• Korean Journal of Fisheries and Aquatic Sciences
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• v.18 no.3
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• pp.253-261
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• 1985

The reproduction of marbled sole Limanda yokohamae, caught near around the southeastern coast of Korea, from December 1983 to November 1984, was investigated based on such annual variations as gonadosomatic index(GSL), gametogenesis, reproductive cycle, spawning number, hepatosomatic index (HSI), and fatness. GSI began to increase in the autumn season with the onset of shorter day length and colder water temperature, and reached the maximum value in December with the shortest day length and the lowest temperature over the year. The gonad activated the proliferation of oogonia and spermatogonia in June, reached the mature stage in October, ripe in December, and spawning from the end of December to January. After spawning, it showed the resting stage which gonad remained regressive and suppressive from February to May. In addition, the adult individuals observed discharged eggs only once during their spawning period. At yolk globular stage, the substance of vitellogenin synthesized from the liver was considered to participate in the active yolk accumulation of oocytes. Marbled sole was concluded to be a typical winter spawning species in that such environmental factors as short day length and low water temperature were closely related with the gametogenesis, the stimulation of oocyte maturation, and were also affecting the ovulation.

• Journal of Aquaculture
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• v.9 no.3
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• pp.205-213
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• 1996

Experiments were carried out to investigate the effect of GnRH-analogue (GnRH-a) on the induction of ovulation in catfish, S. asotus. Fully matured female catfish ($250\~600\;g$) received a single intraperitoneal injection of GnRH-a ($50\~200\;{\mu}g/kg{\cdot}body$ weight) showed the successful induction of ovulation. More than $86\%$ of treated females were ovulated after injection of GnRH-a ($90\;{\mu}g/kg$) at $25{\pm}1^{\circ}C$. The majority of spawning took place within 22 to 25 hours after the injection. The gonadosomatic index (GSI) and pseudo-GSI in the group treated with $120\;{\mu}g/kg$ GnRH-a were $23-30\%$ and $18-21\%$, respectively. Average fertilization and hatching rates were $94\%\;and\;81\%$, respectively. Electron microscopically, gonadotrophs of maturing female catfish were characterized by the presence of numerous small, electron-dense granules of approximately $150\~300$ nm in diameter and a few larger, less electron-dense granules of approximately $800\~1000$ nm in size in their cytoplasm. Gonadotrophs of GnRH-a treated catfish showed that their was a distinct decrease in number of small and large granules. The rough endoplasmic reticulum was composed of numerous cisternae conspicuosly dilated to various degrees.

• Journal of Aquaculture
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• v.17 no.1
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• pp.30-38
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• 2004

To estimate the spawning period the annual change of gonadosomatic index (GSI) were examined from January 2000 to December 2001. Fecundity, spawning frequency and egg diameter were measured by ocular observation. Germ cell differentiation during gametogenesis, the reproductive cycle and the first sexual maturity of greenling Hexagrammos otakii were observed under light microscopy from January to December, 2000. GSI began to increase in August and reached the maximum in November when ovary was getting mature. The reproductive cycle of H. otakii can be divided into five successive stages in females: early growing stage (July), late growing stage (July to August), mature stage (September to October), ripe and spent stage (September to December), and recovery and resting stage (December to June). Males showed four successive stages : growing (June to August), mature (August to October), ripe and spent (September to December), and recovery and resting stage (December to May). According to the frequency distributions of egg diameter in spawning season, H. otakii could be one of polycyclic species spawning 2 times or more during one spawning season. Number of total eggs and mature eggs in the absolute fecundity were related to the standard length and body weight, respectively. Number of total eggs and mature eggs in relative fecundity were also proportional to the standard length, but rather these numbers decreased with body weight. Percentages of first sexual maturity of females and males in greenling were over 50% from 19.1 to 21.1cm in length, and 100% for fish over 25.1cm in length. Therefore, both sexes are ready to reproduce after one year old.

