• 환경위생공학
• /
• 제3권1호
• /
• pp.1-8
• /
• 1988

It has been reported that the pulmonary mycosis generally results from saprophytic colonization of pre-existing lung cavities usually due to pulmonary tuberculosis etc. Culture of homogenized tissues yield pure colony of slightly brown on the Sabouraud dextrose medium. The fungus was identified as the Scophulariopsis brevieaulis which had been rarely reported in pulmonary fungal infection. Histopathological examination of excised tissues revealed a chronic granulomatous inflammation and many branched hyphal mass. Susceptibility tests were tested with ketoconazole, griseofulvin, nystatin, and amphotericin B. Of these antifungal agents, ketoconazole and griseofulvin proved to be most effective and minimum inhibitory concentration was $10{\mu}g/ml,\;50 {\mu}g/ml$, respectively.

• 한국식물병리학회지
• /
• 제14권6호
• /
• pp.700-704
• /
• 1998

A total of 26 and 55 isolates of fungi were isolated from corn and wheat samples collected from different markets in Korea, respectively. The number of Penicillium isolates from corn and wheat was 9 and 33, respectively. The Penicillium species isolated from corn were P. chrysogenum (3 isolates) and P. oxalicum (6 isolates), and from wheat were P. aurantiogriseum (16 isolates), P. citrinum (1 isolate), P. commun (4 isolates), P. griseofulvum (1 isolate), P. verrucosum (7 isolates), and P. viridicatum (4 isolates). Production of major mycotoxins in the yeast extract sucrose medium cultures of Penicillium isolates was analysed. Penicillium cultures were extracted with chloroform and purified by thin-layer chromatograhy (TLC), and high performance liquid chromatography (HPLC). Among 9 isolates of Penicillium from corn, 2 isolates of P. chrysogenum produced patulin, 1 isolate of the fungus produced patulin and citrinin, 2 isolates of P. oxalicum produced penicillic acid, 4 isolates produced pencillic acid and griseofulvin. Of the 33 isolates of Penicillium from wheat, 6 isolates of P. aurantiogriseum produced patulin, 8 isolates produced penicillic acid, 1 isolate produced patulin and penicillic acid, 1 isolate of P. citrinum produced citrinin and patulin, 2 isolates of P. commun produced brefeldin A and patulin, 1 isolate of P. griseofulvum produced brefeldin A, griseofulvin and patulin. Five isolates of P. verrucosum produced patulin, 1 isolate of the fungus produced penicillic acid, and 3 isolates of P. viridicatium produced penicillic acid.

• 폴리머
• /
• 제33권6호
• /
• pp.515-519
• /
• 2009

생분해성 고분자인 poly((R)-3-hydroxy butyrate)와 poly(ethylene glycol)을 결합시켜 양친성 이중블록 공중합체를 형성하었고, 표적인자인 folate를 수식하여 특정 암세포에 표적화하도록 설계하였다. 이 공중합체는 수용액상에서 미셀을 이루며, DLS로 측정한 결과, 125~156 nm의 크기였고, 동결건조하여 SEM으로 관찰한 결과 구형임을 확인하였다. 여기에 소수성 약물인 griseofulvin을 사용하여, 35~56%의 봉입률을 나타내었다. 약물은 in vitro상에서 24시간 동안 지속적으로 방출되었다. 세포생존율을 측정하여, folate가 수식된 입자가 그렇지 않은 입자보다 약 10% 더 낮은 세포생존율을 보임으로써 표적지향 효과가 있다는 것을 알 수 있었다.

• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 2000년도 추계 학술발표회
• /
• pp.40.1-40
• /
• 2000

• 분석과학
• /
• 제12권6호
• /
• pp.534-539
• /
• 1999

Penicillium, Aspergillus 및 Fusarium에 오염된 밀(Triticum aestivum L.), 콩(Glycine max Merr), 옥수수(Zea mays L.)에서 HPLC를 이용하여 주요 독소들을 검정하였다. Penicillium 독소인 brefeldin A는 밀에서 3.1~270 ppm, 콩에서 45~230 ppm, 옥수수에서 1030~1240 ppm 다량 검출되었다. Citreoviridin과 griseofulvin은 밀, 콩, 옥수수에서 각각 40~80 ppm과 3.6~26.0 ppm이 검출되었다. 그리고 citrinin과 patulin은 밀, 콩, 옥수수에서 각각 0.3~4.0 ppm과 420~3800 ppm로 검출되었고, 특히 옥수수에서 다량 검출되었다. Aspergillus 독소로 ochratoxin A는 밀에서 730 ppm, 콩에서 12.4 ppm, 옥수수는 310 ppm이 검출되었다.

• Macromolecular Research
• /
• 제16권5호
• /
• pp.418-423
• /
• 2008

Poly[(R)-3-hydroxy butyrate] (PHB) and methoxy poly(ethylene glycol) (mPEG) were conjugated by the transesterification reaction with tin(II)-ethylhexanoate (Sn(Oct)-II) as a catalyst. Hydrophobic PHB and hydrophilic mPEG formed an amphiphilic block copolymer which was formed with the self-assembled polymeric micelle in aqueous solution. In this study, we tried to determine the optimum ratio of hydrophobic/hydrophilic segments for controlled drug delivery. The particle size and shape of the polymeric micelle were measured by atomic force microscopy (AFM) and transmission electron microscopy (TEM). Their size were 61-102 nm with various block ratios. Griseofulvin was loaded in the polymeric micelle as a hydrophobic model drug. The loading efficiency and release profile were measured by high performance liquid chromatography (HPLC). The model drug in our system was constantly released for 48 h.

