• Asian Pacific Journal of Cancer Prevention
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• v.16 no.8
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• pp.3301-3306
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• 2015

Nucleolin (C23) is an important anti-apoptotic protein that is ubiquitously expressed in exponentially growing eukaryotic cells. In order to understand the impact of C23 in radiation therapy, we attempted to investigate the relationship of C23 expression with the radiosensitivity of human non-small cell lung cancer (NSCLC) cells. We investigated the role of C23 in activating the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs), which is a critical protein for DNA double-strand breaks (DSBs) repair. As a result, we found that the expression of C23 was negatively correlated with the radiosensitivity of NSCLC cell lines. In vitro clonogenic survival assays revealed that C23 knockdown increased the radiosensitivity of a human lung adenocarcinoma cell line, potentially through the promotion of radiation-induced apoptosis and adjusting the cell cycle to a more radiosensitive stage. Immunofluorescence data revealed an increasing quantity of ${gamma}$-H2AX foci and decreasing radiation-induced DNA damage repair following knockdown of C23. To further clarify the mechanism of C23 in DNA DSBs repair, we detected the expression of DNA-PKcs and C23 proteins in NSCLC cell lines. C23 might participate in DNA DSBs repair for the reason that the expression of DNA-PKcs decreased at 30, 60, 120 and 360 minutes after irradiation in C23 knockdown cells. Especially, the activity of DNA-PKcs phosphorylation sites at the S2056 and T2609 was significantly suppressed. Therefore we concluded that C23 knockdown can inhibit DNA-PKcs phosphorylation activity at the S2056 and T2609 sites, thus reducing the radiation damage repair and increasing the radiosensitivity of NSCLC cells. Taken together, the inhibition of C23 expression was shown to increase the radiosensitivity of NSCLC cells, as implied by the relevance to the notably decreased DNA-PKcs phosphorylation activity at the S2056 and T2609 clusters. Further research on targeted C23 treatment may promote effectiveness of radiotherapy and provide new targets for NSCLC patients.

• Informatization Policy
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• v.18 no.4
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• pp.85-102
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• 2011

The world is focusing on e-books as the e-book market is growing rapidly as a fused industry at the crossroads of information technology and digital content. Many countries are actively participating in this new market and leveraging on its potential and growth, while Korea's market is still in its preliminary stage. There is a good reason to be concerned over the fact that access to digitalized materials that are the most relevant to the current time are not readily available. Therefore, the strategy in collecting and utilizing content must be newly refurbished. In order to do this, the legal issues and policies related to digital libraries must be considered. The most relevant examples from abroad are Google Book Search and the EU's Europeana project. Then, what are the necessary measures for Korea to materialize its own digital library? Digital libraries can significantly influence social welfare and bring forth economic effects. Social preparation surrounding this subject should continue to increase because digital libraries will change the manner in which people share and use information. Therefore, this paper aims to examine the legal and economic problems and issues related to the establishment of digital libraries as well as to provide policy recommendations.

• Journal of Korean Society of Forest Science
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• v.82 no.4
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• pp.347-353
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• 1993

Accumulation of air pollutants such as $SO_2$ and HF, chlorophyll contents, wax concentrations in needle tissue and needle growth of Pinus thunbergii were studied to verify possibilities of bioindicators of air pollution in Yochon Industrial Complex from July to October, 1991. The concentrations of water-soluble sulfur and fluorine increased nearer to the pollution sources regardless of sampling time, but water-soluble sulfur varied little during investigation and fluorine accumulated more with the passage of time. Total chlorophyll contents decreased a little in the slightly polluted site but sharply in the heavily polluted site. The higher the pollution level was, the more decreased chloroform-extractable wax. Air pollution exposure inhibited needle growth of Pinus tbunbergii during the latter part of growth period. Water soluble sulfur, fluorine and wax concentrations in needle of Pinus thunbergii were rather wood sensitive indicators of susceptibility to ambient air pollution from early July to early October. Chlorophyll contents seemed to be able to be used as a bioindicator of air pollution in early stage of needle development and in the severely polluted area. In the meanwhile needle length must be used as a bioindicator in latter part of growing season.

• Economic and Environmental Geology
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• v.36 no.5
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• pp.349-356
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• 2003

As industrial activities are growing, pollutants found in the contaminated land are getting diverse. Some contaminated areas are subject to mixed wastes containing both organic and inorganic wastes such as hydrocarbon and heavy metals. This study concerns with the influence of the degradation of organic pollutants on the coexisting heavy metals, expecially for As. As mainly exists as two different oxidation state; As(III) and As(V) and the conversion between the two chemical forms may be induced by organic degradation in the soil contaminated by mixed wastes. We operated microcosm in an anaerobic chamber for 60 days, using sandy loam. The soils in the microcosm are artificially contaminated both by tetradecane and As, with different combination of As(III) and As(V); As(III):As(V) 1:1, As(III) only and As(V) only. Although not systematic, ratio of As(III)/As(Total) increase slightly at the later stage of experiment. Considering complicated geochemical reactions involving oxidation/reduction of organic materials, Mn/Fe oxides and As, the findings in the study seem to indicate the degradation of the organics is connected with the As speciation. That is to say, the As(V) can be reduced to As(III) either by direct or indirect influence induced by the organic degradation. Although Fe and Mn are good oxidising agent for the oxidation of As(III) to As(V), organic degradation may have suppressed reductive dissolution of the Fe and Mn oxides, causing the organic pollutants to retard the oxidation of As(III) to As(V) until the organic degradation ceases. The possible influence of organic degradation on the As speciation implies that the As in mixed wastes may be have elevated toxicity and mobility by partial conversion from As(V) to As(III).

