• 생명과학회지
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• 제18권7호
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• pp.986-991
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• 2008

본 연구는 남자초등학교 4학년생 중 태권도 유품자($1{\sim}2$품)로서 태권도칼슘섭취군(8명), 태권도군(8명), 대조군(8명) 총 24명을 대상으로 하였다. 태권도와 칼슘 섭취가 남자초등학생의 체격, 성장호르몬 및 IGF-1에 미치는 영향을 규명하기 위하여 운동 강도 RPE ($7{\sim}15$), 운동빈도 주 5회, 1회 50분의 지속시간으로 12주간 실시하였다. 칼슘은 1일 1회 저녁에 750 mg을 섭취시킨 결과 다음과 같은 결론을 얻었다. 신장은 집단 내 세 그룹 모두 유의하게 증가하였지만 집단 간에서는 대조군에 비해 태권도군과 태권도칼슘섭취군이 유의하게 증가하였다. 성장호르몬은 대조군이 유의하게 감소하였으며 태권도군과 태권도칼슘섭취군은 대조군에 비해 유의하게 증가하였다. IGF-1은 집단 내 태권도군과 태권도칼슘섭취군이 유의하게 증가하였으며 집단 간에서는 대조군에 비해 태권도군과 태권도칼슘섭취군이 유의하게 증가하였다. 이상의 결과를 통해 초등학생에게 태권도프로그램과 칼슘섭취는 신장과 체중, 성장호르몬 및 IGF-1에 긍정적인 영향을 미쳤으며, 향후 적절한 태권도프로그램개발과 운동처방 및 영양섭취에 대한 지속적인 연구가 요구된다.

• Asian-Australasian Journal of Animal Sciences
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• 제21권12호
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• pp.1760-1765
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• 2008

A total of 60 one-day-old Yellow-feather broiler chickens were allotted into treatment and control groups. The treatment group was fed with the diet supplemented with 3% conjugated linoleic acid (CLA) for 48 d, while control group was fed with the diet supplemented with 3% rapeseed oil. Chickens were slaughtered in each group at the age of 49 d, and the blood and the abdominal adipose tissue were sampled. Serum cLeptin and serum cAdiponectin were measured by ELISA. The total RNA was extracted from adipose tissue to measure the abundance of the chicken growth hormone receptor (cGHR), insulin-like growth factor 1 (cIGF-1), insulin-like growth factor I receptor (cIGF-IR), peroxisome proliferator-activated receptor gamma ($cPPAR{\gamma}$), cAdiponectin and cAdipoIR mRNA by RT-PCR using ${\beta}$-actin as an internal standard. Results showed that the CLA decreased the abdominal fat index by 20.93% (p<0.05). The level of serum cLeptin but not serum cAdiponectin was significantly increased by CLA treatment (p<0.05). CLA down-regulated the relative abundance of cGH-R mRNA and $cPPAR{\gamma}$ mRNA in abdominal adipose tissue by 24.74% (p<0.05) and 66.52% (p<0.01) respectively. However, no differences were found between CLA treatment group and control group (p>0.05) in the relative abundance of cIGF-1, cIGF-IR, cAdiponectin, and cAdipoIR mRNA in abdominal adipose tissue. The data suggested that CLA inhibited abdominal fat deposition in broiler chicken may be determined by decreasing the GHR available for GH, and by inhibiting the differentiation of preadipocytes via down-regulation of $PPAR{\gamma}$, but independent of IGF and (or) GH-IGF pathway or adiponectin action.

