• 한국약용작물학회지
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• 제27권2호
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• pp.108-114
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• 2019

Background: Rutin is decomposed by rutin-degrading enzymes (RDE) during the processing of buckwheat groats, resulting in a decrease in rutin content and a further increase in the bitterness of processed products. Thus, the present study aimed to examine RDE activity in groats and various tissues of domestic buckwheat varieties and to develop a method to reduce the loss of rutin during the groat processing. Methods and Results: RDE activity and isozymes patterns were determined in Tartary and common buckwheat. RDE activity, measured by quercetin production rate, was 273 and $70{\mu}g/g$ fresh weight/min in mature Tartary and common buckwheat groats, respectively. A total of six RDE isozymes were detected in mature groats of Tartary buckwheat on a non-denaturing gel. In Tartary buckwheat groats, RDE activity decreased by approximately 81 or 71% with roasting or steaming for 5 min respectively. As the roasting or steaming time increased to 30 min, RDE activity decreased by over 95%. These results indicated that RDE was inactivated in groats by roasting or steaming. When untreated Tartary buckwheat groats were kneaded with powder, RDE was activated and the quercetin production rate increased by 62%. However, when roasted groats were kneaded with powder, the quercetin production rate decreased by 93%, mainly due mainly to inactivation of RDE, as indicated by a decrease in band intensities of the six isozymes. Conclusions: These results suggested that the loss of rutin, due to RDE activity during processing, may be reduced by 71 to 100% by roasting or steaming groats for 5 to 30 min, due in large part to the inactivation of RDE isozymes.

• 한국식생활문화학회지
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• 제23권1호
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• pp.121-129
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• 2008

To produce buckwheat sprouts, buckwheats were germinated at $23{\pm}2^{\circ}C$ up to 8 days in total darkness. In proximate analysis, moisture content increased from 13.87% of buckwheat groats to 93.75% of buckwheat sprouts on the 8th day of germination. On dry weight basis, lipid and ash contents increased like as protein content increased from 13.45% to 21.82% while carbohydrate content decreased due to enzyme hydrolysis. Amino acids were rich in glutamic acid, aspartic acids, and lysine, and also the ratio of the essential amino acids to total amino acids increased from 26.84% to 36.84%. Vitamin C did not exist in buckwheat groats but its content has continued to increase as far as 99.56 mg/100 g buckwheat sprouts. Rutin continued to increase by HPLC analysis from 4.71 mg/100 g buckwheat groats to 1,524 mg/100 g buckwheat sprouts. In analysis of organic acids, oxalic, maleic, and citric acids were commonly found in buckwheat sprouts. Fagopyrin was found almost none in buckwheat sprouts. In conclusion, the conversion of buckwheat seeds into sprouts through germination in darkness results in physically different final product with nutritional changes such as higher content of rutin, generation of vitamin C, abundance of the essential amino acids, and the existence of fagopyrin on very little.

• 한국작물학회지
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• 제50권spc1호
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• pp.175-180
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• 2005

본 연구는 단메밀과 타타리메밀의 페놀성화합물의 함량을 측정하고 분석, 평가한으로써 생리활성 연구의 기초 자료와 건강기능성 식품소재 개발에도 이용될 수 있는 유용한 기초 자료를 얻고자 수행하였다. 1. 총 페놀의 함량은 타타리메밀 종자가 단메밀 종자보다 약 2배정도 높게 나타났다. 총 페놀 중의 플라보노이드 함량은 단메밀이 약 $50\%$, 타타리메밀이 약 $95\%$를 차지하고 있다. 단메밀과 타타리메밀의 총 페놀 및 플라보노이드 함량은 메밀쌀이 메밀껍질보다 높았으나, 단메밀의 플라보노이드 함량은 메밀껍질 더 높았다. 3. 단메밀의 rutin함량은 메밀껍질(25.2 mg/100g)>껍질을 벗기지 않은 종자(19.8 mg/100 g)>메밀쌀(12.8 mg/100 g)의 순으로 높았으나, 타타리메밀은 메밀쌀(2042.1mg/100g)>껍질을 벗기지 않은 종자(1375.8 mg/100g)>메밀껍질(138.7 mg/100 g)의 순으로 높게 나타났다. 식물체 부위별 rutin의 함량은 단메밀이 메밀싹>잎>줄기>종자의 순이고, 타타리메밀은 잎enli메밀싹>종자>줄기의 순으로 나타났다. 모든 부위에서 타타리메밀이 단메밀보다 높은 rutin함량을 나타냈다. 4. 단메밀과 타타리메밀 종자의 flavanols함량은 큰 차이를 보이지 않았으나, 잎, 줄기, 싹나물부위의 함량은 타타리메밀이 높은 경향을 보였고, 두 종의 flavonols의 함량도 단메밀보다 타타리메밀에서 월등히 높은 경향을 보였다. 5. 식물체 부위별 flavanols의 함량은 단메밀과 타타리메밀의 메밀싹>잎>줄기>종자의 순으로 높게 나타났다. 모든 부위에서 catechin> Epicatechin> Epicatechingallate의 순으로 높게 나타났으나, 메밀싹은 두 종 모두 Epicatechingallate의 함량이 종자의 약 $30\~40$배, 잎, 줄기보다 약 $15\~20$배가량 높았다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.271-271
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• 2017

