• Korean Journal of Environmental Agriculture
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• v.36 no.3
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• pp.154-160
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• 2017

BACKGROUND:This study was carried out to establish an efficient sample preparation for the simultaneous determination of bisphenols (BPs) in river water samples using gas chromatography-mass spectrometry (GC-MS). Sample preparation was examined with conventional extraction methods, such as solid-phase extraction (SPE) and liquid-liquid extraction (LLE), and their efficiency was compared with validation results, including linearity of calibration curve, method detection limit (MDL), limit of quantification (LOQ), accuracy, and precision. METHODS AND RESULTS:The BPs (bisphenol A, BPA; bisphenol B, BPB; bisphenol C, BPC; bisphenol E, BPE; bisphenol F, BPF; bisphenol S, BPS) were analyzed using GC-MS. The range of MDLs by SPE and LLE methods was $0.0005{\sim}0.0234{\mu}g/L$ and $0.0037{\sim}0.2034{\mu}g/L$, and that of LOQs was $0.0015{\sim}0.0744{\mu}g/L$ and $0.0117{\sim}0.6477{\mu}g/L$, respectively. The calibration curve obtained from standard solution of $0.004{\sim}4.0{\mu}g/L$ (SPE) and $0.016{\sim}16{\mu}g/L$ (LLE) showed good linearity with $r^2$ value of 0.9969 over. Accuracy was 93.2~108% and 97.4~120%, and precision was 1.7~4.6% and 0.7~6.5%, respectively. The values of MDL and LOQ resulted from the SPE method were higher than those from the LLE method, particularly those values of BPA were highest among the BPs. Based on the results, the SPE method was applied to determine the BPs in river water samples. Water samples were collected from mainstream, tributary and sewage wastewater treatment plants (SWTPs) in the Yeongsan river basin. The concentration of BPB, BPC, BPE, BPF and BPS were not detected in all sites, whereas BPA was ranged $0.0095{\sim}0.2583{\mu}g/L$, which was $0.0166{\sim}0.0810{\mu}g/L$ for mainstreams, $0.0095{\sim}0.2583{\mu}g/L$ for tributaries, $0.0352{\sim}0.1217{\mu}g/L$ for SWTPs. CONCLUSION: From these results, the SPE method was very effective for the simultaneous determination of BPs in river water samples using GC-MS. We provided that it is a convenient, reliable and sensitive method enough to monitor and understand the fate of the BPs in aquatic ecosystems.

• Journal of The Korean Society of Inherited Metabolic disease
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• v.17 no.1
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• pp.31-37
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• 2017

Carbamoyl phosphate synthetase 1 deficiency (CPS1D) is an autosomal recessive disorder of the urea cycle that causes hyperammonemia. Two forms of CPS1D are recognized: a lethal neonatal type and a less severe, delayed-onset type. Neonatal CPS1D cases often present their symptoms within the first days of life. Delayed-onset cases are predominantly adolescents or adults, and infantile delayed-onset cases are rare. Severe hyperammonemia in the neonatal period leads to serious brain damage, coma, and death if not treated promptly. Therefore, early diagnosis and acute treatment are crucial. Despite the improvement of treatments, including continuous hemodialysis, ammonia-lowering agents, and a low-protein diet, the overall outcome of severe forms of hyperammonemia often remains disappointing. As the liver is the only organ in which ammonia is converted into urea, liver transplantation has been considered as an elegant and radical alternative therapy to classical dietary and medical therapies. However, liver transplantation has many disadvantages, such as a considerable risk for technical complications and perioperative metabolic derangement, especially in neonates. Additionally, there is a lack of suitable donor organs in most countries. According to recent studies, liver cell transplantation is a therapeutic option and serves as a bridge to liver transplantation. Here, we report a Korean CPS1D patient with novel mutations in CPS1 who was treated by liver cell transplantation after being diagnosed in the neonatal period and showed a good neurodevelopmental outcome at the last follow-up at six months of age.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.33 no.4
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• pp.10-25
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• 2015