• Fisheries and Aquatic Sciences
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• 제20권8호
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• pp.17.1-17.8
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• 2017

Somatostatin (SS) and growth hormone-releasing hormone (GHRH) are primary factors regulating growth hormone (GH) secretion in the pituitary. To date, it remains unknown how this rhythm is controlled endogenously, although there must be coordination of circadian manners. Melatonin was the main regulator in biological rhythms, and its secretion has fluctuation by photic information. But relationship between melatonin and growth-related genes (ghrh and ss) is unclear. We investigated circadian rhythms of melatonin secretion, ghrh and ss expressions, and correlation between melatonin with growth-related genes in tiger puffer Takifugu rubripes. The melatonin secretion showed nocturnal rhythms under light and dark (LD) conditions. In constant light (LL) condition, melatonin secretion has similar patterns with LD conditions. ss1 mRNA was high during scotophase under LD conditions. But ss1 rhythms disappeared in LL conditions. Ghrh appeared opposite expression compared with melatonin levels or ss1 expression under LD and LL. In the results of the melatonin injection, ghrh and ss1 showed no significant expression compared with control groups. These results suggested that melatonin and growth-related genes have daily or circadian rhythms in the tiger puffer. Further, we need to know mechanisms of each ss and ghrh gene regulation.

• 대한한방소아과학회지
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• 제35권2호
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• pp.1-10
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• 2021

Objectives We aimed to examine health-related economic analysis of available interventions on idiopathic short stature (ISS). Methods Eight studies were reviewed from English, Korean, and Chinese databases which were published up to December 24, 2020. Effectiveness, utility, and cost data were extracted from the studies and descriptive analysis of the individual studies was conducted Results Five studies were chosen. In the two economic evaluation studies, the incremental cost-effectiveness ratio (ICER) of growth hormone (GH) treatment was presented by performing cost-effectiveness analysis based on the deterministic decision tree approach for the GH and untreated group. Final adult height and direct medical costs were analyzed as effectiveness and cost outcomes. In 1 review article, an ICER of GH was presented based on systematic review on the effects of the GH treatment. In the two clinical trials, the effectiveness and cost of the 12 months Oriental medicine combination treatment were presented in comparison with the GH treatment alone. There were no literatures that provided utility data of available intervention on ISS. Conclusions The results of this study will be used as basic data for the economic analysis of Oriental medicine treatment on ISS in the future.

• Archives of Plastic Surgery
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• 제35권1호
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• pp.13-19
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• 2008

Purpose: Human adipose tissue-derived mesenchymal stem cells(hATSCs) can be differentiated into multiple mesenchymal lineages, including bone, cartilage, and muscle. And growth hormone play important roles in the normal growth and development of the CNS. In this study, we explored whether the transplanted hATSCs and growth hormones could improve functional recoveries from rats with contusive spinal cord injury. Methods: We divided 30 female rats, which were subjected to a weight driven implant spinal cord injury, into 3 groups with 10 rats each; Group A as a control group, group B with hATSCs transplantation on injured region, and group C with hATSCs transplantation and GH administration for 7 days. Then, we researched their neurologic functional recoveries before and 2, 4, and 8 weeks after transplantation using Basso-Beattie-Bresnahan (BBB) locomotor rating scale. And we checked Y-chromosome positive cells by FISH(Fluorescent in situ hybridization) to identify the survival of transplanted mesenchymal stem cells. Results: After 4 weeks of transplantation, the group B and group C showed significant improvement of neurologic function on BBB locomotor rating scale in comparison with the group A(Group A: $13.1{\pm}0.58$, Group B: $14.6{\pm}0.69$, Group C: $14.9{\pm}0.56$). Moreover, the group C displayed meaningful recovery of neurologic function after 8 weeks in comparison with group B (Group B: $15.7{\pm}0.63$, Group C: $16.5{\pm}1.14$). The group A, the control one, improved for 5 weeks after injury, and had no more recovery. On the other hand, Group B and C showed the improvement of neurologic function continuously for 9 weeks after injury. Conclusion: In this study, we found out that hATSCs transplantation have an effect on neurologic functional recovery of spinal cord injured rat and GH injection seems to bring the synergistic results on this good tendency.

