• Maxillofacial Plastic and Reconstructive Surgery
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• v.28 no.5
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• pp.375-395
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• 2006

Purpose: Lingual nerve (LN) damage may be caused by either tumor resection or injury such as wisdom tooth extraction, Although autologous nerve graft is sometimes used to repair the damaged nerve, it has the disadvantage of necessity of another operation for nerve harvesting. Moreover, the results of nerve grafting is not satisfactory. The nerve growth factor (NGF) is well-known to play a critical role in peripheral nerve regeneration and its local delivery to the injured nerve has been continuously tried to enhance nerve regeneration. However, its application has limitations like repeated administration due to short half life of 30 minutes and an in vivo delivery model must allow for direct and local delivery. The aim of this study was to construct a well-functioning $rhNGF-{\beta}$ adenovirus for the ultimate development of improved method to promote peripheral nerve regeneration with enhanced and extended secretion of hNGF from the injured nerve by injecting $rhNGF-{\beta}$ gene directly into crush-injured LN in rat model. Materials and Methods: $hNGF-{\beta}$ gene was prepared from fetal brain cDNA library and cloned into E1/E3 deleted adenoviral vector which contains green fluorescence protein (GFP) gene as a reporter. After large scale production and purification of $rhNGF-{\beta}$ adenovirus, transfection efficiency and its expression at various cells (primary cultured Schwann cells, HEK293 cells, Schwann cell lines, NIH3T3 and CRH cells) were evaluated by fluorescent microscopy, RT-PCR, ELISA, immunocytochemistry. Furthermore, the function of rhNGF-beta, which was secreted from various cells infected with $rhNGF-{\beta}$ adenovirus, was evaluated using neuritogenesis of PC-12 cells. For in vivo evaluation of efficacy of $rhNGF-{\beta}$ adenovirus, the LNs of 8-week old rats were exposed and crush-injured with a small hemostat for 10 seconds. After the injury, $rhNGF-{\beta}$ adenovirus($2{\mu}l,\;1.5{\times}10^{11}pfu$) or saline was administered into the crushed site in the experimental (n=24) and the control group (n=24), respectively. Sham operation of another group of rats (n=9) was performed without administration of either saline or adenovirus. The taste recovery and the change of fungiform papilla were studied at 1, 2, 3 and 4 weeks. Each of the 6 animals was tested with different solutions (0.1M NaCl, 0.1M sucrose, 0.01M QHCl, or 0.01M HCl) by two-bottle test paradigm and the number of papilla was counted using SEM picture of tongue dorsum. LN was explored at the same interval as taste study and evaluated electro-physiologically (peak voltage and nerve conduction velocity) and histomorphometrically (axon count, myelin thickness). Results: The recombinant adenovirus vector carrying $rhNGF-{\beta}$ was constructed and confirmed by restriction endonuclease analysis and DNA sequence analysis. GFP expression was observed in 90% of $rhNGF-{\beta}$ adenovirus infected cells compared with uninfected cells. Total mRNA isolated from $rhNGF-{\beta}$ adenovirus infected cells showed strong RT-PCR band, however uninfected or LacZ recombinant adenovirus infected cells did not. NGF quantification by ELISA showed a maximal release of $18865.4{\pm}310.9pg/ml$ NGF at the 4th day and stably continued till 14 days by $rhNGF-{\beta}$ adenovirus infected Schwann cells. PC-12 cells exposed to media with $rhNGF-{\beta}$ adenovirus infected Schwann cell revealed at the same level of neurite-extension as the commercial NGF did. $rhNGF-{\beta}$ adenovirus injected experimental groups in comparison to the control group exhibited different taste preference ratio. Salty, sweet and sour taste preference ratio were significantly different after 2 weeks from the beginning of the experiment, which were similar to the sham group, but not to the control group.