Tartary buckwheat is known for its high rutin (quercetin 3-rutinoside) content which has antioxidant, anti-inflammatory, and anticarcinogenic effects. The buckwheat tea which is popular in Korea, is dependent on the quality of applied processing methods (steaming, dehuling, and roasting). This study focused on the evaluation of changes in rutin and anti-oxidant contents during the processing of tartary buckwheat tea. Raw tartary buckwheat seeds contains the highest quantities of rutin (2,212 mg/100 g D.W.). Soaking in water and steaming the whole seeds of tartary buckwheat significantly decreased its rutin and quercetin contents. Whereas the contents of rutin and quercetin in dehulled groats increased after steaming. The process of roasting with $70-80^{\circ}C$ for 2-3 min significantly decreased the contents of rutin (992 mg/100 g D.W.) and quercetin (12.8 mg/100 g D.W.). In the processing of tartary buckwheat tea, rutin content dropped about 45% in comparison with raw whole seeds.

• 한국식품과학회지
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• 제22권7호
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• pp.732-737
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• 1990

HPLC를 이용하여 메밀종자 및 메밀식품에서의 rutin 함량을 분석하였다. 메밀종자의 rutin 함량은 재래종과 신농1호 사이에 상당한 차이가 있었으나. 신농1호 품종간 즉, 여름형과 가을형간에는 차이가 없었다. 가식부분인 메밀쌀의 rutin 함량은 여름형 신농1호에서 가장 높았다. 건조메밀국수와 막국수의 rutin 함량은 시료에 따른 차이가 상당히 컸었으며, 이와 같은 차이는 메밀국수 제조시 밀가루를 $10{\sim}50%$ 혼합하는데 기인하는 것으로 생각되었다. 메밀전병은 제품의 표시에 명기된 메밀함량으로부터 계산된 값보다 높은 rutin 함량을 나타내었다.

• 한국작물학회지
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• 제24권4호
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• pp.38-44
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• 1979

연맥품종 Pennfield의 등숙기동안 이삭의 $^14$C-sucrose 침지, $^14$$CO_2$의 지엽 또는 이삭 조사로서 동화물질의 source를 달리하였을 때 grain sink의 기능을 종실성숙의 정도, 소수의 수, 종실의 위치를 비교하여 검토하고, 절엽 및 제영처리를 하여 source와 sink간의 균형을 변화시켰을 때 종실중에 미치는 영향을 조사하였다. 1. 절취된 이삭의 하부에 $^14$C-sucrose를 침지하였을 때 종실로 $^14$C물질의 전류는 개화후 15일까지 직선적으로 상승하고 18일까지 급격히 하락하여 종실중의 증가보다 빨리 시작하고 먼저 끝나는 경향을 보였다. 하위절위 종실이 상위절위보다 $^14$C의 전류가 높았는데 이는 등숙의 진행이 늦은 때문이었으며 일차영화는 이차영화보다 높은 sink의 기능을 보였다. 2. 식물체에$^14{CO}_2$를 조사하였을 경우 이삭조사가 지엽조사보다 전류률이나 종실당 specific activity에서 3배 이상 높게 나타나 이삭이 종실의 등숙에 보다 효과적인 source임을 보여주었다. 종실당 specific activi쇼는 sink인 소수의 수의 영향보다 source의 종류에 따라 크게 달라졌으며 source의 activity가 큰 순서인 이삭의$^14$C-sucrose 침지 > 이삭의 $^14$$CO_2$ 조사 > 지엽의 $^14$$CO_2$ 조사 순으로 나타났다. 3. 절엽 및 제영처리로서 source/sink ratio를 변화시켰을 때 source/sink ratio가 감소함에 따라 종실중이 감소하였으며 엽면적과 소수의 수를 동시에 반감하였을 때 정상식물의 종실중과 차이가 없었다. 모든 잎을 제거하였을 때도 종실중에서 16% 감소에 불과하여 이삭의 동화작용이 크게 기여하는 것으로 보인다.

