• KIEAE Journal
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• v.17 no.4
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• pp.21-32
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• 2017

Purpose: Since 2010, many universities in Korea have been implementing Green Leader Training Programs which are usually run by students themselves. In the last five years of 2015, the Green Leader Training Program under the sponsorship of Korean Association for Green Campus Initiative has promoted the practice of a green campus and many relative projects have been carried out in various fields. Method: We utilized the green campus component index established by the Presidential Committee on Green Growth in Korea and have analyzed 480 activities during the years of 2010~2015. Result: We found that many activities were classified into the environmental field(264, 55.0%). This was followed by university members' participation(130, 27.1%), community engagement(68, 14.2%), and university management(18, 3.7%). These results showed that the green campus initiatives were focused on the environmental field. The number of universities participating from 2010 to 2015 were 20 in the metropolitan area and 15 in the non-metropolitan are. In the metropolitan area, 81.7% of green leader activities were conducted at universities in Seoul, and those in non-capital areas were active in the Middle region(51.3%) and Yeongnam region(48.7%). On the other hand, there was no activity in the Honam region, and it shows that there was a noticeable differential by regional groups.

• ALGAE
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• v.26 no.3
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• pp.229-235
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• 2011

Spliced leader (SL) trans-splicing is a mRNA processing mechanism in dinoflagellate nuclear genes. Although studies have identified a short, conserved dinoflagellate SL (dinoSL) sequence (22-nt) in their nuclear-encoded transcripts, whether the majority of nuclear-coded transcripts in dinoflagellates have the dinoSL sequence remains doubtful. In this study, we investigated dinoSL-containing gene transcripts using 454 pyrosequencing data (Cochlodinium polykrikoides, 93 K sequence reads, 31 Mb; Prorocentrum minimum, 773 K sequence reads, 291 Mb). After making comparisons and performing local BLAST searches, we identified dinoSL for one C. polykrikoides gene transcript and eight P. minimum gene transcripts. This showed transcripts containing the dinoSL sequence were markedly fewer in number than the total expressed sequence tag (EST) transcripts. In addition, we found no direct evidence to prove that most dinoflagellate nuclear-coded transcripts have this dinoSL sequence.

• Journal of fish pathology
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• v.34 no.1
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• pp.17-22
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• 2021

Eukaryotic translation is initiated by either cap-dependent or cap-independent way, and the cap-independent translation can be initiated by the internal ribosomal entry site (IRES). In this study, to know whether the 5'UTR leader sequence of marine birnavirus (MABV) segment A and segment B can act as IRES, bicistronic vectors harboring a CMV promoter-driven red fluorescent gene (mCherry) and poliovirus IRES- or MABV's leader sequence-driven green fluorescent gene (eGFP) were constructed, then, transfected into a mammalian cell line (BHK-21 cells) and a fish cell line (CHSE-214 cells). The results showed that the poliovirus IRES worked well in BHK-21 cells, but did not work in CHSE-214 cells. In the evaluation of MABV's leader sequences, the reporter eGFP gene under the 5'UTR leader sequence of MABV's segment A was well-translated in CHSE-214 cells, indicating 5'UTR of MABV's segment A initiates translation in the cap-independent way and can be used as a fish-specific IRES system. However, the 5'UTR leader sequence of MABV's segment B did not initiate translation in CHSE-214 cells. As the precise mechanism of birnavirid IRES-mediated translation is not known, more elaborate investigations are needed to uncover why the leader sequence of segment B could not initiate translation in the present study. In addition, further studies on the host species range of MABV's segment A IRES and on the screening of other fish-specific IRESs are needed.

• Genomics & Informatics
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• v.15 no.4
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• pp.142-146
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• 2017

More effective production of human insulin is important, because insulin is the main medication that is used to treat multiple types of diabetes and because many people are suffering from diabetes. The current system of insulin production is based on recombinant DNA technology, and the expression vector is composed of a preproinsulin sequence that is a fused form of an artificial leader peptide and the native proinsulin. It has been reported that the sequence of the leader peptide affects the production of insulin. To analyze how the leader peptide affects the maturation of insulin structurally, we adapted several in silico simulations using 13 artificial proinsulin sequences. Three-dimensional structures of models were predicted and compared. Although their sequences had few differences, the predicted structures were somewhat different. The structures were refined by molecular dynamics simulation, and the energy of each model was estimated. Then, protein-protein docking between the models and trypsin was carried out to compare how efficiently the protease could access the cleavage sites of the proinsulin models. The results showed some concordance with experimental results that have been reported; so, we expect our analysis will be used to predict the optimized sequence of artificial proinsulin for more effective production.

• KIEAE Journal
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• v.11 no.5
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• pp.3-12
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• 2011

Industrialization and development of technology satisfied various humanly needs and lay the basis of numerous benefit and profit. New technologies like these mostly required large amounts of fossil Fuel, Fossil energy depletion rate was increasing rapidly. However, technical development for Human race required absolute sacrifice of the environment. Especially, 'City' which had been focused as stage of human activities, allowed to continue to have fossil energy dependent activities, and it shows in many data that the city is responsible for the 75-80% of the green house gas by human. In order to solve the problem relating climate changes and energy, European countries already made progress studies on many of the low carbon green city and pilot case construction. Especially, Germany, Austria, Holland, Sweden who had been playing leader role on environmental awareness and ecological concept, are actively constructing low carbon green city project based on Ecological city planning scheme. These projects positively utilize planning scheme that limits carbon emission using Urban Metabolism concept. Therefore, throughout this study, I would like to present planning and direction of future domestic low carbon green city by analyzing theories relative theories and best practices in Europe.