The purpose of this research is to investigate the reality behind Jeongjo's enjoyment of the palace garden. If I were to summarize the results of this research, they are as follows: First, The 'Sanghwajoeoyeon(賞花釣魚宴)' has the differentiation which decided and regularized the rules and the regulations with the annual meetings for the Kyujanggak(奎章閣). Jeongjo had held the events five times. Second, The 'Seshimdae(洗心臺) Excursion' is based on the tragic personal history of Jeongjo. There were four events. Third, Jeongjo had enjoyed the 'viewing of the waterfall(觀瀑)' of the Okryucheon(玉流川) where a lot of the water overflows right after it rains. This is worth the special mention with regard to the point that it is a temporary landscape and not an usual landscape. Fourth, As an event for the military officers, the 'Seoljungryonghohwe(雪中龍虎會)' was held seven times. This event had been held in the middle of the winter. And each of the attendees skewered a pheasant and roasted and ate it. Also, it emphasized the sovereign and the subjects being the partners of the joys and the sorrows by giving the alcoholic drinks and the music. Fifth, Jeongjo had consoled the hard work by the civilian subjects either by holding the brazier feast(煖爐會) in the winter or by granting the alcoholic drinks and the foods according to the historical facts of the brazier feasts. Sixth, Jeongjo was good at archery and enjoyed it. During the period of his reign, Jeongjo held twice Yeonsarye(燕射禮). Seventh, Jeongjo soothed his desire to get out of the busy everyday life at the royal palace garden. While appreciating the natural landscape of the backyard, he was awakened of the meaning of the Book of Changes(周易) in which all the things had been created and changed. Eighth, At the royal palace garden, Jeongjo actively prepared the place for enjoyment by the sovereign and the subjects together. It is judged that this is because, in order to materialize his political ideals, the support by all the subjects who had been close was needed. The limitation of this research is the fact that the research had been proceeded with limited to the translations, among the many old writings related to Jeongjo. With the accumulation of the excavations of the new thoughts and of the results of the translations, the researches that are deeper will be needed.

• Journal of Nutrition and Health
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• v.51 no.5
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• pp.455-464
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• 2018

Purpose: This study was conducted to examine the status of dietary behavior and its association with study-related factors in middle school students. Methods: Study-related factors, dietary habit score and dietary behaviors were surveyed by questionnaire and then analyzed. A total of 580 students in some middle schools in Gyeonggi-do participated in this study. Collected data were analyzed using SPSS for windows Ver. 22.0. Results: 71.3% of the subjects slept for 6 ~ 8 hours, and the studying time outside of school was the highest (2 ~ 4 hours). The highest score for stress from studying by private lessons or academies was 'seldom stressed', with higher stress being observed in male students than female students. The average dietary habit score was 3.69 in male students and 3.62 in female students. The highest average time for meal eating was 10 ~ 20 minutes, with shorter times being observed for male students than female students. Eating meals was regular in most students and the highest number of snack eating was 1 ~ 2 times a day. For dietary behaviors by study-related factors, students with more than 2 hours of studying time outside of school showed higher dietary habit scores than those with less than 2 hours, who showed significantly higher rates of eating 2 meals a day. As the sleeping hours and daily average studying hours increased, the rate of regular meal eating increased significantly. Conclusion: The students showed good, above average dietary behaviors, but gender differences in study-related factors and dietary behaviors. Moreover, the sleeping hours and the studying time outside of school were correlated with dietary habit scores and dietary behaviors. Thus, continuous and systematic education for proper dietary behavior is needed along with greater interest in students with problems of study-related factors, particularly those due to study burden.

• The Journal of the Korean bone and joint tumor society
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• v.20 no.1
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• pp.14-21
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• 2014

Purpose: To analyze the clinical features and treatment outcome of Langerhans' cell histocytosis. Materials and Methods: From August 1996 to June 2013, 28 patients who histologically proven with LCH were analyzed of medical records, radiography, pathologic character retrospectively. Results: A total of 28 cases of LCH including 22 child has been reported. Onset age was 0.6 to 51 years old, occurred in the average age was 14.8 years. Follow-up period was 6 months to 134 months average was 44.6 months. The M:F ratio was 2.5:1. The initial symptoms was pain in 18 cases, 5 cases of pathologic fracture, 3 case of palpable mass, 1 case of discovered by accident in radiography, 1 case of torticollis. In radiological examination osteolysis was seen all cases, 7 cases showed a periosteal reaction, 1 case showed soft tissue extension. Clinical type of all cases were eosinophilic granuloma. 25 cases were classified as unifocal disease and 3 cases were multifocal single systemic diseases. In all cases, incisional biopsy was performed. After histologic confirmed, 14 cases was treated with curettage or surgical excision of the lesion and the other 14 cases were followed up without treatment. There is no death during follow up period. 11 cases has no radiological improvement after 3-6 months observation, intralesional steroid injection was performed. Conclusion: Patients with LCH who has rapid systemic onset is very rare, so if you meet the young children who suspected LCH, you shoulder avoid the examination which cause excessive radiation exposure to the young patient. In order to confirm the diagnosis of disease, biopsy is needed. Close observation after confirmed by histological method will bring the satisfactory results. But the patients who had pathologic fracture or wide bone destruction already may need curettage and bone grafting to lesion or internal fixation. The lesion which has no radiological improvement after 3-6 months observation or appear with pain interferes daily life may need local steroid injection as a good treatment.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.12
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• pp.1792-1798
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• 2016

