• Asian Pacific Journal of Cancer Prevention
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• v.17 no.4
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• pp.2235-2245
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• 2016

Ulcerative colitis (UC) results from colonic epithelial barrier defects and impaired mucosal immune responses. In this study, we aimed to investigate the modifying effects of a Spirogyra neglecta extract (SNE), a polysaccharide extract (PE) and a chloroform fraction (CF) on dextran sodium sulfate (DSS)-induced colitis in mice and to determine the mechanisms. To induce colitis, ICR mice received 3% DSS in their drinking water for 7 days. Seven days preceding the DSS treatment, oral administration of SNE, PE and CF at doses of 50, 25 and 0.25 mg/kg body weight (low dose), 200, 100 and 1 mg/kg body weight (high dose) and vehicle was started and continued for 14 days. Histologic findings showed that DSS-induced damage of colonic epithelial structure and inflammation was attenuated in mice pre-treated with SNE, PE and CF. Furthermore, SNE and PE significantly protected colonic epithelial cells from DSS-induced cell cycle arrest, while SNE, PE and CF significantly diminished apoptosis. Proteome analysis demonstrated that SNE and PE might ameliorate DSS-induced colitis by inducing antioxidant enzymes, restoring impaired mitochondria function, and regulating inflammatory cytokines, proliferation and apoptosis. These results suggest that SNE and PE could prevent DSS-induced colitis in ICR mice by protection against and/or aiding recovery from damage to the colonic epithelium, reducing ROS and maintaining normal mitochondrial function and apoptosis.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.51 no.3
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• pp.262-277
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• 2018

We seasonally examined marine macroalgal community structures and ocean environmental characteristics in subtidal zones at five sites on and around Jeju Island, Korea, from February 2013 to November 2015. A total of 186 macroalgal species were identified, including 18 green, 33 brown, and 135 red algae. During the study period, the number of species was highest at Gapado Island (125 species) followed by Bukchon (123 species), Sagye (122 species), Sinheung (122 species) and Sinchang (97 species). Ecklonia cava, Peyssonnelia caulifera, Synarthrophyton chejuense, Corallina aberrans and Corallina crassisima occurred at all study sites and in all seasons. The average annual biomass of seaweed was $1,125.10g\;wet\;wt./m^2$ and ranged from $899.77g/m^2$ at Sinheung to $1,452.00g/m^2$ at Gapado. A brown alga E. cava was the most dominant species, accounting for 49.84% ($560.78g/m^2$) of the total seaweed biomass. Subdominant species were C. aberrans and C. crassissima, comprising 6.83% ($76.79g/m^2$) and 5.98% ($67.28g/m^2$) of total biomass, respectively. Cluster analysis revealed three distinct groups: the Sagye/Sinheung group (group A), the Bukchon/Sinchang group (group B), and the Gapado group (group C), indicating significantdifferences in macroalgal communities between sites.

• Journal of Microbiology and Biotechnology
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• v.15 no.1
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• pp.131-135
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• 2005

Under sulfur deprived conditions, PS II and photosynthetic $O_2$ evolution by Chlamydomonas reinhardtii UTEX 90 are inactivated, resulting in shift from aerobic to anaerobic condition. This is followed by hydrogen production catalyzed by hydrogenase. We hypothesized that the photosynthetic capacity and the accumulation of endogenous substrates such as starch for hydrogen production might be different according to cell age. Accordingly, we investigated (a) the relationships between hydrogen production, induction time of sulfur deprivation, increase of chlorophyll after sulfur deprivation, and residual PS II activity, and (b) the effect of initial cell density upon sulfur deprivation. The maximum production volume of hydrogen was 151 ml $H_2$/l with 0.91 g/l of cell density in the late-exponential phase. We suggest that the effects of induction time and initial cell density at sulfur deprivation on hydrogen production, up to an optimal concentration, are due to an increase of chlorophyll under sulfur deprivation.