• 한국식품조리과학회지
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• 제10권3호
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• pp.249-253
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• 1994

In this study, total dietary fiber contents, acid detergent fiber contents lignin, water binding capacity (WBC), iron binding capacity of ADF and rutin contents from Korean buckwheats were determined. Total dietary fiber (TDF) content was 3.14% in kyungbuk rice buckwheat, 5.65% in kangwon hull buckwheat and 3.35% in kangwon rice buckwheat. Acid detergent fiber (ADF) content was 2.47, 4.46 and 2.94%, respectively and lignin content was 1.14, 1.60 and 1.08% respectively. The water binding capacity of these three sample dietary fiber showed that TDF were in the range of 2.87~3.88 g %H_2O$/g DF and ADF were in the range of 4.62~5.26 g %H_2O$/g DF. The iron binding capacity of ADF at pH 5.0, 6.0, 7.0 were 79.11, 78.01, 46.16% in kyungbuk rice buckwheat, 70.63, 63.83, 53.60% in kangwon hull buckwheat and 77.67, 76.33, 50.25% in kangwon rice buckwheat. The rutin contents of these samples and their hulls were 13.54~16.41 mg/100 g groats and 12.13~14.24 mg/100 g in hulls. The rutin content of kyungbuk rice buckwheat was the highest.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2002년도 심포지엄
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• pp.43-46
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• 2002

The first record of buckwheat in Korea was revealed from an old Korean book, "Hyangyakguguebbang(鄕藥救急方)" written in the era of king 'Gojong' who governed the country from AD 1236 to 1251 in the Korea dynasty. Buckwheat in Korea has been known to be introduced from China in 5th -6th century. One of the most famous buckwheat noodles, 'Naengmyeon' had been used popularly among the people in the Korea dynasty. It is true that buckwheat had been cooked and sold in temples during the Korea dynasty, and was regarded it as one of the temple foods at that time. Buckwheat has been cultivated as an alternative crop and buckwheat grains have been used as a hardy plant when the food situation for people was lacking in Korea. Buckwheat was an important crop in a slash-and-burn field(shifting cultivation) of the mountainous area in Kangwon province. A written history of the shifting cultivation in Korea was found in an old book "Taekriji" which was written by Lee, Jung-Hwan in the Chosun dynasty. The area and number of households of shifting cultivation in Korea was 40,000 ha and 135,000 household, respectively in 1973. Fifty to sixty kilogram of buckwheat grains per la was harvested at an altitude of 600 - 800m. Folk songs which have been sung among the people in shifting areas include buckwheat meal. One of the folk songs for buckwheat is as follows; "they do not eat rice with or without waxy property but eat buckwheat mixture." Since 1974, shifting cultivation has been legally prohibited to allow revegetation of destroyed mountains and rehabilitation of woodlands in Korea. Buckwheat has been traditionally marketed as noodle or flour for the manufacture of noodles, and also as groats for food in Korea. Consumption in markets has increased over the past several years. Buckwheat grains are mainly consumed in the form of noodles in Korea. However, many people have recently pursued a desire to make food products of high quality in processing buckwheat materials for health reasons.

• 한국식품과학회지
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• 제54권2호
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• pp.185-195
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• 2022

메밀의 식품가공기술 개발을 통해 이용성을 확대하고 새로운 소비수요를 창출하고자 일반메밀과 쓴메밀을 대상으로 가공단계 및 추출방법에 따른 메밀 볶음차의 품질 특성을 비교 분석하였다. 일반메밀의 원료, 탈피, 찌기 및 볶음 단계의 루틴 함량은 21.6, 17.8, 9.8 및 6.8 mg/100 g으로 차 가공공정이 진행됨에 따라 유의하게 감소하였다. 쓴메밀의 원료, 찌기, 탈피 및 볶음 과정의 루틴 함량은 1,679.9, 1,044.2, 703.1 및 834.5 mg/100 g으로 감소하였으나, 퀘세틴 함량은 0, 12.3, 36.0 및 32.3 mg/100 g으로 가공공정이 진행됨에 따라 증가하였다. 메밀차는 메밀의 종류, 제형 및 추출방법에 따라 루틴 함량에서 큰 차이를 보였다. 루틴 함량은 일반메밀차보다 쓴메밀차에서 평균 61배 높았다. 관능평가 결과를 종합하면, 쓴메밀차의 제형별 비교에서는 알곡보다 분말이 우림색, 풍미, 구수한 맛, 단맛 및 선호도에서 높은 값을 보였다. 특히, 루틴은 퀘세틴이나 쓴맛과 높은 상관관계를 나타냈다. 본 연구에서는 쓴메밀 품종인 '황금미소'를 대상으로 쓴맛을 최대한 줄이면서 루틴 함량이 높은 메밀차 제조를 위해 볶음 과정의 열처리 조건을 70-80℃에서 2-3분으로 확립하였다. 루틴 함량이 많은 메밀차를 섭취하고자 할 경우에는 핸드드립으로 추출한 방법이 적합하며, 쓴맛이 적고 달콤한 맛과 풍미가 좋은 메밀차를 원할 경우 침출식 콜드브루 방식을 선택하는 것이 가장 유리할 것으로 판단된다.