• Title/Summary/Keyword: Growing factor

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Nutritional and Hormonal Induction of Fatty Liver Syndrome and Effects of Dietary Lipotropic Factors in Egg-type Male Chicks

  • Choi, Y.I.;Ahn, H.J.;Lee, B.K.;Oh, S.T.;An, B.K.;Kang, C.W.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.8
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    • pp.1145-1152
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    • 2012
  • This experiment was conducted with male chicks to investigate the influence of hormones and nutrients on the development of fatty liver syndrome (FLS) as well as the effects of dietary lipotropic factors on hepatic fat accumulation and lipogenic enzyme gene expression. A total of two-hundred sixteen 4-wk-old Hy-Line male chicks were divided into six groups and fed an experimental diet (T1, low-energy diet with low levels of lipotropic factors; T2, high-energy diet with low levels of lipotropic factors; T3 and T5, low-energy diet with high levels of lipotropic factors; T4 and T6, high-energy diet with high levels of lipotropic factors) for six weeks. The chicks in T5 and T6 groups were treated with intramuscular injections of estradiol benzoate for three days prior to biopsy and clinical analysis of FLS. Chicks treated with estrogen had significantly greater liver weights than untreated chicks. The abdominal fat contents were increased in chicks consuming high-energy diets as compared to those consuming low-energy diets. Treatment with estrogen significantly increased the concentrations of serum cholesterol, triacylglycerol and phospholipid (p<0.05). The hepatic triacylglycerol levels were tenfold higher in the estrogen treated chicks than in the untreated chicks. There were no significant differences in malondialdehyde levels between the treatment groups. Estrogen treatment dramatically increased the levels of fatty acid synthetase, acetyl-CoA carboxylase and ApoB mRNA. The results indicated that treatment with exogenous estrogen in growing male chicks induced hepatic fat accumulation, which might be partially due to increased lipogenic enzyme gene expression.

증발억제법에 의한 수온 및 지온상승효과에 관한 연구

  • 김광식
    • Water for future
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    • v.5 no.2
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    • pp.6-16
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    • 1972
  • It has been well studied and known that the yields from the rice fields irrigated by the cold water such as the water directly flowing in from mountain valleies, underground water and subground water are largely influenced by the water temperature. However, the best method of raising water temperature has not yet been established. This is because there are some essentially difficult problems associated. When we examine the effects of $1^{\circ}C$ rise in the water temperature under natural condition on rice growing, the necessity of this line of study is verified. The results of Mihara's study show that rice bears its fruits at the water temperature above $19^{\circ}C$ and the difference of $1^{\circ}C$ in the range of $19^{\circ}C$ to $22^{\circ}C$ can produce the 20% of difference in yields. Because of these facts, most farmers have made use of water temperature raising ponds, zigzag waterways and shelter belts. But the most important factor in raising water temperature has been found to be the heat loss due to evaporation. Recently, a good deal of experiment on raising water temperature and soil temperature by reducing the evaporation are being carried out all over the world. The reduction of evaporation does not only reduce heat loss, from the surface but also reduce the loss of water. Present study is aimed to determine the efficiency of different chemicals by which monomolecular films are formed over different surfaces such as water surface, soil surface and the surface of plant leaves with a purpose of preventing the transpiration, and aimed to observe the effects of the temperature rise and its influence on growing state as well as the durability of the plants under drought condition.

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Genotype and Environment Effects on Gliadin Content and Polyphenol Oxidase Activity in Wheat

  • Seo, Yong-Weon;Park, Yong-Hack;Hong, Byung-Hee;Park, Moon-Woong;Nam, Jung-Hyun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.1
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    • pp.38-43
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    • 2000
  • The environment in which a given genotype is grown may influence its grain quality characteristics. When varieties are $\times$ evaluated over numerous environments, a variety environment interaction usually is observed, but the relative magnitude of environmental(E), genetic(G), and G $\times$ E effects on quality is unclear. In order to determine relative contribution of genotype, environment, and G $\times$ E interaction to the variations observed in grain quality characteristics, 18 Korean wheat cultivars and experimental lines were evaluated in two environments in 1998 and 1999. Correlation coefficients between grain quality and agronomic characteristics were also estimated. The analysis of variance for the optical density obtained by reaction bet- ween gliadin and anti-gliadin polyclonal antibody (AGPab) indicated that gliadin content measured by Enzyme-Linked Immunosorbent Assay(ELISA) was significantly in- fluenced by environment and cultivar differences. The significant differences of year and year $\times$ location were also found. The ratio of the variances associated with environmental effects to the variances associated with genetic effect gave relatively greater influence of environmental factor on gliadin content. The different protein content from same genotype grown in different environment might be associated with degree of storage protein accumulations. Significant relationships between ELISA and protein content, yield, ten spike weight, and ten spike number were detected. Polyphenol oxidase (PPO) activity was significantly influenced by year, location, cultivar and year $\times$ location. The variance in grain PPO activities among growing years appeared larger than the variation produced by the cultivar examined. This suggested that the growing environment contributed more to variability in grain PPO concentration.