This study investigated quality characteristics, antioxidant activities, and retarding retrogradation of Sulgidduck, traditional rice cake of Korea, prepared using rice flour added with 0%, 1%, 2%, 3%, and 4% pumpkin leaf powder. Moisture content of pumpkin leaf Sulgidduck showed a tendency to increase in the 1% and 2% added group but decrease in the 3% and 4% added group. pH level significantly increased (4.50~6.16) with an increase in content of pumpkin leaf powder (P<0.05). For color, as content of pumpkin leaf powder increased, L-value decreased and b-value increased. Hardness tended to increase with addition of pumpkin leaf powder except for the 1% added group. Springiness was not significantly different (P<0.05). Chewiness and adhesiveness increased according to addition level increase. Retarding retrogradation showed that the pumpkin leaf powder added group had a stronger effect compared to the control, whereas the 2% added group presented the most retarding retrogradation effect with the lowest Avrami exponent. Total phenols content and ABTS radical scavenging activity tended to increase as content of pumpkin leaf powder increased. Sensory properties by 7-point scale test showed that the 2% added group had the highest scores, whereas the 4% added group had the lowest scores. These results suggest that pumpkin leaf is considered as a good material to improve quality characteristics, antioxidant activities, and retarding retrogradation of Sulgidduck. The most appropriate proportion for the greatest effect of retarding retrogradation in Sulgidduck was considered to be 2%.

• Journal of Dairy Science and Biotechnology
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• v.16 no.2
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• pp.90-97
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• 1998

This study was carried out to investigate changes of microbiological and sensory properties in various heat-treated market milks (LTLT, HTST, and UHT milks) stored at 10$^{\circ}C$ during 15d. Titratable acidity (TA) increased with storage, while pH tended to decrease. During the initial 9d, no difference was found in TA, however, after 9d, it was slightly higher in HTST and UHT milks than that in LTLT milk. In LTLT and HTST milks, total viable cells and psychrotrophs were dramatically increased during storage, In addition coliform and pathogenic bacteria were found at 12 and 15d. In UHT milk, total viable cells were found only at 15d. In sensory evaluation, LTLT and HTST milks developed a negligible off-flavor until 9d. At 12d, it became stronger in HTST milk than that in LTLT milk. In UHT milk, off-flavor was detected at 9d and increased rapidly there-after. The degree of off-flavor was little higher in HTST and UHT milks, compared with that of LTLT milk after 9d storage. These observations indicated that LTLT and HTST milks may not be microbiologically acceptable after 5d, while off-flavor was not detectable until 9d, In comparison, UHT milk keeps a good quality in microorganism until 15d, however, it may not be accepted in sensory aspect after 9d storage.

• Korean Journal of Ecology and Environment
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• v.40 no.1
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• pp.130-142
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• 2007

