lamp (three wavelengths). The maximum growth rates (${\mu}_{max}$) of T. suecica and T. tetrathele under a red LED were 1.12/day and 0.95/day, respectively. Under a yellow LED, growth rates were 70% of the values for red wavelength, with low half-saturation constants (Ks). The optimum light source to ensure economically effective and productive growth in a Tetraselmis culture system (Photo-Bioreactor) would thus appear to be a three-phase culture, wherein a yellow LED is used during the lag phase and initial exponential phase to increase growth rate, followed by a red LED during the middle exponential phase to maximize growth rate, and finally a yellow LED again during the late exponential phase and stationary phase to achieve increased yield of useful bioactive substances.

• Journal of Nutrition and Health
• /
• v.29 no.10
• /
• pp.1142-1149
• /
• 1996

This study was conducted to determine changes in th contents and composition of dietary fiber during the growth of soybean sprout. Soybean was soaked in water at $25^{\circ}C$ for 2hrs and cultivated at 2$0^{\circ}C$ for 7 days under dark condition. The soybean sprouts were divided into cotyledon and axis and sampled every 24hrs. The analysis methodlogies used were Van Soest's NDF, AOAC's ADF and lignin and Prosky's IDF, SDF, TDF. The weight of 100 sprouts increased gradually from 20.26g to 90.12g during the growth periods. The weight increased to 344.9% of the original weight. The germination rate was 100% after soaking at $25^{\circ}C$ for 2hrs. Root length increased gradualy from 0.6cm at 1st day to 17.2cm at 7th day. The crude ash and crude fat contents showed no significant change in the cotyledon and axis. The crude protein contents increased in the cotyledon and axis, whereas the total carbohydrate content didn't have general tendency. The insoluble dietary fiber(IDF), soluble dietary fiber(SDF) and total dietary fiber(TDF) contents of cotyledon were no significantly different from 20.01%, 1.45%, 21.46% at 1st day to 22.75%, 2.07%, 24.82% at 7th day on dry basis. In axis those contents increased from 23.19%, 1.97%, 25.16% at 1st day to 32.78%, 3.02%, 35.80% at 7th day, respectively. The neutral detergent fiber(NDF) contents of cotyledon and axis increased from 4.35% to 6.39% and from 6.44% to 26.60% respectively on dry basis. The acid detergent fiber (ADF) contents of cotyledon and axis increased from 2.84% to 4.91% and from 2.5% to 4.7%, but there were no significantly different in the hemicellulose and lignin contents on dry basis. The hemicellulose and lignin contents of axis increased with culture periods from 1.70% to 4.41% and from 0.20% to 2.11%, respectively. The cellulose contents increased from 4.54% to 20.35% on dry basis.

• KSBB Journal
• /
• v.10 no.4
• /
• pp.401-405
• /
• 1995

An open pond type photobioreactor for mass cultivation of S. platensis was designed and the growth parameters from different cultivation processes were compared. 0.30(1/day) of specific growth rate and 1.69(g/$\ell$) of maximum cell density were obtained from batch cultivation. In fed-batch cultivation, specific growth rate and maximum cell density were estimated as 0.22(1/day) and 1.75(g/$\ell$), respectively. Maximum biomass productively from continuous cultivation was obtained as 0.44 (g/$\ell$/day). It proves that an outdoors-mass cultivation of S. platensis considering optimal environmental condition is economically feasible. In addition, the biomass productivity was studied in two different mixing systems such as agitation and air sparging methods. The biomass productivity by an agitation method was better than that in an air sparging method.