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Expression of mRNAs and Proteins of Cyclin A and LATS Genes in Ovary (Cyclin A와 LATS 유전자들의 난소 내 mRNA 및 단백질 발현에 관한 연구)

  • Park, Chang-Eun;Kim, Dae-Jung;Hong, Sung-No
    • Korean Journal of Clinical Laboratory Science
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    • v.40 no.1
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    • pp.31-40
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    • 2008
  • Despite of the importance of the primordial follicle (PMF) recruitment, factors and mechanisms for process are poorly understood. To evaluate expression and role of the follicular transition from PMF to PMF/primary follicles (PMIF) in the present study, we evaluated expression of lats1, lats2, cyclin A1, and cyclin A2 mRNA and protein, and elucidated and role of lats1-cyclin A in the follicular transition from PMF to PRIF. To analysis of differential expression in PMF and PMIF, each stage follicles were collected by day1 and day5 of immuno-compromised rats (ICR) and analyzed by real-time PCR for the genes. For localization of mRNAs and proteins of the genes, in situ hybridization and immunohistochemistry were performed. We confirmed that the lats1, lats2, cyclin A1, and cyclin A2 mRNA were more expressed in PMF than PMIF. Localization of the four genes expression were observed in nuclei of oocytes from the arrested primordial, and in the surrounding granulosa cells of the growing follicles. The mRNA expressions were gradually decreased with follicular development. From immunohistochemistry studies, Cyclin A1 protein expression were observed in oocyte cytoplasmas of early stage follicles, while observed in granulose cells and oocyte nucleoli during growing follicles. This study suggested that the presence of lats gene family might perform negatively regulation of cell proliferation by modulation of the CDC2/Cyclin A complex activity. lats-cyclin A genes in oocytes of the early stage follicles might play a role in the meiotic cell cycle arrest of the primary oocytes at the primordial follicle stage as well as the follicular growth.

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Function of rax2p in the Polarized Growth of Fission Yeast

  • Choi, Eunsuk;Lee, Kyunghee;Song, Kiwon
    • Molecules and Cells
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    • v.22 no.2
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    • pp.146-153
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    • 2006
  • Cell polarity is critical for the division, differentiation, migration, and signaling of eukaryotic cells. RAX2 of budding yeast encodes a membrane protein localized at the cell cortex that helps maintain the polarity of the bipolar pattern. Here, we designate SPAC6f6.06c as $rax2^+$ of Schizosaccharomyces pombe, based on its sequence homology with RAX2, and examine its function in cell polarity. S. pombe $rax2^+$ is not essential, but ${\Delta}rax2$ cells are slightly smaller and grow slower than wild type cells. During vegetative growth or arrest at G1 by mutation of cdc10, deletion of $rax2^+$ increases the number of cells failing old end growth just after division. In addition, this failure of old end growth is dramatically increased in ${\Delta}tea1{\Delta}rax2$, pointing to genetic interaction of $rax2^+$ with $tea1^+$. ${\Delta}rax2$ cells contain normal actin and microtubule cytoskeletons, but lack actin cables, and the polarity factor for3p is not properly localized at the growing tip. In ${\Delta}rax2$ cells, and endogenous rax2p is localized at the cell cortex of growing cell tips in an actin- and microtubule-dependent manner. However, ${\Delta}rax2$ cells show no defects in cell polarity during shmoo formation and conjugation. Taken together, these observations suggest that rax2p controls the cell polarity of fission yeast during vegetative growth by regulating for3p localization.