• Journal of Korea Technology Innovation Society
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• v.19 no.2
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• pp.254-279
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• 2016

Recently, with the conversion towards knowledge-based economy era, the importance of the evaluation for patent valuation has been growing rapidly because technology transactions are increasing with the purpose of practically utilizing R&D outcomes such as technology commercialization and technology transfer. Nevertheless, there is a lack of research on determinants of patent valuation by analyzing technology transactions due to the difficulty of collecting data in practice. Hence, to suggest quantitative determinants for the patent valuation which could be applied to scoring methods, 15 patent valuation models domestically and overseas are analysed in order to assure the objectiveness for subjective results from qualitative methods such as expert surveys, comparison assessment, etc. Through this analysis, the important 6 common determinants are drawn and patent information is matched which can be used as proxy variables of individual determinant factors by advanced researches. In addition, to validate whether the model proposed has a statistically meaningful effect, total 517 technology transactions are collected from both public and private technology transaction offices and analysed by multiple regression analysis, which led to significant patent determinant factors in deciding its value. As a result, it is herein presented that patent connectivity(number of literature cited) and commercialization stage in market influence significantly on patent valuation. The meaning of this study is in that it suggests the significant quantitative determinants of patent valuation based on the technology transactions data in practice, and if research results by industry are systematically verified through seamless collection of transaction data and their monitoring, we would propose the customized patent valuation model by industry which is applicable for both strategic planning of patent registration and achievement assessment of research projects (with representative patents).

• Journal of the Korean Society of Tobacco Science
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• v.7 no.1
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• pp.15-23
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• 1985

Growth response and evapotranspiration of tobacco as to each soil moisture condition treated with different height of ground water table such as 30cm, 100cm, 170cm, and 230cm were investigated. The results obtained were as follows; 1. Soil water potential in the rhizosphere (25cm depth from surface) treated with ground water table (G.W.T) of 230 cm was less than -6.5 bar at 60 days after transplanting. 2. Dry weight of leaves per plant was the highest in the pot treated with 100 cm G.W.T and was the lowest in the pot treated with 230 cm G.W.T.. Leaves/Stem ratio of dry weight was the lowest in the pot treated with 230 cm G.W.T. This would show that extension of leaves as compared with elongation of stem was suppressed. .3. In the roster stress condition of 230 cm G.W.T., extension of leaves was continued until the latter growth stage, Relative growth rate of leaf area per plant of the pot with 30 cm G.W.T. was the highest through all growth period after fixation of G.W.7., but owing to lower density of leaf, the dry weight of leaves per plant was less rather than that of the pot with 170 cm G.W.T. at 64 days after transplanting. 4. The highest evapotranspiration per plant per day was shown at the 45th day after transplanting in the pots with 30cm and 100cm G.W.T. and at 60 days in the pots with 170cm and 230cm G.W.T respectively. 5. In the soil moisture conditions with 30cm and 100cm G.W.T., evapotranspiration per plant per hour was much higher in the daytime that at night as well as in clear day than in rain-cloudy day owing to loner relative humidity , but in the water stress condition with 230 cm G.W.T., that was much lower. 6. Total evapotranspiration per plant during the whole growing period of 64 days was in order of 30cm > 100 cm > 170 cm > 230 cm G.W.T.. Total evapotranspiration for 34 days after fixation of G.W.T was much higher at night than in the daytime in case of water stress conditions i.e 170cm and 230cm G.W.T. but in normal condition of the pots with 30cm and 100cm G.W.T. that was much higher in the daytime than at night. 7. Leaf water potential of the 3rd and 6th leaf from top at 64 days after transplanting was in order of 100cm > 30 cm > 170cm > 230cm G.W.T. The reason why leaf water potential in the pot with 30cm was less than that in the pot with 100cm G.W.T. would be the abnormal turgidity with low density of cell substrates by the excess water absorption in the pot with 30cm G.W.T..