• Korean Journal of Environmental Biology
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• v.17 no.3
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• pp.315-319
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• 1999

Spore attachment of Ulva fasciata on agar and agaroses coating films showed various surfaces properties according to concentrations of agar or agaroses or via methanol. The highest number of spore attachment occurred in Sigma agarose coating film. Spore attachment on Bacto agar and other agaroses coating films showed the 12 ∼ 36 times less than Sigma agarose coated film. Comparison of spore attachment on 2.5% and 5％ Seakem agarose and Bacto agar coating films differed in two concentrations while coating of 2.5% and 5％ with NuSieve and Sigma agaroses did not differed. Spore attachment of coating made of 5％ and 5% via 4% MeOH with Bacto agar and Nusieve agarose only differed. Overall, these results indicated agar and agarose coating films differed in spore attachment. Results of this work will be useful baseline for bioassay of antifouling activity of fouling organisms.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.38 no.3
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• pp.164-171
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• 2005

Codium fragile (Suringar) Hariot, an edible green alga is farmed in Korea by natural blooming zygotes attachment. Experiments were conducted to reveal the conditions for artificial seed production of C. fragile by sexual and asexual reproduction. Growth was compared between zygotes attachment (sexual reproduction) and isolated utricles with medullary filaments (asexual reproduction). Zygotes and isolated utricles with medullary filaments were cultured under different light conditions (10, 20, 40, 60 and $100\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and temperatures (5, 10, 15, 20 and $25^{\circ}C$) under 16:8LD. Maximum growth of zygote was $261.3{\pm}21.0\;{\mu}m$ under $15^{\circ}C$ and $20\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ after 13 days culture. Maximum regeneration of isolated medullary filament was $8.1{\pm}1.7\;mm$ per one isolated utricle under $20^{\circ}C$ and $100\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ after 15 days culture. After intermediate culture during two months in the field, morphogenesis occurred in both sexual and asexual reproduction, and growth of young thalli was not significantly different (p>0.05) between the both reproduction methods. Even though seed production of C. fragile is possible in both sexual and asexual reproduction, the mass artificial seed production of asexual reproduction is much more effective than that of sexual reproduction that is too much affected by maturity.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.6
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• pp.658-664
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• 2011

Rotifers of the genus Brachionus are commonly used as a live food for larval fish, and rotifers of different sizes are preferred according the mouth size of the fish. Rotifer species vary in size, and individual size can depend on the temperature and salinity of the rearing environment. We investigated the effects of temperature and salinity for two species, B. plicatilis (250-300 ${\mu}m$) and B. rotundiformis (100-220 ${\mu}m$). Two strains of B. plicatilis (CCUMP 36 and 48) and two strains of B. rotundiformis (CCUMP 51 and 56) were received from the Culture Collection of Useful Marine Plankton (CCUMP) at Pukyong National University and cultured with the green alga, Nannochloris oculata (KMMCC 16) from the Korea Marine Microalgal Culture Center (KMMCC). The growth and size of rotifers were examined at three water temperatures ($16^{\circ}C$, $24^{\circ}C$, $32^{\circ}C$) and four salinities (20 psu, 25 psu, 30 psu, 35 psu) under continuous light (40 ${\mu}molm^{-2}s^{-1}$). The maximum density and growth rate of B. rotundiformis were greater than those of B. plicatilis. The lorica length of B. plicatilis ranged from 215.4 to 269.7 ${\mu}m$ and from 154.9 to 206.6 ${\mu}m$ for B. rotundiformis, depending on strain, temperature and salinity. Rotifers were smaller when cultured at high temperatures, regardless of salinity. B. rotundiformis preferred higher salinity than B. plicatilis. The results demonstrated that the size of rotifers could be controlled to some extent by temperature and salinity.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.6
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• pp.740-746
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• 2011

Subtidal benthic macroalgal flora and community structure on barren grounds were examined seasonally along vertical shore gradients on the rocky shore of Hajung, Pohang, on the southeast coast of Korea, from February 2005 to November 2006. Twenty-six seaweed species were identified, including 5 green, 7 brown, and 14 red algae. The number of seaweed species ranged between 7 and 17 among seasons and between 13 and 20 species were found in vertical gradients along the shore. Over the study period, average seaweed biomass (g wet wt/$m^2$) was 299.88 g and it ranged seasonally from 120.99 to 620.00 g. Seaweed biomass declined with increasing seawater depth and ranged between 323.06 and 593.68 g. The dominant seaweed species, in terms of biomass, were Desmarestia ligulata and Sargassum honeri, which grew at depths between 5 and 10 m. The red alga Delisea pulchra was also abundant at a depth of 15 m. No seasonal patterns were found in community indices. Along vertical shore gradients, community indices showed different patterns; the dominance index increased and the richness, evenness, and diversity indices decreased with seawater depth.