Mechanical Properties of Bamboo-reinforced Boards Manufactured with Phyllostachys bambusoides Growing in Damyang District (I) -- Physical Properties of Bamboo Strand Board -- (담양산 왕대를 이용한 대나무 강화 복합보드의 제조 및 역학적 특성 평가 (I) - 대나무 스트랜드 보드의 물리적 특성 -)

  • 소원택;박병대
    • Journal of Korea Foresty Energy
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    • v.22 no.2
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    • pp.26-35
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    • 2003
  • Bamboo strand board (BSB) was made with Phyllostachys banbusoides growing in Damyang district. Physical and mechanical properties of this BSB were summarized as follows; The specific gravity of BSB was 0.63∼0.79. Specific gravity decreased slightly with the thickness and length of BSB. Moisture content of BSB manufactured was 5.8∼6.9%. The absorption ate of BSB (42∼48%) did not show any relationship with the thickness and length of BSB. The thickness swelling rate of BSB was 13.9∼17.0%, relatively higher than any other panel products. Thickness swelling rate increased with the thickness of BSB, showing the strand thickness influenced much more on the rate of thickness swelling of BSB than the length of strand. The 3-point bending strength of BSB was 98∼126kgf/$\textrm{cm}^2$. Bending strength of showed the tendency of increase with the increased length of BSB, but with the decreased thickness. In particular, the length of BSB showed more effect on the increase of bending strength of BSB than the thickness of BSB. The compression strength perpendicular to BSB surface was 411 ∼ 465 kgf/$\textrm{cm}^2$, and the optimal length of strand for the 1mm- and 2mm-thickness of strand was 40mm and 60mm, respectively. Compression strength paralleled to BSD was 160∼221kgf/$\textrm{cm}^2$ and the optimal length of strand for the 2mm-thickness of strand appeared to be 60mm. The present work showed that appearance, physical and mechanical strength of BSB appeared quite positive in terms of board qualities, suggesting that bamboo would be appropriate for the production of board materials. In addition, our work showed that the crucial factor for determining the mechanical characteristics of BSB was the dimension of strand.

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A Study on Route Decision for Multimodal Transportation - From Viewpoint of Service Factors (복합운송경로 선정에 관한 연구-서비스요인 중심으로)

  • Kim, So-Yeon;Choi, Hyung-Rim;Kim, Hyun-Soo;Park, Nam-Kyu;Park, Yong-Sung;Jung, Jae-Un
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • v.1
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    • pp.251-259
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    • 2006
  • The increase in international logistics market and various customer demands emphasize the importance of Multimodal Transportation, and that market is continuously keep growing. In order to ensure competitive superiority in a market of such infinite competition, service that can satisfy each individual customer by considering various customer characteristics, has become an issue. Thus, through the aspect of service, in order to improve customer satisfaction, growing factors of Multimodal Transportation Route on was studied in this research. For this research, first of all main service factors that affect the growth of Multimodal Transportation were seized by literature survey and positive research. The, by using these factors a methodology that enables individual customers to assess Multimodal Transportation Route was studied. Through this research, individual customers can acquire objective assessment data and Multimodal Transportation companies can seize what factors are considered as important by their customers.

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Effect of Coverings on the Growth of Ginseng and the Persistency of Procymidone in Growing Soils (인삼재배를 위한 차광조건이 인삼의 생육과 토양 중 Procymidone의 잔류에 미치는 영향)

  • Kim, Hyo-Kun;Lee, Kyu-Seung
    • Korean Journal of Environmental Agriculture
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    • v.21 no.1
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    • pp.24-30
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    • 2002
  • Ginseng (Panax ginseng C. A. Meyer) has been cultivated on raised beds with shading condition for 4 to 6 years because of its physiological characteristics. This shading condition maintains relatively stable soil environments protecting from rainfall and direct sunlight. Therefore, pesticides in ginseng growing soil are exposed to far different environment from the general cropping field. To study the behavior of pesticides under this condition, the effect of covering on the persistency of Procymidone in ginseng cultivating soil was investigated by using several shade materials. The most important factor affecting Procymidone persistency in soil under covering was water leakage rates of shade materials.