• Korean Journal Plant Pathology
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• v.11 no.1
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• pp.17-29
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• 1995

This study was conducted from 1991 to 1993 to develop a fungicidal spray program for the apples exportable to the United States of America, in which quarantine and pesticide residue in agricultural products are strictly regulated. In 1991, 2 spray schedules were applied to an orchard, in one of which the 7 fungicides registered for apple both in Korea and U.S. were used, and in the other of which the Bordeaux mixture for which the tolerance was exempted in U.S. was used 2 times along with those 7 fungicides. The apple white rot and fruit infection by Alternaria mali were not effectively controlled by the 7 fungicides alone; however, the control efficacy was raised by adding the Bordeaux mixture to the spray schedule. In 1992, 4 spray schedules were applied in which the kinds of fungicides and spray intervals were different one another. The results suggested that an effective spray program can be developed by adopting the Bordeaux mixture for 3 times or adopting the imminoctadine-triacetate which has not tolerance level in U.S. for same times in the vulnerable stage of apple white rot and alternaria blotch. In spite of the high efficacies against major apple diseases, the Bordeaux mixture could not be recommended to the apple growers due to the various defects such as restrictions in compatibility with insecticides and acaricides, troublesomeness in preparation and spray, especially the harmful effect on the finish of Fuji apples. In 1993, a spray program adopting 3 times of imminoctadine-triacetate during the growing season of apple was developed, which not only can effectively control the major apple diseases but also avoid the pesticide residue problems if it was sprayed 2 or 3 times after bagging. On the basis of the 3 years results, a basic fungicide spray program was formulated in which 1~3 times of imminoctadine-triacetate and 1~2 times of bitertanol were adopted in addition of the 7 common fungicides registered in both countries. In the results of application of the spray schedule to the actual farming in the 4 areas of Kyungpook Province in 1994, no noticeable defects were detected at the first year trial. However, this spray program will be continuously evaluated and modified to obtain better control efficacies against major apple diseases.

• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.4
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• pp.333-342
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• 2010

Corn is very important forage in Korea. The great part of them is utilized as silage. Generally, it contains a lot of grains that is feed of animal. This experiment was conducted to evaluation of fermentation ability of microbes for corn silage inoculant. Good lactic acid bacteria were isolated from good corn silage by plating MRS agar containing 0.02% sodium azide, and assessed by growing and acid producing ability in MRS broth. Six lactic acid bacteria were selected, and were found to be Gram positive, rods and catalase negative and were identified to be lactobacillus plantarum (C3-2, B13-1, CC9-1), Lactobacillus fermentum (C11-4), Lactobacillus paracasei (B14-1), and Leuconostoc lactis (A3-1) on the basis of the biochemical characteristics and utilization of substrates. Corn was ensiled at ripen stage following treatment with selected five lactic acid bacteria, two commercial inoculant, and no additive (control). After 2 month, B13-1 and CC9-1 bacteria inoculated silage were lower pH and higher lactic acid content than others treatments. The Flieg's score and grade of B13-1 and CC9-1 bacteria treated silage were higher than commercial inoculant. According to this experiment, lactobacillus plantarum B13-1 and CC9-1 strain were recommendable for good inoculant of corn silage.

• Journal of Animal Environmental Science
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• v.18 no.1
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• pp.25-34
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• 2012

Noxious gases with malodorous substance concentrations in each stages of pig buildings were determined at a typical 400sow-scale farm to improve piggery environment. Using IAQ-300 and pDR-1000AN, continuous records for the concentration of $NH_3$, CO, $CO_2$, $NO_2$, $SO_2$, $H_2S$, $O_2$, and along with temperature, humidity, dust concentrates from individual pig pens were collected to analyze every 6 hours' condition of indoor environment for 24 hours' period. In most pig houses, the air quality at noon was good, while at night (00:00~06:00), air composition became noxious in all buildings. The order of buildings' air quality for 24 hrs was pregnant > farrowing > nursery > growing > finishing. The cause of air quality differences was presumed to be the differences of stocking density, defecating amount and the length of exposure time of slurry in indoors. In conclusion, well-designed building structure, proper control of stocking density, quick removal of excreta from pig pens and continuous ventilation are prerequisites to improve pig housing environment.

• Korean Journal of Soil Science and Fertilizer
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• v.41 no.3
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• pp.177-183
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• 2008

Sodium is known to reduce a plant growth and yields. However, the relationships between physiological response of seedling and salinity stress caused by growing media are not well understood yet. We conducted experiments to investigate change of some parameters including Na, EC, moisture content in media under different air temperature ($15^{\circ}C$, $25^{\circ}C$), and the response of fruit-vegetables such as cucumber, oriental melon on saline conditions originated from horticultural substrate. Volumetric moisture content of media at $15^{\circ}C$ was 70%, but at $25^{\circ}C$ was decreased by 45% within 22 hrs, showing below optimal matric potential, approximately. During reaction time, the increase of Na concentration was significantly greater in saline substrate than in control. The decrease rate of Na concentration according to supplying irrigation water was higher in saline substrate than in control. $CO_2$ assimilation rate and transpiration rate of Korea melon grown in low temperature were decreased with a Na/cation ratio in hydroponic solution. Water saturation deficit was also increased significantly at $15^{\circ}C$ as compare to $25^{\circ}C$. Saline stress during nursery stage induced a reduction of seedling quality, growth and cucumber yield. The results suggest that the relationship between uncontrolled Na uptake of seedling from saline substrate and meteological condition is responsible for saline stress.