• Preventive Nutrition and Food Science
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• v.15 no.3
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• pp.206-212
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• 2010

Codium fragile (CF) is an edible green alga consumed as a traditional food source in Korea. In this study, the ethanol extract of CF was evaluated to determine if it has anti-inflammatory activity. Lipopolysaccharide (LPS), a toxin from bacteria, is a potent inducer of inflammatory cytokines, such as tumor necrosis factor (TNF)-$\alpha$ and interleukin (IL)-6. Therefore, we studied whether CF extracts have an anti-inflammatory effect in LPS-induced murine macrophage cell lines (RAW 264.7). In the present study, IL-6 production was measured using an enzyme-linked immunosorbent assay (ELISA), prostaglandin $E_2$($PGE_2$) production was measured using the EIA kit, and cyclooxygenase (COX)-2 and mitogen-activated protein kinase (MAPK) activation were determined by Western blot analysis. IL-6 mRNA, COX-2 mRNA and iNOS mRNA expression were measured using reverse transcription-polymerase chain reaction (RT-PCR). The results indicated that CF extracts inhibit LPS-induced IL-6, NO and PGE2 production in a dose-dependent manner, as well as expression of iNOS and COX-2. CF extracts significantly inhibited LPS-induced c-Jun N-terminal kinase (JNK) 1/2 phosphorylation. Taken together, these findings may help elucidate the mechanism by which CF modulates RAW 264.7 cell activation under inflammatory conditions.

• Journal of the korean society of oceanography
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• v.39 no.4
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• pp.234-242
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• 2004

The pigment composition and concentration of phytoplankton assemblages using HPLC in the adjacent waters of four nuclear power plants (Yonggwang, Kori, Wolsong and Ulchin) were investigated during the spring blooming in 2004. The mean concentration of chlorophyll a ranged from 563.8 to 2,949.0ng $l^{-1}$, with the lowest concentration at Kori and the highest concentration at Wolsong. Among the carotenoids, the amounts of fucoxanthin and chlorophyll $C_2$ were relatively higher than those of other pigments in the study site. As minor pigments, zeaxanthin, chlorophyll b, 19'-butanoyloxyfucoxanthin, diadinoxanthin, 19'-hexanoyloxyfucoxanthin, chlorophyll $C_3$ and peridinin were detected. The results of pigment composition and concentration showed that diatoms had an important proportion of phytoplankton community when a spring bloom occurred. Cyanobacteria was present relatively low density at the Wolsong and the green alga such as chlorophytes and prasinophytes were abundant at the Yonggwang and Kori, while dinoflagellates characterized by peridinin were common at Ulchin and Kori. The pigment composition and concentration of phytoplankton after passing through the cooling-water system of nuclear power plant were highly variable. No distinct trend of the change of each pigment composition and amount was detected but the variation of fucoxanthin and chlorophyll $C_2$ highly coupled with that of chlorophyll a. We pointed out that the diatom controlled the overall variation of phytoplankton biomass during the spring season.

• Journal of Marine Bioscience and Biotechnology
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• v.13 no.1
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• pp.1-9
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• 2021

Codium fragile (Suringar) Hariot, a green alga of the Codiales family, has been reported to have several bioactive properties, including antioxidant and anti-inflammatory properties. However, its antiobesity and whitening effects and their underlying mechanisms are unclear. This study aimed to evaluate the antiobesity and melanogenesis inhibitory effects of C. fragile using methanol extracts of C. fragile (MECF). The results of this study revealed that MECF inhibited the accumulation of lipid droplets and triacylglycerol in differentiated 3T3-L1 adipocytes, which was associated with the inhibition of the expression of adipogenesis-related transcription factors, such as peroxisome proliferator-activated receptor γ, CCAAT/enhancer-binding protein-α (C/EBPα), and C/EBPβ, which function as the key regulators of adipogenesis. Also, MECF reduced tyrosinase activity and melanin content in B16F10 cells as well as the expression of tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2, and microphthalmia-related transcription factor in the presence of α-melanocyte-stimulating hormone. Taken together, our findings suggest that the extract of C. fragile could be considered a promising functional ingredient for the prevention and treatment of obesity and skin pigmentation in the food and cosmetic industry.