• Clinical and Experimental Pediatrics
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• v.48 no.8
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• pp.846-856
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• 2005

Purpose : To measure the peak myocardial tissue velocities and patterns of longitudinal motion of atrioventricular(AV) annuli and assess body weight and heart rates-related changes in normal children. Methods : Using pulsed wave Tissue Doppler Imaging(TDI), we measured peak systolic, early and late diastolic myocardial velocities in 72 normal children at six different sites in apical-4 chamber (A4C) view and at four different sites in apical-2 chamber(A2C) view and compared those values with each other, also observing effects with body weights and heart rates. Longitudinal motions of the AV annuli were measured at three different sites in A4C. Results : There were no significant differences of the TDI parameters between gender, ECHO-machines and among the three Doctors performing TDI. Peak myocardial velocities were significantly higher at the base of the heart than in the mid-ventricular region and in the right lateral ventricular wall than in the left lateral ventricular wall or IVS. The TDI parameters showed no significant correlation with fractional shortening(%). Peak systolic and early diastolic myocardial velocities had no correlation with heart rates, but peak late diastolic velocities and A/E ratio correlated positively with heart rates. Correlations between the TDI parameters and body weight were inconsistent. Absolute longitudinal displacement and % displacement were not differ between gender and not correlated with the TDI parameters. Conclusion : We measured the peak myocardial velocities with TDI and the longitudinal motion of the AV annuli using M-mode echocardiography in normal children. With more large scale evaluation, we may establish reference values in normal children and broaden clinical applicabilities in congenital and acquired heart diseases.

• Geophysics and Geophysical Exploration
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• v.11 no.1
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• pp.34-40
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• 2008

Underwater seismic refraction with advanced interpretation approaches makes important contributions to shallow marine exploration and geotechnical investigations in Australia's coastal areas. A series of case studies are presented to demonstrate the recent applications of continuous and static USR methods to river crossing and port infrastructure projects at various sites around Australia. In Sydney, static underwater seismic refraction (USR) with bottom-placed receivers and borehole seismic imaging assisted the development of improved geotechnical models that reduced construction risk for a tunnel crossing of the Lane Cove River. In Melbourne, combining conventional boomer reflection and continuous USR with near-bottom sources and receivers improved the definition of a buried, variably weathered basalt flow and assisted dredging assessment for navigation channel upgrades at Geelong Ports. Sand quality assessment with continuous USR and widely spaced borehole information assisted commercial decisions on available sand resources for the reclamation phase of development at the Port of Brisbane. Buried reefs and indurated layers occur in Australian coastal sediments with the characteristics of laterally limited, high velocity, cap layers within lower velocity materials. If these features are not recognised then significant error in depth determination to deeper refractors can occur. Application of advanced refraction inversion using wavefront eikonal tomography to continuous USR data obtained along the route of a proposed offshore pipeline near Fremantle allowed these layers and the underlying bedrock refractor to be accurately imaged. Static USR and the same interpretation approach was used to image the drowned granitic regolith beneath sediments and indurated layers in the northern area of Western Australia at a proposed new berthing site where deep piling was required. This allowed preferred piling sites to be identified, reducing overall pile lengths. USR can be expected to find increased application to shallow marine exploration and geotechnical investigations in Australia's coastal areas as economic growth continues and improved interpretation methods are developed.

• The Journal of the Petrological Society of Korea
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• v.22 no.3
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• pp.219-233
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• 2013

Spherulitic rhyolite occur as part of ring dyke which showing a vertical flowage of $60^{\circ}{\sim}90^{\circ}$, of the Jangsan cauldron was studied. The spherulites range in diameter from a few millimeters to 2.8 centimeters or more, and average 5~10 millimeters. It belongs to radiated simple spherulite type. They consist of a core of moderate brown dense material encased by a thin crust, a few millimeters thick at most of white grey material. The spherulites frequently have a radiating fibrous structure, which are thought to have formed as a consequence of rapid mineral growth caused by very fast cooling of the dykes in shallow depth near the surface. EPMA examination of the concentric-zoned core of spherulites show that they are mainly composed of cryptocrystalline-fibrous intergrowth of silica minerals and alkali feldspars which have $SiO_2$ 82% or more, $Al_2O_3$ 7~10%, $Na_2O+K_2O$ less than 8%. The feldspar compositions of the spherulites lie essentially within the sanidine field. XRD examination show that spherulites are mainly composed of quartz, sanidine, albite with minor mica, kaolinite and chlorite. According to X-ray mapping, the spherulites are enriched in $SiO_2$ in the core and partly enriched $Na_2O$ or $K_2O$, $Al_2O_3$ in the shell that reflect in compositional zoning with increasing spherulitic devitrification. The feathery and non-equant crystal shapes of spherulites from rhyolite dyke of Jangsan cauldron suggest that they may have formed during the rapid cooling of dyke under the static state, or faster velocity of devitrification from glassy materials than movement velocity of the magma intrusion. The spherulitic rhyolite originated from high-silica(75.4~75.7 wt.%) rhyolite magma.