This study was to examine impacts of turbid water on fish community in the downstream of Yongdam Dam during the period from June to October 2006. For the research, we selected six sampling sites in the field: two sites were controls with no influences of turbid water from the dam and other remaining four sites were the stations for an assessment of potential turbid effects. We evaluated integrative health conditions throughout applications of various models such as necropsy-based fish health assessment model (FHA), Index of Biological Integrity (IBI) using fish assemblages, and Qualitative Habitat Evaluation Index (QHEI). Laboratory tests on fish exposure under 400 NTU were performed to find out impact of turbid water using scanning electron microscope (SEM). Results showed that fine solid particles were clogging in the gill in the treatments, while particles were not found in the control. This results indicate that when inorganic turbidity increases abruptedly, fish may have a mechanical abrasion or respiratory blocking. The stream health condition, based on the IBI values, ranged between 38 and 48 (average: 42), indicating a "excellent" or "good" condition after the criteria of US EPA (1993). In the mean time, physical habitat condition, based on the QHEI, ranged 97 to 187 (average 154), indicating a "suboptimal condition". These biological outcomes were compared with chemical dataset: IBI values were more correlated (r=0.526, p<0.05, n=18) with QHEI rather than chemical water quality, based on turbidity (r=0.260, p>0.05, n=18). Analysis of the FHA showed that the individual health indicated "excellent condition", while QHEI showed no habitat disturbances (especially bottom substrate and embeddeness), food-web, and spawning place. Consequently, we concluded that the ecological health in downstream of Yongdam Dam was not impacted by the turbid water.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.4
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• pp.279-286
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• 2002

This pot experiment was conducted to find out the forage productivity and contents of mineral nutrients in an orchardgrass sward affected by the compost lime, and phosphorus applications on the newly reclaimed and arable pasture soils. This 2nd part was concerned with the effect of lime application(L$_{0}$=control, L$_1$=279, L$_2$=54g CaCO$_3$/pot). The results obtained are summarized as follows: 1. There were considerable differences between the newly reclaimed and arable pasture soils in the soil chemical properties as follows in general; poor/common in the newly reclaimed soil longrightarrow and common/good in the arable pasture soil in terms of soil fertility standard. 2. It was recognized that the lime application was resulted in somewhat decrease of the concentrations of available P$_2$O$_{5}$ and increase of P$_2$O$_{5}$ absorbtion coefficient in the newly reclaimed soil, whereas these negative effects were positively enhanced by both lime and compost applications. The concentrations of exchangeable Ca and mg in both soils were increased by lime application. 3. The vigour at early growth stage of orchardgrass was better in the arable pasture soil than newly reclaimed soil. It was worsened by the heavy lime application(L$_2$), but this negative effect was positively enhanced by both lime and compost application. 4. Compared with the non-lime application, the forage yields of L$_1$ treatment were increased in both soils by 15~18%. whereas it of L$_2$ treatment was similar or lower than it of L$_1$. 5. The concentrations of Ca, mg, and P in orchardgrass were relatively higher in the arable pasture soil than the newly reclaimed soil, whereas there it was no difference in the K concentration. It was recognized that the concentrations of mg and Ca in orchardgrass were higher in the L$_1$ treatment than the Lo treatment in both soils, whereas there were no difference in it between L$_1$ and L$_2$ treatments.ments.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.6
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• pp.496-502
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• 2004

The development of sesame varieties which contain high contents of lignan compounds has been progressed in Korea. This study was carried out to get the basic information for the breeding of high quality sesame varieties and the development of health functional food sources using lignan compounds from sesame. The contents of sesamin and sesamolin were $4.08\pm1.74$ and $2.47\pm0.68mg/g$, respectively, from sesame cultivated in 2002 and those were $4.08\pm1.51$ and $2.48\pm0.69mg/g$, respectively, from sesame cultivated in 2003. The content of sesamine was high in the order of Danbaek (6.22 mg/g), Seongbun (5.94 mg/g), Namda (5.83 mg/g), and Naman (5.59 mg/g) producted in 2002 and Seongbun (6.27 mg/g), Dasak (5.53 mg/g), Danbaek (5.50 mg/g), chinbaek (5.45 mg/g), and Seodun (5.41 mg/g) producted in 2003. The content of sesmoline was high in the order of Hwangbaek (3.27 mg/g), Seongbun (3.26 mg/g), Annam (3.22 mg/g), Hanseom (3.17 mg/g), and Danbaek (3.16 mg/g) producted in 2002, and Seongbun (3.21 mg/g), Seodun (3.14 mg/g), Pungan (3.10 mg/g), Naman (3.09 mg/g), and Danbaek (3.04 mg/g) producted in 2003. The contents of sesamin and sesamolin of Yangbaek, which has used as a standard variety for sesame breeding, were relatively lower than in any other varieties. These resluts suggest that Yangbaek is not the proper variety as a standard variety for development of high quality sesame. In conclusion, proper good variety as a standard variety for sesame breeding must be determined after careful consideration on the major quality factors including crude oil and lignan compounds as well as agricultural characteristics to develop high quality sesame varieties containing high contents of lignan compounds.