• 대한화학회지
• /
• 제52권1호
• /
• pp.30-35
• /
• 2008

연구의 목적은 N1과 6번 자리에 다른 치환기를 가진 5-cyano가 치환된 pyrimidine 아날로그의 새로운 시리즈의 합성과 그것들의 항균성과 항진균성의 활성도에 대해 평가하기 위해서이다. 이 화합물은 MORE technique 를 이용한 potassium carbonate 의 존재하에서 ethylcyanoacetate, 치환된 thioureas, 적당한 알데히드의 제 3차 축합으로 합성 되어졌다. 항균성과 항진균성의 활성도는 25 mg의 농도에서 cup-plate 방법으로 측정되었다. 억제구역은 mm로 측정 되어졌다. 모든 화합물은 좋은 항균성과 항진균성을 보여주었다. P1과 P5는 S.aureus과 E.coli에 대해 최대 활성도를 보여주었고, P6은 모든 종류의 미생물에 대해 좋은 활성도를 보여주었다. P8 화합물은 C. albicans 에 대해 좋은 효과가 있음을 알아냈다. Norfloxacin와 griseofulvin는 합성된 화합물의 활성도와 비교되는 기준물질로 사용되었다. 6번 자리에 p-hydroxy와 p-methoxy로 치환된 phenyl moiety를 가진 gram-양성 미생물에 대해 강력했고, 6번 자리에 이것들이 없는 phenyl moiety 를 가진 아날로그는 gram-음성 활성도를 가졌다,6번 자리에 p-dimethylamino로 치환된 phenyl moiety를 가진 화합물은 적당한 활성을 보여준다.. 게다가 N1자리에서 단지 fluorine을 포함한 아날로그는 상당한 항진균성을 가졌음을 밝혀냈다. N1자리에서 aryl moiety 의 전자 끌게 치환과 마찬가지로 전자 주게 치환은 화합물의 결정된 능력에 중요한 역할을 함을 제시한다.

• 한국동물위생학회지
• /
• 제26권1호
• /
• pp.57-65
• /
• 2003

The present study was conducted to examine the outbreak state of bovine dermatophytosis in 14 farms(4 dairy farms, 10 Korean indigenous cattle farms) in Gyeongbuk province from November 2000 to November 2001. The causative agents of dermatophytosis was identified by mycological examination. Antifungal susceptibility test of 26 isolates was performed by agar dilution method, using 5 antifungal drugs. Prevalence of bovine dermatophytosis was found to be 13.5%(90/665) in dairy cattle farms and 14.5%(220/1,520) in Korean indigenous cattle farms. The most common age at which this disease occurred was 2-12 months. This disease usually occurred from winter to spring and the occurrence subsequently decreased in the summer. But 4 Korean indigenous cattle farms with poorly hygienic status were occurred all the year round. The causative agent was identified as Trichophyton verrucosum exclusively in these case. Antifungal susceptibility test of T verrucosum (26 strains) was performed by agar dilution method, using 5 antifungal drugs including tolnaftate, griseofulvin, ketoconazole, amphotericin B and terbinafine. All isolates were highly sensitive to 5 antifungal drugs (geometric mean MICs 0.004∼0.032 $\mu\textrm{g}$/$m\ell$). The isolates were the most sensitive to especially tolnaftate.

• 한국균학회소식:학술대회논문집
• /
• 한국균학회 2015년도 춘계학술대회 및 임시총회
• /
• pp.20-20
• /
• 2015

Endophytic fungi are microorganisms inhabiting living plant tissues without causing apparent harm to the host. They are drawing increasing attention due to their ability to produce various bioactive compounds as well as their effects on host growth and resistance to biotic and abiotic stresses. As a first step to assess biodiversity of plant associated fungi in Korea and the following evaluation on diverse biological activities, we are collecting endophytic fungi from plant in wild followed by systematic long-term storage in liquid nitrogen. Molecular identification using ITS sequences was also incorporated for pure culture by hyphal tip isolation. As of April 2015, about 1,400 fungal strains had been isolated from about 170 plant taxa. Fungal isolates belonging to Pleosporales, Diaporthales, Glomerellales, Hypocreales, and Xylariales were the most abundant. These collections are being used for several complementary researches, including screening of isolates with novel bioactive compounds or conferring drought stress resistance, phylogenetic and genomic study. Genome sequencing was performed for 3 isolates, one Xylaria sp. strain JS573 producing griseofulvin, an antifungal compound, and two Fusarium spp. strains JS626 and JS1030, which are assumed to be new species found in Korea. More detailed analysis on these genomes will be presented. These collections and genome informations will serve as invaluable resources for identifying novel bioactive materials in addition to expand our knowledge on fungal biodiversity.

• Macromolecular Research
• /
• 제15권6호
• /
• pp.547-552
• /
• 2007

Water soluble polymer, poly(vinyl pyrrolidone) was chosen to conjugate with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(succinyl) (N-succinyl DPPE) to make a new drug delivery system. PVP with an amine group (amino-PVP) was polymerized by free radical polymerization. The amine group of amino-PVP was conjugated with the carboxylic group of N-succinyl DPPE. The resultant conjugate could form nanoparticles in the aqueous solution; these nanoparticles were termed a lipid-polymer system. The critical aggregation concentration was measured with pyrene to give a value of $1{\times}10^{-3}g/L$. The particle size of the lipid-polymer system, as measured by DLS, AFM and TEM, was about 70 nm. Lipophilic component in the inner part of the lipid-polymer system could derive the physical interaction with hydrophobic drugs. Griseofulvin was used as a model drug in this study. The loading efficiency and release profile of the drug were measured by HPLC. The loading efficiency was about 54%. The release behavior was sustained for a prolonged time of 12 days. The proposed lipid-polymer system with biodegradable and biocompatible properties has promising potential as a passive-targeting drug delivery carrier because of its small particle size.