Underlying Etiologic Factor of Recurrent Pneumothorax after Bullectomy (원발성 기흉환자에서 재수술의 원인)

  • 윤용한;이두연;김해균;홍윤주
    • Journal of Chest Surgery
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    • v.32 no.6
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    • pp.556-560
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    • 1999
  • Background: The cause of spontaneous pneumothorax is not yet but it is certain that intrathoracic air comes from ruptured bulla. Video-assisted thoracoscopic surgery(VATS) or open thoracotomy is recommended for thoracic incision in recurrent pneumothorax. However, recurrent rate after bullectomy with the VATS is very high compared to mini-thoracotomy, 3% to 20% and below 2%, respectively. Material and Method: This retrospective analysis was performed on 16 re-operated cases among 446 surgically treated pneumothorax of the 737 cases of spontaneous pneumothorax diagnosed at Yongdong Severance Hospital from Nov. 1992 to June 1997. Result: Among the 446 surgically-treated patients in 737 case of spontaneous pneumothorax, 16 patients underwent re-operation, showing a 3.5% re-operation rate. Male-to-female ratio was 15 to 1 and mean age at initial attack was 20.2 years(ranging from 15 to 50). Mean hospital stay was 6.34 days(ranging from 2 to 20 days) and mean chest tube indwelling period was 4.2 days(ranging from 1-10 days). Median follow-up was 46 months(range 10-66 months). Three different surgical methods were applied : video-assisted thoracoscopic surgery(VAST) in 281 cases, of whom 2 underwent local anesthesia; subaxillary mini-thoracotomy in 159 cases and limited lateral thoracotomy in the remaining 6 cases. Three different re-operative surgical methods were applied ; video-assisted thoracoscopic surgery (VAST) in 6 cases, subaxillary mini-thoracotomy in 9 cases, and limited lateral thoracotomy in the remaining 1 case. The underlying etiological factors of the recurrent pneumothorax after bullectomy were o erlooking type(9) and new growing type(7). Mean recurrent period from previous operation was 1 month for overlooking type and 18 months for new growing type. Conclusion: The underlying etiological factors of recurrent pneumothorax lead to re-operation were new-growing and over-looking type. We need additional treatments besides resecting blebs of prevent the recurrence rate and more gentle handling with forceps due to less damage to the pleura.

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Definition of Tumor Volume Based on 18F-Fludeoxyglucose Positron Emission Tomography in Radiation Therapy for Liver Metastases: An Relational Analysis Study between Image Parameters and Image Segmentation Methods (간 전이 암 환자의 18F-FDG PET 기반 종양 영역 정의: 영상 인자와 자동 영상 분할 기법 간의 관계분석)

  • Kim, Heejin;Park, Seungwoo;Jung, Haijo;Kim, Mi-Sook;Yoo, Hyung Jun;Ji, Young Hoon;Yi, Chul-Young;Kim, Kum Bae
    • Progress in Medical Physics
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    • v.24 no.2
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    • pp.99-107
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    • 2013
  • The surgical resection was occurred mainly in liver metastasis before the development of radiation therapy techniques. Recently, Radiation therapy is increased gradually due to the development of radiation dose delivery techniques. 18F-FDG PET image showed better sensitivity and specificity in liver metastasis detection. This image modality is important in the radiation treatment with planning CT for tumor delineation. In this study, we applied automatic image segmentation methods on PET image of liver metastasis and examined the impact of image factors on these methods. We selected the patients who were received the radiation therapy and 18F-FDG PET/CT in Korea Cancer Center Hospital from 2009 to 2012. Then, three kinds of image segmentation methods had been applied; The relative threshold method, the Gradient method and the region growing method. Based on these results, we performed statistical analysis in two directions. 1. comparison of GTV and image segmentation results. 2. performance of regression analysis for relation between image factor affecting image segmentation techniques. The mean volume of GTV was $60.9{\pm}65.9$ cc and the $GTV_{40%}$ was $22.43{\pm}35.27$ cc, and the $GTV_{50%}$ was $10.11{\pm}17.92$ cc, the $GTV_{RG}$ was $32.89{\pm}36.8$4 cc, the $GTV_{GD}$ was $30.34{\pm}35.77$ cc, respectively. The most similar segmentation method with the GTV result was the region growing method. For the quantitative analysis of the image factors which influenced on the region growing method, we used the standardized coefficient ${\beta}$, factors affecting the region growing method show GTV, $TumorSUV_{MAX/MIN}$, $SUV_{max}$, TBR in order. The result of the region growing (automatic segmentation) method showed the most similar result with the CT based GTV and the region growing method was affected by image factors. If we define the tumor volume by the auto image segmentation method which reflect the PET image parameters, more accurate and consistent tumor contouring can be done. And we can irradiate the optimized radiation dose to the cancer, ultimately.