• Korean Journal of Ichthyology
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• v.20 no.3
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• pp.179-189
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• 2008

Standard length (SL) and gonadosomatic index (GSI) of Plecoglossus altivelis were investigated from March to October 2007 in Seomjin River and streams flowing to the East Sea, Korea. Water temperatures were $17{\sim}23^{\circ}C$ during the spawning season, which ran from late September to early November in Seomjin River and early August to mid-October in streams flowing to the East Sea. The GSI and HSI variations of ayu population in Seomjin River increased earlier than in populations in streams flowing to the East Sea with similar variation in water temperature. Histological study of ovaries showed that the start of spawning of P. altivelis in Seomjin River was later than in streams flowing to the East Sea. Overall, fecundity increased with increase in SL and not by site specificity. The SL of P. altivelis increased as the river scale increased. Mean length of specimens from Seomjin River (river length: 223.86 km, river area: $4,911.89km^2$) was $194{\pm}15.3mm$, from Osip Stream (55.76 km, $393.78km^2$) $185{\pm}15.5mm$, from Yangyang Namdae Stream (54 km, $474.08km^2$) $142{\pm}11.8mm$, and from Joosoo Stream (21.1 km, $141.47km^2$) $136{\pm}16.7 mm$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.45 no.3
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• pp.253-261
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• 2012

We investigated the reproductive capacity of starry flounder Platichthys stellatus from the Uljin (UJ) marine ranching area and compared it to that of P. stellatus from the coastal waters of Pohang (PH). In UJ, female gonadosomatic index (GSI) peaked in October ($3.14{\pm}0.87$) and male GSI was high in October and December. In PH, female GSI peaked in January ($18.64{\pm}2.15$) while male GSI began to increase in October and remained high until March. Most ovaries of UJ females were immature with perinucleus oocytes, although the testes of UJ males were ripe in January. Both the ovaries and testes of PH starry flounders were ripe from January to March. The plasma estradiol-$17{\beta}$ ($E_2$) levels of UJ females were highest in October ($4.09{\pm}1.90$ ng/mL) although the testosterone (T) levels of UJ males were highest in December ($3.81{\pm}0.78$ ng/mL) and decreased gradually until April. The $17{\alpha}$,$20{\beta}$-dihydroxy-4-pregnen-3-one ($17{\alpha}20{\beta}P$) levels of UJ females were not detected. The E2 levels of PH females were highest in December ($36.25{\pm}33.07$ ng/mL) and $17{\alpha}20{\beta}P$ levels were highest in March ($5.51{\pm}0.95$5 ng/mL). The T levels of PH males were highest in December ($4.03{\pm}1.34$ ng/mL) and decreased gradually until October. Taken together, these results suggest that most females from UJ did not reach maturation with a spawning period that was considered to be between December and January.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.5
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• pp.404-409
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• 2006

This study investigated the reproductive cycle of the female hairychin goby, Sagamia geneionema, histologically. The fecundity of female hairychin goby ranged from 1,002 to 1,240 eggs when they reached a total length of 9.1-10.0 cm. Fecundity is related to total length. The gonadosomatic index (GSI) increased from December (1.87$\pm$0.46) and reached a maximum in April (11.57$\pm$1.92). The histological changes in the ovary were correlated with the GSI. Oocytes at the chromatin-nucleolus and peri-nucleolus stages were observed in the ovary year-round. In December, oocytes containing yolk appeared in the ovaries of a few fish. Most oocytes appearing in January were at the yolk globule stage. The frequency of oocytes appearing at the yolk globule stage from January to March was higher than in other months. Subsequently, empty follicles and atretic oocytes were observed in the ovaries in May. Based on the histological observations of gonad development and the monthly change in the GSI, the reproductive cycle was classified into the following successive stages: growing (October to November), mature (December to January), ripe and spawning (February to April), and degenerative and resting (May to September) stages. The histological observations of ovaries during the spawning period indicate that this species is a multiple spawner with abbreviated iteroparity based on the developmental pattern of oocytes.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.24 no.3
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• pp.167-176
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• 1991

