Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
권호 표지

Bioassay of Spores Attachment for the Weedy Green Alga Ulva fasciata on Agar and Agarose Surfaces

Agar와 Agarose 코팅필름을 이용한 갈파래의 포자 부착 검색

  • Shin, Hyun-Woung (Department of Aquatic Resource Science, College of Natural Science, Soonchunyang University)
  • Published : 1999.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Spore attachment of Ulva fasciata on agar and agaroses coating films showed various surfaces properties according to concentrations of agar or agaroses or via methanol. The highest number of spore attachment occurred in Sigma agarose coating film. Spore attachment on Bacto agar and other agaroses coating films showed the 12 ∼ 36 times less than Sigma agarose coated film. Comparison of spore attachment on 2.5% and 5％ Seakem agarose and Bacto agar coating films differed in two concentrations while coating of 2.5% and 5％ with NuSieve and Sigma agaroses did not differed. Spore attachment of coating made of 5％ and 5% via 4% MeOH with Bacto agar and Nusieve agarose only differed. Overall, these results indicated agar and agarose coating films differed in spore attachment. Results of this work will be useful baseline for bioassay of antifouling activity of fouling organisms.

갈파래의 포자가 부착한 Agar와 Agarose코팅필름은 agar, agarose 또는 메탄놀의 농도에 따라 다양한 표면의 성질을 나타냈다. Sigma agarose 코팅필름에서 가장 많은 수의 포자 부착이 일어났다. Bacto agar과 그외 agarose으로 코팅한 필름에 부착한 포자는 Sigma agarose으로 코팅한 필름보다 12∼36배나 적었다. 2.5%와 5％ 의 SeaKem agarose와 Bacto agar 코팅필름의 비교에서 두 개의 농도 차이가 있는 반면에, 2.5%와 5%의 NuSieve와 Sigma agarose 코팅필름의 비교에서는 차이가 없었다. 5％ 의 Bacto agar와 4％ 의 메탄놀을 포함한 5% Bacto agar 비교, 5％ NuSieve와 4％ 메탄놀을 포함한 5% NuSieve agarose비교에서만 차이를 보였다. 본 연구의 전반적인 결과는 agar와 agarose로 코팅한 필름에서 포자 부착의 차이가 있음을 알 수 있었다. 본연구 결과는 착생생물에 대한 반착생을 검색할 수 있는 기초가 되어질 것이다.

Keywords

References

  1. Bot. Mar. v.24 Marine algae fouling : A review with particular reference to shipfouling Evan LV
  2. In Bioactive Metabolites from microorganisms : Progress in Industrial Microbiology v.27 The discovery of new bioactive microbial metabolites : screening and identification Berdy J;Bushell ME(eds.);U Grafe (eds.)
  3. The chemistry and biology of antibiotics Betina V
  4. Chem. Ind. v.5 Ship fouling : Problems and solution Callow M
  5. Microbiological methods Collins GH;PM Lyne
  6. Biofouling v.10 Minimum effective release rate of antifoulants (2) : Measurement of the effect of TBT and Zosteric acid on hard fouling Haslbeck EG;CJ Kavanagh;HW Shin;WC Banta;P Song;GI Loeb
  7. Phytopathology v.70 Agarose medium for bioassay of antimicrobial sustances Ho WC;Ko WH
  8. J. Nat. Prod. v.52 Antimicrobial and cytotoxic activity of rottlerin-type compounds from Hypericum drummondii Jayasuriya H;JD McChesney;SM Swanson;JM Pezzuto
  9. Methods for research on the ecology of soil-borne plant pathogens Johnson LF;EA Curl
  10. Bioactive natural products : determination, and structural determination Toxicity testing using the brine shrimp : Artemia salina Sam TW;Colegate SM(eds.);RJ Molyneux (eds.)
  11. Kor. J. Phycol. v.10 Biofouling by marine weedy algae Shin HW
  12. Ph. D. dissertation University of Hawaii Ecophysiological studies of reproductive motile cells for the Hawaiian macrophyte, Ulva fasciata (Chlorophyta) Shin HW
  13. Phytochem. v.34 The antifouling activity of natural and synthetic phenolic acid sulphate esters Todd JS;RC Zimmerman;P Crew;RS Alberte
  14. J. Nat. Prod. v.53 Searching for bioactive compounds : various strategies Waterman PG
  15. Bioactive Microbioal products : search and discovery Novel approaches to metabolites screening Zahner H;H Darutx;W Weber;BuLock JD(eds.);LJ Nisbet.(eds.);DJ Winstanley (eds.)
  16. Carbohyd. Res. v.16 The structure of agar. Part Ⅰ. Fraction of a complex mixture of polysaccharides Duckworth M;W Yaphe