• 생명과학회지
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• 제19권11호
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• pp.1644-1650
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• 2009

본 연구는 운동을 하지 않는 여자 중학생을 대상으로 걷기운동이 체구성, 체력, 심혈관 기능, 혈청지질, GH, IGF-1 및 Leptin에 미치는 효과를 분석하기 위하여 걷기운동을 12주간 수행한 결과 비만집단과 일반집단 모두 체구성, 체력 심혈관 기능, 혈청지질 및 인슐린 인자에서 긍정적인 변화를 보였으며, 특히 비만집단은 체지방의 감소효과가 컸고, 일반집단은 근육량의 향상에 효과가 컸으며, 두 집단 모두 체력요인의 향상이 있었고 심혈관 기능과 혈청지질 및 인슐린 인자의 변화에도 여러 요인에서 유의한 효과가 있었다. 이러한 결과는 청소년시기에 바쁜 생활패턴에서 걷기운동만으로도 여중생의 체구성과 체력을 정상적으로 유지시킬 수 있는 좋은 운동 프로그램이 될 수 있음을 나타내고 있다. 그러나 이러한 긍정적인 변화를 지속시키기 위해서는 올바른 식습관(diet)과 보다 긴 걷기운동시간이 필요하다. 본 실험의 대상자들 또한 실험기간 중 정해진 운동시간외에 개인적 걷기운동량이 하루 15분정도 증가하였다. 운동추후의 연구에서는 운동기간의 증가와 운동 강도의 증가에 따른 지속적인 변화의 분석을 위한 연구가 필요하다고 생각된다.

• 생명과학회지
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• 제27권9호
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• pp.1047-1051
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• 2017

노화 과정에 따라 중년 여성들은 폐경 이후 체력이 저하되기 시작하면서 체지방 증가와 제지방량의 감소로 비만으로 이어지기 쉽고, 이러한 현상은 체내 호르몬의 분비의 변화와 함께 발생된다. 본 연구는 서킷 트레이닝이 비만 중년 여성의 노화 관련 호르몬에 미치는 영향을 구명하기 위해 실시하였다. 대상자 체지방률 30% 이상인 비만 중년 여성 운동군 10명, 대조군 10명 총 20명을 실시하였다. 운동 시간은 준비운동 5분, 본 운동 20분, 정리운동 5분으로 총 30분 주 3회 실시하였으며, 운동 강도는 운동자각도를 이용하여 13~17(약간 힘들다-매우 힘들다)수준으로 실시하였고, 또한 POLAR 심박수 변화량을 측정하여 1~6주는 60~70%HRR (Heart rate reserve), 7~12주는 70~80%HRR로 설정하였다. IGF-1은 운동군이 유의하게 증가하였으며, GH는 운동군이 유의하게 증가하였고, DHEA-S는 운동군이 유의하게 증가하였다. 이상의 결과 서킷 트레이닝이 비만 중년 여성의 노화 관련 호르몬에 긍정적인 영향을 준 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제17권3호
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• pp.343-348
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• 2004

The objective of the present study was to determine the plasma level of insulin-like growth factor-I (IGF-I) in relation to mammary blood flow and milk yield including biological variables of relevance to milk synthesis in two different types of crossbred Holstein cattle at 3 different stages of lactation. Eight heifers were 87.5% HF and eight 50% HF animals were selected for the experiments. The three stages of lactation tested were: early lactation (30 days postpartum), mid-lactation (120 days postpartum) and late lactation (210 days postpartum). Animals in each group were fed a concentrate and rice straw treated with 5% urea as the source of roughage throughout the experiments. In early lactation, mammary blood flow and milk yield of 87.5% HF animals were significantly higher than those of 50% HF animals. In mid- and late lactation, both mammary blood flow and milk yield showed a proportional decrease from the early lactating period of 87.5% HF animals. The trends for persistency were observed in 50% HF animals as for udder blood flow and milk yield throughout the experimental periods. The plasma glucose level of the 50% HF animals was significantly higher than those of 87.5% HF animals in both early and mid-lactation. The concentrations of arterial plasma free fatty acids ($C_{16}\;to\;C_{18}$) were higher in 50% HF animals as compared with 87.5% HF animals in all periods of study. In early lactation, the concentration of plasma growth hormone (GH) of 87.5% HF animals was higher than those of the 50% HF animals, thereafter the mean level of plasma growth hormone declined in both mid- and late lactation. The concentration of plasma IGF-I of 50% HF animals was significantly higher than those of 87.5% HF animals in all stages of lactation. There were no differences among stages of lactation for the levels of plasma IGF-I, insulin and growth hormone in 50% HF animals. In 87.5% HF animals, the plasma levels of both IGF-I and insulin were lower in early lactating period while it showed an increase during mid- and late lactation. The present results indicated that the regulatory role for the higher mammary blood flow and milk yield during lactation in 87.5% HF are not mediated via the higher level of circulating IGF-I. Differences in mammary blood flow and milk yield between 50% HF and 87.5% HF animals are in part due to a higher concentration of circulating growth hormone. The lower level of circulating growth hormone in 50% HF animals would be regulated by higher levels of IGF-I, free fatty acid and glucose in plasma.