• Journal of the Korean Society of Marine Environment & Safety
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• v.18 no.6
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• pp.610-616
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• 2012

All the countries in the world is currently facing the full scale of energy-climate era currently, and making strong energy policy that will lead to green growth of the future energy resources by utilizing renewable energy as the basis of entering the advanced country becomes the goal of development that satisfies the demand for energy in 21st century. Recently, ocean energy attracted the attention along with the necessity of developing renewable energy. Ocean energy is the one of most prominent recyclable and clean resources that has not been developed yet. So, it is highly required to develop good tidal current energy conversion system in coastal area. The inflow angle that acts against tidal current turbine, seabed effect and the change of efficiency along the occurrence of cavitation were investigated through the wake flow characteristics in this study. Power coefficient degradation by seabed effect did not appear in the condition of this calculation. Efficiency degradation appeared from above $10^{\circ}$ regarding inflow angle and power coefficient was calculated as lower by 7 % at $45^{\circ}$. Torque and power coefficient increased as inflow velocity rose, but power coefficient degradation appeared from above 3m/s when the cavitation happened. So, it was recognized that the larger inflow angle and occurrence of cavitation become the reason for power degradation through the flow characteristics.

• Journal of Conservation Science
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• v.32 no.3
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• pp.353-364
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• 2016

The materials of sculptured stone property around the Hyeonleung Royal Tomb in Guri consist mainly of high weathered of granitic rocks with magnetite-series. Deterioration characteristics occurred highly with microorganism, soil inflow and black contaminants at the burial mound zone. As a result of deterioration evaluation, stone surface around the burial mound zone show serious comprehensive damage of soil inflow (50.5%) and lichen coverage (47.6%) which are the major damaging factors, and there are about 8.6% of biological growth noticeably in the north side. Surface contaminants and the discoloration had the complex causes. Gypsum occurred between joints of stones and the major contaminant type, darkening which was analyzed organic bodies caused discoloration. From result of ultrasonic velocity measurements, there are mean value of 2,195 m/s with highly weathered (HW) grade. Most of the stone properties showed 4 to 5 weathered grade. Therefore, it turned out that sculpture stone properties require conservation treatments. To remove soil accelerating damage factors and lichen occupying high percents, the cleaning process is necessary and consolidation, rejoining and filling are needed as well. Also, consideration on removing conservation materials containing Ca and e fflorescence is required and retreatments need to be considered.

• Microbiology and Biotechnology Letters
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• v.31 no.2
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• pp.171-176
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• 2003

Productivity of biosurfactant (rhamnolipid) by Pseudomonas aeuginosa F722 was investigated in the several culture conditions and culture composition. Biosurfactant production by P. aeuginosa F722 was amounted to 0.78 g/l as the result of the nitrogen sources and carbon sources without investing of optimum conditions. As for that one was investigated, biosurfactant production by P. aeruginosa F722 was amounted to 1.66 g/l. Biosurfactant production increased twofold because the composition of a modified C-medium was investigated efficiently. $NE_4$Cl or $NaNO_2$ inorganic nitrogens and yeast extract or trypton organic nitrogens were effective, but others inorganic nitrogens and organic nitrogens tested were not efficient far biosurfactant production by P. aeruginosa F722. The optimum concentration of $NH_4$Cl; inorganic nitrogen and yeast extract; organic nitrogen were 0.05% and 0.1%, respectively. In various carbon sources, others with the exception of hydrophobic property substrate (n-alkane) and hydrophilic property substrate (glucose, glycol) were not found to be effective fur biosurfactant production, and 3.0% was better in yield than other concentration of glucose. This yielded C-to-N ratios between 17 and 20. In our experiment, the highest biosurfactant production by P. aeruginosa F722 were observed in 5 days cultivation, containing glucose 3.0%, $NH_4$Cl 0.05%, and yeast extract 0.1% and C-to-N ratio was 20. Optimal pH and temperature for biosurfactant production were 7.0 and $35^{\circ}C$, respectively. Under the optimal culture conditions with glucose, biosurfactant production was amounted to 1.66 g/l. Velocity of biosurfactant production and strain growth increased after nitrogen depletion. The average surface tension of 30 mN/m after the 3 days of incubation under optimal culture condition was measured by ring tensionmeter.