• Transactions of the Korean hydrogen and new energy society
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• v.35 no.3
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• pp.269-279
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• 2024

Saudi Arabia is transitioning from its traditional oil-dependent economy to become a leader in the hydrogen industry. This paper explores the kingdom's strategic investments in hydrogen production and infrastructure as key components of its energy diversification and sustainability efforts. The focus is on enhancing green hydrogen production to meet domestic needs and strengthen its position in the global market. The urgency of diversifying energy sources is emphasized, with projects like the NEOM Green Hydrogen Project illustrating Saudi Arabia's commitment to renewable energy integration. These initiatives are positioned to centralize Saudi Arabia in the global shift toward sustainable energy, signaling a significant pivot in its economic and environmental policies.

• Hwankyungkyoyuk
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• v.10 no.2
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• pp.249-268
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• 1997

This Study developed 'Environment Management Integrated Leader(EMIL)' program and evaluated the effect of the Program. The procedure of EMIL program development is as follows; 1) Identifying the training needs Through the interview and discussion with corporate environmental staff and research members, training need are collected. 2) Setting the training objectives 30 objectives are developed through specifying and organizing the training needs. 3) Determining the scopes of the program This program consists of the four scopes. First, the scope of Environmentally Acceptable Management is developed based on ISO 14000 and certification of environmentally friendly business. Second, the scope of Environmentally Acceptable Technology includes air pollution, water pollution, solid wasted management, and pollution prevention. Third, the scope of Integrated Environmental Management focuses on master plan, green marketing, green production, and green accounting. Finally, the scope of Integrated Environmental Concepts focuses on food pollution, environmental activities, and bio-diversity. 4) Selecting the training methods Various methods are used such as off-site visits, exercises, case study, workshop, discussion, role-playing as well as lecture. 5) Implementing the program 49 trainees participated the program for 6weeks. All trainees are working for D. Company Group. 6) Measuring training effects To evaluate the effects of the program, pre-post training evaluation method is used. The results are highly significant at all 4 scopes by the p<.01. This study has some implications in order to establish the environmental management climate. First, the development of new environmental education program for management is necessary. Second, Vast investment are required. Third, more systematic approaches are desired in environmental education. Lastly, more customized program development and implementation to fit in industries will accelerate the expansion of environmental concept.

• Korean System Dynamics Review
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• v.15 no.2
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• pp.27-50
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• 2014

Korea government established the energy technology development plan (2011-2020) and declared to be a leader of the green energy technologies. The plan aims for 10% market share in the green energy industry, 12% energy efficiency improvement, and 15% greenhouse gas reduction. In order to achieve these goals, the government has tried to calculate the whole scale of national energy R&D investment, annual budget and specific expenditures for new technologies by computer simulation. The simulation modules include the R&D investment model, GDP model, energy consumption and $CO_2$ emission model by System Dynamics. Based on these simulation modules, I tested various scenarios for effectiveness of energy R&D investments until 2020. The results show that Korea should increase national energy R&D investment to 2.3 billion U.S. dollars, and switch the investment from electricity and nuclear power to the renewable energy.

• Korean Journal of Organic Agriculture
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• v.13 no.1
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• pp.23-51
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• 2005

In this article the author provided a brief introduction to the function and activation of 4-H fanning organization. The strategies developed to improve the environmental-friendly Agriculture are as following : the efficiency cooperatives of 4-H fanning organization, invitation of the upbringing plan of organization leader for promotion of the pilot region project of environmental-friendly Agriculture connected with green tourism, the establishment of reasonable management plan and strategies. In conclusion, there must be strategic measures to increase the income of environmental-friendly farmers. All of this information could be useful for the further development of the strategy for environmental-friendly Agriculture in organization and management.

• Journal of Life Science
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• v.28 no.12
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• pp.1416-1423
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• 2018

With strong biotin binding affinity ($K_D=10^{-14}M$), the tetrameric feature of streptavidin could be used to increase the antigen binding activity of a camel heavy chain (VHH) antibody through their fusion, here stained with biotinylated horseradish peroxidase and subsequent immunoassays ELISA and Western blot analysis. For this application, we cloned the streptavidin gene amplified from the Streptomyces avidinii chromosome by PCR, and this was fused to the gene of the 8B9 VHH antibody which is specific to green fluorescent protein (GFP) antigens. To express a soluble fusion protein in Escherichia coli, we used the pUC119 plasmid-based expression system which uses the lacZ promoter for induction by IPTG, the pelB leader sequence at the N-terminus for secretion into the periplasmic space, and six polyhistidine tags at the C-terminus for purification of the expressed proteins using an $Ni^+$-NTA-agarose column. Although streptavidin is toxic to E. coli because of its strong biotin binding property, this soluble fusion protein was expressed successfully. In SDS-PAGE, the size of the purified fusion protein was 122.4 kDa in its native condition and 30.6 kDa once denatured by boiling, suggesting the tetramerization of the monomeric subunit by non-covalent association through the streptavidin moiety fusing to the 8B9 VHH antibody. In addition, this fusion protein showed biotin binding activity similar to streptavidin as well as GFP antigen binding activity through both ELISA and Western blot analysis. In conclusion, the protein resulting from the fusion of an 8B9 VHH antibody with streptavidin was successfully expressed and purified as a soluble tetramer in E. coli; it showed both biotin and GFP antigen binding activity suggesting the possible production of a tetrameric and bifunctional VHH antibody.