• Archives of Pharmacal Research
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• 제26권11호
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• pp.929-936
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• 2003

Methanol extract (MeOH), n-hexane (Hx), chloroform ($CHCl_3$), ethyl acetate (EA), butanol (BuOH) and aqueous ($H_2O$) fractions of Eucommiae Cortex including geniposidic acid (GA), geniposide (GP) and aucubin (AU) were tested for their therapeutic efficacy on osteoporosis. The contents of GA, GP and AU in the cortex and leaf of Eucommia ulmoides Oliver were quantified by HPLC. The effect of Eucommiae Cortex on the induction of growth hormone (GH) release was studied by using rat pituitary cells. The proliferation of osteoblast-like cells increased by herbal extracts was assayed using a tetrazolium (MTT), alkaline phosphatase (ALP) activity, and [$^3H$]-proline incorporation assays. The inhibition of osteoclast was studied by using the coculture of mouse bone marrow cells and ST-2 cells. As a result, the GA, GP and AU were present in the cortex more than in the leaf of E. ulmoides Oliver. The MeOH (1mg/mL), Hx, $CHCl_3$ and EA fractions (each 20 $\mu$ g/mL) had potent induction of GH release. The $CHCl_3$ exhibited the potent proliferation of osteoblasts. The AU, GP and GA were increased proliferation of osteoblasts. In addition, GA ($IC_{50}: 4.43{\times}10^{-7}$M), AU and GP were significantly inhibited proliferation of osteoclast. In summary, it is thought that the components in a part of the fractions of Eucommiae Cortex participate in each step of mechanism for activating osteoblast to facilitate osteogenesis, and suppress osteoclast activity to inhibit osteolysis.

• Journal of mucopolysaccharidosis and rare diseases
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• 제1권2호
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• pp.44-48
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• 2015

Body fat distribution in patients with Prader-Willi syndrome (PWS) is characterized by reduce lean body mass (LBM), increased total body fat mass (FM), and lower percentage of visceral adipose tissue (VAT). Individuals with PWS seem to have a lower risk for insulin resistance with high levels of adiponectin, an anti-atherogenic adipocytokine that is decreased in visceral fat hypertrophy subjects compared to simple obese subjects, both in children and in adults. The mechanism of the reduction in visceral adiposity in PWS is still unclear. It might be related to qualitative intrinsic characteristics of adipocyte or novel genetic influences on the control of fat distribution. However, obesity remains a critical problem, and obesity status plays a crucial role in individual metabolic risk clustering and development of metabolic syndrome (Mets) in PWS children and adults. Long-term growth hormone (GH) treatment after cessation of skeletal growth improved body composition, with an increase in lean body mass and a reduction in total body fat and subcutaneous and visceral fat in PWS adults. Thus, the role of GH is important after childhood because it might attenuate obesity and Mets in PWS adult by adipocyte modification.