Sexual maturation of the bluespotted mud hopper, Boleophthalmus Pectinirostris(Linnaeus) was investigated histologically on the gonadal development, and studied by gonadosomatic index(GSI), egg diameter composition. Samples were collected in the intertidal zone of Wolyon-ri, Hoihyon-myon, Okku-gun, Chollabuk-do, Korea, from April to October in 1988 and from June to August in 1989. The ovary is a pair of sac-shaped organ. The testis is a pair of tubule-shaped organ and it is connected to the seminal vesicle which is located at the posterior end of the testis. In male and female, GSI began to increase from late May when the water temperature began to increase and reached the maximum value in June and July, respectively. It began to decrease from August, the highest water temperature season. Thereafter, maintained relatively low values until October. The annual reproductive cycle of this species could be classified into four sucessive developmental stages: growing stage$(April{\~}May)$, mature stage$(June{\~}early\;July)$, ripe and spent stage(late lune-early August), degenerative and resting stage$(late\;August{\~}March:\;the wintering\;period)$. According to the frequency distributions of egg diameters in the spawning season, Boleophthalmus Pectinirostris was species to spawn twice or more in the spawning season.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.4
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• pp.489-499
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• 1998

Gonadal development, reproductive cycle and embryonic development of the marbled rockfish, Sebastiscus marmoratus were investigated histologically. A total of 1,035 specimens were collected from the Cheju Island from July of 1992 to May of 1996. Gonadosomatic index (GSI) in female reached the maximum during October to March, thereafter, the values decreased from Norvember to April. GSI in male reached the maximum in September (before one to three months of the female's maximum), thereafter, the values rapidly decreased from October to December. The annual reproductive cycle can be divided into five stages in female and four stages in male: females, Growing(June to September), Mature(September to February), Ripe and copulation(October to March), Gestation and parturition(November to April), Degenerative and resting(December to May); in males, Growing(April to July), Mature(August to November), Ripe and copulation(September to December), and Degenerstive and resting(November to March). Size frequency distribution of eggs and larvae showed non-synchrony. Maternal larvae parturition occurred one or two time during November to April. Mean length of the larvae parturition was 3.5 mm. Mean number of eggs and maternal larvae for the 15 cm minimum class of female was 58,377, and fecundity increased with the increase of total length and body weight.

• The Korean Journal of Malacology
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• v.22 no.1 s.35
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• pp.13-22
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• 2006

Gonadosomatic index (GSI), oogenesis and reproductive cycle of Glossaulax didyma were investigated by the cytological histological obserbations and morphometric data. Samples were collected monthly from the intertidal zone of Biin Bay, Seocheon, Korea, for one year. Monthly variations in the GSI showed similar patterns with gonadal development. In the early vitellogenic oocyte, the Golgi complex and mitochondria were invovled in the formation of lipid droplet and yolk precursor. In the late vitellogenic oocytes, the rough endoplasmic reticulum and multivesicular bodies were involved in the formation of profeid yolk granules in the cytoplasm. A mature yolk granule was composed of three components: main body (central core), superficial layer, and the limiting membrane. The spawning season was from early June to late August, and the main spawning occurred between July and August when the seawater temperature was above $19^{\circ}C$. The female reproductive cycle can be classified into five successive stages: early active stage (December to February), late active stage (February to March), ripe stage (April to July), spawning stage (June to August), recovery stage (August to November). Fully mature oocytes were approximately $250-270{\mu}m$ in diameter.

• Development and Reproduction
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• v.12 no.3
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• pp.261-266
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• 2008

In the present study, we investigated in vivo effects of Manchurian trout recombinant gonadotrophin hormone (mt-rGTH) on the induction of maturation in female eel, Anguilla japonica. The brood stock, female eel (450$\pm$50 g) were weekly injected intramuscularly with different doses of 0.1, 1, 10 ${\mu}g\m{\ell}$/fish (mt-rFSH or mt-rLH) for 10 week. The effects of r-mtGTH were analyzed by gonadosomatic index (GSI), ovarian follicle diameter and sex steroid levels. All groups did not exhibit significant differences in the GSI values. Whereas plasma testosterone (T) and estradiol-17$\beta$ (E2) levels did not change significantly in control group, plasma levels of T and E2 by injection of the r-mtFSH or r-mtLH were increased at 2 or 4 week after injection. In addition, injection of the mt-rFSH (1, 10 ${\mu}g/m{\ell}$/fish) or mt-rLH (0.1, 1, 10 ${\mu}g/m{\ell}$/fish) significantly increased follicle diameters comparing to the control group. These results demonstrate that the recombinant hormone may affect early ovary development and maturation in female eel. Taken together, these results suggest that the recombinant Manchurian trout FSH and LH are effective for reproductive activities in female eel.