• Bulletin of the Korean Chemical Society
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• v.34 no.11
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• pp.3289-3294
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• 2013

An efficient method for the preparation of 8-benzylidene-4-phenyl-3,4,5,6,7,8-hexahydro-1H-quinazolin-2-ones/thiones from the reaction of aromatic aldehydes with cyclohexanone and urea or thiourea in the presence of Tetrabutylammonium hexatungstate, $[TBA]_2[W_6O_{19}]$, as an efficient, inexpensive catalyst under thermal and solvent-free conditions has been developed. Good yields, short reaction times, straightforward workup, reusability of the catalyst, and green conditions are the most obvious advantages of this procedure.

• Communications for Statistical Applications and Methods
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• v.29 no.3
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• pp.301-317
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• 2022

In this paper, we propose a change point detection procedure based on the modified information criterion in a generalized lambda distribution (GLD) model. Simulations are conducted to obtain empirical critical values of the proposed test statistic. We have also conducted simulations to evaluate the performance of the proposed methods comparing to the log-likelihood method in terms of power, coverage probability, and confidence sets. Our results indicate that, under various conditions, the proposed method modified information criterion (MIC) approach shows good finite sample properties. Furthermore, we propose a new goodness-of-fit testing procedure based on the energy distance to evaluate the asymptotic null distribution of our test statistic. Two real data applications are provided to illustrate the use of the proposed method.

• Bulletin of the Korean Chemical Society
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• v.33 no.5
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• pp.1556-1560
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• 2012

A simple highly versatile and efficient method has been developed for the three-component coupling of aldehydes, amines and alkynes to prepare propargylamines, in the presence of a catalytic amount of $BiCl_3$. The advantages of methods are high yield, mild reaction conditions, no environmental pollution and easy work up procedure.

• Journal of the Korean Chemical Society
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• v.52 no.5
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• pp.523-529
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• 2008

• Bulletin of the Korean Chemical Society
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• v.34 no.10
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• pp.2963-2967
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• 2013

This paper describes a simple and efficient method involving domino Knovenegal/$6{\pi}$ electrocyclization for the preparation of a variety of 2H-pyrans using microwave irradiation under solvent- and catalyst-free conditions. This method offers the advantages of a green approach, high yields, and short reaction times. Sixteen compounds (9a-p) were obtained in good to excellent yields using the procedure.

• Bulletin of the Korean Chemical Society
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• v.32 no.1
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• pp.225-228
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• 2011

Poly(4-vinylpyridine)-supported $Br{\phi}nsted$ acids (P4VP-HX) were prepared by wet impregnation technique. These supported acids were found as efficient heterogeneous green catalysts for acetylation of alcohol, amine and phenol with different catalytic activities. The wide application of P4VP-HX as reusable solid acid catalyst in organic reactions is possible because of its simple preparation and handling, stability, simple work up procedure.

• Bulletin of the Korean Chemical Society
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• v.13 no.5
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• pp.538-541
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• 1992

An approximate scheme for evaluating total reaction probability is proposed. Semiclassical boundary conditions are imposed well before the asymptotic region in the reactant and product channels to calculate the Green's function and its derivatives. Propagations are confined to a limited regime near the activated complex. Calculations are made for one dimensional Eckart barrier model of H + $H_2$ reaction. Implications of the procedure in multi-dimensional systems are discussed.

• Journal of Korean Ophthalmic Optics Society
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• v.13 no.1
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• pp.119-124
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• 2008

Purpose: To determine whether the accommodation of amplitude (AA) was changed by the color of the spectacle lens or object. Methods: AA was measured in forty subjects in their 20s when they viewed different targeton-background color combination with achromatic, gray, brown or green lens. Minus-lens procedures were used for the estimation of AA. Results: When subjects viewed the black-on-white, red-on-white and green-on-white targets, AA under tinted lens tended to be increased compared with AA under achromatic lens. Especially, the green lens significantly increased AA whatever the color of target was. Furthermore, as subjects viewed the green target, AA was the highest irrespective of the color of lens. AA was also changed depending on the color of background, so AA on the red background was lower than on the white background. On the contrary, AA on the green background was higher than on the red or white backgrounds. Of tinted lens, the gray lens increased AA the lowest, but the green lens did the highest. The number of subjects, whose AA were measured more than 9 D, reached to 12.5% with the gray lens, 21.3% with the brown lens, 22.5% with the green lens on the green background, but 5%, 6.5% and 6.5% on the red background, respectively. Conclusions: This results showed that AA varied depending on the color of spectacle lens, objects or background, and the eye fatigue could be decreased with proper color of spectacle lens accordingly.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.5
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• pp.743-748
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• 2017

Objective: Based on rapid advancement of genetic modification techniques, genomic editing is expected to become the most efficient tool for improvement of economic traits in livestock as well as poultry. In this study, we examined and verified the nickase of mutated CRISPR-associated protein 9 (Cas9) to modulate the specific target gene in chicken DF1 cells. Methods: Chicken myostatin which inhibits muscle cell growth and differentiation during myogenesis was targeted to be deleted and mutated by the Cas9-D10A nickase. After co-transfection of the nickase expression vector with green fluorescent gene (GFP) gene and targeted multiplex guide RNAs (gRNAs), the GFP-positive cells were sorted out by fluorescence-activated cell sorting procedure. Results: Through the genotyping analysis of the knockout cells, the mutant induction efficiency was 100% in the targeted site. Number of the deleted nucleotides ranged from 2 to 39 nucleotide deletion. There was no phenotypic difference between regular cells and knockout cells. However, myostatin protein was not apparently detected in the knockout cells by Western blotting. Additionally, six off-target sites were predicted and analyzed but any non-specific mutation in the off-target sites was not observed. Conclusion: The knockout technical platform with the nickase and multiplex gRNAs can be efficiently and stablely applied to functional genomics study in poultry and finally adapted to generate the knockout poultry for agribio industry.

• Korean Journal of Food Science and Technology
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• v.11 no.2
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• pp.77-80
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• 1979

Effects of interfering substances on the determination of L-ascorbic acid (AsA) in green tea and it's extracts by 2,4-dinitrophenylhydrazine (DNP) method was studied. and the removal of these interfering substances was also investigated. Under the condition prescribed for DNP method, AsA content of green tea are effected by some sugar, reductones, dicarbonyl compounds, organic acids, amino acids and others. All interfering substances except amino acids were eliminated by the chloroform extraction after adding o-phenylendiamine to sample solution. and remaining amino acids were eliminated almost completely by the treatment with ion exchange resin$(Amberlite{\;}IR-120H^{+})$. After removing the interfering substances by the above mentioned procedure, total AsA in green tea was determined by DNP method. The values obtained by this method were in good agreement with those by thin layer chromatography (TLC) method. and the method was more rapid and simple than TLC method.