• Cartoon and Animation Studies
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• s.40
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• pp.109-137
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• 2015

As Digital technology developed, drawing animation, using traditional production method which expresses many feelings with drawn lines loses the youth group, the primary audience, to the realism of the digital cinema. Drawing animation, which drew attention of the youth by the spectacles in the film such as hectic pace, dynamics and punch, declined for a while. However, it has been developed a way to express pace and dynamics of its own by establishing an effective directing method, which combines digital technology as it is needed. This study has a purpose to investigate what causes the dynamics and feeling of fast movement of the character in Japanese limited animation. Though some action-animated films that heroes with supernatural powers take the leading role that feeling of velocity and dynamics are emphasized we compare the directing method before and after the introduction of the digital technology. This research reaches the conclusion by factoring each Bergson and McLuhan's discussion to the intervention of indexical signs and the audience's participation according to skipping technique. This study has a significance of researching the element of drawing animation that maximizes the expansion of the senses by defying the limitation of the law of physics through its unique way of directing together with growth of the hero films, which will continue.

• Korean Journal of Ichthyology
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• v.17 no.3
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• pp.195-204
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• 2005

The population ecology of Rhinogobius brunneus was investigated on, tributaries of the Bukhan River from May 2003 to June 2005. The habitat was mostly a rapids area with pebbles and cobles in the lower part of the stream. The cohabitation fishes were Zacco platypus, Zacco temmincki, Microphysogobio yaluensis and Iksookimia koreensis. The males and females become sexually mature after one year. Spawning season was from May to June with water temperatures in the range $22{\sim}25^{\circ}C$. The prosperous period was from late May to early June. The average number of eggs in the ovary was $989{\pm}511$ (range, 151~2,209). The year-round sex ratio of female to male was 1 : 0.90. The total length in this population indicated that the below-40 mm group is one year old, 40~52 mm group is two years old, 52~65 mm group is three years old, and the longer-than-65 mm group is over four years old. There were no differences in total length distribution between the males and females. Nests were built up at a water depth of $13.7{\pm}5.8$ (5~31) cm and a current velocity of $9.6{\pm}4.8$ (10~19) cm/sec. Brood size (egg number) of the parental male was $1,974{\pm}1810$ (egg number = $95.022_{TL}-3507$, $r^2=0.3591$, N = 33). Newly hatched larvae that drifted downstream to the lake returned to the stream during late June to August; the smallest size in this population was 20 mm in total length.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.20 no.5
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• pp.391-407
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• 1987

To understand the effects of the cooling water system of the power plant on the succession of sessile zoobenthos, a series of experiments were carried out at the cooling water system of Samchonpo Power Plant from Hay 1985 to May 1986. The lowest species diversity of the zoobenthos was observed at the discharge canal. This is probably caused by both increased velocity and temperature of circulating water. It is also noticeable that species composition of the zoobenthos, to a certain degree, was affected due to the impingement of meroplankton during the entrainment process. While the number of species attached on the experimental substrata installed in the thermally altered area is smaller than that in the intake canal, the biomass is increased as the increased temperature eliminates some species and in turn stimulates reproduction and growth of the others. It is interesting that relatively high similarity exists between the summer communities in the intake canal and the spring communities in the thermally altered area, and the same relationship is also found between the fall communities in the intake canal and the winter communities in the thermally altered area. Ecological index curves of the zoobenthos of the intake canal follows the fluctuation pattern of the thermally altered area in one or two months of elapsed time, probably due to temperature increase by the inflow of heated water discharged from the power plant.